Characteristics and transplantation of the porcine neonatal pancreatic cell clusters isolated from 1- to 3-Day-Old versus 1-Month-Old pigs

Porcine neonatal pancreatic cell clusters (NPCCs) isolated from 1- to 3-day-old pigs (I-A) cured diabetic nude mice within 8 weeks after transplantation. To shorten the latent period between transplantation and reversal of hyperglycemia, we studied NPCCs isolated from 1-month-old pigs (I-B). One- to...

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Veröffentlicht in:Transplantation proceedings 2004-05, Vol.36 (4), p.1203-1205
Hauptverfasser: Juang, J.-H, Hsu, B.R.-S, Kuo, C.-H, Yao, N.-K
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Hsu, B.R.-S
Kuo, C.-H
Yao, N.-K
description Porcine neonatal pancreatic cell clusters (NPCCs) isolated from 1- to 3-day-old pigs (I-A) cured diabetic nude mice within 8 weeks after transplantation. To shorten the latent period between transplantation and reversal of hyperglycemia, we studied NPCCs isolated from 1-month-old pigs (I-B). One- to 3-day-old or 1-month-old pig pancreata were cut into fragments, digested by collagenase, and then studied for islet characteristics. In addition, 300 cultured NPCCs were transplanted under kidney capsule of nondiabetic nude mice. At 1 and 3 months after transplantation, the grafts were removed to measure the insulin content and β-cell mass. Immediately after isolation, I-B was larger than I-A (0.211 ± 0.006 vs 0.189 ± 0.003 mm 2, P = .0003) and after a 6-day culture period, I-B contained more insulin than I-A (6.8 ± 1.4 vs 2.3 ± 0.2 μg/150 NPCCs, P = .02). However, the stimulation indices of I-A and I-B during static incubation with 500 mg/dL glucose (26.5 ± 3.2 vs 23.9 ± 1.7) or 500 mg/dL glucose plus 50 mol/L IBMX (41.9 ± 4.4 vs 62.2 ± 14.0) were not significantly different. Furthermore, neither I-A nor I-B showed first or second phase insulin secretion during sequential perifusion with 100 or 300 mg/dL glucose. In nondiabetic recipients, the insulin content of the graft at 1 month after transplantation was 0.3 ± 0.0 and 0.3 ± 0.1 μg, and the β-cell mass of the graft at 3 months was 0.069 ± 0.022 and 0.067 ± 0.023 mg in mice receiving I-A or I-B, respectively ( P > .05). Our data indicate NPCCs isolated from 1- to 3-day-old and 1-month-old pigs have different characteristics but similar transplantation effects.
doi_str_mv 10.1016/j.transproceed.2004.04.038
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To shorten the latent period between transplantation and reversal of hyperglycemia, we studied NPCCs isolated from 1-month-old pigs (I-B). One- to 3-day-old or 1-month-old pig pancreata were cut into fragments, digested by collagenase, and then studied for islet characteristics. In addition, 300 cultured NPCCs were transplanted under kidney capsule of nondiabetic nude mice. At 1 and 3 months after transplantation, the grafts were removed to measure the insulin content and β-cell mass. Immediately after isolation, I-B was larger than I-A (0.211 ± 0.006 vs 0.189 ± 0.003 mm 2, P = .0003) and after a 6-day culture period, I-B contained more insulin than I-A (6.8 ± 1.4 vs 2.3 ± 0.2 μg/150 NPCCs, P = .02). However, the stimulation indices of I-A and I-B during static incubation with 500 mg/dL glucose (26.5 ± 3.2 vs 23.9 ± 1.7) or 500 mg/dL glucose plus 50 mol/L IBMX (41.9 ± 4.4 vs 62.2 ± 14.0) were not significantly different. Furthermore, neither I-A nor I-B showed first or second phase insulin secretion during sequential perifusion with 100 or 300 mg/dL glucose. In nondiabetic recipients, the insulin content of the graft at 1 month after transplantation was 0.3 ± 0.0 and 0.3 ± 0.1 μg, and the β-cell mass of the graft at 3 months was 0.069 ± 0.022 and 0.067 ± 0.023 mg in mice receiving I-A or I-B, respectively ( P &gt; .05). 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To shorten the latent period between transplantation and reversal of hyperglycemia, we studied NPCCs isolated from 1-month-old pigs (I-B). One- to 3-day-old or 1-month-old pig pancreata were cut into fragments, digested by collagenase, and then studied for islet characteristics. In addition, 300 cultured NPCCs were transplanted under kidney capsule of nondiabetic nude mice. At 1 and 3 months after transplantation, the grafts were removed to measure the insulin content and β-cell mass. Immediately after isolation, I-B was larger than I-A (0.211 ± 0.006 vs 0.189 ± 0.003 mm 2, P = .0003) and after a 6-day culture period, I-B contained more insulin than I-A (6.8 ± 1.4 vs 2.3 ± 0.2 μg/150 NPCCs, P = .02). However, the stimulation indices of I-A and I-B during static incubation with 500 mg/dL glucose (26.5 ± 3.2 vs 23.9 ± 1.7) or 500 mg/dL glucose plus 50 mol/L IBMX (41.9 ± 4.4 vs 62.2 ± 14.0) were not significantly different. Furthermore, neither I-A nor I-B showed first or second phase insulin secretion during sequential perifusion with 100 or 300 mg/dL glucose. In nondiabetic recipients, the insulin content of the graft at 1 month after transplantation was 0.3 ± 0.0 and 0.3 ± 0.1 μg, and the β-cell mass of the graft at 3 months was 0.069 ± 0.022 and 0.067 ± 0.023 mg in mice receiving I-A or I-B, respectively ( P &gt; .05). Our data indicate NPCCs isolated from 1- to 3-day-old and 1-month-old pigs have different characteristics but similar transplantation effects.