Two bacteriophage T4 base plate genes (25 and 26) and the DNA repair Gene uvsY belong to spatially and temporally overlapping transcription units
The bacteriophage T4 DNA recombination-repair gene uvsY located at or near an origin of DNA replication and adjacent to the late base plate genes 25 and 26. Our present results reveal a complex transcription pattern in the region encompassing these genes. Most significantly, uvsY and two ORFs, downs...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1991-09, Vol.184 (1), p.359-369 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Gruidl, Michael E. Chen, Tony C. Gargano, Silvana Storlazzi, Aurora Cascino, Antonio Mosig, Gisela |
| description | The bacteriophage T4 DNA recombination-repair gene
uvsY located at or near an origin of DNA replication and adjacent to the late base plate genes 25 and 26. Our present results reveal a complex transcription pattern in the region encompassing these genes. Most significantly,
uvsY and two ORFs, downstream of it, all of which are transcribed from a middle promoter before the onset of DNA replication, are also part of a larger late transcription unit which includes the base plate genes 25 and 26. The late genes 25 and 26 are transcribed not only late, but also early from one or several early promoters further upstream. Translation, however, is inhibited by secondary structures which sequester the ribosome binding site in the early transcript. We discuss possible advantages of these transcriptional patterns for T4 DNA recombination, replication, and repair. The predicted and
in vivo-expressed 23.9-kDa product of gene 26 is smaller than the reported size of gene 26 protein isolated from base plates, suggesting that nascent gp26 might be processed to a larger protein during assembly. |
| doi_str_mv | 10.1016/0042-6822(91)90852-3 |
| format | Article |
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uvsY located at or near an origin of DNA replication and adjacent to the late base plate genes 25 and 26. Our present results reveal a complex transcription pattern in the region encompassing these genes. Most significantly,
uvsY and two ORFs, downstream of it, all of which are transcribed from a middle promoter before the onset of DNA replication, are also part of a larger late transcription unit which includes the base plate genes 25 and 26. The late genes 25 and 26 are transcribed not only late, but also early from one or several early promoters further upstream. Translation, however, is inhibited by secondary structures which sequester the ribosome binding site in the early transcript. We discuss possible advantages of these transcriptional patterns for T4 DNA recombination, replication, and repair. The predicted and
in vivo-expressed 23.9-kDa product of gene 26 is smaller than the reported size of gene 26 protein isolated from base plates, suggesting that nascent gp26 might be processed to a larger protein during assembly.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(91)90852-3</identifier><identifier>PMID: 1871975</identifier><identifier>CODEN: VIRLAX</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Base Sequence ; Biological and medical sciences ; Blotting, Northern ; Cloning, Molecular ; DNA Repair ; DNA, Viral - genetics ; DNA, Viral - isolation & purification ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Viral ; Genetic Complementation Test ; Genetics ; Microbiology ; Models, Structural ; Molecular Sequence Data ; Nucleic Acid Conformation ; Promoter Regions, Genetic ; Restriction Mapping ; RNA Probes ; RNA, Viral - genetics ; RNA, Viral - isolation & purification ; T-Phages - genetics ; Transcription, Genetic ; Virology</subject><ispartof>Virology (New York, N.Y.), 1991-09, Vol.184 (1), p.359-369</ispartof><rights>1991 Academic Press, Inc.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0042-6822(91)90852-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3538,27906,27907,45977</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5094645$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1871975$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gruidl, Michael E.</creatorcontrib><creatorcontrib>Chen, Tony C.</creatorcontrib><creatorcontrib>Gargano, Silvana</creatorcontrib><creatorcontrib>Storlazzi, Aurora</creatorcontrib><creatorcontrib>Cascino, Antonio</creatorcontrib><creatorcontrib>Mosig, Gisela</creatorcontrib><title>Two bacteriophage T4 base plate genes (25 and 26) and the DNA repair Gene uvsY belong to spatially and temporally overlapping transcription units</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>The bacteriophage T4 DNA recombination-repair gene
uvsY located at or near an origin of DNA replication and adjacent to the late base plate genes 25 and 26. Our present results reveal a complex transcription pattern in the region encompassing these genes. Most significantly,
uvsY and two ORFs, downstream of it, all of which are transcribed from a middle promoter before the onset of DNA replication, are also part of a larger late transcription unit which includes the base plate genes 25 and 26. The late genes 25 and 26 are transcribed not only late, but also early from one or several early promoters further upstream. Translation, however, is inhibited by secondary structures which sequester the ribosome binding site in the early transcript. We discuss possible advantages of these transcriptional patterns for T4 DNA recombination, replication, and repair. The predicted and
