Detection of experimental autoimmune myocarditis in rats by 111In monoclonal antibody specific for tenascin-C
Although the identification of inflammatory infiltrates in endomyocardial biopsy specimens is necessary for the definite diagnosis of myocarditis, the biopsy test is invasive and is not sensitive. Therefore, a new diagnostic technique for the early and noninvasive evaluation of myocarditis has been...
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| Veröffentlicht in: | Circulation (New York, N.Y.) N.Y.), 2002-09, Vol.106 (11), p.1397-1402 |
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| creator | SATO, Mikio TOYOZAKI, Tetsuya TADOKORO, Hiroyuki IRIE, Toshiaki TANADA, Shuji KOMURO, Issei ODAKA, Kenichi UEHARA, Tomoya ARANO, Yasushi HASEGAWA, Hiroshi YOSHIDA, Katsuya IMANAKA-YOSHIDA, Kyoko YOSHIDA, Toshimichi HIROE, Michiaki |
| description | Although the identification of inflammatory infiltrates in endomyocardial biopsy specimens is necessary for the definite diagnosis of myocarditis, the biopsy test is invasive and is not sensitive. Therefore, a new diagnostic technique for the early and noninvasive evaluation of myocarditis has been awaited. Expression of tenascin-C (TNC), one of the oligometric extracellular glycoproteins, is induced in various pathological states, including inflammation, suggesting that TNC can be a molecular marker of myocarditis.
An 111In anti-TNC monoclonal antibody Fab' fragment was injected intravenously into rats with experimental autoimmune myocarditis (EAM), and the biodistribution of this radiotracer was measured. Rapid clearance of radioactivity from the blood was observed in both EAM and control rats ( |
| doi_str_mv | 10.1161/01.CIR.0000027823.07104.86 |
| format | Article |
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An 111In anti-TNC monoclonal antibody Fab' fragment was injected intravenously into rats with experimental autoimmune myocarditis (EAM), and the biodistribution of this radiotracer was measured. Rapid clearance of radioactivity from the blood was observed in both EAM and control rats (<1% at 6 hours after injection). Myocardial uptake of the tracer was much higher in EAM rats than in control rats (7.54-, 4.39-, and 3.51-fold at 6, 24, and 48 hours after injection, respectively). By autoradiography, high radioactivities were clearly observed in the regions indicative of inflammation in EAM rats. Single-photon emission CT imaging demonstrated the focal myocardial uptake of 111In anti-TNC Fab' in vivo.
Radiolabeled anti-TNC Fab' may be useful for the noninvasive diagnosis of myocarditis.</description><identifier>ISSN: 0009-7322</identifier><identifier>EISSN: 1524-4539</identifier><identifier>DOI: 10.1161/01.CIR.0000027823.07104.86</identifier><identifier>PMID: 12221059</identifier><identifier>CODEN: CIRCAZ</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Antibodies, Monoclonal - pharmacokinetics ; Autoimmune Diseases - diagnostic imaging ; Autoimmune Diseases - metabolism ; Autoimmune Diseases - pathology ; Autoradiography ; Biological and medical sciences ; Biomarkers - analysis ; Cardiovascular system ; Female ; Immunoglobulin Fab Fragments - metabolism ; Indium Radioisotopes ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Myocarditis - diagnostic imaging ; Myocarditis - immunology ; Myocarditis - metabolism ; Myocarditis - pathology ; Myocardium - metabolism ; Radionuclide investigations ; Rats ; Rats, Inbred Lew ; Tenascin - immunology ; Tenascin - metabolism ; Tomography, Emission-Computed, Single-Photon</subject><ispartof>Circulation (New York, N.Y.), 2002-09, Vol.106 (11), p.1397-1402</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2289-53abdb94828666753d40e5e275ccf0ddfdffd97d6ec532675244cd56f451153b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13901309$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12221059$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SATO, Mikio</creatorcontrib><creatorcontrib>TOYOZAKI, Tetsuya</creatorcontrib><creatorcontrib>TADOKORO, Hiroyuki</creatorcontrib><creatorcontrib>IRIE, Toshiaki</creatorcontrib><creatorcontrib>TANADA, Shuji</creatorcontrib><creatorcontrib>KOMURO, Issei</creatorcontrib><creatorcontrib>ODAKA, Kenichi</creatorcontrib><creatorcontrib>UEHARA, Tomoya</creatorcontrib><creatorcontrib>ARANO, Yasushi</creatorcontrib><creatorcontrib>HASEGAWA, Hiroshi</creatorcontrib><creatorcontrib>YOSHIDA, Katsuya</creatorcontrib><creatorcontrib>IMANAKA-YOSHIDA, Kyoko</creatorcontrib><creatorcontrib>YOSHIDA, Toshimichi</creatorcontrib><creatorcontrib>HIROE, Michiaki</creatorcontrib><title>Detection of experimental autoimmune myocarditis in rats by 111In monoclonal antibody specific for tenascin-C</title><title>Circulation (New York, N.Y.)</title><addtitle>Circulation</addtitle><description>Although the identification of inflammatory infiltrates in endomyocardial biopsy specimens is necessary for the definite diagnosis of myocarditis, the biopsy test is invasive and is not sensitive. Therefore, a new diagnostic technique for the early and noninvasive evaluation of myocarditis has been awaited. Expression of tenascin-C (TNC), one of the oligometric extracellular glycoproteins, is induced in various pathological states, including inflammation, suggesting that TNC can be a molecular marker of myocarditis.
