Detection of brain-derived neurotrophic factor-like activity in fibroblasts and Schwann cells: Inhibition by antibodies to NGF
mRNA coding for brain-derived neurotrophic factor (BDNF) has been detected in cultured L929 fibroblasts, rat dermal fibroblasts, and sciatic nerve Schwann cells, as well as in rat skin. Medium conditioned by cultured fibroblasts and Schwann cells also stimulates neurite growth from retinal explants...
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| Veröffentlicht in: | Neuron (Cambridge, Mass.) Mass.), 1991-08, Vol.7 (2), p.265-275 |
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| container_title | Neuron (Cambridge, Mass.) |
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| creator | Acheson, Ann Barker, Philip A. Alderson, Ralph F. Miller, Freda D. Murphy, Richard A. |
| description | mRNA coding for brain-derived neurotrophic factor (BDNF) has been detected in cultured L929 fibroblasts, rat dermal fibroblasts, and sciatic nerve Schwann cells, as well as in rat skin. Medium conditioned by cultured fibroblasts and Schwann cells also stimulates neurite growth from retinal explants and promotes the survival in culture of BDNF-responsive sensory neurons; biological activity is abolished by antibodies raised against NGF. These results suggest that molecules with BDNF-like activity may be produced by cells in the peripheral nervous system and that the BDNF-like activity in fibroblasts and Schwann cells is derived from molecules immunologically related to NGF. In support of this concept, antibodies against NGF have been found to reduce the biological activity of recombinant BDNF in culture and to cross-react with BDNF on Western blots. |
| doi_str_mv | 10.1016/0896-6273(91)90265-2 |
| format | Article |
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Medium conditioned by cultured fibroblasts and Schwann cells also stimulates neurite growth from retinal explants and promotes the survival in culture of BDNF-responsive sensory neurons; biological activity is abolished by antibodies raised against NGF. These results suggest that molecules with BDNF-like activity may be produced by cells in the peripheral nervous system and that the BDNF-like activity in fibroblasts and Schwann cells is derived from molecules immunologically related to NGF. In support of this concept, antibodies against NGF have been found to reduce the biological activity of recombinant BDNF in culture and to cross-react with BDNF on Western blots.</description><identifier>ISSN: 0896-6273</identifier><identifier>EISSN: 1097-4199</identifier><identifier>DOI: 10.1016/0896-6273(91)90265-2</identifier><identifier>PMID: 1873030</identifier><identifier>CODEN: NERNET</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>Animals ; Antibodies - immunology ; Antibodies - pharmacology ; Axons - drug effects ; Axons - physiology ; Biological and medical sciences ; Blotting, Western ; Brain - metabolism ; Brain-Derived Neurotrophic Factor ; Cell Survival - drug effects ; Cells, Cultured ; Chick Embryo ; Culture Media - pharmacology ; Fibroblasts - metabolism ; Fundamental and applied biological sciences. Psychology ; Metabolisms and neurohumoral controls ; Mice ; Nerve Growth Factors - immunology ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - immunology ; Nerve Tissue Proteins - metabolism ; Neurons, Afferent - drug effects ; Nitrogen metabolism. Proteins. Glycoproteins. Nucleic acids. 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Medium conditioned by cultured fibroblasts and Schwann cells also stimulates neurite growth from retinal explants and promotes the survival in culture of BDNF-responsive sensory neurons; biological activity is abolished by antibodies raised against NGF. These results suggest that molecules with BDNF-like activity may be produced by cells in the peripheral nervous system and that the BDNF-like activity in fibroblasts and Schwann cells is derived from molecules immunologically related to NGF. In support of this concept, antibodies against NGF have been found to reduce the biological activity of recombinant BDNF in culture and to cross-react with BDNF on Western blots.</description><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>Antibodies - pharmacology</subject><subject>Axons - drug effects</subject><subject>Axons - physiology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Brain - metabolism</subject><subject>Brain-Derived Neurotrophic Factor</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Chick Embryo</subject><subject>Culture Media - pharmacology</subject><subject>Fibroblasts - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Metabolisms and neurohumoral controls</subject><subject>Mice</subject><subject>Nerve Growth Factors - immunology</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - immunology</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neurons, Afferent - drug effects</subject><subject>Nitrogen metabolism. Proteins. Glycoproteins. Nucleic acids. 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Psychology</topic><topic>Metabolisms and neurohumoral controls</topic><topic>Mice</topic><topic>Nerve Growth Factors - immunology</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - immunology</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neurons, Afferent - drug effects</topic><topic>Nitrogen metabolism. Proteins. Glycoproteins. Nucleic acids. Collagen</topic><topic>Rats</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Schwann Cells - metabolism</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Acheson, Ann</creatorcontrib><creatorcontrib>Barker, Philip A.</creatorcontrib><creatorcontrib>Alderson, Ralph F.</creatorcontrib><creatorcontrib>Miller, Freda D.</creatorcontrib><creatorcontrib>Murphy, Richard A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuron (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Acheson, Ann</au><au>Barker, Philip A.</au><au>Alderson, Ralph F.</au><au>Miller, Freda D.</au><au>Murphy, Richard A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of brain-derived neurotrophic factor-like activity in fibroblasts and Schwann cells: Inhibition by antibodies to NGF</atitle><jtitle>Neuron (Cambridge, Mass.)</jtitle><addtitle>Neuron</addtitle><date>1991-08-01</date><risdate>1991</risdate><volume>7</volume><issue>2</issue><spage>265</spage><epage>275</epage><pages>265-275</pages><issn>0896-6273</issn><eissn>1097-4199</eissn><coden>NERNET</coden><abstract>mRNA coding for brain-derived neurotrophic factor (BDNF) has been detected in cultured L929 fibroblasts, rat dermal fibroblasts, and sciatic nerve Schwann cells, as well as in rat skin. Medium conditioned by cultured fibroblasts and Schwann cells also stimulates neurite growth from retinal explants and promotes the survival in culture of BDNF-responsive sensory neurons; biological activity is abolished by antibodies raised against NGF. These results suggest that molecules with BDNF-like activity may be produced by cells in the peripheral nervous system and that the BDNF-like activity in fibroblasts and Schwann cells is derived from molecules immunologically related to NGF. In support of this concept, antibodies against NGF have been found to reduce the biological activity of recombinant BDNF in culture and to cross-react with BDNF on Western blots.</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>1873030</pmid><doi>10.1016/0896-6273(91)90265-2</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Antibodies - immunology Antibodies - pharmacology Axons - drug effects Axons - physiology Biological and medical sciences Blotting, Western Brain - metabolism Brain-Derived Neurotrophic Factor Cell Survival - drug effects Cells, Cultured Chick Embryo Culture Media - pharmacology Fibroblasts - metabolism Fundamental and applied biological sciences. Psychology Metabolisms and neurohumoral controls Mice Nerve Growth Factors - immunology Nerve Tissue Proteins - genetics Nerve Tissue Proteins - immunology Nerve Tissue Proteins - metabolism Neurons, Afferent - drug effects Nitrogen metabolism. Proteins. Glycoproteins. Nucleic acids. Collagen Rats RNA, Messenger - genetics RNA, Messenger - metabolism Schwann Cells - metabolism Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Detection of brain-derived neurotrophic factor-like activity in fibroblasts and Schwann cells: Inhibition by antibodies to NGF |
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