Detection of Growth Hormone and Growth Hormone-Releasing Hormone-Related Messenger RNA in Rat Leukocytes by the Polymerase Chain Reaction
Abstract To validate that growth hormone (GH) and growth hormone-releasing hormone (GHRH) can be produced by leukocytes, we have assessed the presence of GH and GHRH-related mRNA in leukocyte cultures by reverse transcription and the polymerase chain reaction. A sample of the polymerase chain reacti...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1991-10, Vol.198 (1), p.643-648 |
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| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 198 |
| creator | Weigent, Douglas A. Riley, John E. Galin, F. Shawn LeBoeuf, Robert D. Blalock, J. Edwin |
| description | Abstract
To validate that growth hormone (GH) and growth hormone-releasing hormone (GHRH) can be produced by leukocytes, we have assessed the presence of GH and GHRH-related mRNA in leukocyte cultures by reverse transcription and the polymerase chain reaction. A sample of the polymerase chain reactions were size-fractionated by electrophoresis in a 0.8% agarose gel and examined with ultraviolet light after ethidium bromide staining. Single major DNA bands corresponding in length to the distance between the 5'ends of the two GH and GHRH specific primers, 603 base pairs and 260 base pairs, respectively, were obtained. The DNA bands hybridized specifically to GH- and GHRH-specific probes after Southern transfer to nitrocellulose. The identity of the GH polymerase chain reaction material was confirmed by restriction enzyme analysis. The results showed that GH and GHRH gene expression occurs in mononuclear leukocytes and support the idea that these neuroendocrine hormones may be common signal molecules between the immune and neuroendocrine systems. [P.S.E.B.M. 1991, Vol 198] |
| doi_str_mv | 10.3181/00379727-198-43302 |
| format | Article |
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To validate that growth hormone (GH) and growth hormone-releasing hormone (GHRH) can be produced by leukocytes, we have assessed the presence of GH and GHRH-related mRNA in leukocyte cultures by reverse transcription and the polymerase chain reaction. A sample of the polymerase chain reactions were size-fractionated by electrophoresis in a 0.8% agarose gel and examined with ultraviolet light after ethidium bromide staining. Single major DNA bands corresponding in length to the distance between the 5'ends of the two GH and GHRH specific primers, 603 base pairs and 260 base pairs, respectively, were obtained. The DNA bands hybridized specifically to GH- and GHRH-specific probes after Southern transfer to nitrocellulose. The identity of the GH polymerase chain reaction material was confirmed by restriction enzyme analysis. The results showed that GH and GHRH gene expression occurs in mononuclear leukocytes and support the idea that these neuroendocrine hormones may be common signal molecules between the immune and neuroendocrine systems. [P.S.E.B.M. 1991, Vol 198]</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>EISSN: 1525-1373</identifier><identifier>DOI: 10.3181/00379727-198-43302</identifier><identifier>PMID: 1679948</identifier><identifier>CODEN: PSEBAA</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Blotting, Southern ; Chromosome Mapping ; Fundamental and applied biological sciences. Psychology ; Growth Hormone - biosynthesis ; Growth Hormone - genetics ; Growth Hormone-Releasing Hormone - biosynthesis ; Growth Hormone-Releasing Hormone - genetics ; Hormones and neuropeptides. Regulation ; Hypothalamus. Hypophysis. Epiphysis. Urophysis ; Leukocytes - metabolism ; Male ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Oligonucleotide Probes - genetics ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Rats ; Rats, Inbred Strains ; RNA, Messenger - analysis ; Vertebrates: endocrinology</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1991-10, Vol.198 (1), p.643-648</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c367t-de22860571c8666b5bcca41e753ecdd641b1194b4126da9b5603699c5dcff3783</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5418157$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1679948$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weigent, Douglas A.</creatorcontrib><creatorcontrib>Riley, John E.</creatorcontrib><creatorcontrib>Galin, F. Shawn</creatorcontrib><creatorcontrib>LeBoeuf, Robert D.</creatorcontrib><creatorcontrib>Blalock, J. Edwin</creatorcontrib><title>Detection of Growth Hormone and Growth Hormone-Releasing Hormone-Related Messenger RNA in Rat Leukocytes by the Polymerase Chain Reaction</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Abstract
To validate that growth hormone (GH) and growth hormone-releasing hormone (GHRH) can be produced by leukocytes, we have assessed the presence of GH and GHRH-related mRNA in leukocyte cultures by reverse transcription and the polymerase chain reaction. A sample of the polymerase chain reactions were size-fractionated by electrophoresis in a 0.8% agarose gel and examined with ultraviolet light after ethidium bromide staining. Single major DNA bands corresponding in length to the distance between the 5'ends of the two GH and GHRH specific primers, 603 base pairs and 260 base pairs, respectively, were obtained. The DNA bands hybridized specifically to GH- and GHRH-specific probes after Southern transfer to nitrocellulose. The identity of the GH polymerase chain reaction material was confirmed by restriction enzyme analysis. The results showed that GH and GHRH gene expression occurs in mononuclear leukocytes and support the