Magnesium precipitate hot start method for PCR

For the amplification of HIV-1 gag gene, and other challenging targets, a simple new hot start PCR protocol is presented which consists simply and entirely of the buffer system. This novel buffer composition and reaction assembly protocol for PCR includes magnesium and phosphate combined at high con...

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Veröffentlicht in:	Molecular and cellular probes 2002-06, Vol.16 (3), p.167-171
Hauptverfasser:	Barnes, Wayne M, Rowlyk, Katherine R
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Buffers Cryptosporidium parvum - genetics DNA Primers DNA-Directed DNA Polymerase Gene Products, gag - genetics HIV-1 - genetics Hot Temperature Humans Magnesium PCR, hot start, primer dimer, magnesium precipitate, RockStart Phosphates Polymerase Chain Reaction - instrumentation Polymerase Chain Reaction - methods Tissue Plasminogen Activator - genetics
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