Molecular Species of Acylglycerols Incorporating Radiolabeled Fatty Acids from Castor (Ricinus communis L.) Microsomal Incubations
Sixty-one molecular species of triacylglycerols (TAG) and diacylglycerols produced from castor microsomal incubations incorporating six different 14C-labeled fatty acids have been identified and quantified. The preference for incorporation into TAG was in the order ricinoleate > oleate > linol...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2002-08, Vol.50 (18), p.5077-5081 |
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| creator | Lin, Jiann-Tsyh Chen, Jennifer M Liao, Lucy P McKeon, Thomas A |
| description | Sixty-one molecular species of triacylglycerols (TAG) and diacylglycerols produced from castor microsomal incubations incorporating six different 14C-labeled fatty acids have been identified and quantified. The preference for incorporation into TAG was in the order ricinoleate > oleate > linoleate > linolenate > stearate > palmitate. Ricinoleate was the major fatty acid incorporated, whereas stearate, linolenate, and palmitate were incorporated at low levels. Twenty-one molecular species of acylglycerols (HPLC peaks) in castor oil have also been assigned. The levels of TAG in castor oil are RRR (triricinolein) ≫ RR-TAG ≫ R-TAG > no R-TAG. The levels of the molecular species within the groups of RR-TAG, RL-TAG, and LL-TAG individually are ricinoleate > linoleate > oleate > linolenate, stearate, and palmitate. The results of the labeled fatty acid incorporation are consistent with ricinoleate being preferentially driven into TAG and oleate being converted to ricinoleate in castor oil biosynthesis. Keywords: Molecular species; triacylglycerol; diacylglycerol; castor oil; biosynthesis; HPLC; relative retention times; Ricinus communis L. |
| doi_str_mv | 10.1021/jf020454a |
| format | Article |
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Microsomal Incubations</title><source>ACS Publications</source><source>MEDLINE</source><creator>Lin, Jiann-Tsyh ; Chen, Jennifer M ; Liao, Lucy P ; McKeon, Thomas A</creator><creatorcontrib>Lin, Jiann-Tsyh ; Chen, Jennifer M ; Liao, Lucy P ; McKeon, Thomas A</creatorcontrib><description>Sixty-one molecular species of triacylglycerols (TAG) and diacylglycerols produced from castor microsomal incubations incorporating six different 14C-labeled fatty acids have been identified and quantified. The preference for incorporation into TAG was in the order ricinoleate > oleate > linoleate > linolenate > stearate > palmitate. Ricinoleate was the major fatty acid incorporated, whereas stearate, linolenate, and palmitate were incorporated at low levels. Twenty-one molecular species of acylglycerols (HPLC peaks) in castor oil have also been assigned. The levels of TAG in castor oil are RRR (triricinolein) ≫ RR-TAG ≫ R-TAG > no R-TAG. The levels of the molecular species within the groups of RR-TAG, RL-TAG, and LL-TAG individually are ricinoleate > linoleate > oleate > linolenate, stearate, and palmitate. The results of the labeled fatty acid incorporation are consistent with ricinoleate being preferentially driven into TAG and oleate being converted to ricinoleate in castor oil biosynthesis. Keywords: Molecular species; triacylglycerol; diacylglycerol; castor oil; biosynthesis; HPLC; relative retention times; Ricinus communis L.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf020454a</identifier><identifier>PMID: 12188611</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Agronomy. Soil science and plant productions ; Biological and medical sciences ; Carbon Radioisotopes ; Castor Oil - chemistry ; Chromatography, High Pressure Liquid ; Diglycerides - analysis ; Diglycerides - metabolism ; Economic plant physiology ; Fatty Acids - analysis ; Fatty Acids - metabolism ; Fundamental and applied biological sciences. Psychology ; Isotope Labeling ; Microsomes - chemistry ; Nitrogen metabolism and other ones (excepting carbon metabolism) ; Nutrition. Photosynthesis. Respiration. Metabolism ; Oleic Acid - analysis ; Oleic Acid - metabolism ; Ricinoleic Acids - analysis ; Ricinoleic Acids - metabolism ; Ricinus - ultrastructure ; Triglycerides - analysis ; Triglycerides - metabolism</subject><ispartof>Journal of agricultural and food chemistry, 2002-08, Vol.50 (18), p.5077-5081</ispartof><rights>Copyright © 2002 American Chemical Society</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a379t-fbe45512a92d0434b7ca802712fb7b70899066bc20fcaebab69d957d89a172be3</citedby><cites>FETCH-LOGICAL-a379t-fbe45512a92d0434b7ca802712fb7b70899066bc20fcaebab69d957d89a172be3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf020454a$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf020454a$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13877813$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12188611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Jiann-Tsyh</creatorcontrib><creatorcontrib>Chen, Jennifer M</creatorcontrib><creatorcontrib>Liao, Lucy P</creatorcontrib><creatorcontrib>McKeon, Thomas A</creatorcontrib><title>Molecular Species of Acylglycerols Incorporating Radiolabeled Fatty Acids from Castor (Ricinus communis L.) Microsomal Incubations</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Sixty-one molecular species of triacylglycerols (TAG) and diacylglycerols produced from castor microsomal incubations incorporating six different 14C-labeled fatty acids have been identified and quantified. The preference for incorporation into TAG was in the order ricinoleate > oleate > linoleate > linolenate > stearate > palmitate. Ricinoleate was the major fatty acid incorporated, whereas stearate, linolenate, and palmitate were incorporated at low levels. Twenty-one molecular species of acylglycerols (HPLC peaks) in castor oil have also been assigned. The levels of TAG in castor oil are RRR (triricinolein) ≫ RR-TAG ≫ R-TAG > no R-TAG. The levels of the molecular species within the groups of RR-TAG, RL-TAG, and LL-TAG individually are ricinoleate > linoleate > oleate > linolenate, stearate, and palmitate. The results of the labeled fatty acid incorporation are consistent with ricinoleate being preferentially driven into TAG and oleate being converted to ricinoleate in castor oil biosynthesis. Keywords: Molecular species; triacylglycerol; diacylglycerol; castor oil; biosynthesis; HPLC; relative retention times; Ricinus communis L.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Carbon Radioisotopes</subject><subject>Castor Oil - chemistry</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Diglycerides - analysis</subject><subject>Diglycerides - metabolism</subject><subject>Economic plant physiology</subject><subject>Fatty Acids - analysis</subject><subject>Fatty Acids - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isotope Labeling</subject><subject>Microsomes - chemistry</subject><subject>Nitrogen metabolism and other ones (excepting carbon metabolism)</subject><subject>Nutrition. Photosynthesis. Respiration. Metabolism</subject><subject>Oleic Acid - analysis</subject><subject>Oleic Acid - metabolism</subject><subject>Ricinoleic Acids - analysis</subject><subject>Ricinoleic Acids - metabolism</subject><subject>Ricinus - ultrastructure</subject><subject>Triglycerides - analysis</subject><subject>Triglycerides - metabolism</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0E9r2zAYx3ExVtas62FvYOiysR7cSfIfyccS1rWQspGmFHYRj2S5KJOtVI8Ny3WvfA4JzaUnHfThy8OPkI-cXXIm-Ld1ywQrygLekBkvBctKztVbMmPTZ6bKip-S94hrxpgqJXtHTrngSlWcz8i_uxicHQMker9x1juksaVXdhuewta6FAPS297GtIkJBt8_0SU0PgYwLriGXsMwbCfuG6Rtih2dAw4x0a9Lb30_IrWx68beI11cXtA7b1PE2EHYNUczBWOPH8hJCwHd-eE9Iw_X31fzm2zx88ft_GqRQS7rIWuNK8qSC6hFw4q8MNKCYkJy0RppJFN1zarKWMFaC86AqeqmLmWjauBSGJefkS_77ibF59HhoDuP1oUAvYsjaimYEFNnghd7uLsWk2v1JvkO0lZzpneD65fBJ_vpEB1N55qjPCw8gc8HAGghtAl66_HociWl4vnksr3zOLi_L_-Q_uhK5rLUq1_3uuK_V2L5eKMfj12wqNdxTP203SsH_gcAcaS8</recordid><startdate>20020828</startdate><enddate>20020828</enddate><creator>Lin, Jiann-Tsyh</creator><creator>Chen, Jennifer M</creator><creator>Liao, Lucy P</creator><creator>McKeon, Thomas A</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020828</creationdate><title>Molecular Species of Acylglycerols Incorporating Radiolabeled Fatty Acids