Bi-directional duplex promoters with duplicated enhancers significantly increase transgene expression in grape and tobacco

Novel bi-directional duplex promoters (BDDP) were constructed by placing two identical core promoters divergently on both upstream and downstream sides of their duplicated enhancer elements. Estimates of promoter function were obtained by creating versions of CaMV 35S and CsVMV BDDPs that contained...

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Veröffentlicht in:	Transgenic research 2004-04, Vol.13 (2), p.143-154
Hauptverfasser:	Li, Z.T, Jayasankar, S, Gray, D.J
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium tumefaciens Amino Acid Oxidoreductases - genetics beta-glucuronidase Biological and medical sciences Biotechnology Cassava vein mosaic virus Cauliflower mosaic virus Caulimovirus - genetics enhancer elements Enhancer Elements, Genetic - genetics Fundamental and applied biological sciences. Psychology Gene expression gene expression regulation Gene Expression Regulation, Plant - genetics Genes, Reporter - genetics Genetic engineering Genetic technics genetic transformation Genetic Vectors - genetics Glucuronidase - analysis Glucuronidase - genetics grapes green fluorescent protein Green Fluorescent Proteins - analysis Green Fluorescent Proteins - genetics kanamycin kinase Kanamycin Kinase - genetics kinases Methods. Procedures. Technologies Nicotiana - genetics Nicotiana tabacum plant viruses Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism plasmid vectors promoter regions Promoter Regions, Genetic - genetics Seeds - ultrastructure tobacco transcription (genetics) transgenes Transgenic animals and transgenic plants transgenic plants Vitis - genetics Vitis vinifera
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