An 111In anti-TNC monoclonal antibody Fab' fragment was injected intravenously into rats with experimental autoimmune myocarditis (EAM), and the biodistribution of this radiotracer was measured. Rapid clearance of radioactivity from the blood was observed in both EAM and control rats (<1% at 6 hours after injection). Myocardial uptake of the tracer was much higher in EAM rats than in control rats (7.54-, 4.39-, and 3.51-fold at 6, 24, and 48 hours after injection, respectively). By autoradiography, high radioactivities were clearly observed in the regions indicative of inflammation in EAM rats. Single-photon emission CT imaging demonstrated the focal myocardial uptake of 111In anti-TNC Fab' in vivo.
Radiolabeled anti-TNC Fab' may be useful for the noninvasive diagnosis of myocarditis.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Monoclonal - pharmacokinetics</subject><subject>Autoimmune Diseases - diagnostic imaging</subject><subject>Autoimmune Diseases - metabolism</subject><subject>Autoimmune Diseases - pathology</subject><subject>Autoradiography</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>Cardiovascular system</subject><subject>Female</subject><subject>Immunoglobulin Fab Fragments - metabolism</subject><subject>Indium Radioisotopes</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Myocarditis - diagnostic imaging</subject><subject>Myocarditis - immunology</subject><subject>Myocarditis - metabolism</subject><subject>Myocarditis - pathology</subject><subject>Myocardium - metabolism</subject><subject>Radionuclide investigations</subject><subject>Rats</subject><subject>Rats, Inbred Lew</subject><subject>Tenascin - immunology</subject><subject>Tenascin - metabolism</subject><subject>Tomography, Emission-Computed, Single-Photon</subject><issn>0009-7322</issn><issn>1524-4539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0EtLxDAQAOAgiruu_gUJgt5a82iS5ij1tbAgiJ5LmgdE2qQ2Kbj_3oorzmUY5puBGQCuMCox5vgW4bLZvpboJ4ioCS2RwKgqa34E1piRqqgYlcdgvfRlISghK3CW0sdScirYKVhhQghGTK7BcG-z1dnHAKOD9mu0kx9syKqHas7RD8McLBz2UavJ-OwT9AFOKifY7SHGeBvgEEPUfQw_IyH7Lpo9TKPV3nkNXZxgtkEl7UPRnIMTp_pkLw55A94fH96a52L38rRt7naFJqSWBaOqM52salJzzgWjpkKWWSKY1g4Z44xzRgrDrWaULIBUlTaMu4phzGhHN-Dmd-84xc_ZptwOPmnb9yrYOKdWECRqUfMFXh7g3A3WtONyvZr27d-DFnB9AMsJqneTCtqnf0clwhRJ-g3pvneN</recordid><startdate>20020910</startdate><enddate>20020910</enddate><creator>SATO, Mikio</creator><creator>TOYOZAKI, Tetsuya</creator><creator>TADOKORO, Hiroyuki</creator><creator>IRIE, Toshiaki</creator><creator>TANADA, Shuji</creator><creator>KOMURO, Issei</creator><creator>ODAKA, Kenichi</creator><creator>UEHARA, Tomoya</creator><creator>ARANO, Yasushi</creator><creator>HASEGAWA, Hiroshi</creator><creator>YOSHIDA, Katsuya</creator><creator>IMANAKA-YOSHIDA, Kyoko</creator><creator>YOSHIDA, Toshimichi</creator><creator>HIROE, Michiaki</creator><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20020910</creationdate><title>Detection of experimental autoimmune myocarditis in rats by 111In monoclonal antibody specific for tenascin-C</title><author>SATO, Mikio ; TOYOZAKI, Tetsuya ; TADOKORO, Hiroyuki ; IRIE, Toshiaki ; TANADA, Shuji ; KOMURO, Issei ; ODAKA, Kenichi ; UEHARA, Tomoya ; ARANO, Yasushi ; HASEGAWA, Hiroshi ; YOSHIDA, Katsuya ; IMANAKA-YOSHIDA, Kyoko ; YOSHIDA, Toshimichi ; HIROE, Michiaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2289-53abdb94828666753d40e5e275ccf0ddfdffd97d6ec532675244cd56f451153b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Monoclonal - pharmacokinetics</topic><topic>Autoimmune Diseases - diagnostic imaging</topic><topic>Autoimmune Diseases - metabolism</topic><topic>Autoimmune Diseases - pathology</topic><topic>Autoradiography</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - analysis</topic><topic>Cardiovascular system</topic><topic>Female</topic><topic>Immunoglobulin Fab Fragments - metabolism</topic><topic>Indium Radioisotopes</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Myocarditis - diagnostic imaging</topic><topic>Myocarditis - immunology</topic><topic>Myocarditis - metabolism</topic><topic>Myocarditis - pathology</topic><topic>Myocardium - metabolism</topic><topic>Radionuclide investigations</topic><topic>Rats</topic><topic>Rats, Inbred