Role of glycocalyx in leukocyte-endothelial cell adhesion
Department of Bioengineering, Pennsylvania State University, University Park, Pennsylvania 16802 The binding of fluorescently labeled microspheres (FLMs, 0.1-µm diameter) coated with antibody (1a29) to ICAM-1 was studied in postcapillary venules during topical application of the chemoattractant N -f...
Gespeichert in:
| Veröffentlicht in: | American journal of physiology. Heart and circulatory physiology 2002-10, Vol.283 (4), p.H1282-H1291 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | H1291 |
|---|---|
| container_issue | 4 |
| container_start_page | H1282 |
| container_title | American journal of physiology. Heart and circulatory physiology |
| container_volume | 283 |
| creator | Mulivor, A. W Lipowsky, H. H |
| description | Department of Bioengineering, Pennsylvania State
University, University Park, Pennsylvania 16802
The
binding of fluorescently labeled microspheres (FLMs, 0.1-µm diameter)
coated with antibody (1a29) to ICAM-1 was studied in postcapillary
venules during topical application of the chemoattractant N -formylmethionyl-leucyl-phenylalanine (fMLP). FLM adhesion
to endothelial cells (ECs) increased dramatically from 50 to 150 spheres per 100-µm length of venule after superfusion of the
mesentery with fMLP and equaled or exceeded levels of leukocyte (WBC)
adhesion. Removal of the EC glycocalyx by micropipette infusion of the
venule with heparinase increased FLM-EC adhesion to levels attained
with fMLP. Subsequent application of fMLP did not increase FLM adhesion further, suggesting that the FLMs saturated all ICAM-1 binding sites.
Perfusion with heparinase after suffusion with fMLP significantly increased FLM-EC adhesion above levels attained with fMLP. However, WBC
adhesion fell because of possible removal of selectins necessary to
maintain WBC rolling at the wall. It is concluded that the glycocalyx
serves as a barrier to adhesion and that its shedding during natural
activation of ECs may be an essential part of the inflammatory response.
endothelium; heparinase; N -formylmethionyl-leucyl-phenylalanine |
| doi_str_mv | 10.1152/ajpheart.00117.2002 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72103650</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72103650</sourcerecordid><originalsourceid>FETCH-LOGICAL-c504t-dbae960360dae3d669660aa782c1eb4081de7ad95ec08c1c4db99adfd915cfac3</originalsourceid><addsrcrecordid>eNp1kE9LwzAYh4Mobk4_gSA9eeuWP23T4knEOWEgyDyHNHm7dmZNbVpcv72tm86Lpxzyex5eHoSuCZ4SEtKZ3FQ5yLqZYkwIn1KM6Qka9z_UJyFLTtEYs4j5EWHhCF04t8EYhzxi52hEKGUB53yMkldrwLOZtzadskqabucVpWegfbeqa8CHUtsmB1NI4ykwxpM6B1fY8hKdZdI4uDq8E_Q2f1w9LPzly9Pzw_3SVyEOGl-nEpKovwRrCUxHURJFWEoeU0UgDXBMNHCpkxAUjhVRgU6TROpMJyRUmVRsgm733qq2Hy24RmwLN1wiS7CtE5yS3h7ifsj2Q1Vb52rIRFUXW1l3gmAxFBM_xcR3MTEU66mbg75Nt6CPzCFRP5jtB3mxzj-LGkSVd30AY9fd0UhjJgKxIDQelHf_E_PWmBXsml_0DykqnbEvZfmQeQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72103650</pqid></control><display><type>article</type><title>Role of glycocalyx in leukocyte-endothelial cell adhesion</title><source>MEDLINE</source><source>American Physiological Society</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Mulivor, A. W ; Lipowsky, H. H</creator><creatorcontrib>Mulivor, A. W ; Lipowsky, H. H</creatorcontrib><description>Department of Bioengineering, Pennsylvania State
University, University Park, Pennsylvania 16802
The
binding of fluorescently labeled microspheres (FLMs, 0.1-µm diameter)
coated with antibody (1a29) to ICAM-1 was studied in postcapillary
venules during topical application of the chemoattractant N -formylmethionyl-leucyl-phenylalanine (fMLP). FLM adhesion
to endothelial cells (ECs) increased dramatically from 50 to 150 spheres per 100-µm length of venule after superfusion of the
mesentery with fMLP and equaled or exceeded levels of leukocyte (WBC)
adhesion. Removal of the EC glycocalyx by micropipette infusion of the
venule with heparinase increased FLM-EC adhesion to levels attained
with fMLP. Subsequent application of fMLP did not increase FLM adhesion further, suggesting that the FLMs saturated all ICAM-1 binding sites.
