Effects of Citrus aurantifolia concentrated extract on the spontaneous proliferation of MDA-MB-453 and RPMI-8866 tumor cell lines
The in vitro effects of concentrated lime juice (CLJ) extract on the spontaneous proliferation of a human breast carcinoma cell line (MDA-MB-453) and a human lymphoblastoid B cell line (RPMI-8866) were investigated. CLJ extract was prepared by freeze-drying fresh fruit juice and dialyzing the concen...
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| Veröffentlicht in: | Phytomedicine (Stuttgart) 2002-07, Vol.9 (5), p.475-477 |
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| creator | Gharagozloo, M. Doroudchi, M. Ghaderi, A. |
| description | The in vitro effects of concentrated lime juice (CLJ) extract on the spontaneous proliferation of a human breast carcinoma cell line (MDA-MB-453) and a human lymphoblastoid B cell line (RPMI-8866) were investigated. CLJ extract was prepared by freeze-drying fresh fruit juice and dialyzing the concentrated extract against phosphate buffered saline in order to deplete low molecular weight micronutrients such as flavonoids as well as adjusting the pH of the extract to the physiological range. The effects of different concentrations of the CLJ extract on the spontaneous proliferative responses of the cell lines were determined by
3H-thymidine incorporation after 24 hrs of incubation. CLJ extract had no significant effect on MDA-MB-453 cell line, however, using the concentrations of 125, 250, and 500 μg/ml of CLJ extract a significant inhibition of the spontaneous proliferation of RPMI-8866 cell line was detected (
P < 0.05). Due to the protein nature of the biologically active macromolecules of the CLJ extract (Gharagozloo and Ghaderi, 2001), it is reasonable to assume that the protein components of the CLJ extract may have anti-proliferative effects on tumor cell lines. |
| doi_str_mv | 10.1078/09447110260571751 |
| format | Article |
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3H-thymidine incorporation after 24 hrs of incubation. CLJ extract had no significant effect on MDA-MB-453 cell line, however, using the concentrations of 125, 250, and 500 μg/ml of CLJ extract a significant inhibition of the spontaneous proliferation of RPMI-8866 cell line was detected (
P < 0.05). Due to the protein nature of the biologically active macromolecules of the CLJ extract (Gharagozloo and Ghaderi, 2001), it is reasonable to assume that the protein components of the CLJ extract may have anti-proliferative effects on tumor cell lines.</description><identifier>ISSN: 0944-7113</identifier><identifier>EISSN: 1618-095X</identifier><identifier>DOI: 10.1078/09447110260571751</identifier><identifier>PMID: 12222671</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Alternative medicine ; antitumoraleffect ; Breast cancer ; Care and treatment ; Cell Division - drug effects ; Chromatography, High Pressure Liquid ; Citrus aurantifolia ; Citrus aurantiifolia - chemistry ; Evaluation ; Health aspects ; Humans ; In Vitro Techniques ; lime ; Lime oil ; MDA-MB-453 ; Medicinal plants ; Plant Extracts - pharmacology ; protein ; RPMI-8866 ; Tumor Cells, Cultured</subject><ispartof>Phytomedicine (Stuttgart), 2002-07, Vol.9 (5), p.475-477</ispartof><rights>2002 Urban & Fischer Verlag</rights><rights>COPYRIGHT 2002 Urban & Fischer Verlag</rights><rights>Copyright Urban & Fischer Verlag Jul 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-bb33c45c64db1fd724c256c0713ef97683d3aede8197c92c76ce3f3ebb6dba543</citedby><cites>FETCH-LOGICAL-c451t-bb33c45c64db1fd724c256c0713ef97683d3aede8197c92c76ce3f3ebb6dba543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/205524463?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994,64384,64386,64388,72240</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12222671$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gharagozloo, M.</creatorcontrib><creatorcontrib>Doroudchi, M.</creatorcontrib><creatorcontrib>Ghaderi, A.</creatorcontrib><title>Effects of Citrus aurantifolia concentrated extract on the spontaneous proliferation of MDA-MB-453 and RPMI-8866 tumor cell lines</title><title>Phytomedicine (Stuttgart)</title><addtitle>Phytomedicine</addtitle><description>The in vitro effects of concentrated lime juice (CLJ) extract on the spontaneous proliferation of a human breast carcinoma cell line (MDA-MB-453) and a human lymphoblastoid B cell line (RPMI-8866) were investigated. CLJ extract was prepared by freeze-drying fresh fruit juice and dialyzing the concentrated extract against phosphate buffered saline in order to deplete low molecular weight micronutrients such as flavonoids as well as adjusting the pH of the extract to the physiological range. The effects of different concentrations of the CLJ extract on the spontaneous proliferative responses of the cell lines were determined by
3H-thymidine incorporation after 24 hrs of incubation. CLJ extract had no significant effect on MDA-MB-453 cell line, however, using the concentrations of 125, 250, and 500 μg/ml of CLJ extract a significant inhibition of the spontaneous proliferation of RPMI-8866 cell line was detected (
