First evidence of transmission of Leishmania ( Viannia) lainsoni in a Sub Andean region of Bolivia
Using ubiquitous primers which amplify the variable parts of kDNA minicircle of all Leishmania spp, we obtained for Leishmania (viannia) lainsoni a major band of 605 bp (band 1) shared with L. V. braziliensis and a minor 524 bp band (band 2) specific of L. V. lainsoni. The specificity of the two ban...
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description | Using ubiquitous primers which amplify the variable parts of kDNA minicircle of all
Leishmania spp, we obtained for
Leishmania (viannia) lainsoni a major band of 605 bp (band 1) shared with
L. V. braziliensis and a minor 524 bp band (band 2) specific of
L. V. lainsoni. The specificity of the two bands was examined through Southern blot hybridization of kDNA PCR obtained from reference strains belonging to
L. braziliensis,
L. mexicana,
L. donovani complexes with
L. V. lainsoni species. Band 1 was not specific of
L. V. lainsoni since it hybridized with some isolates belonging to
L. braziliensis complex. In contrast, band 2 was
L. V. lainsoni specific. PCR-based detection followed by hybridization with the new
L. V. lainsoni probe (Band 2) and
L. V. braziliensis probe (564 bp), was assayed using sample from a pool of 25 females of
Lutzomiya nuneztovari anglesi, blood, skin and liver samples of 18 mammals, spinal cords of four mammals and blood and cutaneous ulcers aspirates from 95 patents from Sub Andean region of La Paz, Bolivia. We observed a ositive hybridization of four patients lesions and the pool of
L. nuneztovari anglesi with the
L. V. lainsoni probe. It is the first time that
L. V. lainsoni is observed in a cycle of transmission in Bolivia. PCR products of three patients lesions and the pool of
L. nuneztovari anglesi were also hybridized with the specific probe of
L. V. braziliensis suggesting mixed infection in this focus. |
doi_str_mv | 10.1016/S0001-706X(02)00129-8 |
format | Article |
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Leishmania spp, we obtained for
Leishmania (viannia) lainsoni a major band of 605 bp (band 1) shared with
L. V. braziliensis and a minor 524 bp band (band 2) specific of
L. V. lainsoni. The specificity of the two bands was examined through Southern blot hybridization of kDNA PCR obtained from reference strains belonging to
L. braziliensis,
L. mexicana,
L. donovani complexes with
L. V. lainsoni species. Band 1 was not specific of
L. V. lainsoni since it hybridized with some isolates belonging to
L. braziliensis complex. In contrast, band 2 was
L. V. lainsoni specific. PCR-based detection followed by hybridization with the new
L. V. lainsoni probe (Band 2) and
L. V. braziliensis probe (564 bp), was assayed using sample from a pool of 25 females of
Lutzomiya nuneztovari anglesi, blood, skin and liver samples of 18 mammals, spinal cords of four mammals and blood and cutaneous ulcers aspirates from 95 patents from Sub Andean region of La Paz, Bolivia. We observed a ositive hybridization of four patients lesions and the pool of
L. nuneztovari anglesi with the
L. V. lainsoni probe. It is the first time that
L. V. lainsoni is observed in a cycle of transmission in Bolivia. PCR products of three patients lesions and the pool of
L. nuneztovari anglesi were also hybridized with the specific probe of
L. V. braziliensis suggesting mixed infection in this focus.</description><identifier>ISSN: 0001-706X</identifier><identifier>EISSN: 1873-6254</identifier><identifier>DOI: 10.1016/S0001-706X(02)00129-8</identifier><identifier>PMID: 12204398</identifier><identifier>CODEN: ACTRAQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Bolivia ; DNA, Kinetoplast - isolation & purification ; Human protozoal diseases ; Humans ; Hybridization, Genetic ; Infectious diseases ; Kinetoplast ; Leishmania ( Viannia) lainsoni ; Leishmania - isolation & purification ; Leishmaniasis - epidemiology ; Leishmaniasis - transmission ; Leshmaniasis ; Medical sciences ; Parasitic diseases ; PCR-based diagnosis ; Polymerase Chain Reaction ; Protozoal diseases ; Specific probe ; Transmission ; Tropical medicine</subject><ispartof>Acta tropica, 2002-09, Vol.83 (3), p.249-253</ispartof><rights>2002 Elsevier Science B.V.