Development and Evaluation of an Enzyme-Linked Immunosorbent Assay with Recombinant SAG2 for Diagnosis of Toxoplasma gondii Infection in Cats
Cats are pivotal in the transmission of Toxoplasma gondii. To develop a sensitive and specific serodiagnostic method for feline toxoplasmosis, surface antigen 2 (SAG2) of T. gondii was expressed in Escherichia coli and its diagnostic potential evaluated in an enzyme-linked immunosorbent assay (ELISA...
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| Veröffentlicht in: | The Journal of parasitology 2002-08, Vol.88 (4), p.804-807 |
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| container_title | The Journal of parasitology |
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| creator | Huang, X Xuan, X Kimbita, E. N Battur, B Miyazawa, T Fukumoto, S Mishima, M Makala, L. H Suzuki, H Sugimoto, C Nagasawa, H Fujisaki, K Mikami, T Igarashi, I |
| description | Cats are pivotal in the transmission of Toxoplasma gondii. To develop a sensitive and specific serodiagnostic method for feline toxoplasmosis, surface antigen 2 (SAG2) of T. gondii was expressed in Escherichia coli and its diagnostic potential evaluated in an enzyme-linked immunosorbent assay (ELISA). The ELISA with recombinant SAG2 (rSAG2) was able to differentiate very clearly between sera from cats experimentally infected with T. gondii and sera from normal cats. Serum samples collected from domestic cats in Japan were investigated by the ELISA, and the results were compared with those of a commercially available latex agglutination test (LAT) kit. Of the 192 samples screened, 42 (21.9%) were positive by ELISA. Among the 42 ELISA-positive samples, 39 were positive by LAT. There was a significant correlation between ELISA and LAT titers. All the 150 ELISA-negative samples were negative by LAT. These results indicate that the ELISA with rSAG2 expressed in E. coli should be a useful method for detection of T. gondii infection in cats. |
| doi_str_mv | 10.1645/0022-3395(2002)088[0804:DAEOAE]2.0.CO;2 |
| format | Article |
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N ; Battur, B ; Miyazawa, T ; Fukumoto, S ; Mishima, M ; Makala, L. H ; Suzuki, H ; Sugimoto, C ; Nagasawa, H ; Fujisaki, K ; Mikami, T ; Igarashi, I</creator><creatorcontrib>Huang, X ; Xuan, X ; Kimbita, E. N ; Battur, B ; Miyazawa, T ; Fukumoto, S ; Mishima, M ; Makala, L. H ; Suzuki, H ; Sugimoto, C ; Nagasawa, H ; Fujisaki, K ; Mikami, T ; Igarashi, I</creatorcontrib><description>Cats are pivotal in the transmission of Toxoplasma gondii. To develop a sensitive and specific serodiagnostic method for feline toxoplasmosis, surface antigen 2 (SAG2) of T. gondii was expressed in Escherichia coli and its diagnostic potential evaluated in an enzyme-linked immunosorbent assay (ELISA). The ELISA with recombinant SAG2 (rSAG2) was able to differentiate very clearly between sera from cats experimentally infected with T. gondii and sera from normal cats. Serum samples collected from domestic cats in Japan were investigated by the ELISA, and the results were compared with those of a commercially available latex agglutination test (LAT) kit. Of the 192 samples screened, 42 (21.9%) were positive by ELISA. Among the 42 ELISA-positive samples, 39 were positive by LAT. There was a significant correlation between ELISA and LAT titers. All the 150 ELISA-negative samples were negative by LAT. These results indicate that the ELISA with rSAG2 expressed in E. coli should be a useful method for detection of T. gondii infection in cats.