Novel approach for achieving double-layered cell sheets co-culture: overlaying endothelial cell sheets onto monolayer hepatocytes utilizing temperature-responsive culture dishes

Confluent human aortic endothelial cells (HAECs) cultured on thermo‐responsive culture dish grafted with poly (N‐isopropylacrylamide) were recovered as a contiguous cell sheet. The double‐layered co‐culture was achieved by placing the recovered HAEC sheet onto the rat hepatocyte layer directly. The...

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Veröffentlicht in:	Journal of biomedical materials research 2002-12, Vol.62 (3), p.464-470
Hauptverfasser:	Harimoto, Masami, Yamato, Masayuki, Hirose, Motohiro, Takahashi, Chie, Isoi, Yuki, Kikuchi, Akihiko, Okano, Teruo
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Coculture Techniques double-layered co-culture endothelial cell sheet Endothelium, Vascular - cytology Equipment and Supplies hepatocyte function Hepatocytes - cytology Humans Medical sciences Microscopy, Fluorescence Temperature thermo-responsive culture surface
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