Characteristics and transplantation of the porcine neonatal pancreatic cell clusters isolated from 1- to 3-Day-Old versus 1-Month-Old pigs
Porcine neonatal pancreatic cell clusters (NPCCs) isolated from 1- to 3-day-old pigs (I-A) cured diabetic nude mice within 8 weeks after transplantation. To shorten the latent period between transplantation and reversal of hyperglycemia, we studied NPCCs isolated from 1-month-old pigs (I-B). One- to...
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description | Porcine neonatal pancreatic cell clusters (NPCCs) isolated from 1- to 3-day-old pigs (I-A) cured diabetic nude mice within 8 weeks after transplantation. To shorten the latent period between transplantation and reversal of hyperglycemia, we studied NPCCs isolated from 1-month-old pigs (I-B). One- to 3-day-old or 1-month-old pig pancreata were cut into fragments, digested by collagenase, and then studied for islet characteristics. In addition, 300 cultured NPCCs were transplanted under kidney capsule of nondiabetic nude mice. At 1 and 3 months after transplantation, the grafts were removed to measure the insulin content and β-cell mass. Immediately after isolation, I-B was larger than I-A (0.211 ± 0.006 vs 0.189 ± 0.003 mm
2,
P = .0003) and after a 6-day culture period, I-B contained more insulin than I-A (6.8 ± 1.4 vs 2.3 ± 0.2 μg/150 NPCCs,
P = .02). However, the stimulation indices of I-A and I-B during static incubation with 500 mg/dL glucose (26.5 ± 3.2 vs 23.9 ± 1.7) or 500 mg/dL glucose plus 50 mol/L IBMX (41.9 ± 4.4 vs 62.2 ± 14.0) were not significantly different. Furthermore, neither I-A nor I-B showed first or second phase insulin secretion during sequential perifusion with 100 or 300 mg/dL glucose. In nondiabetic recipients, the insulin content of the graft at 1 month after transplantation was 0.3 ± 0.0 and 0.3 ± 0.1 μg, and the β-cell mass of the graft at 3 months was 0.069 ± 0.022 and 0.067 ± 0.023 mg in mice receiving I-A or I-B, respectively (
P > .05). Our data indicate NPCCs isolated from 1- to 3-day-old and 1-month-old pigs have different characteristics but similar transplantation effects. |
doi_str_mv | 10.1016/j.transproceed.2004.04.038 |
format | Article |
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2,
P = .0003) and after a 6-day culture period, I-B contained more insulin than I-A (6.8 ± 1.4 vs 2.3 ± 0.2 μg/150 NPCCs,
P = .02). However, the stimulation indices of I-A and I-B during static incubation with 500 mg/dL glucose (26.5 ± 3.2 vs 23.9 ± 1.7) or 500 mg/dL glucose plus 50 mol/L IBMX (41.9 ± 4.4 vs 62.2 ± 14.0) were not significantly different. Furthermore, neither I-A nor I-B showed first or second phase insulin secretion during sequential perifusion with 100 or 300 mg/dL glucose. In nondiabetic recipients, the insulin content of the graft at 1 month after transplantation was 0.3 ± 0.0 and 0.3 ± 0.1 μg, and the β-cell mass of the graft at 3 months was 0.069 ± 0.022 and 0.067 ± 0.023 mg in mice receiving I-A or I-B, respectively (
P > .05). Our data indicate NPCCs isolated from 1- to 3-day-old and 1-month-old pigs have different characteristics but similar transplantation effects.</description><identifier>ISSN: 0041-1345</identifier><identifier>EISSN: 1873-2623</identifier><identifier>DOI: 10.1016/j.transproceed.2004.04.038</identifier><identifier>PMID: 15194417</identifier><identifier>CODEN: TRPPA8</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Aging - physiology ; Animals ; Animals, Newborn ; Biological and medical sciences ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Glucagon - analysis ; Insulin - analysis ; Islets of Langerhans - cytology ; Islets of Langerhans Transplantation - methods ; Islets of Langerhans Transplantation - pathology ; Islets of Langerhans Transplantation - physiology ; Male ; Medical sciences ; Mice ; Mice, Nude ; Pancreatic Polypeptide - analysis ; Somatostatin - analysis ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Swine ; Tissue, organ and graft immunology ; Transplantation, Heterologous - methods</subject><ispartof>Transplantation proceedings, 2004-05, Vol.36 (4), p.1203-1205</ispartof><rights>2004 Elsevier Inc.