Expression of Cathepsin B and L antigen and activity is associated with early colorectal cancer progression

Cathepsin B and Cathepsin L are cysteine proteases important in the process of invasion and metastasis. The aim of our study was to assay antigen and activity levels of these enzymes and to correlate these with established clinical and pathological prognostic parameters including patient survival. 9...

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Veröffentlicht in:European journal of cancer (1990) 2004-07, Vol.40 (10), p.1610-1616
Hauptverfasser: Troy, A.M, Sheahan, K, Mulcahy, H.E, Duffy, M.J, Hyland, J.M.P, O'Donoghue, D.P
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container_end_page 1616
container_issue 10
container_start_page 1610
container_title European journal of cancer (1990)
container_volume 40
creator Troy, A.M
Sheahan, K
Mulcahy, H.E
Duffy, M.J
Hyland, J.M.P
O'Donoghue, D.P
description Cathepsin B and Cathepsin L are cysteine proteases important in the process of invasion and metastasis. The aim of our study was to assay antigen and activity levels of these enzymes and to correlate these with established clinical and pathological prognostic parameters including patient survival. 99 patients undergoing operations for colorectal cancer were included in this study. We quantitated cathepsin B and L levels in matched normal mucosa and cancer samples using an enzyme-linked immunosorbent assay (ELISA) and specific activity assays and expressed the results as tumour/normal ratios. Significant correlations were found between tumour/normal cathepsin B and L antigen and activity ratios. Cathepsin B and L tumour/normal activity ratios were greater than 1 in early stage disease and there were gradual reductions in cathepsin B ( P=0.02) and L ( P=0.03) activity ratios with advancing tumour stage. Survival of patients with potentially curative disease was inversely related to both cathepsin B ( P=0.007) and L ( P=0.001) activity ratio, in addition to cathepsin L antigen ratio ( P=0.008). Our findings suggest that cysteine proteases play an important role in colorectal cancer progression.
doi_str_mv 10.1016/j.ejca.2004.03.011
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The aim of our study was to assay antigen and activity levels of these enzymes and to correlate these with established clinical and pathological prognostic parameters including patient survival. 99 patients undergoing operations for colorectal cancer were included in this study. We quantitated cathepsin B and L levels in matched normal mucosa and cancer samples using an enzyme-linked immunosorbent assay (ELISA) and specific activity assays and expressed the results as tumour/normal ratios. Significant correlations were found between tumour/normal cathepsin B and L antigen and activity ratios. Cathepsin B and L tumour/normal activity ratios were greater than 1 in early stage disease and there were gradual reductions in cathepsin B ( P=0.02) and L ( P=0.03) activity ratios with advancing tumour stage. Survival of patients with potentially curative disease was inversely related to both cathepsin B ( P=0.007) and L ( P=0.001) activity ratio, in addition to cathepsin L antigen ratio ( P=0.008). Our findings suggest that cysteine proteases play an important role in colorectal cancer progression.</description><identifier>ISSN: 0959-8049</identifier><identifier>EISSN: 1879-0852</identifier><identifier>DOI: 10.1016/j.ejca.2004.03.011</identifier><identifier>PMID: 15196548</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Biological and medical sciences ; Cathepsin ; Cathepsin B - metabolism ; Cathepsin L ; Cathepsins - metabolism ; colorectal cancer ; Colorectal Neoplasms - metabolism ; Cysteine Endopeptidases ; Disease Progression ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; Male ; Medical sciences ; Middle Aged ; Neoplasm Proteins - metabolism ; Neoplasm Staging ; Pharmacology. 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source MEDLINE; Elsevier ScienceDirect Journals
subjects Adult
Aged
Aged, 80 and over
Biological and medical sciences
Cathepsin
Cathepsin B - metabolism
Cathepsin L
Cathepsins - metabolism
colorectal cancer
Colorectal Neoplasms - metabolism
Cysteine Endopeptidases
Disease Progression
Female
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Male
Medical sciences
Middle Aged
Neoplasm Proteins - metabolism
Neoplasm Staging
Pharmacology. Drug treatments
Prognosis
protease
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Survival Analysis
Tumors
tumour stage
title Expression of Cathepsin B and L antigen and activity is associated with early colorectal cancer progression
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