Fluorescence resonance energy transfer mapping of the fourth of six nucleotide-binding sites of chloroplast coupling factor 1

Equilibrium dialysis measurements of adenine nucleotide binding to chloroplast coupling factor 1 suggest that the enzyme has six binding sites for ADP, adenylyl-beta,gamma-imidodiphosphate (AMP-PNP), and 2'(3')-O-2,4,6-trinitrophenyl-ATP (TNP-ATP). High affinity binding at all six sites re...

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Veröffentlicht in:	The Journal of biological chemistry 1991-09, Vol.266 (26), p.17276-17285
Hauptverfasser:	Shapiro, A.B. (Cornell University, Ithaca, NY), Gibson, K.D, Scheraga, H.A, McCarty, R.E
Format:	Artikel
Sprache:	eng
Schlagworte:	ADENOSINDIFOSFATO ADENOSINE DIPHOSPHATE Adenosine Diphosphate - metabolism ADENOSINE MONOPHOSPHATE ADENOSINE TRIPHOSPHATE Adenosine Triphosphate - analogs & derivatives Adenosine Triphosphate - metabolism ADENOSINMONOFOSFATO ADENOSINTRIFOSFATO Adenylyl Imidodiphosphate - metabolism ADP Analytical, structural and metabolic biochemistry Binding Sites Biological and medical sciences CHLOROPLASTE Chromatography, High Pressure Liquid CLOROPLASTO Energy Transfer Enzymes and enzyme inhibitors ESPECTROMETRIA Fluorescent Dyes Fundamental and applied biological sciences. Psychology H super(+)-transporting ATP synthase HIDROLASAS HYDROLASE Hydrolases Magnesium - metabolism PROPIEDADES OPTICAS PROPRIETE OPTIQUE Proton-Translocating ATPases - metabolism SPECTROMETRIE Spectrometry, Fluorescence
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