Fertilization in vitro increases non-disjunction during early cleavage divisions in a mouse model system
BACKGROUND: We have been studying an unusual mouse—the BALB/cWt (Wt) male—in which the Y chromosome is susceptible to high rates of mitotic non-disjunction, particularly at the first two cleavage divisions. As these are the same divisions that human embryos generated through assisted reproductive te...
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Veröffentlicht in: | Human reproduction (Oxford) 2002-09, Vol.17 (9), p.2362-2367 |
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description | BACKGROUND: We have been studying an unusual mouse—the BALB/cWt (Wt) male—in which the Y chromosome is susceptible to high rates of mitotic non-disjunction, particularly at the first two cleavage divisions. As these are the same divisions that human embryos generated through assisted reproductive technology must complete in an artificial setting, analysis of the Wt Y chromosome allows us to examine the effect of fertilization and culture in vitro on mammalian chromosome segregation. METHODS: We performed standard mouse IVF, cultured embryos in 5% CO2 in air or in a lowered oxygen atmosphere, and used fluorescence in-situ hybridization to examine the sex chromosome constitutions of 2-, 4-, 8- and 16-cell stage Wt Y-bearing embryos. RESULTS: We observed a significant increase in mosaic sex chromosome aneuploidy at each embryonic stage in embryos cultured in 5% CO2 in air, but under lowered oxygen conditions mosaicism returned to control (in-vivo) levels. CONCLUSIONS: Our results demonstrate that slight alterations in in-vitro conditions may have a considerable impact on the genetic quality of assisted reproductive technology-derived embryos and suggest that the genetic quality of embryos should be a fundamental concern in the development of new culture systems for clinical use. |
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As these are the same divisions that human embryos generated through assisted reproductive technology must complete in an artificial setting, analysis of the Wt Y chromosome allows us to examine the effect of fertilization and culture in vitro on mammalian chromosome segregation. METHODS: We performed standard mouse IVF, cultured embryos in 5% CO2 in air or in a lowered oxygen atmosphere, and used fluorescence in-situ hybridization to examine the sex chromosome constitutions of 2-, 4-, 8- and 16-cell stage Wt Y-bearing embryos. RESULTS: We observed a significant increase in mosaic sex chromosome aneuploidy at each embryonic stage in embryos cultured in 5% CO2 in air, but under lowered oxygen conditions mosaicism returned to control (in-vivo) levels. CONCLUSIONS: Our results demonstrate that slight alterations in in-vitro conditions may have a considerable impact on the genetic quality of assisted reproductive technology-derived embryos and suggest that the genetic quality of embryos should be a fundamental concern in the development of new culture systems for clinical use.</description><identifier>ISSN: 0268-1161</identifier><identifier>ISSN: 1460-2350</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/humrep/17.9.2362</identifier><identifier>PMID: 12202426</identifier><identifier>CODEN: HUREEE</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Aneuploidy ; Animals ; Biological and medical sciences ; Birth control ; Chromosome Segregation - physiology ; Cleavage Stage, Ovum - physiology ; Embryo, Mammalian - physiology ; Embryonic and Fetal Development ; Fertilization in Vitro ; Gynecology. Andrology. Obstetrics ; Male ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Mosaicism ; non-disjunction ; Nondisjunction, Genetic ; Oxygen - metabolism ; preimplantation embryo ; Sterility. Assisted procreation ; Y chromosome ; Y Chromosome - genetics</subject><ispartof>Human reproduction (Oxford), 2002-09, Vol.17 (9), p.2362-2367</ispartof><rights>European Society of Human Reproduction and Embryology 2002</rights><rights>2002 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Sep 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c492t-348048fc7ceac559f868c596ce03c156e5ff5bfe89bb9a022d3c881957f016b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13877766$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12202426$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bean, Christopher J.</creatorcontrib><creatorcontrib>Hassold, Terry J.</creatorcontrib><creatorcontrib>Judis, LuAnn</creatorcontrib><creatorcontrib>Hunt, Patricia A.</creatorcontrib><title>Fertilization in vitro increases non-disjunction during early cleavage divisions in a mouse model system</title><title>Human reproduction (Oxford)</title><addtitle>Hum. Reprod</addtitle><addtitle>Hum. Reprod</addtitle><description>BACKGROUND: We have been studying an unusual mouse—the BALB/cWt (Wt) male—in which the Y chromosome is susceptible to high rates of mitotic non-disjunction, particularly at the first two cleavage divisions. As these are the same divisions that human embryos generated through assisted reproductive technology must complete in an artificial setting, analysis of the Wt Y chromosome allows us to examine the effect of fertilization and culture in vitro on mammalian chromosome segregation. METHODS: We performed standard mouse IVF, cultured embryos in 5% CO2 in air or in a lowered oxygen atmosphere, and used fluorescence in-situ hybridization to examine the sex chromosome constitutions of 2-, 4-, 8- and 16-cell stage Wt Y-bearing embryos. RESULTS: We observed a significant increase in mosaic sex chromosome aneuploidy at each embryonic stage in embryos cultured in 5% CO2 in air, but under lowered oxygen conditions mosaicism returned to control (in-vivo) levels. CONCLUSIONS: Our results demonstrate that slight alterations in in-vitro conditions may have a considerable impact on the genetic quality of assisted reproductive technology-derived embryos and suggest that the genetic quality of embryos should be a fundamental concern in the development of new culture systems for clinical use.