Effect of group thawing on post-thaw viability of bovine spermatozoa packaged in .5-milliliter French straws
The objective of this study was to determine the effects of thawing groups of 2, 5, 10, 15, or 20 .5-ml French straws on post-thaw spermatozoal viability. Thermostatically controlled and nonthermostatically controlled thawing baths were compared. Using a split-plot design, semen from 10 bulls was ex...
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| Veröffentlicht in: | Journal of animal science 1991-06, Vol.69 (6), p.2303-2309 |
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| creator | Brown, D. W., Jr Senger, P. L Becker, W. C |
| description | The objective of this study was to determine the effects of thawing groups of 2, 5, 10, 15, or 20 .5-ml French straws on post-thaw spermatozoal viability. Thermostatically controlled and nonthermostatically controlled thawing baths were compared. Using a split-plot design, semen from 10 bulls was extended in egg yolk citrate, frozen, and then thawed (in the respective groups) at 36 degrees C in two types of thawing baths. Motility and percentage of intact acrosomes were determined immediately after thawing (0 h) and again after 4 h of incubation at the respective temperature of each thawing bath. Neither percentage of intact acrosomes nor motility was influenced by the number of straws thawed at 0 h (P > .05). Thawing bath had no effect (P > .05) on motility or percentage of intact acrosomes at 0 h. Bull variation was significant in both the 0- and 4-h evaluations. After 4 h of incubation, there was a significant (P < .05) straw number X thawing bath interaction. When 15 or 20 straws were thawed in the thermostatically controlled bath there was a reduction (P < .05) in motility and percentage of intact acrosomes. However, in the nonthermostatically controlled bath there was no reduction in motility and percentage of intact acrosomes as the size of straw group increased. Our results indicate that, when using a nonthermostatically controlled thawing bath, semen packaged in .5-ml straws can be thawed in groups of 20 without an effect on post-thaw sperm viability. |
| doi_str_mv | 10.2527/1991.6962303x |
| format | Article |
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W., Jr ; Senger, P. L ; Becker, W. C</creator><creatorcontrib>Brown, D. W., Jr ; Senger, P. L ; Becker, W. C ; Washington State University, Pullman</creatorcontrib><description>The objective of this study was to determine the effects of thawing groups of 2, 5, 10, 15, or 20 .5-ml French straws on post-thaw spermatozoal viability. Thermostatically controlled and nonthermostatically controlled thawing baths were compared. Using a split-plot design, semen from 10 bulls was extended in egg yolk citrate, frozen, and then thawed (in the respective groups) at 36 degrees C in two types of thawing baths. Motility and percentage of intact acrosomes were determined immediately after thawing (0 h) and again after 4 h of incubation at the respective temperature of each thawing bath. Neither percentage of intact acrosomes nor motility was influenced by the number of straws thawed at 0 h (P > .05). Thawing bath had no effect (P > .05) on motility or percentage of intact acrosomes at 0 h. Bull variation was significant in both the 0- and 4-h evaluations. After 4 h of incubation, there was a significant (P < .05) straw number X thawing bath interaction. When 15 or 20 straws were thawed in the thermostatically controlled bath there was a reduction (P < .05) in motility and percentage of intact acrosomes. However, in the nonthermostatically controlled bath there was no reduction in motility and percentage of intact acrosomes as the size of straw group increased. Our results indicate that, when using a nonthermostatically controlled thawing bath, semen packaged in .5-ml straws can be thawed in groups of 20 without an effect on post-thaw sperm viability.