Determination of Alpha Hydroxy Acids in Fruits by Capillary Electrophoresis
Alpha hydroxy acids, malic acid, citric acid, tartaric acid, glycolic acid and lactic acid, were analyzed simultaneously using capillary electrophoresis with direct UV detection at 200 nm. The separation was carried out with uncoated fused-silica (50 cm × 50 μm i.d.), pressure injection at 15 psi s...
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| Veröffentlicht in: | Analytical Sciences 2002, Vol.18(8), pp.893-896 |
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| creator | VORARAT, Suwanna AROMDEE, Chantana PODOKMAI, Yodporn |
| description | Alpha hydroxy acids, malic acid, citric acid, tartaric acid, glycolic acid and lactic acid, were analyzed simultaneously using capillary electrophoresis with direct UV detection at 200 nm. The separation was carried out with uncoated fused-silica (50 cm × 50 μm i.d.), pressure injection at 15 psi s and operated at -15 kV potential. The separation buffers were prepared with 180 mM Na2HPO4, 1 mM cetyltrimethylammonium bromide and 15% (v/v) methanol and adjusted to pH 7.2 by phosphoric acid. Validation was performed for citric acid and malic acid. The obtained parameters were adequate and the limits of detection were 2.5 and 5 μg ml-1 for citric acid and malic acid, respectively. AHAs from natural fruit juices (orange and grape) were determined and measured with this method. |
| doi_str_mv | 10.2116/analsci.18.893 |
| format | Article |
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The separation was carried out with uncoated fused-silica (50 cm × 50 μm i.d.), pressure injection at 15 psi s and operated at -15 kV potential. The separation buffers were prepared with 180 mM Na2HPO4, 1 mM cetyltrimethylammonium bromide and 15% (v/v) methanol and adjusted to pH 7.2 by phosphoric acid. Validation was performed for citric acid and malic acid. The obtained parameters were adequate and the limits of detection were 2.5 and 5 μg ml-1 for citric acid and malic acid, respectively. AHAs from natural fruit juices (orange and grape) were determined and measured with this method.</description><identifier>ISSN: 0910-6340</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.18.893</identifier><identifier>PMID: 12200835</identifier><identifier>CODEN: ANSCEN</identifier><language>eng</language><publisher>Tokyo: The Japan Society for Analytical Chemistry</publisher><subject>Analytical chemistry ; Biological and medical sciences ; Buffers ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Citric Acid - analysis ; Citrus - chemistry ; Electrophoresis, Capillary - methods ; Exact sciences and technology ; Food industries ; Fruit - chemistry ; Fundamental and applied biological sciences. Psychology ; Hydroxy Acids - analysis ; Malates - analysis ; Non alcoholic beverage industries and mineral waters ; Other chromatographic methods ; Sensitivity and Specificity ; Vitis - chemistry</subject><ispartof>Analytical Sciences, 2002, Vol.18(8), pp.893-896</ispartof><rights>2002 by The Japan Society for Analytical Chemistry</rights><rights>2002 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c601t-c3f9776e69d91c2829d214f1a1760f9fca60b5fe432ffb37f031783525e13d8f3</citedby><cites>FETCH-LOGICAL-c601t-c3f9776e69d91c2829d214f1a1760f9fca60b5fe432ffb37f031783525e13d8f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,1884,4025,27928,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13936599$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12200835$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VORARAT, Suwanna</creatorcontrib><creatorcontrib>AROMDEE, Chantana</creatorcontrib><creatorcontrib>PODOKMAI, Yodporn</creatorcontrib><title>Determination of Alpha Hydroxy Acids in Fruits by Capillary Electrophoresis</title><title>Analytical Sciences</title><addtitle>Anal Sci</addtitle><description>Alpha hydroxy acids, malic acid, citric acid, tartaric acid, glycolic acid and lactic acid, were analyzed simultaneously using capillary electrophoresis with direct UV detection at 200 nm. The separation was carried out with uncoated fused-silica (50 cm × 50 μm i.d.), pressure injection at 15 psi s and operated at -15 kV potential. The separation buffers were prepared with 180 mM Na2HPO4, 1 mM cetyltrimethylammonium bromide and 15% (v/v) methanol and adjusted to pH 7.2 by phosphoric acid. Validation was performed for citric acid and malic acid. The obtained parameters were adequate and the limits of detection were 2.5 and 5 μg ml-1 for citric acid and malic acid, respectively. AHAs from natural fruit juices (orange and grape) were determined and measured with this method.