Expression of Cathepsin B and L antigen and activity is associated with early colorectal cancer progression
Cathepsin B and Cathepsin L are cysteine proteases important in the process of invasion and metastasis. The aim of our study was to assay antigen and activity levels of these enzymes and to correlate these with established clinical and pathological prognostic parameters including patient survival. 9...
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Veröffentlicht in: | European journal of cancer (1990) 2004-07, Vol.40 (10), p.1610-1616 |
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container_title | European journal of cancer (1990) |
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creator | Troy, A.M Sheahan, K Mulcahy, H.E Duffy, M.J Hyland, J.M.P O'Donoghue, D.P |
description | Cathepsin B and Cathepsin L are cysteine proteases important in the process of invasion and metastasis. The aim of our study was to assay antigen and activity levels of these enzymes and to correlate these with established clinical and pathological prognostic parameters including patient survival. 99 patients undergoing operations for colorectal cancer were included in this study. We quantitated cathepsin B and L levels in matched normal mucosa and cancer samples using an enzyme-linked immunosorbent assay (ELISA) and specific activity assays and expressed the results as tumour/normal ratios. Significant correlations were found between tumour/normal cathepsin B and L antigen and activity ratios. Cathepsin B and L tumour/normal activity ratios were greater than 1 in early stage disease and there were gradual reductions in cathepsin B (
P=0.02) and L (
P=0.03) activity ratios with advancing tumour stage. Survival of patients with potentially curative disease was inversely related to both cathepsin B (
P=0.007) and L (
P=0.001) activity ratio, in addition to cathepsin L antigen ratio (
P=0.008). Our findings suggest that cysteine proteases play an important role in colorectal cancer progression. |
doi_str_mv | 10.1016/j.ejca.2004.03.011 |
format | Article |
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P=0.02) and L (
P=0.03) activity ratios with advancing tumour stage. Survival of patients with potentially curative disease was inversely related to both cathepsin B (
P=0.007) and L (
P=0.001) activity ratio, in addition to cathepsin L antigen ratio (
P=0.008). Our findings suggest that cysteine proteases play an important role in colorectal cancer progression.</description><identifier>ISSN: 0959-8049</identifier><identifier>EISSN: 1879-0852</identifier><identifier>DOI: 10.1016/j.ejca.2004.03.011</identifier><identifier>PMID: 15196548</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Biological and medical sciences ; Cathepsin ; Cathepsin B - metabolism ; Cathepsin L ; Cathepsins - metabolism ; colorectal cancer ; Colorectal Neoplasms - metabolism ; Cysteine Endopeptidases ; Disease Progression ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; Male ; Medical sciences ; Middle Aged ; Neoplasm Proteins - metabolism ; Neoplasm Staging ; Pharmacology. Drug treatments ; Prognosis ; protease ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus ; Survival Analysis ; Tumors ; tumour stage</subject><ispartof>European journal of cancer (1990), 2004-07, Vol.40 (10), p.1610-1616</ispartof><rights>2004 Elsevier Ltd</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-69fb451933c788d918b5f44a1caf8463d8237a6181fa4e6b239099a0226cc2093</citedby><cites>FETCH-LOGICAL-c382t-69fb451933c788d918b5f44a1caf8463d8237a6181fa4e6b239099a0226cc2093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ejca.2004.03.011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15916649$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15196548$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Troy, A.M</creatorcontrib><creatorcontrib>Sheahan, K</creatorcontrib><creatorcontrib>Mulcahy, H.E</creatorcontrib><creatorcontrib>Duffy, M.J</creatorcontrib><creatorcontrib>Hyland, J.M.P</creatorcontrib><creatorcontrib>O'Donoghue, D.P</creatorcontrib><title>Expression of Cathepsin B and L antigen and activity is associated with early colorectal cancer progression</title><title>European journal of cancer (1990)</title><addtitle>Eur J Cancer</addtitle><description>Cathepsin B and Cathepsin L are cysteine proteases important in the process of invasion and metastasis. The aim of our study was to assay antigen and activity levels of these enzymes and to correlate these with established clinical and pathological prognostic parameters including patient survival. 99 patients undergoing operations for colorectal cancer were included in this study. We quantitated cathepsin B and L levels in matched normal mucosa and cancer samples using an enzyme-linked immunosorbent assay (ELISA) and specific activity assays and expressed the results as tumour/normal ratios. Significant correlations were found between tumour/normal cathepsin B and L antigen and activity ratios. Cathepsin B and L tumour/normal activity ratios were greater than 1 in early stage disease and there were gradual reductions in cathepsin B (
P=0.02) and L (
P=0.03) activity ratios with advancing tumour stage. Survival of patients with potentially curative disease was inversely related to both cathepsin B (
P=0.007) and L (
P=0.001) activity ratio, in addition to cathepsin L antigen ratio (