</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-9ba99a2b250c4ec860c05c86b08d51cc1a5b0740904acc1ff118c42aae75b4063</citedby><cites>FETCH-LOGICAL-c406t-9ba99a2b250c4ec860c05c86b08d51cc1a5b0740904acc1ff118c42aae75b4063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.transproceed.2004.04.038$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15870734$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15194417$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Juang, J.-H</creatorcontrib><creatorcontrib>Hsu, B.R.-S</creatorcontrib><creatorcontrib>Kuo, C.-H</creatorcontrib><creatorcontrib>Yao, N.-K</creatorcontrib><title>Characteristics and transplantation of the porcine neonatal pancreatic cell clusters isolated from 1- to 3-Day-Old versus 1-Month-Old pigs</title><title>Transplantation proceedings</title><addtitle>Transplant Proc</addtitle><description>Porcine neonatal pancreatic cell clusters (NPCCs) isolated from 1- to 3-day-old pigs (I-A) cured diabetic nude mice within 8 weeks after transplantation. To shorten the latent period between transplantation and reversal of hyperglycemia, we studied NPCCs isolated from 1-month-old pigs (I-B). One- to 3-day-old or 1-month-old pig pancreata were cut into fragments, digested by collagenase, and then studied for islet characteristics. In addition, 300 cultured NPCCs were transplanted under kidney capsule of nondiabetic nude mice. At 1 and 3 months after transplantation, the grafts were removed to measure the insulin content and β-cell mass. Immediately after isolation, I-B was larger than I-A (0.211 ± 0.006 vs 0.189 ± 0.003 mm
2,
P = .0003) and after a 6-day culture period, I-B contained more insulin than I-A (6.8 ± 1.4 vs 2.3 ± 0.2 μg/150 NPCCs,
P = .02). However, the stimulation indices of I-A and I-B during static incubation with 500 mg/dL glucose (26.5 ± 3.2 vs 23.9 ± 1.7) or 500 mg/dL glucose plus 50 mol/L IBMX (41.9 ± 4.4 vs 62.2 ± 14.0) were not significantly different. Furthermore, neither I-A nor I-B showed first or second phase insulin secretion during sequential perifusion with 100 or 300 mg/dL glucose. In nondiabetic recipients, the insulin content of the graft at 1 month after transplantation was 0.3 ± 0.0 and 0.3 ± 0.1 μg, and the β-cell mass of the graft at 3 months was 0.069 ± 0.022 and 0.067 ± 0.023 mg in mice receiving I-A or I-B, respectively (
P > .05). Our data indicate NPCCs isolated from 1- to 3-day-old and 1-month-old pigs have different characteristics but similar transplantation effects.</description><subject>Aging - physiology</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biological and medical sciences</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Glucagon - analysis</subject><subject>Insulin - analysis</subject><subject>Islets of Langerhans - cytology</subject><subject>Islets of Langerhans Transplantation - methods</subject><subject>Islets of Langerhans Transplantation - pathology</subject><subject>Islets of Langerhans Transplantation - physiology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Pancreatic Polypeptide - analysis</subject><subject>Somatostatin - analysis</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Swine</subject><subject>Tissue, organ and graft immunology</subject><subject>Transplantation, Heterologous - methods</subject><issn>0041-1345</issn><issn>1873-2623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkVFv1DAMxyPExG6Dr4AiJHjrLUnTa8sbusFA2rQXeI5cN-Vy6iUlTiftK-xTL-VOaI9IlizbP__j2Ix9kGIthdxc7dcpgqcpBrS2Xysh9HqxsnnFVrKpy0JtVPmarXJBFrLU1Tm7INqLHCtdvmHnspKt1rJesaftDiJgstFRckgcfM-P8iP4BMkFz8PA087yKUR03nJvg4cEI5_AY7SZQY52HDmOM2Ul4o7CCMn2fIjhwGXBU-BlcQ2Pxf3Y84eMzJTTd8Gn3d_U5H7TW3Y2wEj23clfsl_fvv7cfi9u729-bL_cFqjFJhVtB20LqlOVQG2x2QgUVXadaPpKIkqoOlFr0QoNORoGKRvUCsDWVZcVykv26aibF_hntpTMwdEyP-SPzWRqJZRUtc7g5yOIMRBFO5gpugPERyOFWS5h9ublJcxyCbNY2eTm96dX5u6Qa_9aT6vPwMcTAIQwDlkIHb3gmlrU5TLF9ZGzeScPzkZD6KxH27toMZk-uP-Z5xm6JK_a</recordid><startdate>20040501</startdate><enddate>20040501</enddate><creator>Juang, J.-H</creator><creator>Hsu, B.R.-S</creator><creator>Kuo, C.-H</creator><creator>Yao, N.-K</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040501</creationdate><title>Characteristics and transplantation of the porcine neonatal pancreatic cell clusters isolated from 1- to 3-Day-Old versus 1-Month-Old pigs</title><author>Juang, J.-H ; Hsu, B.R.-S ; Kuo, C.-H ; Yao, N.