Gram-negative bacteria killed by complement are associated with more severe biliary infections and produce more tumor necrosis factor-α in sera
Background. We previously showed that gallstones contain bacteria and that illness severity correlates with bacterial presence. This study examined virulence differences of gram-negative biliary bacteria. Methods. Gallstones and bile were cultured, and sera obtained, from 210 patients. Infection sev...
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| Veröffentlicht in: | Surgery 2002-08, Vol.132 (2), p.408-414 |
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| creator | Stewart, Lygia Oesterle, Adair L. Griffiss, J.McLeod Jarvis, Gary A. Aagaard, Beverly Way, Lawrence W. |
| description | Background. We previously showed that gallstones contain bacteria and that illness severity correlates with bacterial presence. This study examined virulence differences of gram-negative biliary bacteria. Methods. Gallstones and bile were cultured, and sera obtained, from 210 patients. Infection severity was staged as: none—no clinical infection; moderate—fever, leukocytosis; or severe—bacteremia, cholangitis, hypotension, abscess, or organ failure. Gram-negative biliary bacteria were tested against patient (and control) serum for complement-mediated bacterial killing and induction of tumor necrosis factor-α (TNFα) production (using cultured monocytes) with and without sera. These results were correlated with infection severity. Results. A total of 98 (47%) patients had biliary bacteria. Infection severity distribution was none, 29%; moderate, 35%; and severe, 36%. Gram-negative organisms killed by complement were associated with more severe infections as follows: 13%, none; 60%, moderate; and 88%, severe infections (P =.024 and P |
| doi_str_mv | 10.1067/msy.2002.127423 |
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| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72077547</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0039606002001034</els_id><sourcerecordid>72077547</sourcerecordid><originalsourceid>FETCH-LOGICAL-c373t-1dd22f39cda14dd48731ce3746d5013c25e9544011c7c963340262585268a40b3</originalsourceid><addsrcrecordid>eNp1kc9u1DAQxi1ERZfCmRvyBW7Zjv8k3hxRBQWpEpdythx7AobEXmxn0b5FX6UvwjPhVVbqidNI9m---WY-Qt4w2DLo1PWcj1sOwLeMK8nFM7JhreCNEh17TjYAom866OCSvMz5JwD0ku1ekEvGOetB8g15uE1mbgJ-N8UfkA7GFkze0F9-mtDR4UhtnPcTzhgKNQmpyTlab0r9_OPLDzrH-pjxgLUMfvImHakPI9riY8jUBEf3KbrF4oqWpRYa0KaYfaZjHRhT8_exNlWZZF6Ri9FMGV-f6xX59unj_c3n5u7r7ZebD3eNFUqUhjnH-Sh66wyTzsmdEsyiULJzLTBheYt9KyUwZpXtOyEk8I63u5Z3OyNhEFfk_apb3f1eMBc9-2xxmkzAuGStOCjVSlXB6xU8Oc4JR71Pfq5ragb6FIKuIehTCHoNoXa8PUsvw4zuiT9fvQLvzoDJ1kxjMsH6_MSJngvWnkb3K4f1EAePSWfrMVh0PtUDaxf9f038A8QZpRM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72077547</pqid></control><display><type>article</type><title>Gram-negative bacteria killed by complement are associated with more severe biliary infections and produce more tumor necrosis factor-α in sera</title><source>ScienceDirect</source><source>MEDLINE</source><creator>Stewart, Lygia ; Oesterle, Adair L. ; Griffiss, J.McLeod ; Jarvis, Gary A. ; Aagaard, Beverly ; Way, Lawrence W.</creator><creatorcontrib>Stewart, Lygia ; Oesterle, Adair L. ; Griffiss, J.McLeod ; Jarvis, Gary A. ; Aagaard, Beverly ; Way, Lawrence W.</creatorcontrib><description>Background. We previously showed that gallstones contain bacteria and that illness severity correlates with bacterial presence. This study examined virulence differences of gram-negative biliary bacteria. Methods. Gallstones and bile were cultured, and sera obtained, from 210 patients. Infection severity was staged as: none—no clinical infection; moderate—fever, leukocytosis; or severe—bacteremia, cholangitis, hypotension, abscess, or organ failure. Gram-negative biliary bacteria were tested against patient (and control) serum for complement-mediated