Nucleotide sequence of raspberry bushy dwarf virus RNA-2: a bicistronic component of a bipartite genome
Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, U.K. Northern blot analysis with cDNA probes to RNA-3 (1 kb) of raspberry bushy dwarf virus (RBDV) revealed extensive sequence homology with RBDV RNA-2 (2.2 kb). Nucleotide sequencing showed that RNA-2 contains two large open reading fra...
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| Veröffentlicht in: | Journal of general virology 1991-09, Vol.72 (9), p.2183-2189 |
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| creator | Natsuaki, T Mayo, M. A Jolly, C. A Murant, A. F |
| description | Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, U.K.
Northern blot analysis with cDNA probes to RNA-3 (1 kb) of raspberry bushy dwarf virus (RBDV) revealed extensive sequence homology with RBDV RNA-2 (2.2 kb). Nucleotide sequencing showed that RNA-2 contains two large open reading frames (ORFs), of 1074 (5' ORF) and 822 (3' ORF) bases. The 3' ORF is virtually identical in sequence to RNA-3, which encodes the M r 30509 (30K) coat protein. The 5' ORF encodes an M r 38860 (39K) protein which slightly resembles the 32K protein encoded by RNA-3 of alfalfa mosaic virus (AIMV). RBDV RNA-2 resembles AIMV RNA-3 in being bicistronic and encoding a coat protein at the 3' end. Comparing RNA-1 and RNA-2 of RBDV, only the 18 3'-terminal nucleotides are identical in sequence but the 3'-terminal 71 nucleotides of each RNA species have the potential to form similar stem-loop structures.
Present address: Faculty of Agriculture, Utsunomiya University, Mine-machi, Utsunomiya, Tochigi 321, Japan.
Received 19 March 1991;
accepted 23 May 1991. |
| doi_str_mv | 10.1099/0022-1317-72-9-2183 |
| format | Article |
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Northern blot analysis with cDNA probes to RNA-3 (1 kb) of raspberry bushy dwarf virus (RBDV) revealed extensive sequence homology with RBDV RNA-2 (2.2 kb). Nucleotide sequencing showed that RNA-2 contains two large open reading frames (ORFs), of 1074 (5' ORF) and 822 (3' ORF) bases. The 3' ORF is virtually identical in sequence to RNA-3, which encodes the M r 30509 (30K) coat protein. The 5' ORF encodes an M r 38860 (39K) protein which slightly resembles the 32K protein encoded by RNA-3 of alfalfa mosaic virus (AIMV). RBDV RNA-2 resembles AIMV RNA-3 in being bicistronic and encoding a coat protein at the 3' end. Comparing RNA-1 and RNA-2 of RBDV, only the 18 3'-terminal nucleotides are identical in sequence but the 3'-terminal 71 nucleotides of each RNA species have the potential to form similar stem-loop structures.
Present address: Faculty of Agriculture, Utsunomiya University, Mine-machi, Utsunomiya, Tochigi 321, Japan.
Received 19 March 1991;
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Northern blot analysis with cDNA probes to RNA-3 (1 kb) of raspberry bushy dwarf virus (RBDV) revealed extensive sequence homology with RBDV RNA-2 (2.2 kb). Nucleotide sequencing showed that RNA-2 contains two large open reading frames (ORFs), of 1074 (5' ORF) and 822 (3' ORF) bases. The 3' ORF is virtually identical in sequence to RNA-3, which encodes the M r 30509 (30K) coat protein. The 5' ORF encodes an M r 38860 (39K) protein which slightly resembles the 32K protein encoded by RNA-3 of alfalfa mosaic virus (AIMV). RBDV RNA-2 resembles AIMV RNA-3 in being bicistronic and encoding a coat protein at the 3' end. Comparing RNA-1 and RNA-2 of RBDV, only the 18 3'-terminal nucleotides are identical in sequence but the 3'-terminal 71 nucleotides of each RNA species have the potential to form similar stem-loop structures.
Present address: Faculty of Agriculture, Utsunomiya University, Mine-machi, Utsunomiya, Tochigi 321, Japan.
