Cross-reactivity on Western blots in HIV-1 and HIV-2 infections
To examine cross-reactivity of antibodies to heterologous antigens, on HIV-1 and HIV-2 Western blots, we tested sera from 1362 consecutive tuberculosis (TB) patients and 2127 consecutive blood donors. Specimens positive on enzyme-linked immunosorbent assay (ELISA) for HIV-1 or HIV-2 were further cha...
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| Veröffentlicht in: | AIDS (London) 1991-07, Vol.5 (7), p.859-863 |
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| container_title | AIDS (London) |
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| creator | DE COCK, K. M PORTER, A KOUADIO, J MARAN, M LAFONTAINE, M.-F GERSHY-DAMET, G.-M HEYWARD, W. L GEORGE, R |
| description | To examine cross-reactivity of antibodies to heterologous antigens, on HIV-1 and HIV-2 Western blots, we tested sera from 1362 consecutive tuberculosis (TB) patients and 2127 consecutive blood donors. Specimens positive on enzyme-linked immunosorbent assay (ELISA) for HIV-1 or HIV-2 were further characterized by synthetic peptide-based tests, and tested by HIV-1- and HIV-2-specific Western blots. Dual serologic reactivity on synthetic peptide tests was proportionately more frequent in HIV-positive TB patients than in blood donors, and HIV-2 reactivity less frequent. Positive HIV-1 Western blots were seen in 73-83% of specimens specifically characterized as positive for HIV-2 on synthetic peptide tests. Cross-reactivity to HIV-2 Western blots by HIV-1-positive specimens was significantly more frequent in TB patients (35%) than in asymptomatic donors (9%; P less than 0.001). Using recently recommended criteria for HIV-2 Western blot interpretation (presence of two env bands) reduced the overall proportion of HIV-1-positive specimens having a positive HIV-2 Western blot from 27.5 to 16.4%, with [corrected] minimal effect on sensitivity in the diagnosis of HIV-2 reactivity on specimens positive on synthetic peptide tests. |
| doi_str_mv | 10.1097/00002030-199107000-00010 |
| format | Article |
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Cross-reactivity to HIV-2 Western blots by HIV-1-positive specimens was significantly more frequent in TB patients (35%) than in asymptomatic donors (9%; P less than 0.001). Using recently recommended criteria for HIV-2 Western blot interpretation (presence of two env bands) reduced the overall proportion of HIV-1-positive specimens having a positive HIV-2 Western blot from 27.5 to 16.4%, with [corrected] minimal effect on sensitivity in the diagnosis of HIV-2 reactivity on specimens positive on synthetic peptide tests.</description><identifier>ISSN: 0269-9370</identifier><identifier>EISSN: 1473-5571</identifier><identifier>DOI: 10.1097/00002030-199107000-00010</identifier><identifier>PMID: 1892591</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>AIDS/HIV ; Biological and medical sciences ; Blotting, Western ; Cross Reactions ; Enzyme-Linked Immunosorbent Assay ; HIV Antibodies - blood ; HIV Antibodies - immunology ; HIV Infections - complications ; HIV Infections - diagnosis ; HIV Infections - immunology ; HIV-1 - immunology ; HIV-2 - immunology ; human immunodeficiency virus 1 ; human immunodeficiency virus 2 ; Humans ; Immunodeficiencies ; Immunodeficiencies. Immunoglobulinopathies ; Immunopathology ; Medical sciences ; Population ; Tuberculosis - complications ; Tuberculosis - immunology</subject><ispartof>AIDS (London), 1991-07, Vol.5 (7), p.859-863</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-5f70222014a905cedcc85d1dfb7cf565a259ffa981019d2e10332580f300e9733</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19796730$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1892591$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DE COCK, K. M</creatorcontrib><creatorcontrib>PORTER, A</creatorcontrib><creatorcontrib>KOUADIO, J</creatorcontrib><creatorcontrib>MARAN, M</creatorcontrib><creatorcontrib>LAFONTAINE, M.