P < 0.05). Due to the protein nature of the biologically active macromolecules of the CLJ extract (Gharagozloo and Ghaderi, 2001), it is reasonable to assume that the protein components of the CLJ extract may have anti-proliferative effects on tumor cell lines.</description><subject>Alternative medicine</subject><subject>antitumoraleffect</subject><subject>Breast cancer</subject><subject>Care and treatment</subject><subject>Cell Division - drug effects</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Citrus aurantifolia</subject><subject>Citrus aurantiifolia - chemistry</subject><subject>Evaluation</subject><subject>Health aspects</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>lime</subject><subject>Lime oil</subject><subject>MDA-MB-453</subject><subject>Medicinal plants</subject><subject>Plant Extracts - pharmacology</subject><subject>protein</subject><subject>RPMI-8866</subject><subject>Tumor Cells, Cultured</subject><issn>0944-7113</issn><issn>1618-095X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9ksGKFDEQhhtR3NnVB_AiwYMnW5NOd9KNp3FcdWEHRRS8hXRSWbP0JLNJWvS4b24NPSDoYnJIkfqq-FN_quoJoy8Zlf0rOrStZIw2gnaSyY7dq1ZMsL6mQ_ftfrU65GsE-El1mvM1pawdJH1YnbAGl5BsVd2eOwemZBId2fiS5kz0nHQo3sXJa2JiMBBK0gUsgZ8YmEJiIOU7kLyPoegAEYv2CXEHyHnMYrPt23W9fVO3HSc6WPL50_ai7nshSJl3MRED00QmHyA_qh44PWV4fDzPqq_vzr9sPtSXH99fbNaXtWk7Vupx5BwjI1o7Mmdl05qmE4ZKxsENUvTccg0WejZIMzRGCgPccRhHYUfdtfyser70Rak3M-Sidj4fZCwvULKhUgg2IPjsL_A6zimgNtXQrmvaVnCEXizQlZ5A-eDiYTRXEHAEUwzgPF6vByZQKO8Qr-_AcVvYeXMXzxbepJhzAqf2ye90-qUYVQfv1T_eY83To-553IH9U3E0G4HXCwA45h8eksrGA_prfcI_oGz0_2n_G-Ozusw</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>Gharagozloo, M.</creator><creator>Doroudchi, M.</creator><creator>Ghaderi, A.</creator><general>Elsevier GmbH</general><general>Urban & Fischer Verlag</general><general>Elsevier Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>20020701</creationdate><title>Effects of Citrus aurantifolia concentrated extract on the spontaneous proliferation of MDA-MB-453 and RPMI-8866 tumor cell lines</title><author>Gharagozloo, M. ; Doroudchi, M. ; Ghaderi, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-bb33c45c64db1fd724c256c0713ef97683d3aede8197c92c76ce3f3ebb6dba543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Alternative medicine</topic><topic>antitumoraleffect</topic><topic>Breast cancer</topic><topic>Care and treatment</topic><topic>Cell Division - drug effects</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Citrus aurantifolia</topic><topic>Citrus aurantiifolia - chemistry</topic><topic>Evaluation</topic><topic>Health aspects</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>lime</topic><topic>Lime oil</topic><topic>MDA-MB-453</topic><topic>Medicinal plants</topic><topic>Plant Extracts - pharmacology</topic><topic>protein</topic><topic>RPMI-8866</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gharagozloo, M.</creatorcontrib><creatorcontrib>Doroudchi, M.</creatorcontrib><creatorcontrib>Ghaderi, A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Phytomedicine (Stuttgart)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gharagozloo, M.</au><au>Doroudchi, M.</au><au>Ghaderi, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Citrus aurantifolia concentrated extract on the spontaneous proliferation of MDA-MB-453 and RPMI-8866 tumor cell lines</atitle><jtitle>Phytomedicine (Stuttgart)</jtitle><addtitle>Phytomedicine</addtitle><date>2002-07-01</date><risdate>2002</risdate><volume>9</volume><issue>5</issue><spage>475</spage><epage>477</epage><pages>475-477</pages><issn>0944-7113</issn><eissn>1618-095X</eissn><abstract>The in vitro effects of concentrated lime juice (CLJ) extract on the spontaneous proliferation of a human breast carcinoma cell line (MDA-MB-453) and a human lymphoblastoid B cell line (RPMI-8866) were investigated. CLJ extract was prepared by freeze-drying fresh fruit juice and dialyzing the concentrated extract against phosphate buffered saline in order to deplete low molecular weight micronutrients such as flavonoids as well as adjusting the pH of the extract to the physiological range. The effects of different concentrations of the CLJ extract on the spontaneous proliferative responses of the cell lines were determined by
3H-thymidine incorporation after 24 hrs of incubation. CLJ extract had no significant effect on MDA-MB-453 cell line, however, using the concentrations of 125, 250, and 500 μg/ml of CLJ extract a significant inhibition of the spontaneous proliferation of RPMI-8866 cell line was detected (
P < 0.05). Due to the protein nature of the biologically active macromolecules of the CLJ extract (Gharagozloo and Ghaderi, 2001), it is reasonable to assume that the protein components of the CLJ extract may have anti-proliferative effects on tumor cell lines.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>12222671</pmid><doi>10.1078/09447110260571751</doi><tpages>3</tpages></addata></record> |
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| identifier | ISSN: 0944-7113 |
| ispartof | Phytomedicine (Stuttgart), 2002-07, Vol.9 (5), p.475-477 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); ProQuest Central UK/Ireland |
| subjects | Alternative medicine antitumoraleffect Breast cancer Care and treatment Cell Division - drug effects Chromatography, High Pressure Liquid Citrus aurantifolia Citrus aurantiifolia - chemistry Evaluation Health aspects Humans In Vitro Techniques lime Lime oil MDA-MB-453 Medicinal plants Plant Extracts - pharmacology protein RPMI-8866 Tumor Cells, Cultured |
| title | Effects of Citrus aurantifolia concentrated extract on the spontaneous proliferation of MDA-MB-453 and RPMI-8866 tumor cell lines |
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