</description><identifier>ISSN: 0021-8812</identifier><identifier>EISSN: 1525-3163</identifier><identifier>EISSN: 0021-8812</identifier><identifier>DOI: 10.2527/1991.6962303x</identifier><identifier>PMID: 1885350</identifier><language>eng</language><publisher>United States: Am Soc Animal Sci</publisher><subject>Analysis of Variance ; Animals ; artificial insemination ; bulls ; Cattle - physiology ; Cell Survival ; congelacion ; congelation ; Cryopreservation - veterinary ; decongelation ; descongelacion ; freezing ; inseminacion artificial ; insemination artificielle ; Male ; mouvement ; movement ; movimiento ; semen ; Semen Preservation - veterinary ; Sperm Motility ; Spermatozoa - physiology ; sperme ; taureau ; Temperature ; thawing ; toro ; viabilidad ; viabilite ; viability</subject><ispartof>Journal of animal science, 1991-06, Vol.69 (6), p.2303-2309</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c257t-bb1163597304d166635ee187b0a3f4029700f176e28ff978599f2b81a605ceaf3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1885350$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brown, D. W., Jr</creatorcontrib><creatorcontrib>Senger, P. L</creatorcontrib><creatorcontrib>Becker, W. C</creatorcontrib><creatorcontrib>Washington State University, Pullman</creatorcontrib><title>Effect of group thawing on post-thaw viability of bovine spermatozoa packaged in .5-milliliter French straws</title><title>Journal of animal science</title><addtitle>J Anim Sci</addtitle><description>The objective of this study was to determine the effects of thawing groups of 2, 5, 10, 15, or 20 .5-ml French straws on post-thaw spermatozoal viability. Thermostatically controlled and nonthermostatically controlled thawing baths were compared. Using a split-plot design, semen from 10 bulls was extended in egg yolk citrate, frozen, and then thawed (in the respective groups) at 36 degrees C in two types of thawing baths. Motility and percentage of intact acrosomes were determined immediately after thawing (0 h) and again after 4 h of incubation at the respective temperature of each thawing bath. Neither percentage of intact acrosomes nor motility was influenced by the number of straws thawed at 0 h (P > .05). Thawing bath had no effect (P > .05) on motility or percentage of intact acrosomes at 0 h. Bull variation was significant in both the 0- and 4-h evaluations. After 4 h of incubation, there was a significant (P < .05) straw number X thawing bath interaction. When 15 or 20 straws were thawed in the thermostatically controlled bath there was a reduction (P < .05) in motility and percentage of intact acrosomes. However, in the nonthermostatically controlled bath there was no reduction in motility and percentage of intact acrosomes as the size of straw group increased. Our results indicate that, when using a nonthermostatically controlled thawing bath, semen packaged in .5-ml straws can be thawed in groups of 20 without an effect on post-thaw sperm viability.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>artificial insemination</subject><subject>bulls</subject><subject>Cattle - physiology</subject><subject>Cell Survival</subject><subject>congelacion</subject><subject>congelation</subject><subject>Cryopreservation - veterinary</subject><subject>decongelation</subject><subject>descongelacion</subject><subject>freezing</subject><subject>inseminacion artificial</subject><subject>insemination artificielle</subject><subject>Male</subject><subject>mouvement</subject><subject>movement</subject><subject>movimiento</subject><subject>semen</subject><subject>Semen Preservation - veterinary</subject><subject>Sperm Motility</subject><subject>Spermatozoa - physiology</subject><subject>sperme</subject><subject>taureau</subject><subject>Temperature</subject><subject>thawing</subject><subject>toro</subject><subject>viabilidad</subject><subject>viabilite</subject><subject>viability</subject><issn>0021-8812</issn><issn>1525-3163</issn><issn>0021-8812</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0E1v1DAQBmALgcpSOHIE-cQty4xdfx1R1QJSJQ7Qs-Vk7ayLEwc722359STaop48lh69M3oJeY-wZYKpz2gMbqWRjAN_eEE2KJhoOEr-kmwAGDZaI3tN3tR6B4BMGHFGzlBrwQVsSLoKwXczzYH2JR8mOu_dMY49zSOdcp2b9U_vo2tjivPj6tp8H0dP6-TL4Ob8Nzs6ue636_2OxpFuRTPElFbuC70ufuz2tM7FHetb8iq4VP27p_ec3F5f_br81tz8-Pr98stN0zGh5qZtcTlfGMXhYodSLrP3qFULjocLYEYBBFTSMx2CUVoYE1ir0UkQnXeBn5NPp9yp5D8HX2c7xNr5lNzo86FaxUAZQL7A5gS7kmstPtipxMGVR4tg13bt2q793-7iPzwFH9rB7571qc7nxfvY74-xeFsHl9Ki0d65Ko2Vdk1a4McTDC5b15dY7e3PdReAZpIz_g-0pYri</recordid><startdate>199106</startdate><enddate>199106</enddate><creator>Brown, D. W., Jr</creator><creator>Senger, P. L</creator><creator>Becker, W. C</creator><general>Am Soc Animal Sci</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199106</creationdate><title>Effect of group thawing on