Whole-body X-irradiation Induces Acute and Transient Expression of Heme Oxygenase-1 in Rat Liver
Activation of the stress-inducible heme oxygenase-1 (HO-1) gene by X-irradiation was investigated in rat liver. When male Wistar MS strain rats (8 weeks) received whole-body irradiation of 17.0 Gy, 7 h later the activity of heme oxygenase in the liver was significantly enhanced (2.5 times). The leve...
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| Veröffentlicht in: | JOURNAL OF RADIATION RESEARCH 2002-06, Vol.43 (2), p.205-210 |
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| creator | Suzuki, Keiko Mori, Masahiko Kugawa, Fumihiko Ishihara, Hiroshi |
| description | Activation of the stress-inducible heme oxygenase-1 (HO-1) gene by X-irradiation was investigated in rat liver. When male Wistar MS strain rats (8 weeks) received whole-body irradiation of 17.0 Gy, 7 h later the activity of heme oxygenase in the liver was significantly enhanced (2.5 times). The level of HO-1 mRNA expression was increased by 2.3 and 4.0 times 2 and 4 h after radiation, and then declined at 7 and 10 h to the level of 2.0 and 1.6 times of the control. When the X-ray dose was varied from 4.0 to 21.7 Gy, the transcription of the gene was enhanced at all doses and the level of activation was dose-dependent. Finally, western blotting of irradiated liver demonstrated a significant increase in the level of HO-1 induced by X-rays, peaking at 4 h. Thus, X-rays were confirmed to be stressors that induce acute HO-1 expression transiently in the liver. |
| doi_str_mv | 10.1269/jrr.43.205 |
| format | Article |
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When male Wistar MS strain rats (8 weeks) received whole-body irradiation of 17.0 Gy, 7 h later the activity of heme oxygenase in the liver was significantly enhanced (2.5 times). The level of HO-1 mRNA expression was increased by 2.3 and 4.0 times 2 and 4 h after radiation, and then declined at 7 and 10 h to the level of 2.0 and 1.6 times of the control. When the X-ray dose was varied from 4.0 to 21.7 Gy, the transcription of the gene was enhanced at all doses and the level of activation was dose-dependent. Finally, western blotting of irradiated liver demonstrated a significant increase in the level of HO-1 induced by X-rays, peaking at 4 h. Thus, X-rays were confirmed to be stressors that induce acute HO-1 expression transiently in the liver.</description><identifier>ISSN: 0449-3060</identifier><identifier>EISSN: 1349-9157</identifier><identifier>DOI: 10.1269/jrr.43.205</identifier><identifier>PMID: 12238335</identifier><language>eng</language><publisher>England: THE JAPAN RADIATION RESEARCH SOCIETY</publisher><subject>Animals ; Heme ; Heme Oxygenase (Decyclizing) - metabolism ; Heme Oxygenase-1 ; Liver ; Liver - enzymology ; Male ; Radiation (Physics) ; Rats ; Rats, Wistar ; Time Factors ; Whole-Body Irradiation</subject><ispartof>JOURNAL OF RADIATION RESEARCH, 2002-06, Vol.43 (2), p.205-210</ispartof><rights>COPYRIGHT 2002 Oxford University Press</rights><rights>Copyright Japan Science and Technology Agency 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c740t-3cea18a310e585b769fb3fe31fbfb99f9464f6f19d812699a2e50f871cd12b6b3</citedby><cites>FETCH-LOGICAL-c740t-3cea18a310e585b769fb3fe31fbfb99f9464f6f19d812699a2e50f871cd12b6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12238335$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suzuki, Keiko</creatorcontrib><creatorcontrib>Mori, Masahiko</creatorcontrib><creatorcontrib>Kugawa, Fumihiko</creatorcontrib><creatorcontrib>Ishihara, Hiroshi</creatorcontrib><creatorcontrib>Redox Regulation Research Group</creatorcontrib><creatorcontrib>National Institute of Radiological Sciences</creatorcontrib><creatorcontrib>College of Pharmacy</creatorcontrib><creatorcontrib>Radiation Hazard Research Group</creatorcontrib><creatorcontrib>Nihon Univ</creatorcontrib><creatorcontrib>Department of Biological Pharmaceutical Sciences</creatorcontrib><title>Whole-body X-irradiation Induces Acute and Transient Expression of Heme Oxygenase-1 in Rat Liver</title><title>JOURNAL OF RADIATION RESEARCH</title><addtitle>J Radiat Res</addtitle><description>Activation of the stress-inducible heme oxygenase-1 (HO-1) gene by X-irradiation was investigated in rat liver. When male Wistar MS strain rats (8 weeks) received whole-body irradiation of 17.0 Gy, 7 h later the activity of heme oxygenase in the liver was significantly enhanced (2.5 times). The level of HO-1 mRNA expression was increased by 2.3 and 4.0 times 2 and 4 h after radiation, and then declined at 7 and 10 h to the level of 2.0 and 1.6 times of the control. When the X-ray dose was varied from 4.0 to 21.7 Gy, the transcription of the gene was enhanced at all doses and the level of activation was dose-dependent. Finally, western blotting of irradiated liver demonstrated a significant increase in the level of HO-1 induced by X-rays, peaking at 4 h. Thus, X-rays were confirmed to be stressors that induce acute HO-1 expression transiently in the liver.