Administration of proteolytic enzymes bromelain and trypsin diminish the number of CD4+ cells and the interferon-γ response in Peyer's patches and spleen in endotoxemic balb/c mice
Recent publications revealed that bromelain exerts a marked effect on T-cell response by inhibiting T-cell signal transduction. These experimental studies may help to explain former clinical investigations showing that Phlogenzym (PHL), a preparation consisting of the proteases bromelain and trypsin...
Gespeichert in:
| Veröffentlicht in: | Cellular immunology 2002-02, Vol.215 (2), p.113-119 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 119 |
|---|---|
| container_issue | 2 |
| container_start_page | 113 |
| container_title | Cellular immunology |
| container_volume | 215 |
| creator | Manhart, N Akomeah, R Bergmeister, H Spittler, A Ploner, M Roth, E |
| description | Recent publications revealed that bromelain exerts a marked effect on T-cell response by inhibiting T-cell signal transduction. These experimental studies may help to explain former clinical investigations showing that Phlogenzym (PHL), a preparation consisting of the proteases bromelain and trypsin and the antioxidant rutosid, ameliorate certain diseases with an underlying inflammatory process. In this study, we showed that orally administered PHL significantly reduced lymphocyte subpopulations in Peyer's patches (PPs) of healthy and endotoxemic mice. Similarly, the number of splenic lymphocytes in endotoxin-boostered mice was significantly lowered by PHL. The effect of PHL was more pronounced on T cells than on B cells leading especially to a diminution of CD4+ cells. Moreover, PHL pretreatment decreased IFN-γ mRNA in PPs and spleen of endotoxemic mice. These results reveal that PHL may ameliorate inflammatory process by reducing the number of CD4+ cells and by diminishing INF-γ mRNA levels. |
| doi_str_mv | 10.1016/S0008-8749(02)00019-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72045706</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0008874902000199</els_id><sourcerecordid>18472969</sourcerecordid><originalsourceid>FETCH-LOGICAL-c392t-bc13b06d44e72cc0bf1383f7a770d0528746dfd36dfafee3a0975a1fc37afb063</originalsourceid><addsrcrecordid>eNqNkd-O1CAUxonRuOPqI2i40jWmLtBOKVdmM_5NNtFEvSYUDhlMCxUYY_e1jK_hM0mnE73UGzhwft85cD6EHlLynBLaXn4khHRVxxtxQdjTcqCiErfQhhJBKkbb-jba_EHO0L2UvhSGNoLcRWeUMcJo023QzyszOu9Sjiq74HGweIohQxjm7DQGfzOPkHAfwwiDch4rb3CO85RKbNxRu8d5D9gfxh7iUmD3snmGNQxDWumSdD5DtBCDr379wBHSFHxarvEHmCE-SXhSWe9hVaRpAPBLFrwJOXyHsbylV0N_qXEJ4T66Y9WQ4MFpP0efX7_6tHtbXb9_8253dV3pWrBc9ZrWPWlN0wBnWpPe0rqrLVecE0O2rIymNdbUZVEWoFZE8K2iVtdc2SKsz9HjtW6ZydcDpCxHl5afKQ_hkCRnpNny_wBp13AmWlHA7QrqGFKKYOUU3ajiLCmRi7HyaKxcXJOEyaOxctE9OjU49COYv6qTkwV4sQJQ5vHNQZRJO_AajIugszTB_aPFbxGbt0Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18472969</pqid></control><display><type>article</type><title>Administration of proteolytic enzymes bromelain and trypsin diminish the number of CD4+ cells and the interferon-γ response in Peyer's patches and spleen in endotoxemic balb/c mice</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Manhart, N ; Akomeah, R ; Bergmeister, H ; Spittler, A ; Ploner, M ; Roth, E</creator><creatorcontrib>Manhart, N ; Akomeah, R ; Bergmeister, H ; Spittler, A ; Ploner, M ; Roth, E</creatorcontrib><description>Recent publications revealed that bromelain exerts a marked effect on T-cell response by inhibiting T-cell signal transduction. These experimental studies may help to explain former clinical investigations showing that Phlogenzym (PHL), a preparation consisting of the proteases bromelain and trypsin and the antioxidant rutosid, ameliorate certain diseases with an underlying inflammatory process. In this study, we showed that orally administered PHL significantly reduced lymphocyte subpopulations in Peyer's patches (PPs) of healthy and endotoxemic mice. Similarly, the number of splenic lymphocytes in endotoxin-boostered mice was significantly lowered by PHL. The effect of PHL was more pronounced on T cells than on B cells leading especially to a diminution of CD4+ cells. Moreover, PHL pretreatment decreased IFN-γ mRNA in PPs and spleen of endotoxemic mice. These results reveal that PHL may ameliorate inflammatory process by reducing the number of CD4+ cells and by diminishing INF-γ mRNA levels.