from Castor (Ricinus communis L.) Microsomal Incubations</title><author>Lin, Jiann-Tsyh ; Chen, Jennifer M ; Liao, Lucy P ; McKeon, Thomas A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a379t-fbe45512a92d0434b7ca802712fb7b70899066bc20fcaebab69d957d89a172be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>Carbon Radioisotopes</topic><topic>Castor Oil - chemistry</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Diglycerides - analysis</topic><topic>Diglycerides - metabolism</topic><topic>Economic plant physiology</topic><topic>Fatty Acids - analysis</topic><topic>Fatty Acids - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isotope Labeling</topic><topic>Microsomes - chemistry</topic><topic>Nitrogen metabolism and other ones (excepting carbon metabolism)</topic><topic>Nutrition. Photosynthesis. Respiration. Metabolism</topic><topic>Oleic Acid - analysis</topic><topic>Oleic Acid - metabolism</topic><topic>Ricinoleic Acids - analysis</topic><topic>Ricinoleic Acids - metabolism</topic><topic>Ricinus - ultrastructure</topic><topic>Triglycerides - analysis</topic><topic>Triglycerides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Jiann-Tsyh</creatorcontrib><creatorcontrib>Chen, Jennifer M</creatorcontrib><creatorcontrib>Liao, Lucy P</creatorcontrib><creatorcontrib>McKeon, Thomas A</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Jiann-Tsyh</au><au>Chen, Jennifer M</au><au>Liao, Lucy P</au><au>McKeon, Thomas A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Species of Acylglycerols Incorporating Radiolabeled Fatty Acids from Castor (Ricinus communis L.) Microsomal Incubations</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2002-08-28</date><risdate>2002</risdate><volume>50</volume><issue>18</issue><spage>5077</spage><epage>5081</epage><pages>5077-5081</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Sixty-one molecular species of triacylglycerols (TAG) and diacylglycerols produced from castor microsomal incubations incorporating six different 14C-labeled fatty acids have been identified and quantified. The preference for incorporation into TAG was in the order ricinoleate > oleate > linoleate > linolenate > stearate > palmitate. Ricinoleate was the major fatty acid incorporated, whereas stearate, linolenate, and palmitate were incorporated at low levels. Twenty-one molecular species of acylglycerols (HPLC peaks) in castor oil have also been assigned. The levels of TAG in castor oil are RRR (triricinolein) ≫ RR-TAG ≫ R-TAG > no R-TAG. The levels of the molecular species within the groups of RR-TAG, RL-TAG, and LL-TAG individually are ricinoleate > linoleate > oleate > linolenate, stearate, and palmitate. The results of the labeled fatty acid incorporation are consistent with ricinoleate being preferentially driven into TAG and oleate being converted to ricinoleate in castor oil biosynthesis. Keywords: Molecular species; triacylglycerol; diacylglycerol; castor oil; biosynthesis; HPLC; relative retention times; Ricinus communis L.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>12188611</pmid><doi>10.1021/jf020454a</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 2002-08, Vol.50 (18), p.5077-5081 |
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| subjects | Agronomy. Soil science and plant productions Biological and medical sciences Carbon Radioisotopes Castor Oil - chemistry Chromatography, High Pressure Liquid Diglycerides - analysis Diglycerides - metabolism Economic plant physiology Fatty Acids - analysis Fatty Acids - metabolism Fundamental and applied biological sciences. Psychology Isotope Labeling Microsomes - chemistry Nitrogen metabolism and other ones (excepting carbon metabolism) Nutrition. Photosynthesis. Respiration. Metabolism Oleic Acid - analysis Oleic Acid - metabolism Ricinoleic Acids - analysis Ricinoleic Acids - metabolism Ricinus - ultrastructure Triglycerides - analysis Triglycerides - metabolism |
| title | Molecular Species of Acylglycerols Incorporating Radiolabeled Fatty Acids from Castor (Ricinus communis L.) Microsomal Incubations |
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