Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect
The molecular basis of most human male infertility arising from spermatogenesis disruption is poorly understood because of a lack of useful investigation systems. To study the roles of the supporting Sertoli cells in mammalian spermatogenesis, we improved an electroporation technique for seminiferou...
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| Veröffentlicht in: | Biology of reproduction 2002-09, Vol.67 (3), p.712-717 |
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| container_title | Biology of reproduction |
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| creator | YOMOGIDA, Kentaro YAGURA, Yo NISHIMUNE, Yoshitake |
| description | The molecular basis of most human male infertility arising from spermatogenesis disruption is poorly understood because of
a lack of useful investigation systems. To study the roles of the supporting Sertoli cells in mammalian spermatogenesis, we
improved an electroporation technique for seminiferous tubules in vivo. Because Sertoli cells barely proliferate in mature
testis, linear transgenes are not incorporated into the genome and quickly degrade. However, circular expression vector is
stably expressed in Sertoli cells for a long period. By electrotransformation of a complete cDNA, we rescued defective spermatogenesis
in infertile Sl 17H / Sl 17H mutant mice with partial dysfunction of stem cell factor in Sertoli cells. Application of this gene transfer system will
facilitate both the understanding of spermatogenesis and the development of new gene therapies for human male infertility. |
| doi_str_mv | 10.1095/biolreprod.101.001743 |
| format | Article |
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a lack of useful investigation systems. To study the roles of the supporting Sertoli cells in mammalian spermatogenesis, we
improved an electroporation technique for seminiferous tubules in vivo. Because Sertoli cells barely proliferate in mature
testis, linear transgenes are not incorporated into the genome and quickly degrade. However, circular expression vector is
stably expressed in Sertoli cells for a long period. By electrotransformation of a complete cDNA, we rescued defective spermatogenesis
in infertile Sl 17H / Sl 17H mutant mice with partial dysfunction of stem cell factor in Sertoli cells. Application of this gene transfer system will
facilitate both the understanding of spermatogenesis and the development of new gene therapies for human male infertility.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.101.001743</identifier><identifier>PMID: 12193376</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Actins - genetics ; Animals ; Biological and medical sciences ; Cell Differentiation ; DNA, Circular - genetics ; Electroporation ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genetic Therapy ; Green Fluorescent Proteins ; Infertility, Male - genetics ; Infertility, Male - therapy ; Luminescent Proteins - genetics ; Male ; Mammalian male genital system ; Mice ; Mice, Inbred C57BL ; Morphology. Physiology ; Promoter Regions, Genetic ; Seminiferous Tubules - cytology ; Sertoli Cells - metabolism ; Sperm Count ; Spermatogenesis - genetics ; Spermatozoa - cytology ; Stem Cell Factor - genetics ; Transfection - methods ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2002-09, Vol.67 (3), p.712-717</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13870831$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12193376$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YOMOGIDA, Kentaro</creatorcontrib><creatorcontrib>YAGURA, Yo</creatorcontrib><creatorcontrib>NISHIMUNE, Yoshitake</creatorcontrib><title>Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>The molecular basis of most human male infertility arising from spermatogenesis disruption is poorly understood because of
a lack of useful investigation systems. To study the roles of the supporting Sertoli cells in mammalian spermatogenesis, we
improved an electroporation technique for seminiferous tubules in vivo. Because Sertoli cells barely proliferate in mature
testis, linear transgenes are not incorporated into the genome and quickly degrade. However, circular expression vector is
stably expressed in Sertoli cells for a long period. By electrotransformation of a complete cDNA, we rescued defective spermatogenesis
in infertile Sl 17H / Sl 17H mutant mice with partial dysfunction of stem cell factor in Sertoli cells. Application of this gene transfer system will