in vivo-expressed 23.9-kDa product of gene 26 is smaller than the reported size of gene 26 protein isolated from base plates, suggesting that nascent gp26 might be processed to a larger protein during assembly.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Cloning, Molecular</subject><subject>DNA Repair</subject><subject>DNA, Viral - genetics</subject><subject>DNA, Viral - isolation & purification</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Viral</subject><subject>Genetic Complementation Test</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Models, Structural</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Promoter Regions, Genetic</subject><subject>Restriction Mapping</subject><subject>RNA Probes</subject><subject>RNA, Viral - genetics</subject><subject>RNA, Viral - isolation & purification</subject><subject>T-Phages - genetics</subject><subject>Transcription, Genetic</subject><subject>Virology</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kc9u1DAQxi0EKtuWNwDJB4TaQ6j_JY4vSFULBamCy_bAyZokk62RNzZ2sqiPwRs32V319GlmfjPSNx8h7zn7zBmvrhhToqhqIS4MvzSsLkUhX5EVZ6YqmFT8NVm9IG_Jac5_2FxrzU7ICa81N7pckf_rf4E20I6YXIiPsEG6VnMjI40eRqQbHDDTC1FSGDoqqsu9jo9Ib39e04QRXKJ3M0SnXf5NG_Rh2NAx0BxhdOD902EBtzGkfRl2mDzE6BYuwZDb5OLowkCnwY35nLzpwWd8d9Qz8vDt6_rme3H_6-7HzfV9gVzqslAKe12WTa0bLqATlWDQ6FoJ2ctScGEMU1K1XVV3fV-ZjusepIa-VB0D1I08I58Od2MKfyfMo9263KL3MGCYstWCyVobNoMfjuDUbLGzMbktpCd7_OE8_3icQ27B97Ol1uUXrGRGVWrBvhwwnE3tHCabW4dDi51L2I62C85yZpdo7ZKbXXKzhtt9tFbKZ67ZlI4</recordid><startdate>199109</startdate><enddate>199109</enddate><creator>Gruidl, Michael E.</creator><creator>Chen, Tony C.</creator><creator>Gargano, Silvana</creator><creator>Storlazzi, Aurora</creator><creator>Cascino, Antonio</creator><creator>Mosig, Gisela</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199109</creationdate><title>Two bacteriophage T4 base plate genes (25 and 26) and the DNA repair Gene uvsY belong to spatially and temporally overlapping transcription units</title><author>Gruidl, Michael E. ; Chen, Tony C. ; Gargano, Silvana ; Storlazzi, Aurora ; Cascino, Antonio ; Mosig, Gisela</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e1375-44ef755b87b12ad2620ab78423f35212990434cd68dff69d17fa37af54d0ae7b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Cloning, Molecular</topic><topic>DNA Repair</topic><topic>DNA, Viral - genetics</topic><topic>DNA, Viral - isolation & purification</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Viral</topic><topic>Genetic Complementation Test</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Models, Structural</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Promoter Regions, Genetic</topic><topic>Restriction Mapping</topic><topic>RNA Probes</topic><topic>RNA, Viral - genetics</topic><topic>RNA, Viral - isolation & purification</topic><topic>T-Phages - genetics</topic><topic>Transcription, Genetic</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gruidl, Michael E.</creatorcontrib><creatorcontrib>Chen, Tony C.</creatorcontrib><creatorcontrib>Gargano, Silvana</creatorcontrib><creatorcontrib>Storlazzi, Aurora</creatorcontrib><creatorcontrib>Cascino, Antonio</creatorcontrib><creatorcontrib>Mosig, Gisela</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gruidl, Michael E.</au><au>Chen, Tony C.</au><au>Gargano, Silvana</au><au>Storlazzi, Aurora</au><au>Cascino, Antonio</au><au>Mosig, Gisela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two bacteriophage T4 base plate genes (25 and 26) and the DNA repair Gene uvsY belong to spatially and temporally overlapping transcription units</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1991-09</date><risdate>1991</risdate><volume>184</volume><issue>1</issue><spage>359</spage><epage>369</epage><pages>359-369</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>The bacteriophage T4 DNA recombination-repair gene
uvsY located at or near an origin of DNA replication and adjacent to the late base plate genes 25 and 26. Our present results reveal a complex transcription pattern in the region encompassing these genes. Most significantly,
uvsY and two ORFs, downstream of it, all of which are transcribed from a middle promoter before the onset of DNA replication, are also part of a larger late transcription unit which includes the base plate genes 25 and 26. The late genes 25 and 26 are transcribed not only late, but also early from one or several early promoters further upstream. Translation, however, is inhibited by secondary structures which sequester the ribosome binding site in the early transcript. We discuss possible advantages of these transcriptional patterns for T4 DNA recombination, replication, and repair. The predicted and
in vivo-expressed 23.9-kDa product of gene 26 is smaller than the reported size of gene 26 protein isolated from base plates, suggesting that nascent gp26 might be processed to a larger protein during assembly.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1871975</pmid><doi>10.1016/0042-6822(91)90852-3</doi><tpages>11</tpages></addata></record> |
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| ispartof | Virology (New York, N.Y.), 1991-09, Vol.184 (1), p.359-369 |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Base Sequence Biological and medical sciences Blotting, Northern Cloning, Molecular DNA Repair DNA, Viral - genetics DNA, Viral - isolation & purification Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Viral Genetic Complementation Test Genetics Microbiology Models, Structural Molecular Sequence Data Nucleic Acid Conformation Promoter Regions, Genetic Restriction Mapping RNA Probes RNA, Viral - genetics RNA, Viral - isolation & purification T-Phages - genetics Transcription, Genetic Virology |
| title | Two bacteriophage T4 base plate genes (25 and 26) and the DNA repair Gene uvsY belong to spatially and temporally overlapping transcription units |
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