-K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-9ba99a2b250c4ec860c05c86b08d51cc1a5b0740904acc1ff118c42aae75b4063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Aging - physiology</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Biological and medical sciences</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Glucagon - analysis</topic><topic>Insulin - analysis</topic><topic>Islets of Langerhans - cytology</topic><topic>Islets of Langerhans Transplantation - methods</topic><topic>Islets of Langerhans Transplantation - pathology</topic><topic>Islets of Langerhans Transplantation - physiology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Pancreatic Polypeptide - analysis</topic><topic>Somatostatin - analysis</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Swine</topic><topic>Tissue, organ and graft immunology</topic><topic>Transplantation, Heterologous - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Juang, J.-H</creatorcontrib><creatorcontrib>Hsu, B.R.-S</creatorcontrib><creatorcontrib>Kuo, C.-H</creatorcontrib><creatorcontrib>Yao, N.-K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transplantation proceedings</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Juang, J.-H</au><au>Hsu, B.R.-S</au><au>Kuo, C.-H</au><au>Yao, N.-K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characteristics and transplantation of the porcine neonatal pancreatic cell clusters isolated from 1- to 3-Day-Old versus 1-Month-Old pigs</atitle><jtitle>Transplantation proceedings</jtitle><addtitle>Transplant Proc</addtitle><date>2004-05-01</date><risdate>2004</risdate><volume>36</volume><issue>4</issue><spage>1203</spage><epage>1205</epage><pages>1203-1205</pages><issn>0041-1345</issn><eissn>1873-2623</eissn><coden>TRPPA8</coden><abstract>Porcine neonatal pancreatic cell clusters (NPCCs) isolated from 1- to 3-day-old pigs (I-A) cured diabetic nude mice within 8 weeks after transplantation. To shorten the latent period between transplantation and reversal of hyperglycemia, we studied NPCCs isolated from 1-month-old pigs (I-B). One- to 3-day-old or 1-month-old pig pancreata were cut into fragments, digested by collagenase, and then studied for islet characteristics. In addition, 300 cultured NPCCs were transplanted under kidney capsule of nondiabetic nude mice. At 1 and 3 months after transplantation, the grafts were removed to measure the insulin content and β-cell mass. Immediately after isolation, I-B was larger than I-A (0.211 ± 0.006 vs 0.189 ± 0.003 mm
2,
P = .0003) and after a 6-day culture period, I-B contained more insulin than I-A (6.8 ± 1.4 vs 2.3 ± 0.2 μg/150 NPCCs,
P = .02). However, the stimulation indices of I-A and I-B during static incubation with 500 mg/dL glucose (26.5 ± 3.2 vs 23.9 ± 1.7) or 500 mg/dL glucose plus 50 mol/L IBMX (41.9 ± 4.4 vs 62.2 ± 14.0) were not significantly different. Furthermore, neither I-A nor I-B showed first or second phase insulin secretion during sequential perifusion with 100 or 300 mg/dL glucose. In nondiabetic recipients, the insulin content of the graft at 1 month after transplantation was 0.3 ± 0.0 and 0.3 ± 0.1 μg, and the β-cell mass of the graft at 3 months was 0.069 ± 0.022 and 0.067 ± 0.023 mg in mice receiving I-A or I-B, respectively (
P > .05). Our data indicate NPCCs isolated from 1- to 3-day-old and 1-month-old pigs have different characteristics but similar transplantation effects.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>15194417</pmid><doi>10.1016/j.transproceed.2004.04.038</doi><tpages>3</tpages></addata></record> |
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subjects | Aging - physiology Animals Animals, Newborn Biological and medical sciences Female Fundamental and applied biological sciences. Psychology Fundamental immunology Glucagon - analysis Insulin - analysis Islets of Langerhans - cytology Islets of Langerhans Transplantation - methods Islets of Langerhans Transplantation - pathology Islets of Langerhans Transplantation - physiology Male Medical sciences Mice Mice, Nude Pancreatic Polypeptide - analysis Somatostatin - analysis Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Swine Tissue, organ and graft immunology Transplantation, Heterologous - methods |
title | Characteristics and transplantation of the porcine neonatal pancreatic cell clusters isolated from 1- to 3-Day-Old versus 1-Month-Old pigs |
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