Carbachol induces secretion in a mast cell line (RBL-2H3) transfected with the ml muscarinic receptor gene
The possibility that the ml muscarinic receptor subtype can induce release of intracellular granules and transmitters was studied by transfecting a cultured mast cell line, RBL-2H3 cells, with the ml receptor gene. Comparisons were made between carbachol- and antigen-induced activation of various se...
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| Veröffentlicht in: | FEBS letters 1991-09, Vol.289 (1), p.47-50 |
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| creator | Jones, S.V.Penelope Choi, Oksoon H. Beaven, Michael A. |
| description | The possibility that the ml muscarinic receptor subtype can induce release of intracellular granules and transmitters was studied by transfecting a cultured mast cell line, RBL-2H3 cells, with the ml receptor gene. Comparisons were made between carbachol- and antigen-induced activation of various secretory responses. Like antigen, carbachol stimulated inositol phospholipid hydrolysis and release of arachidonic acid with concomitant dose-dependent secretion of granular contents. Carbachol also stimulated a biphasic increase in intracellular calcium, as measured by single cell fura-2 measurements. Although the kinetics of the carbachol-induced rise in intracellular calcium differed from that induced by antigen, they both utilized the same intracellular pool of calcium, and the second phase of the rise in intracellular calcium was dependent on extracellular calcium in both cases. Thus, the ml muscarinic receptor activates release of granules by a mechanism ostensibly similar to that of antigen. |
| doi_str_mv | 10.1016/0014-5793(91)80905-I |
| format | Article |
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Comparisons were made between carbachol- and antigen-induced activation of various secretory responses. Like antigen, carbachol stimulated inositol phospholipid hydrolysis and release of arachidonic acid with concomitant dose-dependent secretion of granular contents. Carbachol also stimulated a biphasic increase in intracellular calcium, as measured by single cell fura-2 measurements. Although the kinetics of the carbachol-induced rise in intracellular calcium differed from that induced by antigen, they both utilized the same intracellular pool of calcium, and the second phase of the rise in intracellular calcium was dependent on extracellular calcium in both cases. Thus, the ml muscarinic receptor activates release of granules by a mechanism ostensibly similar to that of antigen.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(91)80905-I</identifier><identifier>PMID: 1832647</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>[Ca2+]i ; acetylcholine ; Aminoquinolines ; Animals ; Antigens - pharmacology ; Arachidonic Acid ; Arachidonic Acids - metabolism ; beta-N-Acetylhexosaminidases - metabolism ; Biological and medical sciences ; Calcium - metabolism ; carbachol ; Carbachol - pharmacology ; Cell Line ; Cell physiology ; Cloned ml muscarinic receptor ; concentration of free cytosolic Ca2 ; dinitrophenylated bovine serum albumin ; DNP-BSA ; EGTA ; ethylene glycol-bis-(β aminoethylether) N,N,N′N′-tetraacetic acid ; Exocytosis ; Expression (RBL-2H3 cell) ; Fluorescent Dyes ; Fundamental and applied biological sciences. Psychology ; Hydrolysis ; IgE ; immunoglobulin E ; Inositol Phosphates - metabolism ; Intracellular signal ; Mast Cells - metabolism ; Molecular and cellular biology ; Phospholipids - metabolism ; Rats ; Receptors, Muscarinic - genetics ; Receptors, Muscarinic - metabolism ; Secretion. Exocytosis ; Transfection</subject><ispartof>FEBS letters, 1991-09, Vol.289 (1), p.47-50</ispartof><rights>1991</rights><rights>FEBS Letters 289 (1991) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c395I-a11c7f876c99c41828ec190dea77ea64811dc6ca1b4d15a92d4c8a21218072153</citedby><cites>FETCH-LOGICAL-c395I-a11c7f876c99c41828ec190dea77ea64811dc6ca1b4d15a92d4c8a21218072153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/001457939180905I$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65308</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5039293$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1832647$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jones, S.V.Penelope</creatorcontrib><creatorcontrib>Choi, Oksoon H.