bacterial killing and induction of tumor necrosis factor-α (TNFα) production (using cultured monocytes) with and without sera. These results were correlated with infection severity. Results. A total of 98 (47%) patients had biliary bacteria. Infection severity distribution was none, 29%; moderate, 35%; and severe, 36%. Gram-negative organisms killed by complement were associated with more severe infections as follows: 13%, none; 60%, moderate; and 88%, severe infections (P =.024 and P <.0001, respectively vs none, chi-square test). TNFα production in sera increased 182 pg/mL with complement resistant bacteria, but increased 546 pg/mL with bacteria killed by complement (P <.0001, killed vs not killed, Student's t test). E coli and Klebsiella were the most virulent bacterial species. They were cultured from blood, usually killed by complement, and had the largest increase in TNFα production in sera. Conclusions. Gram-negative biliary bacteria killed by complement (as opposed to complement-resistant) were associated with more serious biliary infections including bacteremia and induced more TNFα production in sera. This suggests a potential role for complement activation and cytokine production in biliary sepsis. (Surgery 2002;132:408-14.)</description><identifier>ISSN: 0039-6060</identifier><identifier>EISSN: 1532-7361</identifier><identifier>DOI: 10.1067/msy.2002.127423</identifier><identifier>PMID: 12219042</identifier><identifier>CODEN: SURGAZ</identifier><language>eng</language><publisher>New York, NY: Mosby, Inc</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Biliary Tract - immunology ; Biliary Tract - microbiology ; Biological and medical sciences ; Cholelithiasis - immunology ; Cholelithiasis - microbiology ; Complement Activation - immunology ; Complement System Proteins - immunology ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; Gram-Negative Bacteria - immunology ; Gram-Negative Bacterial Infections - immunology ; Humans ; In Vitro Techniques ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Male ; Medical sciences ; Middle Aged ; Other diseases. Semiology ; Tumor Necrosis Factor-alpha - biosynthesis ; Tumor Necrosis Factor-alpha - metabolism</subject><ispartof>Surgery, 2002-08, Vol.132 (2), p.408-414</ispartof><rights>2002</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-1dd22f39cda14dd48731ce3746d5013c25e9544011c7c963340262585268a40b3</citedby><cites>FETCH-LOGICAL-c373t-1dd22f39cda14dd48731ce3746d5013c25e9544011c7c963340262585268a40b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0039606002001034$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3537,23909,23910,25118,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13923157$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12219042$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stewart, Lygia</creatorcontrib><creatorcontrib>Oesterle, Adair L.</creatorcontrib><creatorcontrib>Griffiss, J.McLeod</creatorcontrib><creatorcontrib>Jarvis, Gary A.</creatorcontrib><creatorcontrib>Aagaard, Beverly</creatorcontrib><creatorcontrib>Way, Lawrence W.</creatorcontrib><title>Gram-negative bacteria killed by complement are associated with more severe biliary infections and produce more tumor necrosis factor-α in sera</title><title>Surgery</title><addtitle>Surgery</addtitle><description>Background. We previously showed that gallstones contain bacteria and that illness severity correlates with bacterial presence. This study examined virulence differences of gram-negative biliary bacteria. Methods. Gallstones and bile were cultured, and sera obtained, from 210 patients. Infection severity was staged as: none—no clinical infection; moderate—fever, leukocytosis; or severe—bacteremia, cholangitis, hypotension, abscess, or organ failure. Gram-negative biliary bacteria were tested against patient (and control) serum for complement-mediated bacterial