Received 19 March 1991;
accepted 23 May 1991.</description><subject>alfalfa mosaic virus</subject><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Cloning, Molecular</subject><subject>DNA, Viral - genetics</subject><subject>Fruit</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Open Reading Frames</subject><subject>Plant Viruses - genetics</subject><subject>Protein Biosynthesis</subject><subject>RNA, Viral - chemistry</subject><subject>RNA, Viral - genetics</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctq3DAUhkVJSSeTPkEpaBFKNkol2bKs7obQXCCkUJq1kOWjGRXbciU7Yd6-MjMky6y0-C865zsIfWH0ilGlvlPKOWEFk0RyoghndfEBrVhZCcKzfoJWr45P6Cylv5SyshTyFJ2yWgkqxAptH2fbQZh8CzjBvxkGCzg4HE0aG4hxj5s57fa4fTHR4Wcf54R_P24I_4ENbrz1aYph8Bbb0I9hgGFa0os0mjj5CfAWhtDDOfroTJfg8_Fdo6ebn3-u78jDr9v7680DsYKWEzHWlY1gtGVKciXBclNaZqUDV8naNi1AaXnNW15RYE5J5Rx1ylSyoVIVvFijb4feMYa8TZp075OFrjMDhDlpyVmGQqt3jazKfETuXKPiYLQxpBTB6TH63sS9ZlQvd9ALZb1Qzu1a6eUOOfX1WD83PbRvmQP4rF8cdZOs6Vw0Q2b5ahOiLiq5THl5sO38dvfiI-iMs_d5lMYHnc_x9uN_0XOemQ</recordid><startdate>19910901</startdate><enddate>19910901</enddate><creator>Natsuaki, T</creator><creator>Mayo, M. A</creator><creator>Jolly, C. A</creator><creator>Murant, A. F</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19910901</creationdate><title>Nucleotide sequence of raspberry bushy dwarf virus RNA-2: a bicistronic component of a bipartite genome</title><author>Natsuaki, T ; Mayo, M. A ; Jolly, C. A ; Murant, A. F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-acf4b510d197297ec2a4c1c7fef678cbdee4c282d260e1f979ff0f9a67b079323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>alfalfa mosaic virus</topic><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Cloning, Molecular</topic><topic>DNA, Viral - genetics</topic><topic>Fruit</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Open Reading Frames</topic><topic>Plant Viruses - genetics</topic><topic>Protein Biosynthesis</topic><topic>RNA, Viral - chemistry</topic><topic>RNA, Viral - genetics</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Natsuaki, T</creatorcontrib><creatorcontrib>Mayo, M. A</creatorcontrib><creatorcontrib>Jolly, C. A</creatorcontrib><creatorcontrib>Murant, A. F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Natsuaki, T</au><au>Mayo, M. A</au><au>Jolly, C. A</au><au>Murant, A. F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nucleotide sequence of raspberry bushy dwarf virus RNA-2: a bicistronic component of a bipartite genome</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1991-09-01</date><risdate>1991</risdate><volume>72</volume><issue>9</issue><spage>2183</spage><epage>2189</epage><pages>2183-2189</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, U.K.
Northern blot analysis with cDNA probes to RNA-3 (1 kb) of raspberry bushy dwarf virus (RBDV) revealed extensive sequence homology with RBDV RNA-2 (2.2 kb). Nucleotide sequencing showed that RNA-2 contains two large open reading frames (ORFs), of 1074 (5' ORF) and 822 (3' ORF) bases. The 3' ORF is virtually identical in sequence to RNA-3, which encodes the M r 30509 (30K) coat protein. The 5' ORF encodes an M r 38860 (39K) protein which slightly resembles the 32K protein encoded by RNA-3 of alfalfa mosaic virus (AIMV). RBDV RNA-2 resembles AIMV RNA-3 in being bicistronic and encoding a coat protein at the 3' end. Comparing RNA-1 and RNA-2 of RBDV, only the 18 3'-terminal nucleotides are identical in sequence but the 3'-terminal 71 nucleotides of each RNA species have the potential to form similar stem-loop structures.
Present address: Faculty of Agriculture, Utsunomiya University, Mine-machi, Utsunomiya, Tochigi 321, Japan.
Received 19 March 1991;
accepted 23 May 1991.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>1895055</pmid><doi>10.1099/0022-1317-72-9-2183</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | alfalfa mosaic virus Amino Acid Sequence Base Sequence Biological and medical sciences Blotting, Northern Cloning, Molecular DNA, Viral - genetics Fruit Fundamental and applied biological sciences. Psychology Genetics Microbiology Molecular Sequence Data Nucleic Acid Conformation Open Reading Frames Plant Viruses - genetics Protein Biosynthesis RNA, Viral - chemistry RNA, Viral - genetics Sequence Homology, Nucleic Acid Virology |
| title | Nucleotide sequence of raspberry bushy dwarf virus RNA-2: a bicistronic component of a bipartite genome |
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