Image analysis quantification of peptide-immunoreactive nerves in the skin of patients with Raynaud's phenomenon and systemic sclerosis
Image analysis quantification was used to assess the results of immunocytochemistry for a neuronal marker and neuropeptides in digital skin biopsies from Raynaud's phenomenon (RP) and systemic sclerosis (SS) patients, to verify the possibility of a selective quantitative abnormality of immunore...
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| Veröffentlicht in: | The Journal of pathology 1991-07, Vol.164 (3), p.245-252 |
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| container_title | The Journal of pathology |
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| creator | Terenghi, Giorgio Bunker, Christopher B. Liu, Yu-Fei Springall, David R. Cowen, Tim Dowd, Pauline M. Polak, Julia M. |
| description | Image analysis quantification was used to assess the results of immunocytochemistry for a neuronal marker and neuropeptides in digital skin biopsies from Raynaud's phenomenon (RP) and systemic sclerosis (SS) patients, to verify the possibility of a selective quantitative abnormality of immunoreactive nerves. The field area of specific immunostaining and nerve counts were evaluated on coded specimens, and the data compared by statistical analysis. Nerves immunoreactive for protein gene product 9.5 (PGP), a marker for neuronal elements, were decreased significantly in epidermal and subepidermal layers of digital skin in RP patients (P < 0.0001). This change was paralleled by a decrease of calcitonin gene‐related peptide (CGRP) immunoreactive nerves in the epidermis and around capillaries in the dermal papillae (P=0.005). In the skin of RP patients, these changes were readily demonstrated by image analysis, although they were not always apparent on visual screening. In digital skin of SS patients, there was a generalized and very significant decrease of PGP, CGRP, and VIP immunoreactivities in all areas (P |
| doi_str_mv | 10.1002/path.1711640310 |
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The field area of specific immunostaining and nerve counts were evaluated on coded specimens, and the data compared by statistical analysis. Nerves immunoreactive for protein gene product 9.5 (PGP), a marker for neuronal elements, were decreased significantly in epidermal and subepidermal layers of digital skin in RP patients (P < 0.0001). This change was paralleled by a decrease of calcitonin gene‐related peptide (CGRP) immunoreactive nerves in the epidermis and around capillaries in the dermal papillae (P=0.005). In the skin of RP patients, these changes were readily demonstrated by image analysis, although they were not always apparent on visual screening. In digital skin of SS patients, there was a generalized and very significant decrease of PGP, CGRP, and VIP immunoreactivities in all areas (P<0.0001). These results demonstrate that neuropeptide‐containing nerves are involved in the digital pathology of RP and SS, and that image analysis quantification is an accurate and sensitive method for assessing morphological changes in pathological samples.</description><identifier>ISSN: 0022-3417</identifier><identifier>EISSN: 1096-9896</identifier><identifier>DOI: 10.1002/path.1711640310</identifier><identifier>PMID: 1832448</identifier><identifier>CODEN: JPTLAS</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Biological and medical sciences ; Calcitonin Gene-Related Peptide - analysis ; Female ; Humans ; image analysis ; Image Processing, Computer-Assisted ; Immunohistochemistry ; Male ; Medical sciences ; Middle Aged ; Neuropeptides ; Neuropeptides - analysis ; quantification ; Raynaud Disease - pathology ; Raynaud's phenomenon ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis ; Scleroderma, Systemic - pathology ; skin ; Skin - innervation ; Ubiquitin Thiolesterase ; Vasoactive Intestinal Peptide - analysis</subject><ispartof>The Journal of pathology, 1991-07, Vol.164 (3), p.245-252</ispartof><rights>Copyright © 1991 John Wiley & Sons, Ltd.