Two-dimensional near-IR correlation spectroscopy study of molten globule-like state of ovalbumin in acidic pH region: Simultaneous changes in hydration and secondary structure

The molten globule‐like states of ovalbumin (OVA) in acid aqueous solutions are investigated by generalized two‐dimensional (2D) Fourier transform near‐IR (FT‐NIR) correlation spectroscopy. This new method allows us to explore the changes in hydration and the secondary structure simultaneously. FT‐N...

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Veröffentlicht in:	Biopolymers 2002, Vol.67 (6), p.394-405
Hauptverfasser:	Murayama, Koichi, Ozaki, Yukihiro
Format:	Artikel
Sprache:	eng
Schlagworte:	acidic pH hydration Hydrogen-Ion Concentration molten globule-like ovalbumin Ovalbumin - chemistry Protein Structure, Secondary secondary structure Solutions Spectrophotometry, Infrared - methods Spectroscopy, Fourier Transform Infrared - methods two-dimensional near-IR correlation spectroscopy
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