P=0.008). Our findings suggest that cysteine proteases play an important role in colorectal cancer progression.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>Cathepsin</subject><subject>Cathepsin B - metabolism</subject><subject>Cathepsin L</subject><subject>Cathepsins - metabolism</subject><subject>colorectal cancer</subject><subject>Colorectal Neoplasms - metabolism</subject><subject>Cysteine Endopeptidases</subject><subject>Disease Progression</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Neoplasm Staging</subject><subject>Pharmacology. Drug treatments</subject><subject>Prognosis</subject><subject>protease</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</subject><subject>Survival Analysis</subject><subject>Tumors</subject><subject>tumour stage</subject><issn>0959-8049</issn><issn>1879-0852</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhi1ERZfCH-CAfKG3hLHjOLbEha7Kh7RSL3C2Zh2n9ZKNF9tbuv8eh40EJy4zGumZmVcPIW8Y1AyYfL-r3c5izQFEDU0NjD0jK6Y6XYFq-XOyAt3qSoHQl-RlSjsA6JSAF-SStUzLVqgV-XH7dIguJR8mGga6xvzgDslP9Ibi1NNNqdnfu-nPhDb7R59P1CeKKQXrMbue_vL5gTqM44naMIbobMaRWpysi_QQw_3y4BW5GHBM7vXSr8j3T7ff1l-qzd3nr-uPm8o2iudK6mErSsCmsZ1SvWZq2w5CILM4KCGbXvGmQ8kUG1A4ueWNBq0ROJfWctDNFbk-3y2_fx5dymbvk3XjiJMLx2Q6DlxyoQrIz6CNIaXoBnOIfo_xZBiYWbHZmVmxmRUbaExRXJbeLteP273r_64sTgvwbgEwWRyHWET49A-nmZRijvnhzLni4tG7aJL1rkjr_azQ9MH_L8dvehaZ7w</recordid><startdate>20040701</startdate><enddate>20040701</enddate><creator>Troy, A.M</creator><creator>Sheahan, K</creator><creator>Mulcahy, H.E</creator><creator>Duffy, M.J</creator><creator>Hyland, J.M.P</creator><creator>O'Donoghue, D.P</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040701</creationdate><title>Expression of Cathepsin B and L antigen and activity is associated with early colorectal cancer progression</title><author>Troy, A.M ; Sheahan, K ; Mulcahy, H.E ; Duffy, M.J ; Hyland, J.M.P ; O'Donoghue, D.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-69fb451933c788d918b5f44a1caf8463d8237a6181fa4e6b239099a0226cc2093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Cathepsin</topic><topic>Cathepsin B - metabolism</topic><topic>Cathepsin L</topic><topic>Cathepsins - metabolism</topic><topic>colorectal cancer</topic><topic>Colorectal Neoplasms - metabolism</topic><topic>Cysteine Endopeptidases</topic><topic>Disease Progression</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Neoplasm Staging</topic><topic>Pharmacology. Drug treatments</topic><topic>Prognosis</topic><topic>protease</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</topic><topic>Survival Analysis</topic><topic>Tumors</topic><topic>tumour stage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Troy, A.M</creatorcontrib><creatorcontrib>Sheahan, K</creatorcontrib><creatorcontrib>Mulcahy, H.E</creatorcontrib><creatorcontrib>Duffy, M.J</creatorcontrib><creatorcontrib>Hyland, J.M.P</creatorcontrib><creatorcontrib>O'Donoghue, D.P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of cancer (1990)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Troy, A.M</au><au>Sheahan, K</au><au>Mulcahy, H.E</au><au>Duffy, M.J</au><au>Hyland, J.M.P</au><au>O'Donoghue, D.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Cathepsin B and L antigen and activity is associated with early colorectal cancer progression</atitle><jtitle>European journal of cancer (1990)</jtitle><addtitle>Eur J Cancer</addtitle><date>2004-07-01</date><risdate>2004</risdate><volume>40</volume><issue>10</issue><spage>1610</spage><epage>1616</epage><pages>1610-1616</pages><issn>0959-8049</issn><eissn>1879-0852</eissn><abstract>Cathepsin B and Cathepsin L are cysteine proteases important in the process of invasion and metastasis. The aim of our study was to assay antigen and activity levels of these enzymes and to correlate these with established clinical and pathological prognostic parameters including patient survival. 99 patients undergoing operations for colorectal cancer were included in this study. We quantitated cathepsin B and L levels in matched normal mucosa and cancer samples using an enzyme-linked immunosorbent assay (ELISA) and specific activity assays and expressed the results as tumour/normal ratios. Significant correlations were found between tumour/normal cathepsin B and L antigen and activity ratios. Cathepsin B and L tumour/normal activity ratios were greater than 1 in early stage disease and there were gradual reductions in cathepsin B (
P=0.02) and L (
P=0.03) activity ratios with advancing tumour stage. Survival of patients with potentially curative disease was inversely related to both cathepsin B (
P=0.007) and L (
P=0.001) activity ratio, in addition to cathepsin L antigen ratio (
P=0.008). Our findings suggest that cysteine proteases play an important role in colorectal cancer progression.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>15196548</pmid><doi>10.1016/j.ejca.2004.03.011</doi><tpages>7</tpages></addata></record> |
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subjects | Adult Aged Aged, 80 and over Biological and medical sciences Cathepsin Cathepsin B - metabolism Cathepsin L Cathepsins - metabolism colorectal cancer Colorectal Neoplasms - metabolism Cysteine Endopeptidases Disease Progression Female Gastroenterology. Liver. Pancreas. Abdomen Humans Male Medical sciences Middle Aged Neoplasm Proteins - metabolism Neoplasm Staging Pharmacology. Drug treatments Prognosis protease Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Survival Analysis Tumors tumour stage |
title | Expression of Cathepsin B and L antigen and activity is associated with early colorectal cancer progression |
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