Regulation of fast inactivation of cloned mammalian Ik(A) channels by cysteine oxidation
Modulation of neuronal excitability by regulation of K+ channels potentially plays a part in short-term memory but has not yet been studied at the molecular level. Regulation of K+ channels by protein phosphorylation and oxygen has been described for various tissues and cell types; regulation of fas...
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| Veröffentlicht in: | Nature (London) 1991-08, Vol.352 (6337), p.711-714 |
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| container_issue | 6337 |
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| container_title | Nature (London) |
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| creator | RUPPERSBERG, J. P STOCKER, M PONGS, O HEINEMANN, S. H FRANK, R KOENEN, M |
| description | Modulation of neuronal excitability by regulation of K+ channels potentially plays a part in short-term memory but has not yet been studied at the molecular level. Regulation of K+ channels by protein phosphorylation and oxygen has been described for various tissues and cell types; regulation of fast-inactivating K+ channels mediating IK(A) currents has not yet been described. Functional expression of cloned mammalian K+ channels has provided a tool for studying their regulation at the molecular level. We report here that fast-inactivating K+ currents mediated by cloned K+ channel subunits derived from mammalian brain expressed in Xenopus oocytes are regulated by the reducing agent glutathione. This type of regulation may have a role in vivo to link metabolism to excitability and to regulate excitability in specific membrane areas of mammalian neurons. |
| doi_str_mv | 10.1038/352711a0 |
| format | Article |
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This type of regulation may have a role in vivo to link metabolism to excitability and to regulate excitability in specific membrane areas of mammalian neurons.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/352711a0</identifier><identifier>PMID: 1908562</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing</publisher><subject>Amino Acid Sequence ; Analytical, structural and metabolic biochemistry ; Animals ; Base Sequence ; Binding and carrier proteins ; Biological and medical sciences ; Cloning, Molecular ; Cysteine ; Drosophila melanogaster ; Fundamental and applied biological sciences. Psychology ; Glutathione - physiology ; In Vitro Techniques ; Ion Channel Gating ; Kinetics ; Molecular Sequence Data ; Oligonucleotides - chemistry ; Oocytes ; Oxidation-Reduction ; Potassium Channels - chemistry ; Proteins ; Recombinant Proteins ; Xenopus laevis</subject><ispartof>Nature (London), 1991-08, Vol.352 (6337), p.711-714</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-d8884ca782e36546e0dbd365f01c38ef33657b54e15b7a591754d00fa338ed363</citedby><cites>FETCH-LOGICAL-c373t-d8884ca782e36546e0dbd365f01c38ef33657b54e15b7a591754d00fa338ed363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4990504$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1908562$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>RUPPERSBERG, J. P</creatorcontrib><creatorcontrib>STOCKER, M</creatorcontrib><creatorcontrib>PONGS, O</creatorcontrib><creatorcontrib>HEINEMANN, S. H</creatorcontrib><creatorcontrib>FRANK, R</creatorcontrib><creatorcontrib>KOENEN, M</creatorcontrib><title>Regulation of fast inactivation of cloned mammalian Ik(A) channels by cysteine oxidation</title><title>Nature (London)</title><addtitle>Nature</addtitle><description>Modulation of neuronal excitability by regulation of K+ channels potentially plays a part in short-term memory but has not yet been studied at the molecular level. Regulation of K+ channels by protein phosphorylation and oxygen has been described for various tissues and cell types; regulation of fast-inactivating K+ channels mediating IK(A) currents has not yet been described. Functional expression of cloned mammalian K+ channels has provided a tool for studying their regulation at the molecular level. We report here that fast-inactivating K+ currents mediated by cloned K+ channel subunits derived from mammalian brain expressed in Xenopus oocytes are regulated by the reducing agent glutathione. This type of regulation may have a role in vivo to link metabolism to excitability and to regulate excitability in specific membrane areas of mammalian neurons.</description><subject>Amino Acid Sequence</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Binding and carrier proteins</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Cysteine</subject><subject>Drosophila melanogaster</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutathione - physiology</subject><subject>In Vitro Techniques</subject><subject>Ion Channel Gating</subject><subject>Kinetics</subject><subject>Molecular Sequence Data</subject><subject>Oligonucleotides - chemistry</subject><subject>Oocytes</subject><subject>Oxidation-Reduction</subject><subject>Potassium Channels - chemistry</subject><subject>Proteins</subject><subject>Recombinant Proteins</subject><subject>Xenopus laevis</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkF9LwzAUxYMoc07BLyDkQWQ-VG-apMkex_DPYCCIgm_lNk012qazacV9ezs359M93PO758Ih5JTBFQOur7mMFWMIe2TIhEoikWi1T4YAsY5A8-SQHIXwDgCSKTEgAzYBLZN4SF4e7WtXYutqT-uCFhha6jya1n3tlqasvc1phVWFpUNP5x_j6SU1b-i9LQPNVtSsQmudt7T-dvnv4TE5KLAM9mQ7R-T59uZpdh8tHu7ms-kiMlzxNsq11sKg0rHliRSJhTzLe1UAM1zbgvdaZVJYJjOFcsKUFDlAgbx3e5CPyMUmd9nUn50NbVq5YGxZord1F1IVgwSl1-B4A5qmDqGxRbpsXIXNKmWQrktM_0rs0bNtZpdVNv8HN631_vnWx2CwLBr0xoUdJiaT_qfgP9bld84</recordid><startdate>19910822</startdate><enddate>19910822</enddate><creator>RUPPERSBERG, J. 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Psychology</topic><topic>Glutathione - physiology</topic><topic>In Vitro Techniques</topic><topic>Ion Channel Gating</topic><topic>Kinetics</topic><topic>Molecular Sequence Data</topic><topic>Oligonucleotides - chemistry</topic><topic>Oocytes</topic><topic>Oxidation-Reduction</topic><topic>Potassium Channels - chemistry</topic><topic>Proteins</topic><topic>Recombinant Proteins</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RUPPERSBERG, J. P</creatorcontrib><creatorcontrib>STOCKER, M</creatorcontrib><creatorcontrib>PONGS, O</creatorcontrib><creatorcontrib>HEINEMANN, S. 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| ispartof | Nature (London), 1991-08, Vol.352 (6337), p.711-714 |
| issn | 0028-0836 1476-4687 |
| language | eng |
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| source | MEDLINE; Nature; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Analytical, structural and metabolic biochemistry Animals Base Sequence Binding and carrier proteins Biological and medical sciences Cloning, Molecular Cysteine Drosophila melanogaster Fundamental and applied biological sciences. Psychology Glutathione - physiology In Vitro Techniques Ion Channel Gating Kinetics Molecular Sequence Data Oligonucleotides - chemistry Oocytes Oxidation-Reduction Potassium Channels - chemistry Proteins Recombinant Proteins Xenopus laevis |
| title | Regulation of fast inactivation of cloned mammalian Ik(A) channels by cysteine oxidation |
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