</description><identifier>ISSN: 0022-3395</identifier><identifier>EISSN: 1937-2345</identifier><identifier>DOI: 10.1645/0022-3395(2002)088[0804:DAEOAE]2.0.CO;2</identifier><identifier>PMID: 12197139</identifier><identifier>CODEN: JOPAA2</identifier><language>eng</language><publisher>Lawrence, KS: American Society of Parasitologists</publisher><subject>Animal digestion ; Animal protozoal diseases ; Animals ; Antibodies ; Antibodies, Protozoan - blood ; Antigens ; Antigens, Protozoan ; Antigens, Surface ; Biological and medical sciences ; Cat Diseases - diagnosis ; Cats ; Enzyme linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Escherichia coli - genetics ; Genetic Vectors ; Infections ; Infectious diseases ; Medical sciences ; Mucosa ; Nematode larvae ; Parasitic diseases ; Polymerase chain reaction ; Protozoal diseases ; Protozoan Proteins ; Recombinant Proteins ; RESEARCH NOTES ; Toxoplasma - immunology ; Toxoplasma - isolation & purification ; Toxoplasmosis ; Toxoplasmosis, Animal - blood ; Toxoplasmosis, Animal - diagnosis</subject><ispartof>The Journal of parasitology, 2002-08, Vol.88 (4), p.804-807</ispartof><rights>American Society of Parasitologists</rights><rights>Copyright 2002 American Society of Parasitologists</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b462t-66155c4e8c39e6263689504d897055301bfcf459d391438e3998ed3b7556b32f3</citedby><cites>FETCH-LOGICAL-b462t-66155c4e8c39e6263689504d897055301bfcf459d391438e3998ed3b7556b32f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1645/0022-3395(2002)088[0804:DAEOAE]2.0.CO;2$$EPDF$$P50$$Gbioone$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3285368$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>315,781,785,804,26983,27929,27930,52368,58022,58255</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13848483$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12197139$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, X</creatorcontrib><creatorcontrib>Xuan, X</creatorcontrib><creatorcontrib>Kimbita, E. N</creatorcontrib><creatorcontrib>Battur, B</creatorcontrib><creatorcontrib>Miyazawa, T</creatorcontrib><creatorcontrib>Fukumoto, S</creatorcontrib><creatorcontrib>Mishima, M</creatorcontrib><creatorcontrib>Makala, L. H</creatorcontrib><creatorcontrib>Suzuki, H</creatorcontrib><creatorcontrib>Sugimoto, C</creatorcontrib><creatorcontrib>Nagasawa, H</creatorcontrib><creatorcontrib>Fujisaki, K</creatorcontrib><creatorcontrib>Mikami, T</creatorcontrib><creatorcontrib>Igarashi, I</creatorcontrib><title>Development and Evaluation of an Enzyme-Linked Immunosorbent Assay with Recombinant SAG2 for Diagnosis of Toxoplasma gondii Infection in Cats</title><title>The Journal of parasitology</title><addtitle>J Parasitol</addtitle><description>Cats are pivotal in the transmission of Toxoplasma gondii. To develop a sensitive and specific serodiagnostic method for feline toxoplasmosis, surface antigen 2 (SAG2) of T. gondii was expressed in Escherichia coli and its diagnostic potential evaluated in an enzyme-linked immunosorbent assay (ELISA). The ELISA with recombinant SAG2 (rSAG2) was able to differentiate very clearly between sera from cats experimentally infected with T. gondii and sera from normal cats. Serum samples collected from domestic cats in Japan were investigated by the ELISA, and the results were compared with those of a commercially available latex agglutination test (LAT) kit. Of the 192 samples screened, 42 (21.9%) were positive by ELISA. Among the 42 ELISA-positive samples, 39 were positive by LAT. There was a significant correlation between ELISA and LAT titers. All the 150 ELISA-negative samples were negative by LAT. These results indicate that the ELISA with rSAG2 expressed in E. coli should be a useful method for detection of T. gondii infection in cats.</description><subject>Animal digestion</subject><subject>Animal protozoal diseases</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Protozoan - blood</subject><subject>Antigens</subject><subject>Antigens, Protozoan</subject><subject>Antigens, Surface</subject><subject>Biological and medical sciences</subject><subject>Cat Diseases - diagnosis</subject><subject>Cats</subject><subject>Enzyme linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Escherichia coli - genetics</subject><subject>Genetic Vectors</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Mucosa</subject><subject>Nematode larvae</subject><subject>Parasitic diseases</subject><subject>Polymerase