Identification and characterization of incomplete hepatitis A virus particles
1 Institute of Medical Microbiology, University of Bern and 2 Division of Experimental Microbiology, Institute of Clinical Microbiology and Immunology, Frohbergstrasse 3, 9000 St Gallen, Switzerland The range of hepatitis A virus (HAV) particles generated during persistent infection of different cel...
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| Veröffentlicht in: | Journal of general virology 1991-09, Vol.72 (9), p.2159-2166 |
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| container_title | Journal of general virology |
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| creator | Ruchti, Franziska Siegl, Gunter Weitz, Manfred |
| description | 1 Institute of Medical Microbiology, University of Bern
and 2 Division of Experimental Microbiology, Institute of Clinical Microbiology and Immunology, Frohbergstrasse 3, 9000 St Gallen, Switzerland
The range of hepatitis A virus (HAV) particles generated during persistent infection of different cell lines was studied. Buoyant density and sedimentation analyses of cell extracts revealed a uniform profile of particles in all cell lines analysed except for BS-C-1 cells. The virion itself usually represented less than 50% of the total mass of virus antigen. A major portion of the antigen was associated with non-infectious, empty particles, which banded at 1.305 g/ml and 1.20 g/ml CsCl, and sedimented in sucrose gradients at 76S and 59S. Empty HAV particles were similar to those of poliovirus with respect to their physical stability and had the characteristic capsid protein content (VP0, VP1 and VP3). An additional RNA-containing particle, probably the provirion, represented only a minor species characterized by a buoyant density of 1.32 g/ml in CsCl and sedimenting at 130S.
Received 13 February 1991;
accepted 29 May 1991. |
| doi_str_mv | 10.1099/0022-1317-72-9-2159 |
| format | Article |
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and 2 Division of Experimental Microbiology, Institute of Clinical Microbiology and Immunology, Frohbergstrasse 3, 9000 St Gallen, Switzerland
The range of hepatitis A virus (HAV) particles generated during persistent infection of different cell lines was studied. Buoyant density and sedimentation analyses of cell extracts revealed a uniform profile of particles in all cell lines analysed except for BS-C-1 cells. The virion itself usually represented less than 50% of the total mass of virus antigen. A major portion of the antigen was associated with non-infectious, empty particles, which banded at 1.305 g/ml and 1.20 g/ml CsCl, and sedimented in sucrose gradients at 76S and 59S. Empty HAV particles were similar to those of poliovirus with respect to their physical stability and had the characteristic capsid protein content (VP0, VP1 and VP3). An additional RNA-containing particle, probably the provirion, represented only a minor species characterized by a buoyant density of 1.32 g/ml in CsCl and sedimenting at 130S.
Received 13 February 1991;
accepted 29 May 1991.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-72-9-2159</identifier><identifier>PMID: 1654375</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Animals ; Antigens, Viral - chemistry ; Biological and medical sciences ; Blotting, Western ; Capsid - chemistry ; Cell Line ; Centrifugation, Density Gradient ; Centrifugation, Isopycnic ; Fundamental and applied biological sciences. Psychology ; Hepatitis A Antigens ; Hepatovirus - genetics ; Hepatovirus - immunology ; Hepatovirus - physiology ; Humans ; Microbiology ; Morphology, structure, chemical composition, physicochemical properties ; Proviruses - genetics ; Proviruses - immunology ; Proviruses - physiology ; RNA, Viral - analysis ; Vero Cells ; Virion - genetics ; Virion - immunology ; Virion - physiology ; Virology</subject><ispartof>Journal of general virology, 1991-09, Vol.72 (9), p.2159-2166</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-feabcfa307a3893f77c76c055562cf6c94ef1443c89149cedd5929ea1b47c423</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3737,3738,27915,27916</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5215329$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1654375$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ruchti, Franziska</creatorcontrib><creatorcontrib>Siegl, Gunter</creatorcontrib><creatorcontrib>Weitz, Manfred</creatorcontrib><title>Identification and characterization of incomplete hepatitis A virus particles</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>1 Institute of Medical Microbiology, University of Bern
and 2 Division of Experimental Microbiology, Institute of Clinical Microbiology and Immunology, Frohbergstrasse 3, 9000 St Gallen, Switzerland
The range of hepatitis A virus (HAV) particles generated during persistent infection of different cell lines was studied. Buoyant density and sedimentation analyses of cell extracts revealed a uniform profile of particles in all cell lines analysed except for BS-C-1 cells. The virion itself usually represented less than 50% of the total mass of virus antigen. A major portion of the antigen was associated with non-infectious, empty particles, which banded at 1.305 g/ml and 1.20 g/ml CsCl, and sedimented in sucrose gradients at 76S and 59S. Empty HAV particles were similar to those of poliovirus with respect to their physical stability and had the characteristic capsid protein content (VP0, VP1 and VP3). An additional RNA-containing particle, probably the provirion, represented only a minor species characterized by a buoyant density of 1.32 g/ml in CsCl and sedimenting at 130S.
