Functional and molecular evidence for Na(+)-HCO cotransporter in porcine vas deferens epithelia

This study focused on the role of sodium-bicarbonate cotransporter (NBC1) in cAMP-stimulated ion transport in porcine vas deferens epithelium. Ion substitution experiments in modified Ussing chambers revealed that cAMP-mediated stimulation was dependent on the presence of Na(+), HCO, and Cl(-) for a...

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Veröffentlicht in:	American Journal of Physiology: Cell Physiology 2002-10, Vol.283 (4), p.C1033-C1044
Hauptverfasser:	Carlin, Ryan W, Quesnell, Rebecca R, Zheng, Ling, Mitchell, Kathy E, Schultz, Bruce D
Format:	Artikel
Sprache:	eng
Schlagworte:	4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid - pharmacology Animals Base Sequence Bicarbonates - metabolism Carbonic Anhydrase Inhibitors - pharmacology Cells, Cultured Chlorides - metabolism Colforsin - pharmacology Cyclic AMP - metabolism Diffusion Chambers, Culture Epithelial Cells - cytology Epithelial Cells - drug effects Epithelial Cells - metabolism Epitopes - immunology Immunoblotting Ion Transport - drug effects Ion Transport - physiology Male Molecular Sequence Data Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis RNA, Messenger - biosynthesis Sequence Homology, Amino Acid Sodium - metabolism Sodium-Bicarbonate Symporters - drug effects Sodium-Bicarbonate Symporters - genetics Sodium-Bicarbonate Symporters - metabolism Stilbenes - pharmacology Swine Vas Deferens - cytology Vas Deferens - metabolism
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