chain reaction</subject><subject>Protozoal diseases</subject><subject>Protozoan Proteins</subject><subject>Recombinant Proteins</subject><subject>RESEARCH NOTES</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasma - isolation & purification</subject><subject>Toxoplasmosis</subject><subject>Toxoplasmosis, Animal - blood</subject><subject>Toxoplasmosis, Animal - diagnosis</subject><issn>0022-3395</issn><issn>1937-2345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqdkc1u1DAUhS0EokPhFZAXUMEiU__EiU1XUSZMRxppJCgrhCwncYpLYg9x0nZ4B94ZpxlR1siybB1999yrewA4x2iJk5idI0RIRKlg70j4vkecf0UcxR9WWbHLim9kiZb57oI8AQssaBoRGrOnYPG36gS88P4GIcTCfQ5OMMEixVQswO-VvtWt23faDlDZGha3qh3VYJyFrgkKLOyvQ6ejrbE_dA03XTda511fTgWZ9-oA78zwHX7SletKY1WQP2drAhvXw5VR14E2fvK6cvdu3yrfKXjtbG0M3NhGVw-tjIW5GvxL8KxRrdevju8p-PKxuMovo-1uvcmzbVTGCRmiJMGMVbHmFRU6IQlNuGAorrlIEWMU4bKpmpiJmgocU66pEFzXtEwZS0pKGnoKzmbffe9-jtoPsjO-0m2rrHajlylBFImUBXA9g1XvvO91I_e96VR_kBjJKRk57VhOO5ZTMjIkI6dk5JyMDIrMd5IEp9fHlmPZ6frR5xhFAN4eAeUr1Ta9spXxjxzlcTg0cG9m7sYPrv93HkJRKinhLOwjYMWMlcY5q_977j8akLmB</recordid><startdate>20020801</startdate><enddate>20020801</enddate><creator>Huang, X</creator><creator>Xuan, X</creator><creator>Kimbita, E. 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H</au><au>Suzuki, H</au><au>Sugimoto, C</au><au>Nagasawa, H</au><au>Fujisaki, K</au><au>Mikami, T</au><au>Igarashi, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and Evaluation of an Enzyme-Linked Immunosorbent Assay with Recombinant SAG2 for Diagnosis of Toxoplasma gondii Infection in Cats</atitle><jtitle>The Journal of parasitology</jtitle><addtitle>J Parasitol</addtitle><date>2002-08-01</date><risdate>2002</risdate><volume>88</volume><issue>4</issue><spage>804</spage><epage>807</epage><pages>804-807</pages><issn>0022-3395</issn><eissn>1937-2345</eissn><coden>JOPAA2</coden><abstract>Cats are pivotal in the transmission of Toxoplasma gondii. To develop a sensitive and specific serodiagnostic method for feline toxoplasmosis, surface antigen 2 (SAG2) of T. gondii was expressed in Escherichia coli and its diagnostic potential evaluated in an enzyme-linked immunosorbent assay (ELISA). The ELISA with recombinant SAG2 (rSAG2) was able to differentiate very clearly between sera from cats experimentally infected with T. gondii and sera from normal cats. Serum samples collected from domestic cats in Japan were investigated by the ELISA, and the results were compared with those of a commercially available latex agglutination test (LAT) kit. Of the 192 samples screened, 42 (21.9%) were positive by ELISA. Among the 42 ELISA-positive samples, 39 were positive by LAT. There was a significant correlation between ELISA and LAT titers. All the 150 ELISA-negative samples were negative by LAT. These results indicate that the ELISA with rSAG2 expressed in E. coli should be a useful method for detection of T. gondii infection in cats.</abstract><cop>Lawrence, KS</cop><pub>American Society of Parasitologists</pub><pmid>12197139</pmid><doi>10.1645/0022-3395(2002)088[0804:DAEOAE]2.0.CO;2</doi><tpages>4</tpages></addata></record> |
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| subjects | Animal digestion Animal protozoal diseases Animals Antibodies Antibodies, Protozoan - blood Antigens Antigens, Protozoan Antigens, Surface Biological and medical sciences Cat Diseases - diagnosis Cats Enzyme linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Escherichia coli - genetics Genetic Vectors Infections Infectious diseases Medical sciences Mucosa Nematode larvae Parasitic diseases Polymerase chain reaction Protozoal diseases Protozoan Proteins Recombinant Proteins RESEARCH NOTES Toxoplasma - immunology Toxoplasma - isolation & purification Toxoplasmosis Toxoplasmosis, Animal - blood Toxoplasmosis, Animal - diagnosis |
| title | Development and Evaluation of an Enzyme-Linked Immunosorbent Assay with Recombinant SAG2 for Diagnosis of Toxoplasma gondii Infection in Cats |
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