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4680-c35ef397aa1786eace2328f0d239b0ace1a71de6b0fee9f418774f1a85cbac143</citedby><cites>FETCH-LOGICAL-c4680-c35ef397aa1786eace2328f0d239b0ace1a71de6b0fee9f418774f1a85cbac143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpath.1711640310$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpath.1711640310$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5002482$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1832448$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Terenghi, Giorgio</creatorcontrib><creatorcontrib>Bunker, Christopher B.</creatorcontrib><creatorcontrib>Liu, Yu-Fei</creatorcontrib><creatorcontrib>Springall, David R.</creatorcontrib><creatorcontrib>Cowen, Tim</creatorcontrib><creatorcontrib>Dowd, Pauline M.</creatorcontrib><creatorcontrib>Polak, Julia M.</creatorcontrib><title>Image analysis quantification of peptide-immunoreactive nerves in the skin of patients with Raynaud's phenomenon and systemic sclerosis</title><title>The Journal of pathology</title><addtitle>J. Pathol</addtitle><description>Image analysis quantification was used to assess the results of immunocytochemistry for a neuronal marker and neuropeptides in digital skin biopsies from Raynaud's phenomenon (RP) and systemic sclerosis (SS) patients, to verify the possibility of a selective quantitative abnormality of immunoreactive nerves. The field area of specific immunostaining and nerve counts were evaluated on coded specimens, and the data compared by statistical analysis. Nerves immunoreactive for protein gene product 9.5 (PGP), a marker for neuronal elements, were decreased significantly in epidermal and subepidermal layers of digital skin in RP patients (P < 0.0001). This change was paralleled by a decrease of calcitonin gene‐related peptide (CGRP) immunoreactive nerves in the epidermis and around capillaries in the dermal papillae (P=0.005). In the skin of RP patients, these changes were readily demonstrated by image analysis, although they were not always apparent on visual screening. In digital skin of SS patients, there was a generalized and very significant decrease of PGP, CGRP, and VIP immunoreactivities in all areas (P<0.0001). These results demonstrate that neuropeptide‐containing nerves are involved in the digital pathology of RP and SS, and that image analysis quantification is an accurate and sensitive method for assessing morphological changes in pathological samples.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>Calcitonin Gene-Related Peptide - analysis</subject><subject>Female</subject><subject>Humans</subject><subject>image analysis</subject><subject>Image Processing, Computer-Assisted</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neuropeptides</subject><subject>Neuropeptides - analysis</subject><subject>quantification</subject><subject>Raynaud Disease - pathology</subject><subject>Raynaud's phenomenon</subject><subject>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</subject><subject>Scleroderma, Systemic - pathology</subject><subject>skin</subject><subject>Skin - innervation</subject><subject>Ubiquitin Thiolesterase</subject><subject>Vasoactive Intestinal Peptide - analysis</subject><issn>0022-3417</issn><issn>1096-9896</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EKkvhzAnJB1ROaT2JE8fiVFXQrVQBQovozZp1Jqxp4qRx0pJfwN_GVVatOPVgWdZ8772xHmNvQRyDEOlJj-PuGBRAIUUG4hlbgdBFoktdPGerSKRJJkG9ZK9C-C2E0DrPD9gBlFkqZblify9a_EUcPTZzcIHfTOhHVzuLo-s872reUz-6ihLXtpPvBkI7ulvinoZbCtx5Pu6Ih2u3wFFGfgz8zo07_h1nj1P1IfB-R75r4_ExquJhDiO1zvJgGxq6GPyavaixCfRmfx-yH58_bc7WyeXX84uz08vEyqIUic1yqjOtEEGVRdyF0iwta1Glmd6K-ARUUFGxFTWRriWUSskasMztFi3I7JAdLb790N1MFEbTumCpadBTNwWjUqFBa3gShAJAFXkRwZMFtPEjYaDa9INrcZgNCHPfkbnvyDx2FBXv9tbTtqXqkV9KifP3-zkGi009oLcuPGB59JRlGrGPC3bnGpqfSjXfTjfr_5ZIFrWLVfx5UONwbQqVqdz8_HJu1lJcyXyjzVX2Dyesvfc</recordid><startdate>199107</startdate><enddate>199107</enddate><creator>Terenghi, Giorgio</creator><creator>Bunker, Christopher B.</creator><creator>Liu, Yu-Fei</creator><creator>Springall, David R.</creator><creator>Cowen, Tim</creator><creator>Dowd, Pauline M.</creator><creator>Polak, Julia M.