killing and induction of tumor necrosis factor-α (TNFα) production (using cultured monocytes) with and without sera. These results were correlated with infection severity. Results. A total of 98 (47%) patients had biliary bacteria. Infection severity distribution was none, 29%; moderate, 35%; and severe, 36%. Gram-negative organisms killed by complement were associated with more severe infections as follows: 13%, none; 60%, moderate; and 88%, severe infections (P =.024 and P <.0001, respectively vs none, chi-square test). TNFα production in sera increased 182 pg/mL with complement resistant bacteria, but increased 546 pg/mL with bacteria killed by complement (P <.0001, killed vs not killed, Student's t test). E coli and Klebsiella were the most virulent bacterial species. They were cultured from blood, usually killed by complement, and had the largest increase in TNFα production in sera. Conclusions. Gram-negative biliary bacteria killed by complement (as opposed to complement-resistant) were associated with more serious biliary infections including bacteremia and induced more TNFα production in sera. This suggests a potential role for complement activation and cytokine production in biliary sepsis. (Surgery 2002;132:408-14.)</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biliary Tract - immunology</subject><subject>Biliary Tract - microbiology</subject><subject>Biological and medical sciences</subject><subject>Cholelithiasis - immunology</subject><subject>Cholelithiasis - microbiology</subject><subject>Complement Activation - immunology</subject><subject>Complement System Proteins - immunology</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Gram-Negative Bacteria - immunology</subject><subject>Gram-Negative Bacterial Infections - immunology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Other diseases. Semiology</subject><subject>Tumor Necrosis Factor-alpha - biosynthesis</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><issn>0039-6060</issn><issn>1532-7361</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc9u1DAQxi1ERZfCmRvyBW7Zjv8k3hxRBQWpEpdythx7AobEXmxn0b5FX6UvwjPhVVbqidNI9m---WY-Qt4w2DLo1PWcj1sOwLeMK8nFM7JhreCNEh17TjYAom866OCSvMz5JwD0ku1ekEvGOetB8g15uE1mbgJ-N8UfkA7GFkze0F9-mtDR4UhtnPcTzhgKNQmpyTlab0r9_OPLDzrH-pjxgLUMfvImHakPI9riY8jUBEf3KbrF4oqWpRYa0KaYfaZjHRhT8_exNlWZZF6Ri9FMGV-f6xX59unj_c3n5u7r7ZebD3eNFUqUhjnH-Sh66wyTzsmdEsyiULJzLTBheYt9KyUwZpXtOyEk8I63u5Z3OyNhEFfk_apb3f1eMBc9-2xxmkzAuGStOCjVSlXB6xU8Oc4JR71Pfq5ragb6FIKuIehTCHoNoXa8PUsvw4zuiT9fvQLvzoDJ1kxjMsH6_MSJngvWnkb3K4f1EAePSWfrMVh0PtUDaxf9f038A8QZpRM</recordid><startdate>20020801</startdate><enddate>20020801</enddate><creator>Stewart, Lygia</creator><creator>Oesterle, Adair L.</creator><creator>Griffiss, J.McLeod</creator><creator>Jarvis, Gary A.</creator><creator>Aagaard, Beverly</creator><creator>Way, Lawrence W.</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020801</creationdate><title>Gram-negative bacteria killed by complement are associated with more severe biliary infections and produce more tumor necrosis factor-α in sera</title><author>Stewart, Lygia ; Oesterle, Adair L. ; Griffiss, J.McLeod ; Jarvis, Gary A. ; Aagaard, Beverly ; Way, Lawrence W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-1dd22f39cda14dd48731ce3746d5013c25e9544011c7c963340262585268a40b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biliary Tract - immunology</topic><topic>Biliary Tract - microbiology</topic><topic>Biological and medical sciences</topic><topic>Cholelithiasis - immunology</topic><topic>Cholelithiasis - microbiology</topic><topic>Complement Activation - immunology</topic><topic>Complement System Proteins - immunology</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Gram-Negative Bacteria - immunology</topic><topic>Gram-Negative Bacterial Infections - immunology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Other diseases. Semiology</topic><topic>Tumor Necrosis Factor-alpha - biosynthesis</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stewart, Lygia</creatorcontrib><creatorcontrib>Oesterle, Adair L.