Replacement of vertebrate serum with lipids and other factors in the culture of invertebrate cells, tissues, parasites, and pathogens

Culture medium supplementation with vertebrate serum results in the selection of fibroblastoid insect cell lines and a general decline during continuous subculturing of both morphologic and functional differentiation of the surviving cells. Essential lipid mixtures can substitute for vertebrate seru...

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Veröffentlicht in:	In Vitro Cellular & Developmental Biology - Animal 1991-06, Vol.27A (6), p.470-478
1. Verfasser:	Goodwin, R.H. (Montana State University, Bozeman, MT)
Format:	Artikel
Sprache:	eng
Schlagworte:	ACIDE GRAS ACIDOS GRASOS Animal cells Animals Biological and medical sciences CELL CULTURE Cell culture techniques Cell cultures. Hybridization. Fusion CELL LINES CELL MEMBRANES Cells Cells, Cultured cholesterol CITOPLASMA CULTIVO DE CELULAS CULTURE DE CELLULE CULTURE MEDIA Culture Media - analysis Culture Media - pharmacology Cultured cells CYTOPLASM CYTOPLASME FATTY ACIDS Fatty Acids, Unsaturated - analysis Fatty Acids, Unsaturated - pharmacology Female FIBROBLASTE fibroblastoid cell lines FIBROBLASTOS FIBROBLASTS FOSFOLIPIDOS Fundamental and applied biological sciences. Psychology growth factors Humans INSECTA Invertebrates Invertebrates - cytology Invertebrates - drug effects Invertebrates - parasitology LIPID METABOLISM LIPIDE LIPIDOS LIPIDS Lipids - analysis Lipids - pharmacology LIPOSOMES LITERATURE REVIEWS MEDIO DE CULTIVO MEMBRANAS CELULARES MEMBRANE CELLULAIRE METABOLISME DES LIPIDES METABOLISMO DE LIPIDOS Middle Aged MILIEU DE CULTURE Molecular and cellular biology Parasites - cytology Parasites - drug effects PHOSPHATIDE PHOSPHOLIPIDS proteoliposomes reviews serum sterols Sterols - analysis Sterols - pharmacology substitution TCA Session: In-Depth Invertebrate Cell Culture tissue culture Vertebrates
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