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199107</creationdate><title>Image analysis quantification of peptide-immunoreactive nerves in the skin of patients with Raynaud's phenomenon and systemic sclerosis</title><author>Terenghi, Giorgio ; Bunker, Christopher B. ; Liu, Yu-Fei ; Springall, David R. ; Cowen, Tim ; Dowd, Pauline M. ; Polak, Julia M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4680-c35ef397aa1786eace2328f0d239b0ace1a71de6b0fee9f418774f1a85cbac143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Calcitonin Gene-Related Peptide - analysis</topic><topic>Female</topic><topic>Humans</topic><topic>image analysis</topic><topic>Image Processing, Computer-Assisted</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neuropeptides</topic><topic>Neuropeptides - analysis</topic><topic>quantification</topic><topic>Raynaud Disease - pathology</topic><topic>Raynaud's phenomenon</topic><topic>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</topic><topic>Scleroderma, Systemic - pathology</topic><topic>skin</topic><topic>Skin - innervation</topic><topic>Ubiquitin Thiolesterase</topic><topic>Vasoactive Intestinal Peptide - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Terenghi, Giorgio</creatorcontrib><creatorcontrib>Bunker, Christopher B.</creatorcontrib><creatorcontrib>Liu, Yu-Fei</creatorcontrib><creatorcontrib>Springall, David R.</creatorcontrib><creatorcontrib>Cowen, Tim</creatorcontrib><creatorcontrib>Dowd, Pauline M.</creatorcontrib><creatorcontrib>Polak, Julia M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Terenghi, Giorgio</au><au>Bunker, Christopher B.</au><au>Liu, Yu-Fei</au><au>Springall, David R.</au><au>Cowen, Tim</au><au>Dowd, Pauline M.</au><au>Polak, Julia M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Image analysis quantification of peptide-immunoreactive nerves in the skin of patients with Raynaud's phenomenon and systemic sclerosis</atitle><jtitle>The Journal of pathology</jtitle><addtitle>J. Pathol</addtitle><date>1991-07</date><risdate>1991</risdate><volume>164</volume><issue>3</issue><spage>245</spage><epage>252</epage><pages>245-252</pages><issn>0022-3417</issn><eissn>1096-9896</eissn><coden>JPTLAS</coden><abstract>Image analysis quantification was used to assess the results of immunocytochemistry for a neuronal marker and neuropeptides in digital skin biopsies from Raynaud's phenomenon (RP) and systemic sclerosis (SS) patients, to verify the possibility of a selective quantitative abnormality of immunoreactive nerves. The field area of specific immunostaining and nerve counts were evaluated on coded specimens, and the data compared by statistical analysis. Nerves immunoreactive for protein gene product 9.5 (PGP), a marker for neuronal elements, were decreased significantly in epidermal and subepidermal layers of digital skin in RP patients (P < 0.0001). This change was paralleled by a decrease of calcitonin gene‐related peptide (CGRP) immunoreactive nerves in the epidermis and around capillaries in the dermal papillae (P=0.005). In the skin of RP patients, these changes were readily demonstrated by image analysis, although they were not always apparent on visual screening. In digital skin of SS patients, there was a generalized and very significant decrease of PGP, CGRP, and VIP immunoreactivities in all areas (P<0.0001). These results demonstrate that neuropeptide‐containing nerves are involved in the digital pathology of RP and SS, and that image analysis quantification is an accurate and sensitive method for assessing morphological changes in pathological samples.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>1832448</pmid><doi>10.1002/path.1711640310</doi><tpages>8</tpages></addata></record> |
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| ispartof | The Journal of pathology, 1991-07, Vol.164 (3), p.245-252 |
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| subjects | Adult Aged Aged, 80 and over Biological and medical sciences Calcitonin Gene-Related Peptide - analysis Female Humans image analysis Image Processing, Computer-Assisted Immunohistochemistry Male Medical sciences Middle Aged Neuropeptides Neuropeptides - analysis quantification Raynaud Disease - pathology Raynaud's phenomenon Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis Scleroderma, Systemic - pathology skin Skin - innervation Ubiquitin Thiolesterase Vasoactive Intestinal Peptide - analysis |
| title | Image analysis quantification of peptide-immunoreactive nerves in the skin of patients with Raynaud's phenomenon and systemic sclerosis |
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