-F</creatorcontrib><creatorcontrib>GERSHY-DAMET, G.-M</creatorcontrib><creatorcontrib>HEYWARD, W. L</creatorcontrib><creatorcontrib>GEORGE, R</creatorcontrib><title>Cross-reactivity on Western blots in HIV-1 and HIV-2 infections</title><title>AIDS (London)</title><addtitle>AIDS</addtitle><description>To examine cross-reactivity of antibodies to heterologous antigens, on HIV-1 and HIV-2 Western blots, we tested sera from 1362 consecutive tuberculosis (TB) patients and 2127 consecutive blood donors. Specimens positive on enzyme-linked immunosorbent assay (ELISA) for HIV-1 or HIV-2 were further characterized by synthetic peptide-based tests, and tested by HIV-1- and HIV-2-specific Western blots. Dual serologic reactivity on synthetic peptide tests was proportionately more frequent in HIV-positive TB patients than in blood donors, and HIV-2 reactivity less frequent. Positive HIV-1 Western blots were seen in 73-83% of specimens specifically characterized as positive for HIV-2 on synthetic peptide tests. Cross-reactivity to HIV-2 Western blots by HIV-1-positive specimens was significantly more frequent in TB patients (35%) than in asymptomatic donors (9%; P less than 0.001). Using recently recommended criteria for HIV-2 Western blot interpretation (presence of two env bands) reduced the overall proportion of HIV-1-positive specimens having a positive HIV-2 Western blot from 27.5 to 16.4%, with [corrected] minimal effect on sensitivity in the diagnosis of HIV-2 reactivity on specimens positive on synthetic peptide tests.</description><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cross Reactions</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>HIV Antibodies - blood</subject><subject>HIV Antibodies - immunology</subject><subject>HIV Infections - complications</subject><subject>HIV Infections - diagnosis</subject><subject>HIV Infections - immunology</subject><subject>HIV-1 - immunology</subject><subject>HIV-2 - immunology</subject><subject>human immunodeficiency virus 1</subject><subject>human immunodeficiency virus 2</subject><subject>Humans</subject><subject>Immunodeficiencies</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Medical sciences</subject><subject>Population</subject><subject>Tuberculosis - complications</subject><subject>Tuberculosis - immunology</subject><issn>0269-9370</issn><issn>1473-5571</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLAzEQgIMotVZ_grAXvUVnkmaTnESKj0LBi49jSLMJrGx3a7IV-u9NH9qjgZBM5pvM8BFSINwgaHkLeTHgQFFrBJkjmjfCERniWHIqhMRjMgRWaqq5hFNyltJnRgQoNSADVJoJjUNyN4ldSjR66_r6u-7XRdcWHz71PrbFvOn6VNRt8Tx9p1jYttreWH4KPvNdm87JSbBN8hf7c0TeHh9eJ8909vI0ndzPqOMSeyqCBMYY4NhqEM5XzilRYRXm0gVRCpunCcFqhYC6Yh6BcyYUBA7gteR8RK53_y5j97XK85lFnZxvGtv6bpWMZKD5WOK_IJZc8LEqM6h2oNsYiD6YZawXNq4NgtlINr-SzZ9ks5WcSy_3PVbzha8OhTurOX-1z9vkbBOibV2dDpiWupQc-A8dj4D9</recordid><startdate>19910701</startdate><enddate>19910701</enddate><creator>DE COCK, K. M</creator><creator>PORTER, A</creator><creator>KOUADIO, J</creator><creator>MARAN, M</creator><creator>LAFONTAINE, M.-F</creator><creator>GERSHY-DAMET, G.-M</creator><creator>HEYWARD, W. L</creator><creator>GEORGE, R</creator><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19910701</creationdate><title>Cross-reactivity on Western blots in HIV-1 and HIV-2 infections</title><author>DE COCK, K. M ; PORTER, A ; KOUADIO, J ; MARAN, M ; LAFONTAINE, M.-F ; GERSHY-DAMET, G.