</creatorcontrib><creatorcontrib>Beaven, Michael A.</creatorcontrib><title>Carbachol induces secretion in a mast cell line (RBL-2H3) transfected with the ml muscarinic receptor gene</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The possibility that the ml muscarinic receptor subtype can induce release of intracellular granules and transmitters was studied by transfecting a cultured mast cell line, RBL-2H3 cells, with the ml receptor gene. Comparisons were made between carbachol- and antigen-induced activation of various secretory responses. Like antigen, carbachol stimulated inositol phospholipid hydrolysis and release of arachidonic acid with concomitant dose-dependent secretion of granular contents. Carbachol also stimulated a biphasic increase in intracellular calcium, as measured by single cell fura-2 measurements. Although the kinetics of the carbachol-induced rise in intracellular calcium differed from that induced by antigen, they both utilized the same intracellular pool of calcium, and the second phase of the rise in intracellular calcium was dependent on extracellular calcium in both cases. Thus, the ml muscarinic receptor activates release of granules by a mechanism ostensibly similar to that of antigen.</description><subject>[Ca2+]i</subject><subject>acetylcholine</subject><subject>Aminoquinolines</subject><subject>Animals</subject><subject>Antigens - pharmacology</subject><subject>Arachidonic Acid</subject><subject>Arachidonic Acids - metabolism</subject><subject>beta-N-Acetylhexosaminidases - metabolism</subject><subject>Biological and medical sciences</subject><subject>Calcium - metabolism</subject><subject>carbachol</subject><subject>Carbachol - pharmacology</subject><subject>Cell Line</subject><subject>Cell physiology</subject><subject>Cloned ml muscarinic receptor</subject><subject>concentration of free cytosolic Ca2</subject><subject>dinitrophenylated bovine serum albumin</subject><subject>DNP-BSA</subject><subject>EGTA</subject><subject>ethylene glycol-bis-(β aminoethylether) N,N,N′N′-tetraacetic acid</subject><subject>Exocytosis</subject><subject>Expression (RBL-2H3 cell)</subject><subject>Fluorescent Dyes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolysis</subject><subject>IgE</subject><subject>immunoglobulin E</subject><subject>Inositol Phosphates - metabolism</subject><subject>Intracellular signal</subject><subject>Mast Cells - metabolism</subject><subject>Molecular and cellular biology</subject><subject>Phospholipids - metabolism</subject><subject>Rats</subject><subject>Receptors, Muscarinic - genetics</subject><subject>Receptors, Muscarinic - metabolism</subject><subject>Secretion. Exocytosis</subject><subject>Transfection</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQQC0EKkvhH4DkA0LtIeBJnMS-INFVl660EhKCs-UdT1hX-VjshKr_HqdZlRtwsjzzPON5w9hrEO9BQPVBCJBZWeviQsOlElqU2fYJW4Gqi6yQlXrKVo_Ic_YixluR7gr0GTsDVeSVrFfsdm3D3uJhaLnv3YQUeSQMNPqhTxFueWfjyJHalre-J37x9WqX5TfFJR-D7WNDOJLjd3488PFAvGt5N0W0wfceeSCk4zgE_oN6esmeNbaN9Op0nrPvm-tv65ts9-Xzdv1pl2Ghy21mAbBuVF2h1ihB5YoQtHBk65psJRWAwwot7KWD0urcSVQ2hxyUqHMoi3P2bql7DMPPieJoOh_nAWxPwxRNgpIGCf8EoRKyVLJIoFxADEOMgRpzDL6z4d6AMPMuzCzazKKNBvOwC7NNz96c6k_7jtyfR4v8lH97yttkrG2ST_TxEStFoXM9d98s2J1v6f6_WpvN9VU-J-a4hofo_J-PSyFK9n95Ciaipx7J-bSo0bjB_32g37m5txI</recordid><startdate>19910902</startdate><enddate>19910902</enddate><creator>Jones, S.V.Penelope</creator><creator>Choi, Oksoon H.</creator><creator>Beaven, Michael A.