Problems encountered in detecting a targeted gene by the polymerase chain reaction

We have investigated problems encountered when using the polymerase chain reaction (PCR) to detect recombinants in gene targeting experiments in which homologous recombination occurs between incoming DNA and an endogenous target sequence. The targeting system studied was designed to correct a human...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Gene 1991-07, Vol.103 (2), p.227-233
Hauptverfasser:	Hyung-Suk, Kim, Popovich, Bradley W., Shehee, W.Ronald, Shesely, Edward G., Smithies, Oliver
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Animals Base Sequence Biological and medical sciences Biotechnology Blotting, Southern Cell Nucleus Chromosomes, Human, Pair 11 Electric Stimulation electroporation False Positive Reactions Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetic Therapy - methods Globins - genetics homologous recombination Humans In vitro gene amplification. Pcr technique Methods. Procedures. Technologies Mice Microinjections Molecular Sequence Data Mutation - genetics nuclear microinjection PCR contamination Polymerase Chain Reaction - methods Recombination, Genetic sickle cell mutation Sickle Cell Trait - genetics Tumor Cells, Cultured β-globin gene
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!