Small acidic ;peptides are bound to E. coli DNA
Low molecular weight peptides have been isolated by alkali extraction from deproteinized DNA of E. coli cells grown in the presence of radioactive glutamic acid or orthophosphate. The labeled peptides, purified by gel filtration chromatography on Sephadex G25 and G10, contain prevailingly glutamic a...
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| Veröffentlicht in: | Molecular biology reports 1991-02, Vol.15 (1), p.9-18 |
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| container_title | Molecular biology reports |
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| creator | FELICI, F CARDELLINI, E MIANO, A BRAMUCCI, M PICCININI, G AMICI, D GIANFRANCESCHI, G. L |
| description | Low molecular weight peptides have been isolated by alkali extraction from deproteinized DNA of E. coli cells grown in the presence of radioactive glutamic acid or orthophosphate. The labeled peptides, purified by gel filtration chromatography on Sephadex G25 and G10, contain prevailingly glutamic acid, aspartic acid, glycine, serine and alanine. Electrophoretic studies at different pH show that some peptide fractions contain a phosphoric residue. The N-terminus of the phosphorylated peptides is apparently blocked and they were able to bind to DNA in the presence of Mg2+ ions. Moreover the acidic peptides extracted from E. coli DNA show a sharp activity in the control of lambda phage DNA transcription 'in vitro'. |
| doi_str_mv | 10.1007/BF00369895 |
| format | Article |
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L</creatorcontrib><title>Small acidic ;peptides are bound to E. coli DNA</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><description>Low molecular weight peptides have been isolated by alkali extraction from deproteinized DNA of E. coli cells grown in the presence of radioactive glutamic acid or orthophosphate. The labeled peptides, purified by gel filtration chromatography on Sephadex G25 and G10, contain prevailingly glutamic acid, aspartic acid, glycine, serine and alanine. Electrophoretic studies at different pH show that some peptide fractions contain a phosphoric residue. The N-terminus of the phosphorylated peptides is apparently blocked and they were able to bind to DNA in the presence of Mg2+ ions. Moreover the acidic peptides extracted from E. coli DNA show a sharp activity in the control of lambda phage DNA transcription 'in vitro'.</description><subject>Amino Acids - analysis</subject><subject>Aminoacids, peptides. Hormones. Neuropeptides</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatin - chemistry</subject><subject>Chromatography, Gel</subject><subject>Chromatography, Thin Layer</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>DNA-Binding Proteins - isolation & purification</subject><subject>Electrophoresis</subject><subject>Escherichia coli</subject><subject>Escherichia coli - physiology</subject><subject>Fundamental and applied biological sciences. 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| fulltext | fulltext |
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| ispartof | Molecular biology reports, 1991-02, Vol.15 (1), p.9-18 |
| issn | 0301-4851 1573-4978 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Amino Acids - analysis Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatin - chemistry Chromatography, Gel Chromatography, Thin Layer DNA, Bacterial - isolation & purification DNA-Binding Proteins - isolation & purification Electrophoresis Escherichia coli Escherichia coli - physiology Fundamental and applied biological sciences. Psychology Molecular Weight Peptides - isolation & purification Phosphopeptides - analysis Proteins Transcription, Genetic - physiology |
| title | Small acidic ;peptides are bound to E. coli DNA |
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