Comparison of two selective media for the detection and enumeration of Lactobacilli in human faeces
The enumeration of faecal bacteria is an important requirement for many studies of bowel health. One approach is the use of selective culture media for the culture and identification of genera or species from faeces. This study compares the culture of Lactobacilli from dilution series of faecal samp...
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| Veröffentlicht in: | Journal of microbiological methods 2002-11, Vol.51 (3), p.313-321 |
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| creator | Jackson, M.S Bird, A.R McOrist, A.L |
| description | The enumeration of faecal bacteria is an important requirement for many studies of bowel health. One approach is the use of selective culture media for the culture and identification of genera or species from faeces. This study compares the culture of
Lactobacilli from dilution series of faecal samples from six healthy human volunteers on two commonly used media, LAMVAB and Rogosa agar. Colonies were counted after a 72-h anaerobic incubation at 37 °C, and colony morphology recorded by a single observer. DNA was isolated from a representative number of colonies and genus-specific PCR, single-stranded conformation polymorphism (SSCP) and DNA sequencing performed. Total colony counts ranged from |
| doi_str_mv | 10.1016/S0167-7012(02)00102-1 |
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Lactobacilli from dilution series of faecal samples from six healthy human volunteers on two commonly used media, LAMVAB and Rogosa agar. Colonies were counted after a 72-h anaerobic incubation at 37 °C, and colony morphology recorded by a single observer. DNA was isolated from a representative number of colonies and genus-specific PCR, single-stranded conformation polymorphism (SSCP) and DNA sequencing performed. Total colony counts ranged from <3.00 to 7.48 log
10 cfu/g of faeces for LAMVAB and 5.09 to 7.66 log
10 cfu/g for Rogosa. For each subject, the total colony count was higher on Rogosa than that obtained with LAMVAB agar. SSCP analysis and DNA sequencing indicated that colony morphology was not an accurate predictor of genus identity. Growth of two species,
Lactobacillus acidophilus and
Lactobacillus gasseri, was not supported on LAMVAB medium. Rogosa agar was more likely to support growth of non-
Lactobacillus species. Therefore, neither medium gave a fully accurate representation of the
Lactobacilli species present in human faecal samples.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/S0167-7012(02)00102-1</identifier><identifier>PMID: 12223291</identifier><identifier>CODEN: JMIMDQ</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Adult ; Bacterial Typing Techniques ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; Colony Count, Microbial ; Culture Media ; DNA, Bacterial - analysis ; Faeces ; Feces - microbiology ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Lactobacilli ; Lactobacillus - classification ; Lactobacillus - genetics ; Lactobacillus - growth & development ; Lactobacillus - isolation & purification ; Media testing ; Microbiology ; Middle Aged ; PCR ; Polymerase Chain Reaction - methods ; Polymorphism, Single-Stranded Conformational ; Sequence Analysis, DNA</subject><ispartof>Journal of microbiological methods, 2002-11, Vol.51 (3), p.313-321</ispartof><rights>2002</rights><rights>2003 INIST-CNRS</rights><rights>Copyright 2002 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-e4c590b07f1ae590cd19f313fdb80281faa92b57bd61f9fe60bcfc420ec03a93</citedby><cites>FETCH-LOGICAL-c474t-e4c590b07f1ae590cd19f313fdb80281faa92b57bd61f9fe60bcfc420ec03a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0167-7012(02)00102-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13969111$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12223291$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jackson, M.S</creatorcontrib><creatorcontrib>Bird, A.R</creatorcontrib><creatorcontrib>McOrist, A.L</creatorcontrib><title>Comparison of two selective media for the detection and enumeration of Lactobacilli in human faeces</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>The enumeration of faecal bacteria is an important requirement for many studies of bowel health. One approach is the use of selective culture media for the culture and identification of genera or species from faeces. This study compares the culture of
Lactobacilli from dilution series of faecal samples from six healthy human volunteers on two commonly used media, LAMVAB and Rogosa agar. Colonies were counted after a 72-h anaerobic incubation at 37 °C, and colony morphology recorded by a single observer. DNA was isolated from a representative number of colonies and genus-specific PCR, single-stranded conformation polymorphism (SSCP) and DNA sequencing performed. Total colony counts ranged from <3.00 to 7.48 log
10 cfu/g of faeces for LAMVAB and 5.09 to 7.66 log
10 cfu/g for Rogosa. For each subject, the total colony count was higher on Rogosa than that obtained with LAMVAB agar. SSCP analysis and DNA sequencing indicated that colony morphology was not an accurate predictor of genus identity. Growth of two species,
Lactobacillus acidophilus and
Lactobacillus gasseri, was not supported on LAMVAB medium. Rogosa agar was more likely to support growth of non-
Lactobacillus species. Therefore, neither medium gave a fully accurate representation of the