idea that these neuroendocrine hormones may be common signal molecules between the immune and neuroendocrine systems. [P.S.E.B.M. 1991, Vol 198]</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Chromosome Mapping</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth Hormone - biosynthesis</subject><subject>Growth Hormone - genetics</subject><subject>Growth Hormone-Releasing Hormone - biosynthesis</subject><subject>Growth Hormone-Releasing Hormone - genetics</subject><subject>Hormones and neuropeptides. Regulation</subject><subject>Hypothalamus. Hypophysis. Epiphysis. Urophysis</subject><subject>Leukocytes - metabolism</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>Oligonucleotide Probes - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>RNA, Messenger - analysis</subject><subject>Vertebrates: endocrinology</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><issn>1525-1373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1u1DAURi0EKkPhBZCQvEDdhfontuNlNS0t0kCrEawtx7mZSZvYre0IzSPw1iSdAaouWF3p3vN9VzoIvafkE6cVPSWEK62YKqiuipJzwl6gBRVcFFxq_RItZqCYidfoTUq3hFChmDxCR1QqrctqgX6dQwaXu-BxaPFlDD_zFl-FOAQP2Prm2apYQw82dX7zdGMzNPgrpAR-AxGvv53hzuO1zXgF411wuwwJ1zuct4BvQr8bINoEeLm1Mwb28f9b9Kq1fYJ3h3mMfny--L68KlbXl1-WZ6vCcaly0QBjlSRCUVdJKWtRO2dLCkpwcE0jS1pTqsu6pEw2VtdCklmGE41rW64qfoxO9r33MTyMkLIZuuSg762HMCajGKm0ZmwC2R50MaQUoTX3sRts3BlKzOzf_PFvJv_m0f8U-nBoH-sBmn-RvfDp_vFwt8nZvo3Wuy79xUQ59Qo1Yad7LNkNmNswRj85-d_j3yO0m8o</recordid><startdate>19911001</startdate><enddate>19911001</enddate><creator>Weigent, Douglas A.</creator><creator>Riley, John E.</creator><creator>Galin, F. Shawn</creator><creator>LeBoeuf, Robert D.</creator><creator>Blalock, J. Edwin</creator><general>SAGE Publications</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19911001</creationdate><title>Detection of Growth Hormone and Growth Hormone-Releasing Hormone-Related Messenger RNA in Rat Leukocytes by the Polymerase Chain Reaction</title><author>Weigent, Douglas A. ; Riley, John E. ; Galin, F. Shawn ; LeBoeuf, Robert D. ; Blalock, J. Edwin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-de22860571c8666b5bcca41e753ecdd641b1194b4126da9b5603699c5dcff3783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Chromosome Mapping</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth Hormone - biosynthesis</topic><topic>Growth Hormone - genetics</topic><topic>Growth Hormone-Releasing Hormone - biosynthesis</topic><topic>Growth Hormone-Releasing Hormone - genetics</topic><topic>Hormones and neuropeptides. Regulation</topic><topic>Hypothalamus. Hypophysis. Epiphysis. Urophysis</topic><topic>Leukocytes - metabolism</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Hybridization</topic><topic>Oligonucleotide Probes - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>RNA, Messenger - analysis</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weigent, Douglas A.</creatorcontrib><creatorcontrib>Riley, John E.</creatorcontrib><creatorcontrib>Galin, F. Shawn</creatorcontrib><creatorcontrib>LeBoeuf, Robert D.</creatorcontrib><creatorcontrib>Blalock, J. 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Edwin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Growth Hormone and Growth Hormone-Releasing Hormone-Related Messenger RNA in Rat Leukocytes by the Polymerase Chain Reaction</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1991-10-01</date><risdate>1991</risdate><volume>198</volume><issue>1</issue><spage>643</spage><epage>648</epage><pages>643-648</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><eissn>1525-1373</eissn><coden>PSEBAA</coden><abstract>Abstract
To validate that growth hormone (GH) and growth hormone-releasing hormone (GHRH) can be produced by leukocytes, we have assessed the presence of GH and GHRH-related mRNA in leukocyte cultures by reverse transcription and the polymerase chain reaction. A sample of the polymerase chain reactions were size-fractionated by electrophoresis in a 0.8% agarose gel and examined with ultraviolet light after ethidium bromide staining. Single major DNA bands corresponding in length to the distance between the 5'ends of the two GH and GHRH specific primers, 603 base pairs and 260 base pairs, respectively, were obtained. The DNA bands hybridized specifically to GH- and GHRH-specific probes after Southern transfer to nitrocellulose. The identity of the GH polymerase chain reaction material was confirmed by restriction enzyme analysis. The results showed that GH and GHRH gene expression occurs in mononuclear leukocytes and support the idea that these neuroendocrine hormones may be common signal molecules between the immune and neuroendocrine systems. [P.S.E.B.M. 1991, Vol 198]</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>1679948</pmid><doi>10.3181/00379727-198-43302</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Base Sequence Biological and medical sciences Blotting, Southern Chromosome Mapping Fundamental and applied biological sciences. Psychology Growth Hormone - biosynthesis Growth Hormone - genetics Growth Hormone-Releasing Hormone - biosynthesis Growth Hormone-Releasing Hormone - genetics Hormones and neuropeptides. Regulation Hypothalamus. Hypophysis. Epiphysis. Urophysis Leukocytes - metabolism Male Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotide Probes - genetics Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Rats Rats, Inbred Strains RNA, Messenger - analysis Vertebrates: endocrinology |
| title | Detection of Growth Hormone and Growth Hormone-Releasing Hormone-Related Messenger RNA in Rat Leukocytes by the Polymerase Chain Reaction |
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