Perfusion with heparinase after suffusion with fMLP significantly increased FLM-EC adhesion above levels attained with fMLP. However, WBC
adhesion fell because of possible removal of selectins necessary to
maintain WBC rolling at the wall. It is concluded that the glycocalyx
serves as a barrier to adhesion and that its shedding during natural
activation of ECs may be an essential part of the inflammatory response.
endothelium; heparinase; N -formylmethionyl-leucyl-phenylalanine</description><identifier>ISSN: 0363-6135</identifier><identifier>EISSN: 1522-1539</identifier><identifier>DOI: 10.1152/ajpheart.00117.2002</identifier><identifier>PMID: 12234777</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Antibody Specificity ; Cell Adhesion - drug effects ; Cell Adhesion - immunology ; Endothelium, Vascular - cytology ; Endothelium, Vascular - immunology ; Glycocalyx - immunology ; Heparin Lyase - pharmacology ; Intercellular Adhesion Molecule-1 - immunology ; Leukocytes - cytology ; Male ; Microspheres ; N-Formylmethionine Leucyl-Phenylalanine - pharmacology ; Rats ; Rats, Sprague-Dawley ; Venules - cytology ; Venules - immunology</subject><ispartof>American journal of physiology. Heart and circulatory physiology, 2002-10, Vol.283 (4), p.H1282-H1291</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-dbae960360dae3d669660aa782c1eb4081de7ad95ec08c1c4db99adfd915cfac3</citedby><cites>FETCH-LOGICAL-c504t-dbae960360dae3d669660aa782c1eb4081de7ad95ec08c1c4db99adfd915cfac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,3028,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12234777$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mulivor, A. W</creatorcontrib><creatorcontrib>Lipowsky, H. H</creatorcontrib><title>Role of glycocalyx in leukocyte-endothelial cell adhesion</title><title>American journal of physiology. Heart and circulatory physiology</title><addtitle>Am J Physiol Heart Circ Physiol</addtitle><description>Department of Bioengineering, Pennsylvania State
University, University Park, Pennsylvania 16802
The
binding of fluorescently labeled microspheres (FLMs, 0.1-µm diameter)
coated with antibody (1a29) to ICAM-1 was studied in postcapillary
venules during topical application of the chemoattractant N -formylmethionyl-leucyl-phenylalanine (fMLP). FLM adhesion
to endothelial cells (ECs) increased dramatically from 50 to 150 spheres per 100-µm length of venule after superfusion of the
mesentery with fMLP and equaled or exceeded levels of leukocyte (WBC)
adhesion. Removal of the EC glycocalyx by micropipette infusion of the
venule with heparinase increased FLM-EC adhesion to levels attained
with fMLP. Subsequent application of fMLP did not increase FLM adhesion further, suggesting that the FLMs saturated all ICAM-1 binding sites.
Perfusion with heparinase after suffusion with fMLP significantly increased FLM-EC adhesion above levels attained with fMLP. However, WBC
adhesion fell because of possible removal of selectins necessary to
maintain WBC rolling at the wall. It is concluded that the glycocalyx
serves as a barrier to adhesion and that its shedding during natural
activation of ECs may be an essential part of the inflammatory response.
endothelium; heparinase; N -formylmethionyl-leucyl-phenylalanine</description><subject>Animals</subject><subject>Antibody Specificity</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Adhesion - immunology</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - immunology</subject><subject>Glycocalyx - immunology</subject><subject>Heparin Lyase - pharmacology</subject><subject>Intercellular Adhesion Molecule-1 - immunology</subject><subject>Leukocytes - cytology</subject><subject>Male</subject><subject>Microspheres</subject><subject>N-Formylmethionine Leucyl-Phenylalanine - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Venules - cytology</subject><subject>Venules - immunology</subject><issn>0363-6135</issn><issn>1522-1539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE9LwzAYh4Mobk4_gSA9eeuWP23T4knEOWEgyDyHNHm7dmZNbVpcv72tm86Lpxzyex5eHoSuCZ4SEtKZ3FQ5yLqZYkwIn1KM6Qka9z_UJyFLTtEYs4j5EWHhCF04t8EYhzxi52hEKGUB53yMkldrwLOZtzadskqabucVpWegfbeqa8CHUtsmB1NI4ykwxpM6B1fY8hKdZdI4uDq8E_Q2f1w9LPzly9Pzw_3SVyEOGl-nEpKovwRrCUxHURJFWEoeU0UgDXBMNHCpkxAUjhVRgU6TROpMJyRUmVRsgm733qq2Hy24RmwLN1wiS7CtE5yS3h7ifsj2Q1Vb52rIRFUXW1l3gmAxFBM_xcR3MTEU66mbg75Nt6CPzCFRP5jtB3mxzj-LGkSVd30AY9fd0UhjJgKxIDQelHf_E_PWmBXsml_0DykqnbEvZfmQeQ</recordid><startdate>20021001</startdate><enddate>20021001</enddate><creator>Mulivor, A. W</creator><creator>Lipowsky, H. H</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20021001</creationdate><title>Role