-M ; HEYWARD, W. L ; GEORGE, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-5f70222014a905cedcc85d1dfb7cf565a259ffa981019d2e10332580f300e9733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>AIDS/HIV</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cross Reactions</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>HIV Antibodies - blood</topic><topic>HIV Antibodies - immunology</topic><topic>HIV Infections - complications</topic><topic>HIV Infections - diagnosis</topic><topic>HIV Infections - immunology</topic><topic>HIV-1 - immunology</topic><topic>HIV-2 - immunology</topic><topic>human immunodeficiency virus 1</topic><topic>human immunodeficiency virus 2</topic><topic>Humans</topic><topic>Immunodeficiencies</topic><topic>Immunodeficiencies. Immunoglobulinopathies</topic><topic>Immunopathology</topic><topic>Medical sciences</topic><topic>Population</topic><topic>Tuberculosis - complications</topic><topic>Tuberculosis - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DE COCK, K. M</creatorcontrib><creatorcontrib>PORTER, A</creatorcontrib><creatorcontrib>KOUADIO, J</creatorcontrib><creatorcontrib>MARAN, M</creatorcontrib><creatorcontrib>LAFONTAINE, M.-F</creatorcontrib><creatorcontrib>GERSHY-DAMET, G.-M</creatorcontrib><creatorcontrib>HEYWARD, W. L</creatorcontrib><creatorcontrib>GEORGE, R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>AIDS (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DE COCK, K. M</au><au>PORTER, A</au><au>KOUADIO, J</au><au>MARAN, M</au><au>LAFONTAINE, M.-F</au><au>GERSHY-DAMET, G.-M</au><au>HEYWARD, W. L</au><au>GEORGE, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cross-reactivity on Western blots in HIV-1 and HIV-2 infections</atitle><jtitle>AIDS (London)</jtitle><addtitle>AIDS</addtitle><date>1991-07-01</date><risdate>1991</risdate><volume>5</volume><issue>7</issue><spage>859</spage><epage>863</epage><pages>859-863</pages><issn>0269-9370</issn><eissn>1473-5571</eissn><abstract>To examine cross-reactivity of antibodies to heterologous antigens, on HIV-1 and HIV-2 Western blots, we tested sera from 1362 consecutive tuberculosis (TB) patients and 2127 consecutive blood donors. Specimens positive on enzyme-linked immunosorbent assay (ELISA) for HIV-1 or HIV-2 were further characterized by synthetic peptide-based tests, and tested by HIV-1- and HIV-2-specific Western blots. Dual serologic reactivity on synthetic peptide tests was proportionately more frequent in HIV-positive TB patients than in blood donors, and HIV-2 reactivity less frequent. Positive HIV-1 Western blots were seen in 73-83% of specimens specifically characterized as positive for HIV-2 on synthetic peptide tests. Cross-reactivity to HIV-2 Western blots by HIV-1-positive specimens was significantly more frequent in TB patients (35%) than in asymptomatic donors (9%; P less than 0.001). Using recently recommended criteria for HIV-2 Western blot interpretation (presence of two env bands) reduced the overall proportion of HIV-1-positive specimens having a positive HIV-2 Western blot from 27.5 to 16.4%, with [corrected] minimal effect on sensitivity in the diagnosis of HIV-2 reactivity on specimens positive on synthetic peptide tests.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>1892591</pmid><doi>10.1097/00002030-199107000-00010</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0269-9370 |
| ispartof | AIDS (London), 1991-07, Vol.5 (7), p.859-863 |
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| language | eng |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | AIDS/HIV Biological and medical sciences Blotting, Western Cross Reactions Enzyme-Linked Immunosorbent Assay HIV Antibodies - blood HIV Antibodies - immunology HIV Infections - complications HIV Infections - diagnosis HIV Infections - immunology HIV-1 - immunology HIV-2 - immunology human immunodeficiency virus 1 human immunodeficiency virus 2 Humans Immunodeficiencies Immunodeficiencies. Immunoglobulinopathies Immunopathology Medical sciences Population Tuberculosis - complications Tuberculosis - immunology |
| title | Cross-reactivity on Western blots in HIV-1 and HIV-2 infections |
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