Noradrenaline-induced prostaglandin production by sympathetic postganglionic neurons is mediated by alpha 2-adrenergic receptors
In this study we have demonstrated that noradrenaline increases the levels of prostaglandin E2 and prostaglandin I2 (detected as the stable metabolite 6-keto-prostaglandin F1 alpha) synthesized by homogenates of superior cervical ganglia from the adult rat. This noradrenaline-induced prostaglandin p...
Gespeichert in:
| Veröffentlicht in: | Journal of neurochemistry 1991-10, Vol.57 (4), p.1145-1150 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1150 |
|---|---|
| container_issue | 4 |
| container_start_page | 1145 |
| container_title | Journal of neurochemistry |
| container_volume | 57 |
| creator | Gonzales, R Sherbourne, C D Goldyne, M E Levine, J D |
| description | In this study we have demonstrated that noradrenaline increases the levels of prostaglandin E2 and prostaglandin I2 (detected as the stable metabolite 6-keto-prostaglandin F1 alpha) synthesized by homogenates of superior cervical ganglia from the adult rat. This noradrenaline-induced prostaglandin production was further characterized: (a) Selective destruction of adrenergic sympathetic postganglionic neurons in the ganglia using 6-hydroxydopamine abolished both basal and stimulated prostaglandin production. (b) Elimination of preganglionic cholinergic sympathetic nerve terminals in the ganglia had no effect. (c) Mepacrine (a phospholipase inhibitor) and indomethacin (a cyclooxygenase inhibitor) attenuated both basal and stimulated prostaglandin production. (d) Yohimbine, but not prazosin, suppressed the noradrenaline dose-response curve for prostaglandin production. The results of these experiments show that, in vitro, noradrenaline stimulates de novo synthesis of prostaglandin E2 and prostaglandin I2 by sympathetic postganglionic neurons. This stimulation by noradrenaline appears to result from action at an alpha 2-adrenergic receptor. |
| doi_str_mv | 10.1111/j.1471-4159.1991.tb08272.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72103210</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72103210</sourcerecordid><originalsourceid>FETCH-LOGICAL-c246t-6d47c06ce2ee52e1e10153963ffcf6f8efee4c2402799b43faf4343f8af2078a3</originalsourceid><addsrcrecordid>eNpFkE1LAzEQhoMotVZ_grB48LZrvrof3qT4BUUveg7Z7KRN2WbXJAvtzZ9u1hYNhGFm3neGeRC6ITgj8d1tMsILknIyrzJSVSQLNS5pQbPdCZr-tU7RFGNKU4Y5PUcX3m8wJjnPyQRNSD7nrCym6Putc7JxYGVrLKTGNoOCJuld54NctdI2xo5ZLAfT2aTeJ36_7WVYQzAq6aNsJe2qjb2YWhhcZ31ifLKFxsgQR0WHbPu1TGj6uwjcKiodKOhD5_wlOtOy9XB1jDP0-fT4sXhJl-_Pr4uHZaooz0OaN7xQOFdAAeYUCBBM5qzKmdZK57oEDcCjFNOiqmrOtNScxVBKTXFRSjZDt4e58ZivAXwQW-MVtPFE6AYvCkowG_8M3R-EKjLwDrTondlKtxcEixG_2IiRsRgZixG_OOIXu2i-Pm4Z6kjg33rgzX4AnVKGhg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72103210</pqid></control><display><type>article</type><title>Noradrenaline-induced prostaglandin production by sympathetic postganglionic neurons is mediated by alpha 2-adrenergic receptors</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Gonzales, R ; Sherbourne, C D ; Goldyne, M E ; Levine, J D</creator><creatorcontrib>Gonzales, R ; Sherbourne, C D ; Goldyne, M E ; Levine, J D</creatorcontrib><description>In this study we have demonstrated that noradrenaline increases the levels of prostaglandin E2 and prostaglandin I2 (detected as the stable metabolite 6-keto-prostaglandin F1 alpha) synthesized by homogenates of superior cervical ganglia from the adult rat. This noradrenaline-induced prostaglandin production was further characterized: (a) Selective destruction of adrenergic sympathetic postganglionic neurons in the ganglia using 6-hydroxydopamine abolished both basal and stimulated prostaglandin production. (b) Elimination of preganglionic cholinergic sympathetic nerve terminals in the ganglia had no effect. (c) Mepacrine (a phospholipase inhibitor) and indomethacin (a cyclooxygenase inhibitor) attenuated both basal and stimulated prostaglandin production. (d) Yohimbine, but not prazosin, suppressed the noradrenaline dose-response curve for prostaglandin production. The results of these experiments show that, in vitro, noradrenaline stimulates de novo synthesis of prostaglandin E2 and prostaglandin I2 by sympathetic postganglionic neurons. This stimulation by noradrenaline appears to result from action at an alpha 2-adrenergic receptor.