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19910902</creationdate><title>Carbachol induces secretion in a mast cell line (RBL-2H3) transfected with the ml muscarinic receptor gene</title><author>Jones, S.V.Penelope ; Choi, Oksoon H. ; Beaven, Michael A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c395I-a11c7f876c99c41828ec190dea77ea64811dc6ca1b4d15a92d4c8a21218072153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>[Ca2+]i</topic><topic>acetylcholine</topic><topic>Aminoquinolines</topic><topic>Animals</topic><topic>Antigens - pharmacology</topic><topic>Arachidonic Acid</topic><topic>Arachidonic Acids - metabolism</topic><topic>beta-N-Acetylhexosaminidases - metabolism</topic><topic>Biological and medical sciences</topic><topic>Calcium - metabolism</topic><topic>carbachol</topic><topic>Carbachol - pharmacology</topic><topic>Cell Line</topic><topic>Cell physiology</topic><topic>Cloned ml muscarinic receptor</topic><topic>concentration of free cytosolic Ca2</topic><topic>dinitrophenylated bovine serum albumin</topic><topic>DNP-BSA</topic><topic>EGTA</topic><topic>ethylene glycol-bis-(β aminoethylether) N,N,N′N′-tetraacetic acid</topic><topic>Exocytosis</topic><topic>Expression (RBL-2H3 cell)</topic><topic>Fluorescent Dyes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrolysis</topic><topic>IgE</topic><topic>immunoglobulin E</topic><topic>Inositol Phosphates - metabolism</topic><topic>Intracellular signal</topic><topic>Mast Cells - metabolism</topic><topic>Molecular and cellular biology</topic><topic>Phospholipids - metabolism</topic><topic>Rats</topic><topic>Receptors, Muscarinic - genetics</topic><topic>Receptors, Muscarinic - metabolism</topic><topic>Secretion. Exocytosis</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jones, S.V.Penelope</creatorcontrib><creatorcontrib>Choi, Oksoon H.</creatorcontrib><creatorcontrib>Beaven, Michael A.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jones, S.V.Penelope</au><au>Choi, Oksoon H.</au><au>Beaven, Michael A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carbachol induces secretion in a mast cell line (RBL-2H3) transfected with the ml muscarinic receptor gene</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1991-09-02</date><risdate>1991</risdate><volume>289</volume><issue>1</issue><spage>47</spage><epage>50</epage><pages>47-50</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>The possibility that the ml muscarinic receptor subtype can induce release of intracellular granules and transmitters was studied by transfecting a cultured mast cell line, RBL-2H3 cells, with the ml receptor gene. Comparisons were made between carbachol- and antigen-induced activation of various secretory responses. Like antigen, carbachol stimulated inositol phospholipid hydrolysis and release of arachidonic acid with concomitant dose-dependent secretion of granular contents. Carbachol also stimulated a biphasic increase in intracellular calcium, as measured by single cell fura-2 measurements. Although the kinetics of the carbachol-induced rise in intracellular calcium differed from that induced by antigen, they both utilized the same intracellular pool of calcium, and the second phase of the rise in intracellular calcium was dependent on extracellular calcium in both cases. Thus, the ml muscarinic receptor activates release of granules by a mechanism ostensibly similar to that of antigen.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1832647</pmid><doi>10.1016/0014-5793(91)80905-I</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1991-09, Vol.289 (1), p.47-50 |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | [Ca2+]i acetylcholine Aminoquinolines Animals Antigens - pharmacology Arachidonic Acid Arachidonic Acids - metabolism beta-N-Acetylhexosaminidases - metabolism Biological and medical sciences Calcium - metabolism carbachol Carbachol - pharmacology Cell Line Cell physiology Cloned ml muscarinic receptor concentration of free cytosolic Ca2 dinitrophenylated bovine serum albumin DNP-BSA EGTA ethylene glycol-bis-(β aminoethylether) N,N,N′N′-tetraacetic acid Exocytosis Expression (RBL-2H3 cell) Fluorescent Dyes Fundamental and applied biological sciences. Psychology Hydrolysis IgE immunoglobulin E Inositol Phosphates - metabolism Intracellular signal Mast Cells - metabolism Molecular and cellular biology Phospholipids - metabolism Rats Receptors, Muscarinic - genetics Receptors, Muscarinic - metabolism Secretion. Exocytosis Transfection |
| title | Carbachol induces secretion in a mast cell line (RBL-2H3) transfected with the ml muscarinic receptor gene |
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