of glycocalyx in leukocyte-endothelial cell adhesion</title><author>Mulivor, A. W ; Lipowsky, H. H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-dbae960360dae3d669660aa782c1eb4081de7ad95ec08c1c4db99adfd915cfac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Antibody Specificity</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Adhesion - immunology</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - immunology</topic><topic>Glycocalyx - immunology</topic><topic>Heparin Lyase - pharmacology</topic><topic>Intercellular Adhesion Molecule-1 - immunology</topic><topic>Leukocytes - cytology</topic><topic>Male</topic><topic>Microspheres</topic><topic>N-Formylmethionine Leucyl-Phenylalanine - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Venules - cytology</topic><topic>Venules - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mulivor, A. W</creatorcontrib><creatorcontrib>Lipowsky, H. H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of physiology. Heart and circulatory physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mulivor, A. W</au><au>Lipowsky, H. H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of glycocalyx in leukocyte-endothelial cell adhesion</atitle><jtitle>American journal of physiology. Heart and circulatory physiology</jtitle><addtitle>Am J Physiol Heart Circ Physiol</addtitle><date>2002-10-01</date><risdate>2002</risdate><volume>283</volume><issue>4</issue><spage>H1282</spage><epage>H1291</epage><pages>H1282-H1291</pages><issn>0363-6135</issn><eissn>1522-1539</eissn><abstract>Department of Bioengineering, Pennsylvania State
University, University Park, Pennsylvania 16802
The
binding of fluorescently labeled microspheres (FLMs, 0.1-µm diameter)
coated with antibody (1a29) to ICAM-1 was studied in postcapillary
venules during topical application of the chemoattractant N -formylmethionyl-leucyl-phenylalanine (fMLP). FLM adhesion
to endothelial cells (ECs) increased dramatically from 50 to 150 spheres per 100-µm length of venule after superfusion of the
mesentery with fMLP and equaled or exceeded levels of leukocyte (WBC)
adhesion. Removal of the EC glycocalyx by micropipette infusion of the
venule with heparinase increased FLM-EC adhesion to levels attained
with fMLP. Subsequent application of fMLP did not increase FLM adhesion further, suggesting that the FLMs saturated all ICAM-1 binding sites.
Perfusion with heparinase after suffusion with fMLP significantly increased FLM-EC adhesion above levels attained with fMLP. However, WBC
adhesion fell because of possible removal of selectins necessary to
maintain WBC rolling at the wall. It is concluded that the glycocalyx
serves as a barrier to adhesion and that its shedding during natural
activation of ECs may be an essential part of the inflammatory response.
endothelium; heparinase; N -formylmethionyl-leucyl-phenylalanine</abstract><cop>United States</cop><pmid>12234777</pmid><doi>10.1152/ajpheart.00117.2002</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0363-6135 |
| ispartof | American journal of physiology. Heart and circulatory physiology, 2002-10, Vol.283 (4), p.H1282-H1291 |
| issn | 0363-6135 1522-1539 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72103650 |
| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Antibody Specificity Cell Adhesion - drug effects Cell Adhesion - immunology Endothelium, Vascular - cytology Endothelium, Vascular - immunology Glycocalyx - immunology Heparin Lyase - pharmacology Intercellular Adhesion Molecule-1 - immunology Leukocytes - cytology Male Microspheres N-Formylmethionine Leucyl-Phenylalanine - pharmacology Rats Rats, Sprague-Dawley Venules - cytology Venules - immunology |
| title | Role of glycocalyx in leukocyte-endothelial cell adhesion |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T12%3A47%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Role%20of%20glycocalyx%20in%20leukocyte-endothelial%20cell%20adhesion&rft.jtitle=American%20journal%20of%20physiology.%20Heart%20and%20circulatory%20physiology&rft.au=Mulivor,%20A.%20W&rft.date=2002-10-01&rft.volume=283&rft.issue=4&rft.spage=H1282&rft.epage=H1291&rft.pages=H1282-H1291&rft.issn=0363-6135&rft.eissn=1522-1539&rft_id=info:doi/10.1152/ajpheart.00117.2002&rft_dat=%3Cproquest_cross%3E72103650%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72103650&rft_id=info:pmid/12234777&rfr_iscdi=true |