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.1991.tb08272.x</identifier><identifier>PMID: 1654387</identifier><language>eng</language><publisher>England</publisher><subject>6-Ketoprostaglandin F1 alpha - biosynthesis ; Animals ; Denervation ; Dinoprostone - biosynthesis ; Epoprostenol - biosynthesis ; Female ; Ganglia, Sympathetic - cytology ; Ganglia, Sympathetic - metabolism ; Male ; Neurons - metabolism ; Norepinephrine - pharmacology ; Prazosin - pharmacology ; Quinacrine - pharmacology ; Rats ; Receptors, Adrenergic, alpha - physiology ; Yohimbine - pharmacology</subject><ispartof>Journal of neurochemistry, 1991-10, Vol.57 (4), p.1145-1150</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c246t-6d47c06ce2ee52e1e10153963ffcf6f8efee4c2402799b43faf4343f8af2078a3</citedby><cites>FETCH-LOGICAL-c246t-6d47c06ce2ee52e1e10153963ffcf6f8efee4c2402799b43faf4343f8af2078a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1654387$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gonzales, R</creatorcontrib><creatorcontrib>Sherbourne, C D</creatorcontrib><creatorcontrib>Goldyne, M E</creatorcontrib><creatorcontrib>Levine, J D</creatorcontrib><title>Noradrenaline-induced prostaglandin production by sympathetic postganglionic neurons is mediated by alpha 2-adrenergic receptors</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>In this study we have demonstrated that noradrenaline increases the levels of prostaglandin E2 and prostaglandin I2 (detected as the stable metabolite 6-keto-prostaglandin F1 alpha) synthesized by homogenates of superior cervical ganglia from the adult rat. This noradrenaline-induced prostaglandin production was further characterized: (a) Selective destruction of adrenergic sympathetic postganglionic neurons in the ganglia using 6-hydroxydopamine abolished both basal and stimulated prostaglandin production. (b) Elimination of preganglionic cholinergic sympathetic nerve terminals in the ganglia had no effect. (c) Mepacrine (a phospholipase inhibitor) and indomethacin (a cyclooxygenase inhibitor) attenuated both basal and stimulated prostaglandin production. (d) Yohimbine, but not prazosin, suppressed the noradrenaline dose-response curve for prostaglandin production. The results of these experiments show that, in vitro, noradrenaline stimulates de novo synthesis of prostaglandin E2 and prostaglandin I2 by sympathetic postganglionic neurons. This stimulation by noradrenaline appears to result from action at an alpha 2-adrenergic receptor.</description><subject>6-Ketoprostaglandin F1 alpha - biosynthesis</subject><subject>Animals</subject><subject>Denervation</subject><subject>Dinoprostone - biosynthesis</subject><subject>Epoprostenol - biosynthesis</subject><subject>Female</subject><subject>Ganglia, Sympathetic - cytology</subject><subject>Ganglia, Sympathetic - metabolism</subject><subject>Male</subject><subject>Neurons - metabolism</subject><subject>Norepinephrine - pharmacology</subject><subject>Prazosin - pharmacology</subject><subject>Quinacrine - pharmacology</subject><subject>Rats</subject><subject>Receptors, Adrenergic, alpha - physiology</subject><subject>Yohimbine - pharmacology</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1LAzEQhoMotVZ_grB48LZrvrof3qT4BUUveg7Z7KRN2WbXJAvtzZ9u1hYNhGFm3neGeRC6ITgj8d1tMsILknIyrzJSVSQLNS5pQbPdCZr-tU7RFGNKU4Y5PUcX3m8wJjnPyQRNSD7nrCym6Putc7JxYGVrLKTGNoOCJuld54NctdI2xo5ZLAfT2aTeJ36_7WVYQzAq6aNsJe2qjb2YWhhcZ31ifLKFxsgQR0WHbPu1TGj6uwjcKiodKOhD5_wlOtOy9XB1jDP0-fT4sXhJl-_Pr4uHZaooz0OaN7xQOFdAAeYUCBBM5qzKmdZK57oEDcCjFNOiqmrOtNScxVBKTXFRSjZDt4e58ZivAXwQW-MVtPFE6AYvCkowG_8M3R-EKjLwDrTondlKtxcEixG_2IiRsRgZixG_OOIXu2i-Pm4Z6kjg33rgzX4AnVKGhg</recordid><startdate>199110</startdate><enddate>199110</enddate><creator>Gonzales, R</creator><creator>Sherbourne, C D</creator><creator>Goldyne, M E</creator><creator>Levine, J D</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199110</creationdate><title>Noradrenaline-induced prostaglandin production by sympathetic postganglionic neurons is mediated by alpha 2-adrenergic receptors</title><author>Gonzales, R ; Sherbourne, C D ; Goldyne, M E ; Levine, J D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c246t-6d47c06ce2ee52e1e10153963ffcf6f8efee4c2402799b43faf4343f8af2078a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>6-Ketoprostaglandin F1 alpha - biosynthesis</topic><topic>Animals</topic><topic>Denervation</topic><topic>Dinoprostone - biosynthesis</topic><topic>Epoprostenol - biosynthesis</topic><topic>Female</topic><topic>Ganglia, Sympathetic - cytology</topic><topic>Ganglia, Sympathetic - metabolism</topic><topic>Male</topic><topic>Neurons - metabolism</topic><topic>Norepinephrine - pharmacology</topic><topic>Prazosin - pharmacology</topic><topic>Quinacrine - pharmacology</topic><topic>Rats</topic><topic>Receptors, Adrenergic, alpha - physiology</topic><topic>Yohimbine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gonzales, R</creatorcontrib><creatorcontrib>Sherbourne, C D</creatorcontrib><creatorcontrib>Goldyne, M E</creatorcontrib><creatorcontrib>Levine, J D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gonzales, R</au><au>Sherbourne, C D</au><au>Goldyne, M E</au><au>Levine, J D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Noradrenaline-induced prostaglandin production by sympathetic postganglionic neurons is mediated by alpha 2-adrenergic receptors</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1991-10</date><risdate>1991</risdate><volume>57</volume><issue>4</issue><spage>1145</spage><epage>1150</epage><pages>1145-1150</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><abstract>In this study we have demonstrated that noradrenaline increases the levels of prostaglandin E2 and prostaglandin I2 (detected as the stable metabolite 6-keto-prostaglandin F1 alpha) synthesized by homogenates of superior cervical ganglia from the adult rat. This noradrenaline-induced prostaglandin production was further characterized: (a) Selective destruction of adrenergic sympathetic postganglionic neurons in the ganglia using 6-hydroxydopamine abolished both basal and stimulated prostaglandin production. (b) Elimination of preganglionic cholinergic sympathetic nerve terminals in the ganglia had no effect. (c) Mepacrine (a phospholipase inhibitor) and indomethacin (a cyclooxygenase inhibitor) attenuated both basal and stimulated prostaglandin production. (d) Yohimbine, but not prazosin, suppressed the noradrenaline dose-response curve for prostaglandin production. The results of these experiments show that, in vitro, noradrenaline stimulates de novo synthesis of prostaglandin E2 and prostaglandin I2 by sympathetic postganglionic neurons. This stimulation by noradrenaline appears to result from action at an alpha 2-adrenergic receptor.</abstract><cop>England</cop><pmid>1654387</pmid><doi>10.1111/j.1471-4159.1991.tb08272.x</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3042 |
| ispartof | Journal of neurochemistry, 1991-10, Vol.57 (4), p.1145-1150 |
| issn | 0022-3042 1471-4159 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72103210 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | 6-Ketoprostaglandin F1 alpha - biosynthesis Animals Denervation Dinoprostone - biosynthesis Epoprostenol - biosynthesis Female Ganglia, Sympathetic - cytology Ganglia, Sympathetic - metabolism Male Neurons - metabolism Norepinephrine - pharmacology Prazosin - pharmacology Quinacrine - pharmacology Rats Receptors, Adrenergic, alpha - physiology Yohimbine - pharmacology |
| title | Noradrenaline-induced prostaglandin production by sympathetic postganglionic neurons is mediated by alpha 2-adrenergic receptors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T18%3A35%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Noradrenaline-induced%20prostaglandin%20production%20by%20sympathetic%20postganglionic%20neurons%20is%20mediated%20by%20alpha%202-adrenergic%20receptors&rft.jtitle=Journal%20of%20neurochemistry&rft.au=Gonzales,%20R&rft.date=1991-10&rft.volume=57&rft.issue=4&rft.spage=1145&rft.epage=1150&rft.pages=1145-1150&rft.issn=0022-3042&rft.eissn=1471-4159&rft_id=info:doi/10.1111/j.1471-4159.1991.tb08272.x&rft_dat=%3Cproquest_cross%3E72103210%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=72103210&rft_id=info:pmid/1654387&rfr_iscdi=true |