facilitate both the understanding of spermatogenesis and the development of new gene therapies for human male infertility.</description><subject>Actins - genetics</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation</subject><subject>DNA, Circular - genetics</subject><subject>Electroporation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genetic Therapy</subject><subject>Green Fluorescent Proteins</subject><subject>Infertility, Male - genetics</subject><subject>Infertility, Male - therapy</subject><subject>Luminescent Proteins - genetics</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Morphology. Physiology</subject><subject>Promoter Regions, Genetic</subject><subject>Seminiferous Tubules - cytology</subject><subject>Sertoli Cells - metabolism</subject><subject>Sperm Count</subject><subject>Spermatogenesis - genetics</subject><subject>Spermatozoa - cytology</subject><subject>Stem Cell Factor - genetics</subject><subject>Transfection - methods</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMFO3DAQhq2KqizQRyjyBW6hY09iJ8dqoRQJVKnQc-Q4E9bISba2o4i3x1W34jSafz79Gn2MfRFwJaCpvnZu9oH2Ye7zLq4AhC7xA9uISjaFlqo-YhsAUAWiwmN2EuNLZkqU-IkdCykaRK02rL_xZFOY93MwiXr-FMwUn2mi4hdFu-TkcU9hNGn-G0YXuZv43TRQSM4Tf1iSmRJ_cJb46tKOG_6YT7N3fEve82sacv0Z-zgYH-nzYZ6y399vnrY_ivuft3fbb_fFTiqdCqFIl7Ybahx62wmE0vQNaVJVaZQtATurQVsJfS8qXTcKUFVQWW0bqKGr8ZRd_uvNWv4sFFM7umjzH2aieYmtloDQaJnB8wO4dCP17T640YTX9r-XDFwcABOt8UO2Yl1857DWUKN453buebe6QG0cjfe5Ftt1XZVusdVC4hu9H4Hc</recordid><startdate>20020901</startdate><enddate>20020901</enddate><creator>YOMOGIDA, Kentaro</creator><creator>YAGURA, Yo</creator><creator>NISHIMUNE, Yoshitake</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20020901</creationdate><title>Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect</title><author>YOMOGIDA, Kentaro ; YAGURA, Yo ; NISHIMUNE, Yoshitake</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h267t-16e74cbf83fdcb1304ad9e7e654a6c403bc707c20dd157896036505c7c9080b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Actins - genetics</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation</topic><topic>DNA, Circular - genetics</topic><topic>Electroporation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genetic Therapy</topic><topic>Green Fluorescent Proteins</topic><topic>Infertility, Male - genetics</topic><topic>Infertility, Male - therapy</topic><topic>Luminescent Proteins - genetics</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Morphology. Physiology</topic><topic>Promoter Regions, Genetic</topic><topic>Seminiferous Tubules - cytology</topic><topic>Sertoli Cells - metabolism</topic><topic>Sperm Count</topic><topic>Spermatogenesis - genetics</topic><topic>Spermatozoa - cytology</topic><topic>Stem Cell Factor - genetics</topic><topic>Transfection - methods</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YOMOGIDA, Kentaro</creatorcontrib><creatorcontrib>YAGURA, Yo</creatorcontrib><creatorcontrib>NISHIMUNE, Yoshitake</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YOMOGIDA, Kentaro</au><au>YAGURA, Yo</au><au>NISHIMUNE, Yoshitake</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2002-09-01</date><risdate>2002</risdate><volume>67</volume><issue>3</issue><spage>712</spage><epage>717</epage><pages>712-717</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>The molecular basis of most human male infertility arising from spermatogenesis disruption is poorly understood because of
a lack of useful investigation systems. To study the roles of the supporting Sertoli cells in mammalian spermatogenesis, we
improved an electroporation technique for seminiferous tubules in vivo. Because Sertoli cells barely proliferate in mature
testis, linear transgenes are not incorporated into the genome and quickly degrade. However, circular expression vector is
stably expressed in Sertoli cells for a long period. By electrotransformation of a complete cDNA, we rescued defective spermatogenesis
in infertile Sl 17H / Sl 17H mutant mice with partial dysfunction of stem cell factor in Sertoli cells. Application of this gene transfer system will
facilitate both the understanding of spermatogenesis and the development of new gene therapies for human male infertility.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>12193376</pmid><doi>10.1095/biolreprod.101.001743</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2002-09, Vol.67 (3), p.712-717 |
| issn | 0006-3363 1529-7268 |
| language | eng |
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| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Actins - genetics Animals Biological and medical sciences Cell Differentiation DNA, Circular - genetics Electroporation Fundamental and applied biological sciences. Psychology Gene Expression Genetic Therapy Green Fluorescent Proteins Infertility, Male - genetics Infertility, Male - therapy Luminescent Proteins - genetics Male Mammalian male genital system Mice Mice, Inbred C57BL Morphology. Physiology Promoter Regions, Genetic Seminiferous Tubules - cytology Sertoli Cells - metabolism Sperm Count Spermatogenesis - genetics Spermatozoa - cytology Stem Cell Factor - genetics Transfection - methods Vertebrates: reproduction |
| title | Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect |
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