</description><subject>Animals</subject><subject>Heme</subject><subject>Heme Oxygenase (Decyclizing) - metabolism</subject><subject>Heme Oxygenase-1</subject><subject>Liver</subject><subject>Liver - enzymology</subject><subject>Male</subject><subject>Radiation (Physics)</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Time Factors</subject><subject>Whole-Body Irradiation</subject><issn>0449-3060</issn><issn>1349-9157</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVFrHCEQx6W0NEeal36AIhT6UNirs67u-niEpAkcBEJK-2Zdd0y97ulVd0vu29fjDgJ9CYIz6G_-zt8h5D2wJdRSfdmktGz4smbiFVkAb1SlQLSvyYI1JedMsjNykbPvGQjJWAfwlpxBXfOOc7EgP7__iiNWfRz29EflUzKDN5OPgd6GYbaY6crOE1ITBvqQTMgew0SvnnYJi2jBoqM3uEV697R_xGAyVkB9oPdmomv_F9M78saZMePFKZ6Tb9dXD5c31fru6-3lal3ZtmFTxS0a6AwHhqITfSuV67lDDq53vVJONbJx0oEauoNtZWoUzHUt2AHqXvb8nHw66u5S_DNjnvTWZ4vjaALGOeu2ZqrjonsRhE4yxZQo4Mf_wE2cUygmNDTlczlval6o5ZF6NCNqH1yckrFlDbj1NgZ0vpyvBJcgRC2gFHw-FtgUc07o9C75rUl7DUwfvOkyUt1wXUZa4A-nHuZ-i8MzehpgAa6PQLn11owxjD7gc6f2t9zElLCosVoz1vBDKC-xIl82YFy1xTH_B21xsEE</recordid><startdate>20020601</startdate><enddate>20020601</enddate><creator>Suzuki, Keiko</creator><creator>Mori, Masahiko</creator><creator>Kugawa, Fumihiko</creator><creator>Ishihara, Hiroshi</creator><general>THE JAPAN RADIATION RESEARCH SOCIETY</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20020601</creationdate><title>Whole-body X-irradiation Induces Acute and Transient Expression of Heme Oxygenase-1 in Rat Liver</title><author>Suzuki, Keiko ; Mori, Masahiko ; Kugawa, Fumihiko ; Ishihara, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c740t-3cea18a310e585b769fb3fe31fbfb99f9464f6f19d812699a2e50f871cd12b6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Heme</topic><topic>Heme Oxygenase (Decyclizing) - metabolism</topic><topic>Heme Oxygenase-1</topic><topic>Liver</topic><topic>Liver - enzymology</topic><topic>Male</topic><topic>Radiation (Physics)</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Time Factors</topic><topic>Whole-Body Irradiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suzuki, Keiko</creatorcontrib><creatorcontrib>Mori, Masahiko</creatorcontrib><creatorcontrib>Kugawa, Fumihiko</creatorcontrib><creatorcontrib>Ishihara, Hiroshi</creatorcontrib><creatorcontrib>Redox Regulation Research Group</creatorcontrib><creatorcontrib>National Institute of Radiological Sciences</creatorcontrib><creatorcontrib>College of Pharmacy</creatorcontrib><creatorcontrib>Radiation Hazard Research Group</creatorcontrib><creatorcontrib>Nihon Univ</creatorcontrib><creatorcontrib>Department of Biological Pharmaceutical Sciences</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>JOURNAL OF RADIATION RESEARCH</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suzuki, Keiko</au><au>Mori, Masahiko</au><au>Kugawa, Fumihiko</au><au>Ishihara, Hiroshi</au><aucorp>Redox Regulation Research Group</aucorp><aucorp>National Institute of Radiological Sciences</aucorp><aucorp>College of Pharmacy</aucorp><aucorp>Radiation Hazard Research Group</aucorp><aucorp>Nihon Univ</aucorp><aucorp>Department of Biological Pharmaceutical Sciences</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Whole-body X-irradiation Induces Acute and Transient Expression of Heme Oxygenase-1 in Rat Liver</atitle><jtitle>JOURNAL OF RADIATION RESEARCH</jtitle><addtitle>J Radiat Res</addtitle><date>2002-06-01</date><risdate>2002</risdate><volume>43</volume><issue>2</issue><spage>205</spage><epage>210</epage><pages>205-210</pages><issn>0449-3060</issn><eissn>1349-9157</eissn><abstract>Activation of the stress-inducible heme oxygenase-1 (HO-1) gene by X-irradiation was investigated in rat liver. When male Wistar MS strain rats (8 weeks) received whole-body irradiation of 17.0 Gy, 7 h later the activity of heme oxygenase in the liver was significantly enhanced (2.5 times). The level of HO-1 mRNA expression was increased by 2.3 and 4.0 times 2 and 4 h after radiation, and then declined at 7 and 10 h to the level of 2.0 and 1.6 times of the control. When the X-ray dose was varied from 4.0 to 21.7 Gy, the transcription of the gene was enhanced at all doses and the level of activation was dose-dependent. Finally, western blotting of irradiated liver demonstrated a significant increase in the level of HO-1 induced by X-rays, peaking at 4 h. Thus, X-rays were confirmed to be stressors that induce acute HO-1 expression transiently in the liver.</abstract><cop>England</cop><pub>THE JAPAN RADIATION RESEARCH SOCIETY</pub><pmid>12238335</pmid><doi>10.1269/jrr.43.205</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Heme Heme Oxygenase (Decyclizing) - metabolism Heme Oxygenase-1 Liver Liver - enzymology Male Radiation (Physics) Rats Rats, Wistar Time Factors Whole-Body Irradiation |
| title | Whole-body X-irradiation Induces Acute and Transient Expression of Heme Oxygenase-1 in Rat Liver |
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