</description><subject>Analytical chemistry</subject><subject>Biological and medical sciences</subject><subject>Buffers</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Citric Acid - analysis</subject><subject>Citrus - chemistry</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Exact sciences and technology</subject><subject>Food industries</subject><subject>Fruit - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydroxy Acids - analysis</subject><subject>Malates - analysis</subject><subject>Non alcoholic beverage industries and mineral waters</subject><subject>Other chromatographic methods</subject><subject>Sensitivity and Specificity</subject><subject>Vitis - chemistry</subject><issn>0910-6340</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkEFv2yAYhlG1qs26XXeckKbt5owPMDbHKEuWqpV2ac-IYFiIHOOBLS3_vkSxGrUXOPDwve_3IPQFyJwCiJ-6020yfg71vJbsCs2A8bqglIsPaEYkkEIwTm7Rx5T2hACtKb1Bt0ApITUrZ-jhlx1sPPhODz50ODi8aPudxptjE8P_I14Y3yTsO7yOox8S3h7xUve-bXU84lVrzRBDvwvRJp8-oWuX29jP032Hnterp-WmePzz-365eCyMIDAUhjlZVcIK2UgwuZFsKHAHGipBnHRGC7ItneWMOrdllSMMqlyWlhZYUzt2h36c5_Yx_BttGtTBJ2Nzp86GMamKkrwzrTL47R24D2M8GVPAeV1yTiqZqfmZMjGkFK1TffSHvJ8Cok6S1SRZQa2y5Pzh6zR23B5sc8Enqxn4PgE6Gd26qDvj04VjkolSnpKXZ26fBv3XvgI6Dt609k3u-cjxr69mp6OyHXsBylydog</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>VORARAT, Suwanna</creator><creator>AROMDEE, Chantana</creator><creator>PODOKMAI, Yodporn</creator><general>The Japan Society for Analytical Chemistry</general><general>Japan Society for Analytical Chemistry</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2002</creationdate><title>Determination of Alpha Hydroxy Acids in Fruits by Capillary Electrophoresis</title><author>VORARAT, Suwanna ; AROMDEE, Chantana ; PODOKMAI, Yodporn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c601t-c3f9776e69d91c2829d214f1a1760f9fca60b5fe432ffb37f031783525e13d8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Analytical chemistry</topic><topic>Biological and medical sciences</topic><topic>Buffers</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Citric Acid - analysis</topic><topic>Citrus - chemistry</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Exact sciences and technology</topic><topic>Food industries</topic><topic>Fruit - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydroxy Acids - analysis</topic><topic>Malates - analysis</topic><topic>Non alcoholic beverage industries and mineral waters</topic><topic>Other chromatographic methods</topic><topic>Sensitivity and Specificity</topic><topic>Vitis - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VORARAT, Suwanna</creatorcontrib><creatorcontrib>AROMDEE, Chantana</creatorcontrib><creatorcontrib>PODOKMAI, Yodporn</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>VORARAT, Suwanna</au><au>AROMDEE, Chantana</au><au>PODOKMAI, Yodporn</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Alpha Hydroxy Acids in Fruits by Capillary Electrophoresis</atitle><jtitle>Analytical Sciences</jtitle><addtitle>Anal Sci</addtitle><date>2002</date><risdate>2002</risdate><volume>18</volume><issue>8</issue><spage>893</spage><epage>896</epage><pages>893-896</pages><issn>0910-6340</issn><eissn>1348-2246</eissn><coden>ANSCEN</coden><abstract>Alpha hydroxy acids, malic acid, citric acid, tartaric acid, glycolic acid and lactic acid, were analyzed simultaneously using capillary electrophoresis with direct UV detection at 200 nm. The separation was carried out with uncoated fused-silica (50 cm × 50 μm i.d.), pressure injection at 15 psi s and operated at -15 kV potential. The separation buffers were prepared with 180 mM Na2HPO4, 1 mM cetyltrimethylammonium bromide and 15% (v/v) methanol and adjusted to pH 7.2 by phosphoric acid. Validation was performed for citric acid and malic acid. The obtained parameters were adequate and the limits of detection were 2.5 and 5 μg ml-1 for citric acid and malic acid, respectively. AHAs from natural fruit juices (orange and grape) were determined and measured with this method.</abstract><cop>Tokyo</cop><pub>The Japan Society for Analytical Chemistry</pub><pmid>12200835</pmid><doi>10.2116/analsci.18.893</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; SpringerNature Journals; Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Analytical chemistry Biological and medical sciences Buffers Chemistry Chromatographic methods and physical methods associated with chromatography Citric Acid - analysis Citrus - chemistry Electrophoresis, Capillary - methods Exact sciences and technology Food industries Fruit - chemistry Fundamental and applied biological sciences. Psychology Hydroxy Acids - analysis Malates - analysis Non alcoholic beverage industries and mineral waters Other chromatographic methods Sensitivity and Specificity Vitis - chemistry |
| title | Determination of Alpha Hydroxy Acids in Fruits by Capillary Electrophoresis |
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