Activation of a rice endogenous retrotransposon Tos17 in tissue culture is accompanied by cytosine demethylation and causes heritable alteration in methylation pattern of flanking genomic regions

Tos17 is a copia-like, cryptic retrotransposon of rice, but can be activated by tissue culture. To study possible epigenetic mechanism controlling activity of Tos17, we subjected three rice lines (the parental line cv. Matsumae and two introgression lines, RZ2 and RZ35) that harbor different copies...

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Veröffentlicht in:	Theoretical and applied genetics 2004-06, Vol.109 (1), p.200-209
Hauptverfasser:	Liu, Z.L, Han, F.P, Tan, M, Shan, X.H, Dong, Y.Z, Wang, X.Z, Fedak, G, Hao, S, Liu, B
Format:	Artikel
Sprache:	eng
Schlagworte:	5-azacytidine Azacitidine - pharmacology Biological and medical sciences Blotting, Northern Blotting, Southern chemical reactions Classical genetics, quantitative genetics, hybrids cytosine Cytosine - metabolism cytosine hypermethylation cytosine hypomethylation DNA Methylation - drug effects DNA Primers DNA Probes drugs Epigenesis, Genetic - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation gene silencing Genetics of eukaryotes. Biological and molecular evolution in vitro regeneration introgression lines lines messenger RNA methylation Oryza - genetics Oryza - metabolism Oryza sativa Pteridophyta, spermatophyta Retroelements - genetics retrotransposons Reverse Transcriptase Polymerase Chain Reaction rice tissue culture transcription (genetics) transcriptional activation transposition (genetics) Vegetals
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