Virus‐induced gene silencing in tomato
We have previously demonstrated that a tobacco rattle virus (TRV)‐based vector can be used in virus‐induced gene silencing (VIGS) to study gene function in Nicotiana benthamiana. Here we show that recombinant TRV infects tomato plants and induces efficient gene silencing. Using this system, we suppr...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2002-09, Vol.31 (6), p.777-786 |
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| container_title | The Plant journal : for cell and molecular biology |
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| creator | Liu, Yule Schiff, Michael Dinesh‐Kumar, S. P. |
| description | We have previously demonstrated that a tobacco rattle virus (TRV)‐based vector can be used in virus‐induced gene silencing (VIGS) to study gene function in Nicotiana benthamiana. Here we show that recombinant TRV infects tomato plants and induces efficient gene silencing. Using this system, we suppressed the PDS, CTR1 and CTR2 genes in tomato. Suppression of CTR1 led to a constitutive ethylene response phenotype and up‐regulation of an ethylene response gene, CHITINASE B. This phenotype is similar to Arabidopsis ctr1 mutant plants. We have constructed a modified TRV vector based on the GATEWAY recombination system, allowing restriction‐ and ligation‐free cloning. Our results show that tomato expressed sequence tags (ESTs) can easily be cloned into this modified vector using a single set of primers. Using this vector, we have silenced RbcS and an endogenous gene homologous to the tomato EST cLED3L14. In the future, this modified vector system will facilitate large‐scale functional analysis of tomato ESTs. |
| doi_str_mv | 10.1046/j.1365-313X.2002.01394.x |
| format | Article |
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P.</creator><creatorcontrib>Liu, Yule ; Schiff, Michael ; Dinesh‐Kumar, S. P.</creatorcontrib><description>We have previously demonstrated that a tobacco rattle virus (TRV)‐based vector can be used in virus‐induced gene silencing (VIGS) to study gene function in Nicotiana benthamiana. Here we show that recombinant TRV infects tomato plants and induces efficient gene silencing. Using this system, we suppressed the PDS, CTR1 and CTR2 genes in tomato. Suppression of CTR1 led to a constitutive ethylene response phenotype and up‐regulation of an ethylene response gene, CHITINASE B. This phenotype is similar to Arabidopsis ctr1 mutant plants. We have constructed a modified TRV vector based on the GATEWAY recombination system, allowing restriction‐ and ligation‐free cloning. Our results show that tomato expressed sequence tags (ESTs) can easily be cloned into this modified vector using a single set of primers. Using this vector, we have silenced RbcS and an endogenous gene homologous to the tomato EST cLED3L14. In the future, this modified vector system will facilitate large‐scale functional analysis of tomato ESTs.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1046/j.1365-313X.2002.01394.x</identifier><identifier>PMID: 12220268</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Biological and medical sciences ; Carrier Proteins - genetics ; Cation Transport Proteins ; Chitinases - genetics ; Cloning, Molecular ; Copper Transporter 1 ; Ethylenes - pharmacology ; functional genomics ; Fundamental and applied biological sciences. Psychology ; GATEWAY cloning ; Gene expression ; Gene Expression Regulation, Plant ; Gene Silencing ; Genetic Vectors - genetics ; Membrane Proteins - genetics ; Membrane Transport Proteins ; Molecular and cellular biology ; Molecular genetics ; Mutation ; Nicotiana - virology ; Phenotype ; Plant Viruses - genetics ; Plant Viruses - growth & development ; Plasmids - genetics ; Protein Kinases ; Rhizobium - genetics ; Solanum lycopersicum - genetics ; Solanum lycopersicum - virology ; Sulfate Transporters ; tCTR1 ; tobacco rattle virus vector ; tomato</subject><ispartof>The Plant journal : for cell and molecular biology, 2002-09, Vol.31 (6), p.777-786</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5474-26557101190416c6af41b225e05b71481fd9d7f7d56932d2108999264fea04263</citedby><cites>FETCH-LOGICAL-c5474-26557101190416c6af41b225e05b71481fd9d7f7d56932d2108999264fea04263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-313X.2002.01394.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-313X.2002.01394.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13903439$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12220268$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Yule</creatorcontrib><creatorcontrib>Schiff, Michael</creatorcontrib><creatorcontrib>Dinesh‐Kumar, S. P.</creatorcontrib><title>Virus‐induced gene silencing in tomato</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>We have previously demonstrated that a tobacco rattle virus (TRV)‐based vector can be used in virus‐induced gene silencing (VIGS) to study gene function in Nicotiana benthamiana. Here we show that recombinant TRV infects tomato plants and induces efficient gene silencing. Using this system, we suppressed the PDS, CTR1 and CTR2 genes in tomato. Suppression of CTR1 led to a constitutive ethylene response phenotype and up‐regulation of an ethylene response gene, CHITINASE B. This phenotype is similar to Arabidopsis ctr1 mutant plants. We have constructed a modified TRV vector based on the GATEWAY recombination system, allowing restriction‐ and ligation‐free cloning. Our results show that tomato expressed sequence tags (ESTs) can easily be cloned into this modified vector using a single set of primers. Using this vector, we have silenced RbcS and an endogenous gene homologous to the tomato EST cLED3L14. In the future, this modified vector system will facilitate large‐scale functional analysis of tomato ESTs.