Control of complex formation of DNA polymerase α–primase and cell‐free DNA replication by the C‐terminal amino acids of the largest subunit p180
DNA polymerase α–primase is a heterotetrameric complex essential for simian vacuolating virus 40 (SV40) DNA replication. We show that the C‐terminal 67 amino acid residues of the human p180 subunit are essential for SV40 DNA replication as they are required for binding of the p68 subunit and play a...
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| Veröffentlicht in: | FEBS letters 2002-09, Vol.527 (1-3), p.143-146 |
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| creator | Smith, R.W.P Nasheuer, H.-P |
| description | DNA polymerase α–primase is a heterotetrameric complex essential for simian vacuolating virus 40 (SV40) DNA replication. We show that the C‐terminal 67 amino acid residues of the human p180 subunit are essential for SV40 DNA replication as they are required for binding of the p68 subunit and play a role in the interaction with the primase subunits, p48 and p58. Furthermore, we demonstrate that exchanging these residues to those of mouse origin can only partially rescue the SV40 DNA replication activity of DNA polymerase α–primase. |
| doi_str_mv | 10.1016/S0014-5793(02)03197-6 |
| format | Article |
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We show that the C‐terminal 67 amino acid residues of the human p180 subunit are essential for SV40 DNA replication as they are required for binding of the p68 subunit and play a role in the interaction with the primase subunits, p48 and p58. 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We show that the C‐terminal 67 amino acid residues of the human p180 subunit are essential for SV40 DNA replication as they are required for binding of the p68 subunit and play a role in the interaction with the primase subunits, p48 and p58. Furthermore, we demonstrate that exchanging these residues to those of mouse origin can only partially rescue the SV40 DNA replication activity of DNA polymerase α–primase.</description><subject>Animals</subject><subject>Cell-Free System</subject><subject>DNA Polymerase I - genetics</subject><subject>DNA Polymerase I - metabolism</subject><subject>DNA polymerase α</subject><subject>DNA Primase - genetics</subject><subject>DNA Primase - metabolism</subject><subject>DNA Replication</subject><subject>Enzyme activity</subject><subject>Humans</subject><subject>Mice</subject><subject>Protein Subunits</subject><subject>Protein–protein interaction</subject><subject>Sequence Deletion</subject><subject>Simian vacuolating virus 40 DNA replication</subject><subject>Simian virus 40 - genetics</subject><subject>SV40, simian vacuolating virus 40</subject><subject>TAg, large T antigen</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUUFu1TAQtRCIfgpHAHmF6CLgSZzEWbafliJVsADWluOMIciJg50I_q5HqMSCc3ARDtGTYOd_wRJWo5n33oz9HiGPgT0HBtWLd4wBz8q6KZ6x_IQV0NRZdYdsQNRFVvBK3CWbP5Qj8iCEzyz2Apr75AjyPGdVyTbkx9aNs3eWOkO1GyaL36hxflBz78Y0fPnmlE7O7gb0KiD99fP2-vvk-yE1auyoRmtvr2-MR1y5Hifb67283dH5E9JtxGf0Qz8qS1UsjirddyGtT7hV_iOGmYalXcZ-phMI9pDcM8oGfHSox-TDxfn77WV29fbV6-3pVaZ5dCGrdVWKkgPr6qIVHLlRHdPY1WBEUwK0HNF0DddVw-uSsa4wORjAMikaaItj8nS_d_LuyxJfIYc-pD-pEd0SZJ0zEX1r_kmMvgNwAZFY7onauxA8Grna5XcSmEzRyTU6mXKRLJdrdLKKuieHA0s7YPdXdcgqEi73hK-9xd3_bZUX52f5iiSA5eu4Kn4DVUCsWQ</recordid><startdate>20020911</startdate><enddate>20020911</enddate><creator>Smith, R.W.P</creator><creator>Nasheuer, H.-P</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20020911</creationdate><title>Control of complex formation of DNA polymerase α–primase and cell‐free DNA replication by the C‐terminal amino acids of the largest subunit p180</title><author>Smith, R.W.P ; Nasheuer, H.-P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4016-7c6585410d73b84e4fad0ced71f89511b4eefd94c6947500d3f21f1e510d791b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Cell-Free System</topic><topic>DNA Polymerase I - genetics</topic><topic>DNA Polymerase I - metabolism</topic><topic>DNA polymerase α</topic><topic>DNA Primase - genetics</topic><topic>DNA Primase - metabolism</topic><topic>DNA Replication</topic><topic>Enzyme activity</topic><topic>Humans</topic><topic>Mice</topic><topic>Protein Subunits</topic><topic>Protein–protein interaction</topic><topic>Sequence Deletion</topic><topic>Simian vacuolating virus 40 DNA replication</topic><topic>Simian virus 40 - genetics</topic><topic>SV40, simian vacuolating virus 40</topic><topic>TAg, large T antigen</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smith, R.W.P</creatorcontrib><creatorcontrib>Nasheuer, H.-P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith, R.W.P</au><au>Nasheuer, H.-P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Control of complex formation of DNA polymerase α–primase and cell‐free DNA replication by the C‐terminal amino acids of the largest subunit p180</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2002-09-11</date><risdate>2002</risdate><volume>527</volume><issue>1-3</issue><spage>143</spage><epage>146</epage><pages>143-146</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>DNA polymerase α–primase is a heterotetrameric complex essential for simian vacuolating virus 40 (SV40) DNA replication. We show that the C‐terminal 67 amino acid residues of the human p180 subunit are essential for SV40 DNA replication as they are required for binding of the p68 subunit and play a role in the interaction with the primase subunits, p48 and p58. Furthermore, we demonstrate that exchanging these residues to those of mouse origin can only partially rescue the SV40 DNA replication activity of DNA polymerase α–primase.</abstract><cop>England</cop><pmid>12220650</pmid><doi>10.1016/S0014-5793(02)03197-6</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 2002-09, Vol.527 (1-3), p.143-146 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | Wiley-Blackwell Journals; MEDLINE; Elsevier ScienceDirect Journals Complete; Wiley Online Library Free Content; Free E-Journal (出版社公開部分のみ）; Alma/SFX Local Collection |
| subjects | Animals Cell-Free System DNA Polymerase I - genetics DNA Polymerase I - metabolism DNA polymerase α DNA Primase - genetics DNA Primase - metabolism DNA Replication Enzyme activity Humans Mice Protein Subunits Protein–protein interaction Sequence Deletion Simian vacuolating virus 40 DNA replication Simian virus 40 - genetics SV40, simian vacuolating virus 40 TAg, large T antigen |
| title | Control of complex formation of DNA polymerase α–primase and cell‐free DNA replication by the C‐terminal amino acids of the largest subunit p180 |
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