Lew</topic><topic>Tenascin - immunology</topic><topic>Tenascin - metabolism</topic><topic>Tomography, Emission-Computed, Single-Photon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SATO, Mikio</creatorcontrib><creatorcontrib>TOYOZAKI, Tetsuya</creatorcontrib><creatorcontrib>TADOKORO, Hiroyuki</creatorcontrib><creatorcontrib>IRIE, Toshiaki</creatorcontrib><creatorcontrib>TANADA, Shuji</creatorcontrib><creatorcontrib>KOMURO, Issei</creatorcontrib><creatorcontrib>ODAKA, Kenichi</creatorcontrib><creatorcontrib>UEHARA, Tomoya</creatorcontrib><creatorcontrib>ARANO, Yasushi</creatorcontrib><creatorcontrib>HASEGAWA, Hiroshi</creatorcontrib><creatorcontrib>YOSHIDA, Katsuya</creatorcontrib><creatorcontrib>IMANAKA-YOSHIDA, Kyoko</creatorcontrib><creatorcontrib>YOSHIDA, Toshimichi</creatorcontrib><creatorcontrib>HIROE, Michiaki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Circulation (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SATO, Mikio</au><au>TOYOZAKI, Tetsuya</au><au>TADOKORO, Hiroyuki</au><au>IRIE, Toshiaki</au><au>TANADA, Shuji</au><au>KOMURO, Issei</au><au>ODAKA, Kenichi</au><au>UEHARA, Tomoya</au><au>ARANO, Yasushi</au><au>HASEGAWA, Hiroshi</au><au>YOSHIDA, Katsuya</au><au>IMANAKA-YOSHIDA, Kyoko</au><au>YOSHIDA, Toshimichi</au><au>HIROE, Michiaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of experimental autoimmune myocarditis in rats by 111In monoclonal antibody specific for tenascin-C</atitle><jtitle>Circulation (New York, N.Y.)</jtitle><addtitle>Circulation</addtitle><date>2002-09-10</date><risdate>2002</risdate><volume>106</volume><issue>11</issue><spage>1397</spage><epage>1402</epage><pages>1397-1402</pages><issn>0009-7322</issn><eissn>1524-4539</eissn><coden>CIRCAZ</coden><abstract>Although the identification of inflammatory infiltrates in endomyocardial biopsy specimens is necessary for the definite diagnosis of myocarditis, the biopsy test is invasive and is not sensitive. Therefore, a new diagnostic technique for the early and noninvasive evaluation of myocarditis has been awaited. Expression of tenascin-C (TNC), one of the oligometric extracellular glycoproteins, is induced in various pathological states, including inflammation, suggesting that TNC can be a molecular marker of myocarditis.
An 111In anti-TNC monoclonal antibody Fab' fragment was injected intravenously into rats with experimental autoimmune myocarditis (EAM), and the biodistribution of this radiotracer was measured. Rapid clearance of radioactivity from the blood was observed in both EAM and control rats (<1% at 6 hours after injection). Myocardial uptake of the tracer was much higher in EAM rats than in control rats (7.54-, 4.39-, and 3.51-fold at 6, 24, and 48 hours after injection, respectively). By autoradiography, high radioactivities were clearly observed in the regions indicative of inflammation in EAM rats. Single-photon emission CT imaging demonstrated the focal myocardial uptake of 111In anti-TNC Fab' in vivo.
Radiolabeled anti-TNC Fab' may be useful for the noninvasive diagnosis of myocarditis.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>12221059</pmid><doi>10.1161/01.CIR.0000027823.07104.86</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; American Heart Association Journals; Journals@Ovid Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Antibodies, Monoclonal - immunology Antibodies, Monoclonal - pharmacokinetics Autoimmune Diseases - diagnostic imaging Autoimmune Diseases - metabolism Autoimmune Diseases - pathology Autoradiography Biological and medical sciences Biomarkers - analysis Cardiovascular system Female Immunoglobulin Fab Fragments - metabolism Indium Radioisotopes Investigative techniques, diagnostic techniques (general aspects) Medical sciences Myocarditis - diagnostic imaging Myocarditis - immunology Myocarditis - metabolism Myocarditis - pathology Myocardium - metabolism Radionuclide investigations Rats Rats, Inbred Lew Tenascin - immunology Tenascin - metabolism Tomography, Emission-Computed, Single-Photon |
| title | Detection of experimental autoimmune myocarditis in rats by 111In monoclonal antibody specific for tenascin-C |
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