</description><subject>Biological and medical sciences</subject><subject>Carrier Proteins - genetics</subject><subject>Cation Transport Proteins</subject><subject>Chitinases - genetics</subject><subject>Cloning, Molecular</subject><subject>Copper Transporter 1</subject><subject>Ethylenes - pharmacology</subject><subject>functional genomics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GATEWAY cloning</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Silencing</subject><subject>Genetic Vectors - genetics</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Transport Proteins</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Mutation</subject><subject>Nicotiana - virology</subject><subject>Phenotype</subject><subject>Plant Viruses - genetics</subject><subject>Plant Viruses - growth & development</subject><subject>Plasmids - genetics</subject><subject>Protein Kinases</subject><subject>Rhizobium - genetics</subject><subject>Solanum lycopersicum - genetics</subject><subject>Solanum lycopersicum - virology</subject><subject>Sulfate Transporters</subject><subject>tCTR1</subject><subject>tobacco rattle virus vector</subject><subject>tomato</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtOwzAQhi0EoqVwBZQNiE3CjO3Y8YIFqniqEiwKYme5iVOlygPiRrQ7jsAZOQkJjegSVjPSfP_M6CPEQwgQuDhfBMhE6DNkLwEFoAEgUzxY7ZDh72CXDEEJ8CVHOiAHzi0AUDLB98kAKaVARTQkZ89Z3bivj8-sTJrYJt7cltZzWW7LOCvnXlZ6y6owy-qQ7KUmd_aoryPydH01Hd_6k4ebu_HlxI9DLrlPRRhKBEQFHEUsTMpxRmloIZxJ5BGmiUpkKpNQKEYTihAppajgqTXAqWAjcrrZ-1pXb411S11kLrZ5bkpbNU5LClIyzv8EMeJKCug2Rhswrivnapvq1zorTL3WCLrTqRe6s6Y7a7rTqX906lUbPe5vNLPCJttg768FTnrAuNjkaW1abW7LMQWMM9VyFxvuvTW7_vcDevp433XsGx2jjiI</recordid><startdate>200209</startdate><enddate>200209</enddate><creator>Liu, Yule</creator><creator>Schiff, Michael</creator><creator>Dinesh‐Kumar, S. P.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>200209</creationdate><title>Virus‐induced gene silencing in tomato</title><author>Liu, Yule ; Schiff, Michael ; Dinesh‐Kumar, S. P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5474-26557101190416c6af41b225e05b71481fd9d7f7d56932d2108999264fea04263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Biological and medical sciences</topic><topic>Carrier Proteins - genetics</topic><topic>Cation Transport Proteins</topic><topic>Chitinases - genetics</topic><topic>Cloning, Molecular</topic><topic>Copper Transporter 1</topic><topic>Ethylenes - pharmacology</topic><topic>functional genomics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GATEWAY cloning</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Silencing</topic><topic>Genetic Vectors - genetics</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Transport Proteins</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Mutation</topic><topic>Nicotiana - virology</topic><topic>Phenotype</topic><topic>Plant Viruses - genetics</topic><topic>Plant Viruses - growth & development</topic><topic>Plasmids - genetics</topic><topic>Protein Kinases</topic><topic>Rhizobium - genetics</topic><topic>Solanum lycopersicum - genetics</topic><topic>Solanum lycopersicum - virology</topic><topic>Sulfate Transporters</topic><topic>tCTR1</topic><topic>tobacco rattle virus vector</topic><topic>tomato</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Yule</creatorcontrib><creatorcontrib>Schiff, Michael</creatorcontrib><creatorcontrib>Dinesh‐Kumar, S. P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Yule</au><au>Schiff, Michael</au><au>Dinesh‐Kumar, S. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Virus‐induced gene silencing in tomato</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2002-09</date><risdate>2002</risdate><volume>31</volume><issue>6</issue><spage>777</spage><epage>786</epage><pages>777-786</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>We have previously demonstrated that a tobacco rattle virus (TRV)‐based vector can be used in virus‐induced gene silencing (VIGS) to study gene function in Nicotiana benthamiana. Here we show that recombinant TRV infects tomato plants and induces efficient gene silencing. Using this system, we suppressed the PDS, CTR1 and CTR2 genes in tomato. Suppression of CTR1 led to a constitutive ethylene response phenotype and up‐regulation of an ethylene response gene, CHITINASE B. This phenotype is similar to Arabidopsis ctr1 mutant plants. We have constructed a modified TRV vector based on the GATEWAY recombination system, allowing restriction‐ and ligation‐free cloning. Our results show that tomato expressed sequence tags (ESTs) can easily be cloned into this modified vector using a single set of primers. Using this vector, we have silenced RbcS and an endogenous gene homologous to the tomato EST cLED3L14. In the future, this modified vector system will facilitate large‐scale functional analysis of tomato ESTs.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12220268</pmid><doi>10.1046/j.1365-313X.2002.01394.x</doi><tpages>10</tpages></addata></record> |
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| source | Wiley Free Content; MEDLINE; IngentaConnect Free/Open Access Journals; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Biological and medical sciences Carrier Proteins - genetics Cation Transport Proteins Chitinases - genetics Cloning, Molecular Copper Transporter 1 Ethylenes - pharmacology functional genomics Fundamental and applied biological sciences. Psychology GATEWAY cloning Gene expression Gene Expression Regulation, Plant Gene Silencing Genetic Vectors - genetics Membrane Proteins - genetics Membrane Transport Proteins Molecular and cellular biology Molecular genetics Mutation Nicotiana - virology Phenotype Plant Viruses - genetics Plant Viruses - growth & development Plasmids - genetics Protein Kinases Rhizobium - genetics Solanum lycopersicum - genetics Solanum lycopersicum - virology Sulfate Transporters tCTR1 tobacco rattle virus vector tomato |
| title | Virus‐induced gene silencing in tomato |
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