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c519t-475ab43c2a2d3d163a7d1d2e5691c02c984d104c1db54b136dc4e49cb6569b013</citedby><cites>FETCH-LOGICAL-c519t-475ab43c2a2d3d163a7d1d2e5691c02c984d104c1db54b136dc4e49cb6569b013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0001-706X(02)00129-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13863885$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12204398$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bastrenta, B</creatorcontrib><creatorcontrib>Buitrago, R</creatorcontrib><creatorcontrib>Vargas, F</creatorcontrib><creatorcontrib>Le Pont, F</creatorcontrib><creatorcontrib>Torrez, M</creatorcontrib><creatorcontrib>Flores, M</creatorcontrib><creatorcontrib>Mita, N</creatorcontrib><creatorcontrib>Brenière, S.F</creatorcontrib><title>First evidence of transmission of Leishmania ( Viannia) lainsoni in a Sub Andean region of Bolivia</title><title>Acta tropica</title><addtitle>Acta Trop</addtitle><description>Using ubiquitous primers which amplify the variable parts of kDNA minicircle of all
Leishmania spp, we obtained for
Leishmania (viannia) lainsoni a major band of 605 bp (band 1) shared with
L. V. braziliensis and a minor 524 bp band (band 2) specific of
L. V. lainsoni. The specificity of the two bands was examined through Southern blot hybridization of kDNA PCR obtained from reference strains belonging to
L. braziliensis,
L. mexicana,
L. donovani complexes with
L. V. lainsoni species. Band 1 was not specific of
L. V. lainsoni since it hybridized with some isolates belonging to
L. braziliensis complex. In contrast, band 2 was
L. V. lainsoni specific. PCR-based detection followed by hybridization with the new
L. V. lainsoni probe (Band 2) and
L. V. braziliensis probe (564 bp), was assayed using sample from a pool of 25 females of
Lutzomiya nuneztovari anglesi, blood, skin and liver samples of 18 mammals, spinal cords of four mammals and blood and cutaneous ulcers aspirates from 95 patents from Sub Andean region of La Paz, Bolivia. We observed a ositive hybridization of four patients lesions and the pool of
L. nuneztovari anglesi with the
L. V. lainsoni probe. It is the first time that
L. V. lainsoni is observed in a cycle of transmission in Bolivia. PCR products of three patients lesions and the pool of
L. nuneztovari anglesi were also hybridized with the specific probe of
L. V. braziliensis suggesting mixed infection in this focus.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bolivia</subject><subject>DNA, Kinetoplast - isolation & purification</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Hybridization, Genetic</subject><subject>Infectious diseases</subject><subject>Kinetoplast</subject><subject>Leishmania ( Viannia) lainsoni</subject><subject>Leishmania - isolation & purification</subject><subject>Leishmaniasis - epidemiology</subject><subject>Leishmaniasis - transmission</subject><subject>Leshmaniasis</subject><subject>Medical sciences</subject><subject>Parasitic diseases</subject><subject>PCR-based diagnosis</subject><subject>Polymerase Chain Reaction</subject><subject>Protozoal diseases</subject><subject>Specific probe</subject><subject>Transmission</subject><subject>Tropical medicine</subject><issn>0001-706X</issn><issn>1873-6254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi1ERZfCTwD5AmoPKf6Oc0KlolBppR4KiJvl2LMwKOu0dnYl_j1ON6LHnmZGeubrfQl5w9k5Z9x8uGWM8aZl5ucpE2c1F11jn5EVt61sjNDqOVn9R47Jy1L-zFCrxQtyzIVgSnZ2RforzGWisMcIKQAdN3TKPpUtloJjmus1YPm99Qk9PaU_0KeandHBYypjQoqJenq76-lFiuATzfBrafw0DrhH_4ocbfxQ4PUST8j3q8_fLr8265sv15cX6yZo3k2NarXvlQzCiygjN9K3kUcB2nQ8MBE6qyJnKvDYa9VzaWJQoLrQm0r0jMsT8v4w9y6P9zsok6tPBBgGn2DcFdfWn7vW2idBKaTR3JgK6gMY8lhKho27y7j1-a_jzM0uuAcX3CyxY8I9uODmBW-XBbt-C_Gxa5G9Au8WwJfgh01VPGB55KQ10lpduY8HDqpue4TsSsDZp4gZwuTiiE-c8g9L_aLE</recordid><startdate>20020901</startdate><enddate>20020901</enddate><creator>Bastrenta, B</creator><creator>Buitrago, R</creator><creator>Vargas, F</creator><creator>Le Pont, F</creator><creator>Torrez, M</creator><creator>Flores, M</creator><creator>Mita, N</creator><creator>Brenière, S.F</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20020901</creationdate><title>First evidence of transmission of Leishmania ( Viannia) lainsoni in a Sub Andean region of Bolivia</title><author>Bastrenta, B ; Buitrago, R ; Vargas, F ; Le Pont, F ; Torrez, M ; Flores, M ; Mita, N ; Brenière, S.F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c519t-475ab43c2a2d3d163a7d1d2e5691c02c984d104c1db54b136dc4e49cb6569b013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bolivia</topic><topic>DNA, Kinetoplast - isolation & purification</topic><topic>Human protozoal diseases</topic><topic>Humans</topic><topic>Hybridization, Genetic</topic><topic>Infectious diseases</topic><topic>Kinetoplast</topic><topic>Leishmania ( Viannia) lainsoni</topic><topic>Leishmania - isolation & purification</topic><topic>Leishmaniasis - epidemiology</topic><topic>Leishmaniasis - transmission</topic><topic>Leshmaniasis</topic><topic>Medical sciences</topic><topic>Parasitic diseases</topic><topic>PCR-based diagnosis</topic><topic>Polymerase Chain Reaction</topic><topic>Protozoal diseases</topic><topic>Specific probe</topic><topic>Transmission</topic><topic>Tropical medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bastrenta, B</creatorcontrib><creatorcontrib>Buitrago, R</creatorcontrib><creatorcontrib>Vargas, F</creatorcontrib><creatorcontrib>Le Pont, F</creatorcontrib><creatorcontrib>Torrez, M</creatorcontrib><creatorcontrib>Flores, M</creatorcontrib><creatorcontrib>Mita, N</creatorcontrib><creatorcontrib>Brenière, S.F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Acta tropica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bastrenta, B</au><au>Buitrago, R</au><au>Vargas, F</au><au>Le Pont, F</au><au>Torrez, M</au><au>Flores, M</au><au>Mita, N</au><au>Brenière, S.F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First evidence of transmission of Leishmania ( Viannia) lainsoni in a Sub Andean region of Bolivia</atitle><jtitle>Acta tropica</jtitle><addtitle>Acta Trop</addtitle><date>2002-09-01</date><risdate>2002</risdate><volume>83</volume><issue>3</issue><spage>249</spage><epage>253</epage><pages>249-253</pages><issn>0001-706X</issn><eissn>1873-6254</eissn><coden>ACTRAQ</coden><abstract>Using ubiquitous primers which amplify the variable parts of kDNA minicircle of all
Leishmania spp, we obtained for
Leishmania (viannia) lainsoni a major band of 605 bp (band 1) shared with
L. V. braziliensis and a minor 524 bp band (band 2) specific of
L. V. lainsoni. The specificity of the two bands was examined through Southern blot hybridization of kDNA PCR obtained from reference strains belonging to
L. braziliensis,
L. mexicana,
L. donovani complexes with
L. V. lainsoni species. Band 1 was not specific of
L. V. lainsoni since it hybridized with some isolates belonging to
L. braziliensis complex. In contrast, band 2 was
L. V. lainsoni specific. PCR-based detection followed by hybridization with the new
L. V. lainsoni probe (Band 2) and
L. V. braziliensis probe (564 bp), was assayed using sample from a pool of 25 females of
Lutzomiya nuneztovari anglesi, blood, skin and liver samples of 18 mammals, spinal cords of four mammals and blood and cutaneous ulcers aspirates from 95 patents from Sub Andean region of La Paz, Bolivia. We observed a ositive hybridization of four patients lesions and the pool of
L. nuneztovari anglesi with the
L. V. lainsoni probe. It is the first time that
L. V. lainsoni is observed in a cycle of transmission in Bolivia. PCR products of three patients lesions and the pool of
L. nuneztovari anglesi were also hybridized with the specific probe of
L. V. braziliensis suggesting mixed infection in this focus.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>12204398</pmid><doi>10.1016/S0001-706X(02)00129-8</doi><tpages>5</tpages></addata></record> |
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subjects | Animals Biological and medical sciences Bolivia DNA, Kinetoplast - isolation & purification Human protozoal diseases Humans Hybridization, Genetic Infectious diseases Kinetoplast Leishmania ( Viannia) lainsoni Leishmania - isolation & purification Leishmaniasis - epidemiology Leishmaniasis - transmission Leshmaniasis Medical sciences Parasitic diseases PCR-based diagnosis Polymerase Chain Reaction Protozoal diseases Specific probe Transmission Tropical medicine |
title | First evidence of transmission of Leishmania ( Viannia) lainsoni in a Sub Andean region of Bolivia |
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