Use of tetracycline in larval diet to study the effect of Wolbachia on host fecundity and clarify taxonomic status of Trichogramma species in cured bisexual lines
Endosymbionts of the genus Wolbachia were efficiently cured from Trichogramma species by incorporating 0.02% tetracycline into the artificial diet used to rear larvae. Use of this technique yielded stable cured lines (bisexual and arrhenotokous lines) in which no Wolbachia organisms were detected by...
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| Veröffentlicht in: | Journal of invertebrate pathology 2002-05, Vol.80 (1), p.13-21 |
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| creator | Grenier, Simon Gomes, Silvia M Pintureau, Bernard Lassablière, François Bolland, Patrice |
| description | Endosymbionts of the genus
Wolbachia were efficiently cured from
Trichogramma species by incorporating 0.02% tetracycline into the artificial diet used to rear larvae. Use of this technique yielded stable cured lines (bisexual and arrhenotokous lines) in which no
Wolbachia organisms were detected by PCR for up to 14 generations after curing. Four cured strains of
Trichogramma pretiosum showed a significantly lower total fecundity compared to their
Wolbachia-infected counterpart. However, the fecundity of a single cured strain of
Trichogramma evanescens was similar to its
Wolbachia-infected counterpart. These differences in the effect on fecundity may be due to differences between the
Wolbachia strains infecting
T. pretiosum or
T. evanescens, providing additional evidence for the hypothesis that a specific interaction exists between some
Trichogramma species and their
Wolbachia symbionts. Tetracycline in larval diet was also used to generate bisexual strains of
Trichogramma oleae and
Trichogramma cordubensis so that these species could be crossed with the closely related species, respectively,
T. pretiosum and
T. evanescens, to test their compatibility. These crosses showed a lack of compatibility, validating maintenance of these as distinct species. |
| doi_str_mv | 10.1016/S0022-2011(02)00039-3 |
| format | Article |
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Wolbachia were efficiently cured from
Trichogramma species by incorporating 0.02% tetracycline into the artificial diet used to rear larvae. Use of this technique yielded stable cured lines (bisexual and arrhenotokous lines) in which no
Wolbachia organisms were detected by PCR for up to 14 generations after curing. Four cured strains of
Trichogramma pretiosum showed a significantly lower total fecundity compared to their
Wolbachia-infected counterpart. However, the fecundity of a single cured strain of
Trichogramma evanescens was similar to its
Wolbachia-infected counterpart. These differences in the effect on fecundity may be due to differences between the
Wolbachia strains infecting
T. pretiosum or
T. evanescens, providing additional evidence for the hypothesis that a specific interaction exists between some
Trichogramma species and their
Wolbachia symbionts. Tetracycline in larval diet was also used to generate bisexual strains of
Trichogramma oleae and
Trichogramma cordubensis so that these species could be crossed with the closely related species, respectively,
T. pretiosum and
T. evanescens, to test their compatibility. These crosses showed a lack of compatibility, validating maintenance of these as distinct species.</description><identifier>ISSN: 0022-2011</identifier><identifier>EISSN: 1096-0805</identifier><identifier>DOI: 10.1016/S0022-2011(02)00039-3</identifier><identifier>PMID: 12234537</identifier><identifier>CODEN: JIVPAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animal and plant ecology ; Animal, plant and microbial ecology ; Animals ; Anti-Bacterial Agents - pharmacology ; Autoecology ; Bacteria Rickettsiaceae ; Biological and medical sciences ; Crosses, Genetic ; Egg parasitoid ; Endosymbiont ; Fecundity ; Female ; Fertility - drug effects ; Fundamental and applied biological sciences. Psychology ; Hymenoptera Trichogrammatidae ; Larva - drug effects ; Larva - microbiology ; Male ; Protozoa. Invertebrata ; Taxonomy ; Tetracycline - pharmacology ; Thelytoky/arrhenotoky ; Wasps - drug effects ; Wasps - microbiology ; Wolbachia - physiology ; Wolbachia cured strain</subject><ispartof>Journal of invertebrate pathology, 2002-05, Vol.80 (1), p.13-21</ispartof><rights>2002 Elsevier Science (USA)</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-81dcb86d21acf11f13b795c980b96c39ffaad28f7b5f817adffa572856e24ed3</citedby><cites>FETCH-LOGICAL-c422t-81dcb86d21acf11f13b795c980b96c39ffaad28f7b5f817adffa572856e24ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022201102000393$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13944067$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12234537$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Grenier, Simon</creatorcontrib><creatorcontrib>Gomes, Silvia M</creatorcontrib><creatorcontrib>Pintureau, Bernard</creatorcontrib><creatorcontrib>Lassablière, François</creatorcontrib><creatorcontrib>Bolland, Patrice</creatorcontrib><title>Use of tetracycline in larval diet to study the effect of Wolbachia on host fecundity and clarify taxonomic status of Trichogramma species in cured bisexual lines</title><title>Journal of invertebrate pathology</title><addtitle>J Invertebr Pathol</addtitle><description>Endosymbionts of the genus
Wolbachia were efficiently cured from
Trichogramma species by incorporating 0.02% tetracycline into the artificial diet used to rear larvae. Use of this technique yielded stable cured lines (bisexual and arrhenotokous lines) in which no
Wolbachia organisms were detected by PCR for up to 14 generations after curing. Four cured strains of
Trichogramma pretiosum showed a significantly lower total fecundity compared to their
Wolbachia-infected counterpart. However, the fecundity of a single cured strain of
Trichogramma evanescens was similar to its
Wolbachia-infected counterpart. These differences in the effect on fecundity may be due to differences between the
Wolbachia strains infecting
T. pretiosum or
T. evanescens, providing additional evidence for the hypothesis that a specific interaction exists between some
Trichogramma species and their
Wolbachia symbionts. Tetracycline in larval diet was also used to generate bisexual strains of
Trichogramma oleae and
Trichogramma cordubensis so that these species could be crossed with the closely related species, respectively,
T. pretiosum and
T. evanescens, to test their compatibility. These crosses showed a lack of compatibility, validating maintenance of these as distinct species.</description><subject>Animal and plant ecology</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Autoecology</subject><subject>Bacteria Rickettsiaceae</subject><subject>Biological and medical sciences</subject><subject>Crosses, Genetic</subject><subject>Egg parasitoid</subject><subject>Endosymbiont</subject><subject>Fecundity</subject><subject>Female</subject><subject>Fertility - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hymenoptera Trichogrammatidae</subject><subject>Larva - drug effects</subject><subject>Larva - microbiology</subject><subject>Male</subject><subject>Protozoa. Invertebrata</subject><subject>Taxonomy</subject><subject>Tetracycline - pharmacology</subject><subject>Thelytoky/arrhenotoky</subject><subject>Wasps - drug effects</subject><subject>Wasps - microbiology</subject><subject>Wolbachia - physiology</subject><subject>Wolbachia cured strain</subject><issn>0022-2011</issn><issn>1096-0805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhSMEokPhEUDegGARuHbi_KwQqmhBqsSCQSytG_uaMUriwXaqzuvwpDidEV12Zcv-zjlX9xTFSw7vOfDmw3cAIUoBnL8F8Q4Aqr6sHhUbDn1TQgfycbH5j5wVz2L8Dfkmm_5pccaFqGpZtZvi749IzFuWKAXUBz26mZib2YjhBkdmHCWWPItpMQeWdsTIWtJplfz044B655D5me18TCz_LLNx6cBwNkxnD2ezCm_97CenswumJa7abXB6538FnCZkcU_aUVxj9RLIsMFFul1y_DpNfF48sThGenE6z4vt5eftxZfy-tvV14tP16WuhUhlx40eusYIjtpybnk1tL3UfQdD3-iqtxbRiM62g7Qdb9HkB9mKTjYkajLVefHmaLsP_s9CManJRU3jiDP5JapWcBBSwoMg7-oWBDQZlEdQBx9jIKv2wU0YDoqDWktUdyWqtSEFQt2VqKqse3UKWIaJzL3q1FoGXp8AjBpHG3DWLt5zVV_X0KzcxyNHeW03joKKedGzJuNCLlEZ7x4Y5R99ibwO</recordid><startdate>20020501</startdate><enddate>20020501</enddate><creator>Grenier, Simon</creator><creator>Gomes, Silvia M</creator><creator>Pintureau, Bernard</creator><creator>Lassablière, François</creator><creator>Bolland, Patrice</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7SS</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20020501</creationdate><title>Use of tetracycline in larval diet to study the effect of Wolbachia on host fecundity and clarify taxonomic status of Trichogramma species in cured bisexual lines</title><author>Grenier, Simon ; Gomes, Silvia M ; Pintureau, Bernard ; Lassablière, François ; Bolland, Patrice</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-81dcb86d21acf11f13b795c980b96c39ffaad28f7b5f817adffa572856e24ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animal and plant ecology</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Autoecology</topic><topic>Bacteria Rickettsiaceae</topic><topic>Biological and medical sciences</topic><topic>Crosses, Genetic</topic><topic>Egg parasitoid</topic><topic>Endosymbiont</topic><topic>Fecundity</topic><topic>Female</topic><topic>Fertility - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hymenoptera Trichogrammatidae</topic><topic>Larva - drug effects</topic><topic>Larva - microbiology</topic><topic>Male</topic><topic>Protozoa. Invertebrata</topic><topic>Taxonomy</topic><topic>Tetracycline - pharmacology</topic><topic>Thelytoky/arrhenotoky</topic><topic>Wasps - drug effects</topic><topic>Wasps - microbiology</topic><topic>Wolbachia - physiology</topic><topic>Wolbachia cured strain</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grenier, Simon</creatorcontrib><creatorcontrib>Gomes, Silvia M</creatorcontrib><creatorcontrib>Pintureau, Bernard</creatorcontrib><creatorcontrib>Lassablière, François</creatorcontrib><creatorcontrib>Bolland, Patrice</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of invertebrate pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grenier, Simon</au><au>Gomes, Silvia M</au><au>Pintureau, Bernard</au><au>Lassablière, François</au><au>Bolland, Patrice</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of tetracycline in larval diet to study the effect of Wolbachia on host fecundity and clarify taxonomic status of Trichogramma species in cured bisexual lines</atitle><jtitle>Journal of invertebrate pathology</jtitle><addtitle>J Invertebr Pathol</addtitle><date>2002-05-01</date><risdate>2002</risdate><volume>80</volume><issue>1</issue><spage>13</spage><epage>21</epage><pages>13-21</pages><issn>0022-2011</issn><eissn>1096-0805</eissn><coden>JIVPAZ</coden><abstract>Endosymbionts of the genus
Wolbachia were efficiently cured from
Trichogramma species by incorporating 0.02% tetracycline into the artificial diet used to rear larvae. Use of this technique yielded stable cured lines (bisexual and arrhenotokous lines) in which no
Wolbachia organisms were detected by PCR for up to 14 generations after curing. Four cured strains of
Trichogramma pretiosum showed a significantly lower total fecundity compared to their
Wolbachia-infected counterpart. However, the fecundity of a single cured strain of
Trichogramma evanescens was similar to its
Wolbachia-infected counterpart. These differences in the effect on fecundity may be due to differences between the
Wolbachia strains infecting
T. pretiosum or
T. evanescens, providing additional evidence for the hypothesis that a specific interaction exists between some
Trichogramma species and their
Wolbachia symbionts. Tetracycline in larval diet was also used to generate bisexual strains of
Trichogramma oleae and
Trichogramma cordubensis so that these species could be crossed with the closely related species, respectively,
T. pretiosum and
T. evanescens, to test their compatibility. These crosses showed a lack of compatibility, validating maintenance of these as distinct species.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>12234537</pmid><doi>10.1016/S0022-2011(02)00039-3</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of invertebrate pathology, 2002-05, Vol.80 (1), p.13-21 |
| issn | 0022-2011 1096-0805 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72102550 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animal and plant ecology Animal, plant and microbial ecology Animals Anti-Bacterial Agents - pharmacology Autoecology Bacteria Rickettsiaceae Biological and medical sciences Crosses, Genetic Egg parasitoid Endosymbiont Fecundity Female Fertility - drug effects Fundamental and applied biological sciences. Psychology Hymenoptera Trichogrammatidae Larva - drug effects Larva - microbiology Male Protozoa. Invertebrata Taxonomy Tetracycline - pharmacology Thelytoky/arrhenotoky Wasps - drug effects Wasps - microbiology Wolbachia - physiology Wolbachia cured strain |
| title | Use of tetracycline in larval diet to study the effect of Wolbachia on host fecundity and clarify taxonomic status of Trichogramma species in cured bisexual lines |
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