</description><subject>Aging - physiology</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biological and medical sciences</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Glucagon - analysis</subject><subject>Insulin - analysis</subject><subject>Islets of Langerhans - cytology</subject><subject>Islets of Langerhans Transplantation - methods</subject><subject>Islets of Langerhans Transplantation - pathology</subject><subject>Islets of Langerhans Transplantation - physiology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Pancreatic Polypeptide - analysis</subject><subject>Somatostatin - analysis</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. 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Psychology</topic><topic>Fundamental immunology</topic><topic>Glucagon - analysis</topic><topic>Insulin - analysis</topic><topic>Islets of Langerhans - cytology</topic><topic>Islets of Langerhans Transplantation - methods</topic><topic>Islets of Langerhans Transplantation - pathology</topic><topic>Islets of Langerhans Transplantation - physiology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Pancreatic Polypeptide - analysis</topic><topic>Somatostatin - analysis</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Swine</topic><topic>Tissue, organ and graft immunology</topic><topic>Transplantation, Heterologous - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Juang, J.-H</creatorcontrib><creatorcontrib>Hsu, B.R.-S</creatorcontrib><creatorcontrib>Kuo, C.-H</creatorcontrib><creatorcontrib>Yao, N.-K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transplantation proceedings</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Juang, J.-H</au><au>Hsu, B.R.-S</au><au>Kuo, C.-H</au><au>Yao, N.-K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characteristics and transplantation of the porcine neonatal pancreatic cell clusters isolated from 1- to 3-Day-Old versus 1-Month-Old pigs</atitle><jtitle>Transplantation proceedings</jtitle><addtitle>Transplant Proc</addtitle><date>2004-05-01</date><risdate>2004</risdate><volume>36</volume><issue>4</issue><spage>1203</spage><epage>1205</epage><pages>1203-1205</pages><issn>0041-1345</issn><eissn>1873-2623</eissn><coden>TRPPA8</coden><abstract>Porcine neonatal pancreatic cell clusters (NPCCs) isolated from 1- to 3-day-old pigs (I-A) cured diabetic nude mice within 8 weeks after transplantation. To shorten the latent period between transplantation and reversal of hyperglycemia, we studied NPCCs isolated from 1-month-old pigs (I-B). One- to 3-day-old or 1-month-old pig pancreata were cut into fragments, digested by collagenase, and then studied for islet characteristics. In addition, 300 cultured NPCCs were transplanted under kidney capsule of nondiabetic nude mice. At 1 and 3 months after transplantation, the grafts were removed to measure the insulin content and β-cell mass. Immediately after isolation, I-B was larger than I-A (0.211 ± 0.006 vs 0.189 ± 0.003 mm 2, P = .0003) and after a 6-day culture period, I-B contained more insulin than I-A (6.8 ± 1.4 vs 2.3 ± 0.2 μg/150 NPCCs, P = .02). However, the stimulation indices of I-A and I-B during static incubation with 500 mg/dL glucose (26.5 ± 3.2 vs 23.9 ± 1.7) or 500 mg/dL glucose plus 50 mol/L IBMX (41.9 ± 4.4 vs 62.2 ± 14.0) were not significantly different. Furthermore, neither I-A nor I-B showed first or second phase insulin secretion during sequential perifusion with 100 or 300 mg/dL glucose. In nondiabetic recipients, the insulin content of the graft at 1 month after transplantation was 0.3 ± 0.0 and 0.3 ± 0.1 μg, and the β-cell mass of the graft at 3 months was 0.069 ± 0.022 and 0.067 ± 0.023 mg in mice receiving I-A or I-B, respectively ( P &gt; .05). Our data indicate NPCCs isolated from 1- to 3-day-old and 1-month-old pigs have different characteristics but similar transplantation effects.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>15194417</pmid><doi>10.1016/j.transproceed.2004.04.038</doi><tpages>3</tpages></addata></record>
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subjects Aging - physiology
Animals
Animals, Newborn
Biological and medical sciences
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Glucagon - analysis
Insulin - analysis
Islets of Langerhans - cytology
Islets of Langerhans Transplantation - methods
Islets of Langerhans Transplantation - pathology
Islets of Langerhans Transplantation - physiology
Male
Medical sciences
Mice
Mice, Nude
Pancreatic Polypeptide - analysis
Somatostatin - analysis
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Swine
Tissue, organ and graft immunology
Transplantation, Heterologous - methods
title Characteristics and transplantation of the porcine neonatal pancreatic cell clusters isolated from 1- to 3-Day-Old versus 1-Month-Old pigs
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