</description><identifier>ISSN: 0008-8749</identifier><identifier>EISSN: 1090-2163</identifier><identifier>DOI: 10.1016/S0008-8749(02)00019-9</identifier><identifier>PMID: 12202148</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Anti-Inflammatory Agents, Non-Steroidal - pharmacology ; Bromelains - administration & dosage ; Bromelains - pharmacology ; CD4-Positive T-Lymphocytes - drug effects ; CD4-Positive T-Lymphocytes - immunology ; CD4-Positive T-Lymphocytes - metabolism ; Cytokines - genetics ; Cytokines - metabolism ; Drug Combinations ; Endotoxemia - immunology ; Enzyme therapy ; Female ; Interferon-gamma - metabolism ; Intestinal immunology ; Mice ; Mice, Inbred BALB C ; Mucosa immunology ; Peyer's Patches - cytology ; Peyer's Patches - immunology ; Peyer's Patches - metabolism ; Proteases ; RNA, Messenger - metabolism ; Rutin - administration & dosage ; Rutin - analogs & derivatives ; Rutin - pharmacology ; Spleen - cytology ; Spleen - immunology ; Spleen - metabolism ; Trypsin - administration & dosage ; Trypsin - pharmacology</subject><ispartof>Cellular immunology, 2002-02, Vol.215 (2), p.113-119</ispartof><rights>2002 Elsevier Science (USA)</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-bc13b06d44e72cc0bf1383f7a770d0528746dfd36dfafee3a0975a1fc37afb063</citedby><cites>FETCH-LOGICAL-c392t-bc13b06d44e72cc0bf1383f7a770d0528746dfd36dfafee3a0975a1fc37afb063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0008874902000199$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12202148$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Manhart, N</creatorcontrib><creatorcontrib>Akomeah, R</creatorcontrib><creatorcontrib>Bergmeister, H</creatorcontrib><creatorcontrib>Spittler, A</creatorcontrib><creatorcontrib>Ploner, M</creatorcontrib><creatorcontrib>Roth, E</creatorcontrib><title>Administration of proteolytic enzymes bromelain and trypsin diminish the number of CD4+ cells and the interferon-γ response in Peyer's patches and spleen in endotoxemic balb/c mice</title><title>Cellular immunology</title><addtitle>Cell Immunol</addtitle><description>Recent publications revealed that bromelain exerts a marked effect on T-cell response by inhibiting T-cell signal transduction. These experimental studies may help to explain former clinical investigations showing that Phlogenzym (PHL), a preparation consisting of the proteases bromelain and trypsin and the antioxidant rutosid, ameliorate certain diseases with an underlying inflammatory process. In this study, we showed that orally administered PHL significantly reduced lymphocyte subpopulations in Peyer's patches (PPs) of healthy and endotoxemic mice. Similarly, the number of splenic lymphocytes in endotoxin-boostered mice was significantly lowered by PHL. The effect of PHL was more pronounced on T cells than on B cells leading especially to a diminution of CD4+ cells. Moreover, PHL pretreatment decreased IFN-γ mRNA in PPs and spleen of endotoxemic mice. These results reveal that PHL may ameliorate inflammatory process by reducing the number of CD4+ cells and by diminishing INF-γ mRNA levels.