Human immunodeficiency virus-1 protease. 2. Use of pH rate studies and solvent kinetic isotope effects to elucidate details of chemical mechanism

The pH dependence of the peptidolytic reaction of recombinant human immunodeficiency virus type 1 protease has been examined over a pH range of 3-7 for four oligopeptide substrates and two competitive inhibitors. The pK values obtained from the pKis vs pH profiles for the unprotonated and protonated...

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Veröffentlicht in:	Biochemistry (Easton) 1991-08, Vol.30 (34), p.8454-8463
Hauptverfasser:	Hyland, Lawrence J, Tomaszek, Thaddeus A, Meek, Thomas D
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Amino Acid Sequence Binding Sites Binding, Competitive Catalysis HIV Protease - chemistry Hydrogen-Ion Concentration Kinetics Molecular Sequence Data Oligopeptides - chemistry Oxygen Isotopes proteinase Solvents Substrate Specificity
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