Received 13 February 1991;
accepted 29 May 1991.</description><subject>Animals</subject><subject>Antigens, Viral - chemistry</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Capsid - chemistry</subject><subject>Cell Line</subject><subject>Centrifugation, Density Gradient</subject><subject>Centrifugation, Isopycnic</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hepatitis A Antigens</subject><subject>Hepatovirus - genetics</subject><subject>Hepatovirus - immunology</subject><subject>Hepatovirus - physiology</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Morphology, structure, chemical composition, physicochemical properties</subject><subject>Proviruses - genetics</subject><subject>Proviruses - immunology</subject><subject>Proviruses - physiology</subject><subject>RNA, Viral - analysis</subject><subject>Vero Cells</subject><subject>Virion - genetics</subject><subject>Virion - immunology</subject><subject>Virion - physiology</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLLDEQRoMoOlf9BSL0QuRuWvPsTJYiehUUN-5DprriRPpl0uNFf71petClq0DVqS9Vh5ATRi8YNeaSUs5LJpguNS9NyZkyO2TBZKVKnvu7ZPFNHJA_Kb1SyqRUep_ss0pJodWCPN7X2I3BB3Bj6LvCdXUBaxcdjBjD51zsfRE66NuhwRGLNQ65PIZUXBXvIW5SMbg4BmgwHZE975qEx9v3kDzf3jxf35UPT__ur68eSpBiOZYe3Qq8E1Q7sTTCaw26AqqUqjj4CoxEnzcVsDRMGsC6VoYbdGwlNUguDsn5HDvE_m2DabRtSIBN4zrsN8nqfL4WvPoVZBVVvOIsg2IGIfYpRfR2iKF18cMyaifZdlJpJ5U53Ro7yc5Tp9v4zarF-mdmtpv7Z9u-S-AaH10HIX1jKocIPsX8nbF1eFn_DxHtC3ZtyKusQm-z4p8fvwCCH5V_</recordid><startdate>19910901</startdate><enddate>19910901</enddate><creator>Ruchti, Franziska</creator><creator>Siegl, Gunter</creator><creator>Weitz, Manfred</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19910901</creationdate><title>Identification and characterization of incomplete hepatitis A virus particles</title><author>Ruchti, Franziska ; Siegl, Gunter ; Weitz, Manfred</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-feabcfa307a3893f77c76c055562cf6c94ef1443c89149cedd5929ea1b47c423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Antigens, Viral - chemistry</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Capsid - chemistry</topic><topic>Cell Line</topic><topic>Centrifugation, Density Gradient</topic><topic>Centrifugation, Isopycnic</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hepatitis A Antigens</topic><topic>Hepatovirus - genetics</topic><topic>Hepatovirus - immunology</topic><topic>Hepatovirus - physiology</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Morphology, structure, chemical composition, physicochemical properties</topic><topic>Proviruses - genetics</topic><topic>Proviruses - immunology</topic><topic>Proviruses - physiology</topic><topic>RNA, Viral - analysis</topic><topic>Vero Cells</topic><topic>Virion - genetics</topic><topic>Virion - immunology</topic><topic>Virion - physiology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ruchti, Franziska</creatorcontrib><creatorcontrib>Siegl, Gunter</creatorcontrib><creatorcontrib>Weitz, Manfred</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ruchti, Franziska</au><au>Siegl, Gunter</au><au>Weitz, Manfred</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of incomplete hepatitis A virus particles</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1991-09-01</date><risdate>1991</risdate><volume>72</volume><issue>9</issue><spage>2159</spage><epage>2166</epage><pages>2159-2166</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>1 Institute of Medical Microbiology, University of Bern
and 2 Division of Experimental Microbiology, Institute of Clinical Microbiology and Immunology, Frohbergstrasse 3, 9000 St Gallen, Switzerland
The range of hepatitis A virus (HAV) particles generated during persistent infection of different cell lines was studied. Buoyant density and sedimentation analyses of cell extracts revealed a uniform profile of particles in all cell lines analysed except for BS-C-1 cells. The virion itself usually represented less than 50% of the total mass of virus antigen. A major portion of the antigen was associated with non-infectious, empty particles, which banded at 1.305 g/ml and 1.20 g/ml CsCl, and sedimented in sucrose gradients at 76S and 59S. Empty HAV particles were similar to those of poliovirus with respect to their physical stability and had the characteristic capsid protein content (VP0, VP1 and VP3). An additional RNA-containing particle, probably the provirion, represented only a minor species characterized by a buoyant density of 1.32 g/ml in CsCl and sedimenting at 130S.
Received 13 February 1991;
accepted 29 May 1991.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>1654375</pmid><doi>10.1099/0022-1317-72-9-2159</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; Microbiology Society; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Animals Antigens, Viral - chemistry Biological and medical sciences Blotting, Western Capsid - chemistry Cell Line Centrifugation, Density Gradient Centrifugation, Isopycnic Fundamental and applied biological sciences. Psychology Hepatitis A Antigens Hepatovirus - genetics Hepatovirus - immunology Hepatovirus - physiology Humans Microbiology Morphology, structure, chemical composition, physicochemical properties Proviruses - genetics Proviruses - immunology Proviruses - physiology RNA, Viral - analysis Vero Cells Virion - genetics Virion - immunology Virion - physiology Virology |
| title | Identification and characterization of incomplete hepatitis A virus particles |
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