Lactobacilli species present in human faecal samples.</description><subject>Adult</subject><subject>Bacterial Typing Techniques</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Colony Count, Microbial</subject><subject>Culture Media</subject><subject>DNA, Bacterial - analysis</subject><subject>Faeces</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Lactobacilli</subject><subject>Lactobacillus - classification</subject><subject>Lactobacillus - genetics</subject><subject>Lactobacillus - growth & development</subject><subject>Lactobacillus - isolation & purification</subject><subject>Media testing</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>PCR</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymorphism, Single-Stranded Conformational</subject><subject>Sequence Analysis, DNA</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EokvhJ4B8AcEhxWMn6_iE0Iq2SCtxaO_WxBmrRkm82ElR_z1Od0WPlSzbY31vPHqPsfcgLkDA9utN2XSlBcjPQn4RAoSs4AXbQKtl1arGvGSb_8gZe5Pz7wI1qm5fszOQUippYMPcLo4HTCHHiUfP57-RZxrIzeGe-Eh9QO5j4vMd8Z7m9b2AOPWcpmWkhI91Ee7RzbFDF4Yh8DDxu2XEiXskR_kte-VxyPTudJ6z28sft7vrav_r6ufu-75yta7nimrXGNEJ7QGp3FwPxitQvu9aIVvwiEZ2je76LXjjaSs6510tBTmh0Khz9unY9pDin4XybMeQHQ0DThSXbLUUWgmpngWhrY1qlS5gcwRdijkn8vaQwojpwYKwawr2MQW7WmxFWWsKForuw-mDpSsePqlOthfg4wnA7HDwCScX8hOnzNYArNy3I0fFtvtAyWYXaHIll1SysH0Mz4zyDwwrpHY</recordid><startdate>20021101</startdate><enddate>20021101</enddate><creator>Jackson, M.S</creator><creator>Bird, A.R</creator><creator>McOrist, A.L</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20021101</creationdate><title>Comparison of two selective media for the detection and enumeration of Lactobacilli in human faeces</title><author>Jackson, M.S ; Bird, A.R ; McOrist, A.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-e4c590b07f1ae590cd19f313fdb80281faa92b57bd61f9fe60bcfc420ec03a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adult</topic><topic>Bacterial Typing Techniques</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Colony Count, Microbial</topic><topic>Culture Media</topic><topic>DNA, Bacterial - analysis</topic><topic>Faeces</topic><topic>Feces - microbiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Lactobacilli</topic><topic>Lactobacillus - classification</topic><topic>Lactobacillus - genetics</topic><topic>Lactobacillus - growth & development</topic><topic>Lactobacillus - isolation & purification</topic><topic>Media testing</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>PCR</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymorphism, Single-Stranded Conformational</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jackson, M.S</creatorcontrib><creatorcontrib>Bird, A.R</creatorcontrib><creatorcontrib>McOrist, A.L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jackson, M.S</au><au>Bird, A.R</au><au>McOrist, A.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of two selective media for the detection and enumeration of Lactobacilli in human faeces</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2002-11-01</date><risdate>2002</risdate><volume>51</volume><issue>3</issue><spage>313</spage><epage>321</epage><pages>313-321</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><coden>JMIMDQ</coden><abstract>The enumeration of faecal bacteria is an important requirement for many studies of bowel health. One approach is the use of selective culture media for the culture and identification of genera or species from faeces. This study compares the culture of
Lactobacilli from dilution series of faecal samples from six healthy human volunteers on two commonly used media, LAMVAB and Rogosa agar. Colonies were counted after a 72-h anaerobic incubation at 37 °C, and colony morphology recorded by a single observer. DNA was isolated from a representative number of colonies and genus-specific PCR, single-stranded conformation polymorphism (SSCP) and DNA sequencing performed. Total colony counts ranged from <3.00 to 7.48 log
10 cfu/g of faeces for LAMVAB and 5.09 to 7.66 log
10 cfu/g for Rogosa. For each subject, the total colony count was higher on Rogosa than that obtained with LAMVAB agar. SSCP analysis and DNA sequencing indicated that colony morphology was not an accurate predictor of genus identity. Growth of two species,
Lactobacillus acidophilus and
Lactobacillus gasseri, was not supported on LAMVAB medium. Rogosa agar was more likely to support growth of non-
Lactobacillus species. Therefore, neither medium gave a fully accurate representation of the
Lactobacilli species present in human faecal samples.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>12223291</pmid><doi>10.1016/S0167-7012(02)00102-1</doi><tpages>9</tpages></addata></record> |
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| subjects | Adult Bacterial Typing Techniques Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Colony Count, Microbial Culture Media DNA, Bacterial - analysis Faeces Feces - microbiology Female Fundamental and applied biological sciences. Psychology Humans Lactobacilli Lactobacillus - classification Lactobacillus - genetics Lactobacillus - growth & development Lactobacillus - isolation & purification Media testing Microbiology Middle Aged PCR Polymerase Chain Reaction - methods Polymorphism, Single-Stranded Conformational Sequence Analysis, DNA |
| title | Comparison of two selective media for the detection and enumeration of Lactobacilli in human faeces |
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