</creatorcontrib><creatorcontrib>Griffiss, J.McLeod</creatorcontrib><creatorcontrib>Jarvis, Gary A.</creatorcontrib><creatorcontrib>Aagaard, Beverly</creatorcontrib><creatorcontrib>Way, Lawrence W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stewart, Lygia</au><au>Oesterle, Adair L.</au><au>Griffiss, J.McLeod</au><au>Jarvis, Gary A.</au><au>Aagaard, Beverly</au><au>Way, Lawrence W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gram-negative bacteria killed by complement are associated with more severe biliary infections and produce more tumor necrosis factor-α in sera</atitle><jtitle>Surgery</jtitle><addtitle>Surgery</addtitle><date>2002-08-01</date><risdate>2002</risdate><volume>132</volume><issue>2</issue><spage>408</spage><epage>414</epage><pages>408-414</pages><issn>0039-6060</issn><eissn>1532-7361</eissn><coden>SURGAZ</coden><abstract>Background. We previously showed that gallstones contain bacteria and that illness severity correlates with bacterial presence. This study examined virulence differences of gram-negative biliary bacteria. Methods. Gallstones and bile were cultured, and sera obtained, from 210 patients. Infection severity was staged as: none—no clinical infection; moderate—fever, leukocytosis; or severe—bacteremia, cholangitis, hypotension, abscess, or organ failure. Gram-negative biliary bacteria were tested against patient (and control) serum for complement-mediated bacterial killing and induction of tumor necrosis factor-α (TNFα) production (using cultured monocytes) with and without sera. These results were correlated with infection severity. Results. A total of 98 (47%) patients had biliary bacteria. Infection severity distribution was none, 29%; moderate, 35%; and severe, 36%. Gram-negative organisms killed by complement were associated with more severe infections as follows: 13%, none; 60%, moderate; and 88%, severe infections (P =.024 and P <.0001, respectively vs none, chi-square test). TNFα production in sera increased 182 pg/mL with complement resistant bacteria, but increased 546 pg/mL with bacteria killed by complement (P <.0001, killed vs not killed, Student's t test). E coli and Klebsiella were the most virulent bacterial species. They were cultured from blood, usually killed by complement, and had the largest increase in TNFα production in sera. Conclusions. Gram-negative biliary bacteria killed by complement (as opposed to complement-resistant) were associated with more serious biliary infections including bacteremia and induced more TNFα production in sera. This suggests a potential role for complement activation and cytokine production in biliary sepsis. (Surgery 2002;132:408-14.)</abstract><cop>New York, NY</cop><pub>Mosby, Inc</pub><pmid>12219042</pmid><doi>10.1067/msy.2002.127423</doi><tpages>7</tpages></addata></record> |
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| subjects | Adolescent Adult Aged Aged, 80 and over Biliary Tract - immunology Biliary Tract - microbiology Biological and medical sciences Cholelithiasis - immunology Cholelithiasis - microbiology Complement Activation - immunology Complement System Proteins - immunology Female Gastroenterology. Liver. Pancreas. Abdomen Gram-Negative Bacteria - immunology Gram-Negative Bacterial Infections - immunology Humans In Vitro Techniques Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Medical sciences Middle Aged Other diseases. Semiology Tumor Necrosis Factor-alpha - biosynthesis Tumor Necrosis Factor-alpha - metabolism |
| title | Gram-negative bacteria killed by complement are associated with more severe biliary infections and produce more tumor necrosis factor-α in sera |
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