post-thaw viability of bovine spermatozoa packaged in .5-milliliter French straws</title><author>Brown, D. W., Jr ; Senger, P. L ; Becker, W. C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c257t-bb1163597304d166635ee187b0a3f4029700f176e28ff978599f2b81a605ceaf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>artificial insemination</topic><topic>bulls</topic><topic>Cattle - physiology</topic><topic>Cell Survival</topic><topic>congelacion</topic><topic>congelation</topic><topic>Cryopreservation - veterinary</topic><topic>decongelation</topic><topic>descongelacion</topic><topic>freezing</topic><topic>inseminacion artificial</topic><topic>insemination artificielle</topic><topic>Male</topic><topic>mouvement</topic><topic>movement</topic><topic>movimiento</topic><topic>semen</topic><topic>Semen Preservation - veterinary</topic><topic>Sperm Motility</topic><topic>Spermatozoa - physiology</topic><topic>sperme</topic><topic>taureau</topic><topic>Temperature</topic><topic>thawing</topic><topic>toro</topic><topic>viabilidad</topic><topic>viabilite</topic><topic>viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brown, D. W., Jr</creatorcontrib><creatorcontrib>Senger, P. L</creatorcontrib><creatorcontrib>Becker, W. C</creatorcontrib><creatorcontrib>Washington State University, Pullman</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of animal science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brown, D. W., Jr</au><au>Senger, P. L</au><au>Becker, W. C</au><aucorp>Washington State University, Pullman</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of group thawing on post-thaw viability of bovine spermatozoa packaged in .5-milliliter French straws</atitle><jtitle>Journal of animal science</jtitle><addtitle>J Anim Sci</addtitle><date>1991-06</date><risdate>1991</risdate><volume>69</volume><issue>6</issue><spage>2303</spage><epage>2309</epage><pages>2303-2309</pages><issn>0021-8812</issn><eissn>1525-3163</eissn><eissn>0021-8812</eissn><abstract>The objective of this study was to determine the effects of thawing groups of 2, 5, 10, 15, or 20 .5-ml French straws on post-thaw spermatozoal viability. Thermostatically controlled and nonthermostatically controlled thawing baths were compared. Using a split-plot design, semen from 10 bulls was extended in egg yolk citrate, frozen, and then thawed (in the respective groups) at 36 degrees C in two types of thawing baths. Motility and percentage of intact acrosomes were determined immediately after thawing (0 h) and again after 4 h of incubation at the respective temperature of each thawing bath. Neither percentage of intact acrosomes nor motility was influenced by the number of straws thawed at 0 h (P > .05). Thawing bath had no effect (P > .05) on motility or percentage of intact acrosomes at 0 h. Bull variation was significant in both the 0- and 4-h evaluations. After 4 h of incubation, there was a significant (P < .05) straw number X thawing bath interaction. When 15 or 20 straws were thawed in the thermostatically controlled bath there was a reduction (P < .05) in motility and percentage of intact acrosomes. However, in the nonthermostatically controlled bath there was no reduction in motility and percentage of intact acrosomes as the size of straw group increased. Our results indicate that, when using a nonthermostatically controlled thawing bath, semen packaged in .5-ml straws can be thawed in groups of 20 without an effect on post-thaw sperm viability.</abstract><cop>United States</cop><pub>Am Soc Animal Sci</pub><pmid>1885350</pmid><doi>10.2527/1991.6962303x</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0021-8812 |
| ispartof | Journal of animal science, 1991-06, Vol.69 (6), p.2303-2309 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Analysis of Variance Animals artificial insemination bulls Cattle - physiology Cell Survival congelacion congelation Cryopreservation - veterinary decongelation descongelacion freezing inseminacion artificial insemination artificielle Male mouvement movement movimiento semen Semen Preservation - veterinary Sperm Motility Spermatozoa - physiology sperme taureau Temperature thawing toro viabilidad viabilite viability |
| title | Effect of group thawing on post-thaw viability of bovine spermatozoa packaged in .5-milliliter French straws |
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