</description><subject>Animals</subject><subject>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</subject><subject>Bromelains - administration & dosage</subject><subject>Bromelains - pharmacology</subject><subject>CD4-Positive T-Lymphocytes - drug effects</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD4-Positive T-Lymphocytes - metabolism</subject><subject>Cytokines - genetics</subject><subject>Cytokines - metabolism</subject><subject>Drug Combinations</subject><subject>Endotoxemia - immunology</subject><subject>Enzyme therapy</subject><subject>Female</subject><subject>Interferon-gamma - metabolism</subject><subject>Intestinal immunology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mucosa immunology</subject><subject>Peyer's Patches - cytology</subject><subject>Peyer's Patches - immunology</subject><subject>Peyer's Patches - metabolism</subject><subject>Proteases</subject><subject>RNA, Messenger - metabolism</subject><subject>Rutin - administration & dosage</subject><subject>Rutin - analogs & derivatives</subject><subject>Rutin - pharmacology</subject><subject>Spleen - cytology</subject><subject>Spleen - immunology</subject><subject>Spleen - metabolism</subject><subject>Trypsin - administration & dosage</subject><subject>Trypsin - pharmacology</subject><issn>0008-8749</issn><issn>1090-2163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkd-O1CAUxonRuOPqI2i40jWmLtBOKVdmM_5NNtFEvSYUDhlMCxUYY_e1jK_hM0mnE73UGzhwft85cD6EHlLynBLaXn4khHRVxxtxQdjTcqCiErfQhhJBKkbb-jba_EHO0L2UvhSGNoLcRWeUMcJo023QzyszOu9Sjiq74HGweIohQxjm7DQGfzOPkHAfwwiDch4rb3CO85RKbNxRu8d5D9gfxh7iUmD3snmGNQxDWumSdD5DtBCDr379wBHSFHxarvEHmCE-SXhSWe9hVaRpAPBLFrwJOXyHsbylV0N_qXEJ4T66Y9WQ4MFpP0efX7_6tHtbXb9_8253dV3pWrBc9ZrWPWlN0wBnWpPe0rqrLVecE0O2rIymNdbUZVEWoFZE8K2iVtdc2SKsz9HjtW6ZydcDpCxHl5afKQ_hkCRnpNny_wBp13AmWlHA7QrqGFKKYOUU3ajiLCmRi7HyaKxcXJOEyaOxctE9OjU49COYv6qTkwV4sQJQ5vHNQZRJO_AajIugszTB_aPFbxGbt0Q</recordid><startdate>20020201</startdate><enddate>20020201</enddate><creator>Manhart, N</creator><creator>Akomeah, R</creator><creator>Bergmeister, H</creator><creator>Spittler, A</creator><creator>Ploner, M</creator><creator>Roth, E</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20020201</creationdate><title>Administration of proteolytic enzymes bromelain and trypsin diminish the number of CD4+ cells and the interferon-γ response in Peyer's patches and spleen in endotoxemic balb/c mice</title><author>Manhart, N ; Akomeah, R ; Bergmeister, H ; Spittler, A ; Ploner, M ; Roth, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-bc13b06d44e72cc0bf1383f7a770d0528746dfd36dfafee3a0975a1fc37afb063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Anti-Inflammatory Agents, Non-Steroidal - pharmacology</topic><topic>Bromelains - administration & dosage</topic><topic>Bromelains - pharmacology</topic><topic>CD4-Positive T-Lymphocytes - drug effects</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>CD4-Positive T-Lymphocytes - metabolism</topic><topic>Cytokines - genetics</topic><topic>Cytokines - metabolism</topic><topic>Drug Combinations</topic><topic>Endotoxemia - immunology</topic><topic>Enzyme therapy</topic><topic>Female</topic><topic>Interferon-gamma - metabolism</topic><topic>Intestinal immunology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mucosa immunology</topic><topic>Peyer's Patches - cytology</topic><topic>Peyer's Patches - immunology</topic><topic>Peyer's Patches - metabolism</topic><topic>Proteases</topic><topic>RNA, Messenger - metabolism</topic><topic>Rutin - administration & dosage</topic><topic>Rutin - analogs & derivatives</topic><topic>Rutin - pharmacology</topic><topic>Spleen - cytology</topic><topic>Spleen - immunology</topic><topic>Spleen - metabolism</topic><topic>Trypsin - administration & dosage</topic><topic>Trypsin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Manhart, N</creatorcontrib><creatorcontrib>Akomeah, R</creatorcontrib><creatorcontrib>Bergmeister, H</creatorcontrib><creatorcontrib>Spittler, A</creatorcontrib><creatorcontrib>Ploner, M</creatorcontrib><creatorcontrib>Roth, E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Manhart, N</au><au>Akomeah, R</au><au>Bergmeister, H</au><au>Spittler, A</au><au>Ploner, M</au><au>Roth, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Administration of proteolytic enzymes bromelain and trypsin diminish the number of CD4+ cells and the interferon-γ response in Peyer's patches and spleen in endotoxemic balb/c mice</atitle><jtitle>Cellular immunology</jtitle><addtitle>Cell Immunol</addtitle><date>2002-02-01</date><risdate>2002</risdate><volume>215</volume><issue>2</issue><spage>113</spage><epage>119</epage><pages>113-119</pages><issn>0008-8749</issn><eissn>1090-2163</eissn><abstract>Recent publications revealed that bromelain exerts a marked effect on T-cell response by inhibiting T-cell signal transduction. These experimental studies may help to explain former clinical investigations showing that Phlogenzym (PHL), a preparation consisting of the proteases bromelain and trypsin and the antioxidant rutosid, ameliorate certain diseases with an underlying inflammatory process. In this study, we showed that orally administered PHL significantly reduced lymphocyte subpopulations in Peyer's patches (PPs) of healthy and endotoxemic mice. Similarly, the number of splenic lymphocytes in endotoxin-boostered mice was significantly lowered by PHL. The effect of PHL was more pronounced on T cells than on B cells leading especially to a diminution of CD4+ cells. Moreover, PHL pretreatment decreased IFN-γ mRNA in PPs and spleen of endotoxemic mice. These results reveal that PHL may ameliorate inflammatory process by reducing the number of CD4+ cells and by diminishing INF-γ mRNA levels.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>12202148</pmid><doi>10.1016/S0008-8749(02)00019-9</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0008-8749 |
| ispartof | Cellular immunology, 2002-02, Vol.215 (2), p.113-119 |
| issn | 0008-8749 1090-2163 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72045706 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Anti-Inflammatory Agents, Non-Steroidal - pharmacology Bromelains - administration & dosage Bromelains - pharmacology CD4-Positive T-Lymphocytes - drug effects CD4-Positive T-Lymphocytes - immunology CD4-Positive T-Lymphocytes - metabolism Cytokines - genetics Cytokines - metabolism Drug Combinations Endotoxemia - immunology Enzyme therapy Female Interferon-gamma - metabolism Intestinal immunology Mice Mice, Inbred BALB C Mucosa immunology Peyer's Patches - cytology Peyer's Patches - immunology Peyer's Patches - metabolism Proteases RNA, Messenger - metabolism Rutin - administration & dosage Rutin - analogs & derivatives Rutin - pharmacology Spleen - cytology Spleen - immunology Spleen - metabolism Trypsin - administration & dosage Trypsin - pharmacology |
| title | Administration of proteolytic enzymes bromelain and trypsin diminish the number of CD4+ cells and the interferon-γ response in Peyer's patches and spleen in endotoxemic balb/c mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T13%3A00%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Administration%20of%20proteolytic%20enzymes%20bromelain%20and%20trypsin%20diminish%20the%20number%20of%20CD4+%20cells%20and%20the%20interferon-%CE%B3%20response%20in%20Peyer's%20patches%20and%20spleen%20in%20endotoxemic%20balb/c%20mice&rft.jtitle=Cellular%20immunology&rft.au=Manhart,%20N&rft.date=2002-02-01&rft.volume=215&rft.issue=2&rft.spage=113&rft.epage=119&rft.pages=113-119&rft.issn=0008-8749&rft.eissn=1090-2163&rft_id=info:doi/10.1016/S0008-8749(02)00019-9&rft_dat=%3Cproquest_cross%3E18472969%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18472969&rft_id=info:pmid/12202148&rft_els_id=S0008874902000199&rfr_iscdi=true |