</description><subject>Aneuploidy</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Chromosome Segregation - physiology</subject><subject>Cleavage Stage, Ovum - physiology</subject><subject>Embryo, Mammalian - physiology</subject><subject>Embryonic and Fetal Development</subject><subject>Fertilization in Vitro</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mosaicism</subject><subject>non-disjunction</subject><subject>Nondisjunction, Genetic</subject><subject>Oxygen - metabolism</subject><subject>preimplantation embryo</subject><subject>Sterility. Assisted procreation</subject><subject>Y chromosome</subject><subject>Y Chromosome - genetics</subject><issn>0268-1161</issn><issn>1460-2350</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0c1rFDEUAPAgFrtW755kEPQis83H5OsoxdrCoh4WkV5CJvPSZp2PbTKzuP71ZjtLC568JIH83svLewi9IXhJsGbnd1MXYXtO5FIvKRP0GVqQSuCSMo6fowWmQpWECHKKXqa0wTgflXiBTgmlmFZULNDdJcQxtOGPHcPQF6EvdmGMQz64CDZBKvqhL5uQNlPvHkgzxdDfFmBjuy9cC3Znb6Fowi6kfJ0OKWzRDVOCvDbQFmmfRuheoRNv2wSvj_sZWl9-Xl9clatvX64vPq1KV2k6lqxSuFLeSQfWca69EspxLRxg5ggXwL3ntQel61pbTGnDnFJEc-nz72p2hj7MabdxuJ8gjaYLyUHb2h5yTUZSXFFZyQzf_QM3wxT7XJqhhGiiFOUZ4Rm5OKQUwZttDJ2Ne0OwOUzAzBMwRBptDhPIIW-Peae6g-Yp4NjyDN4fgU3Otj7a3oX05JiSUoqD-zi7Ydr-z7PlrENu9u9Hb-MvIyST3Fz9vDFfb1Z8vf7-w1TsL9KIrwY</recordid><startdate>20020901</startdate><enddate>20020901</enddate><creator>Bean, Christopher J.</creator><creator>Hassold, Terry J.</creator><creator>Judis, LuAnn</creator><creator>Hunt, Patricia A.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20020901</creationdate><title>Fertilization in vitro increases non-disjunction during early cleavage divisions in a mouse model system</title><author>Bean, Christopher J. ; Hassold, Terry J. ; Judis, LuAnn ; Hunt, Patricia A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-348048fc7ceac559f868c596ce03c156e5ff5bfe89bb9a022d3c881957f016b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Aneuploidy</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>Chromosome Segregation - physiology</topic><topic>Cleavage Stage, Ovum - physiology</topic><topic>Embryo, Mammalian - physiology</topic><topic>Embryonic and Fetal Development</topic><topic>Fertilization in Vitro</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mosaicism</topic><topic>non-disjunction</topic><topic>Nondisjunction, Genetic</topic><topic>Oxygen - metabolism</topic><topic>preimplantation embryo</topic><topic>Sterility. Assisted procreation</topic><topic>Y chromosome</topic><topic>Y Chromosome - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bean, Christopher J.</creatorcontrib><creatorcontrib>Hassold, Terry J.</creatorcontrib><creatorcontrib>Judis, LuAnn</creatorcontrib><creatorcontrib>Hunt, Patricia A.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bean, Christopher J.</au><au>Hassold, Terry J.</au><au>Judis, LuAnn</au><au>Hunt, Patricia A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fertilization in vitro increases non-disjunction during early cleavage divisions in a mouse model system</atitle><jtitle>Human reproduction (Oxford)</jtitle><stitle>Hum. Reprod</stitle><addtitle>Hum. Reprod</addtitle><date>2002-09-01</date><risdate>2002</risdate><volume>17</volume><issue>9</issue><spage>2362</spage><epage>2367</epage><pages>2362-2367</pages><issn>0268-1161</issn><issn>1460-2350</issn><eissn>1460-2350</eissn><coden>HUREEE</coden><abstract>BACKGROUND: We have been studying an unusual mouse—the BALB/cWt (Wt) male—in which the Y chromosome is susceptible to high rates of mitotic non-disjunction, particularly at the first two cleavage divisions. As these are the same divisions that human embryos generated through assisted reproductive technology must complete in an artificial setting, analysis of the Wt Y chromosome allows us to examine the effect of fertilization and culture in vitro on mammalian chromosome segregation. METHODS: We performed standard mouse IVF, cultured embryos in 5% CO2 in air or in a lowered oxygen atmosphere, and used fluorescence in-situ hybridization to examine the sex chromosome constitutions of 2-, 4-, 8- and 16-cell stage Wt Y-bearing embryos. RESULTS: We observed a significant increase in mosaic sex chromosome aneuploidy at each embryonic stage in embryos cultured in 5% CO2 in air, but under lowered oxygen conditions mosaicism returned to control (in-vivo) levels. CONCLUSIONS: Our results demonstrate that slight alterations in in-vitro conditions may have a considerable impact on the genetic quality of assisted reproductive technology-derived embryos and suggest that the genetic quality of embryos should be a fundamental concern in the development of new culture systems for clinical use.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>12202426</pmid><doi>10.1093/humrep/17.9.2362</doi><tpages>6</tpages></addata></record> |
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subjects | Aneuploidy Animals Biological and medical sciences Birth control Chromosome Segregation - physiology Cleavage Stage, Ovum - physiology Embryo, Mammalian - physiology Embryonic and Fetal Development Fertilization in Vitro Gynecology. Andrology. Obstetrics Male Medical sciences Mice Mice, Inbred BALB C Mosaicism non-disjunction Nondisjunction, Genetic Oxygen - metabolism preimplantation embryo Sterility. Assisted procreation Y chromosome Y Chromosome - genetics |
title | Fertilization in vitro increases non-disjunction during early cleavage divisions in a mouse model system |
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