New Rapid Enzyme-Linked Immunosorbent Assay to Detect Antibodies against Bacterial Surface Antigens Using Filtration Plates
An easy and rapid ELISA system, Filtration ELISA, to detect antibodies against bacterial cell surface antigens was developed using a 96-well filtration plate fitted with a 0.22 μm membrane (MultiScreen®-GV, Millipore). Bacterial whole cells were used as antigens without fixing the cells with formali...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2002, Vol.25(8), pp.986-990 |
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| creator | Itoh, Saori Kariya, Masako Nagano, Keiji Yokoyama, Shin-ichiro Fukao, Toshio Yamazaki, Yoshihisa Mori, Hiroshi |
| description | An easy and rapid ELISA system, Filtration ELISA, to detect antibodies against bacterial cell surface antigens was developed using a 96-well filtration plate fitted with a 0.22 μm membrane (MultiScreen®-GV, Millipore). Bacterial whole cells were used as antigens without fixing the cells with formalin etc. The whole cell antigens were washed by vacuum filtration through a filter and resuspended in washing buffer. Assay reactions could be done in the wells without losing the solution. The technique was established using antisera of mice immunized with Escherichia coli, and then evaluated by assaying antibodies to Shiga-toxin producing E. coli O157:H7 (STEC), Staphylococcus aureus and Lactobacillus acidophilus in fecal extracts of 157 children who had eaten school lunches contaminated with STEC in comparison with 25 age-matched control children. The lunch group showed significantly higher IgA antibody titers against STEC than the control group (p |
| doi_str_mv | 10.1248/bpb.25.986 |
| format | Article |
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Bacterial whole cells were used as antigens without fixing the cells with formalin etc. The whole cell antigens were washed by vacuum filtration through a filter and resuspended in washing buffer. Assay reactions could be done in the wells without losing the solution. The technique was established using antisera of mice immunized with Escherichia coli, and then evaluated by assaying antibodies to Shiga-toxin producing E. coli O157:H7 (STEC), Staphylococcus aureus and Lactobacillus acidophilus in fecal extracts of 157 children who had eaten school lunches contaminated with STEC in comparison with 25 age-matched control children. The lunch group showed significantly higher IgA antibody titers against STEC than the control group (p<0.0005), but not against L. acidphilus. The results indicate that Filtration ELISA is a quantitative and specific technique for measuring antibodies against antigens on the surface of bacteria without extracting antigens from the bacteria. This technique is widely applicable to the assay of antibodies in various samples including serum and fecal extract against various kinds of bacteria.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.25.986</identifier><identifier>PMID: 12186431</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>Animals ; Antibodies - analysis ; antibody ; Antigens, Bacterial - analysis ; Antigens, Surface - analysis ; bacterial surface antigen ; Biological and medical sciences ; Biotechnology ; Child ; ELISA ; Enzyme-Linked Immunosorbent Assay - instrumentation ; Enzyme-Linked Immunosorbent Assay - methods ; Escherichia coli - isolation & purification ; feces ; Filtration - instrumentation ; Filtration - methods ; Food industries ; Food microbiology ; Fundamental and applied biological sciences. Psychology ; Genetic technics applied to diagnosis ; Health. 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Bacterial whole cells were used as antigens without fixing the cells with formalin etc. The whole cell antigens were washed by vacuum filtration through a filter and resuspended in washing buffer. Assay reactions could be done in the wells without losing the solution. The technique was established using antisera of mice immunized with Escherichia coli, and then evaluated by assaying antibodies to Shiga-toxin producing E. coli O157:H7 (STEC), Staphylococcus aureus and Lactobacillus acidophilus in fecal extracts of 157 children who had eaten school lunches contaminated with STEC in comparison with 25 age-matched control children. The lunch group showed significantly higher IgA antibody titers against STEC than the control group (p<0.0005), but not against L. acidphilus. The results indicate that Filtration ELISA is a quantitative and specific technique for measuring antibodies against antigens on the surface of bacteria without extracting antigens from the bacteria. This technique is widely applicable to the assay of antibodies in various samples including serum and fecal extract against various kinds of bacteria.</description><subject>Animals</subject><subject>Antibodies - analysis</subject><subject>antibody</subject><subject>Antigens, Bacterial - analysis</subject><subject>Antigens, Surface - analysis</subject><subject>bacterial surface antigen</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Child</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - instrumentation</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Escherichia coli - isolation & purification</subject><subject>feces</subject><subject>Filtration - instrumentation</subject><subject>Filtration - methods</subject><subject>Food industries</subject><subject>Food microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic technics applied to diagnosis</subject><subject>Health. Pharmaceutical industry</subject><subject>Humans</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Mice</subject><subject>O157</subject><subject>Shiga-toxin producing Escherichia coli</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0d-L1DAQB_AgireevvgHSED0QeiaSZs2fZLzvNODRUW95zJNp2vWNl2TFFn958266x344kt-kA8zTL6MPQaxBFnol-22XUq1rHV5hy0gL6pMSVB32ULUoLMSlD5hD0LYCCEqIfP77AQk6LLIYcF-vacf_BNubccv3M_dSNnKum_U8atxnN0UJt-Si_wsBNzxOPE3FMmku4u2nTpLgeMarQuRv0YTyVsc-OfZ92joD1qTC_w6WLfml3aIHqOdHP84YKTwkN3rcQj06LifsuvLiy_n77LVh7dX52erzJQAMdNGG9X3ggS0qEAa01eCFNZKpgF7UYveVLrstCmNhCpvFagyb8s6nRQazE_Z80PdrZ--zxRiM9pgaBjQ0TSHppICaimq_8L0aaJSsIdP_4GbafYuDdFAUdRQ5yWIpF4clPFTCJ76ZuvtiH7XgGj2yTUpuUaqJiWX8JNjybkdqbulx6gSeHYEGAwOvUdnbLh1udaigL17dXCbEHFNNwB9tGagvz31YUmtb17MV_QNufw37gW33g</recordid><startdate>20020801</startdate><enddate>20020801</enddate><creator>Itoh, Saori</creator><creator>Kariya, Masako</creator><creator>Nagano, Keiji</creator><creator>Yokoyama, Shin-ichiro</creator><creator>Fukao, Toshio</creator><creator>Yamazaki, Yoshihisa</creator><creator>Mori, Hiroshi</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7T7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20020801</creationdate><title>New Rapid Enzyme-Linked Immunosorbent Assay to Detect Antibodies against Bacterial Surface Antigens Using Filtration Plates</title><author>Itoh, Saori ; Kariya, Masako ; Nagano, Keiji ; Yokoyama, Shin-ichiro ; Fukao, Toshio ; Yamazaki, Yoshihisa ; Mori, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c611t-8c8c5ff0e01ba512ccf70e5a952091f090fc786d8c6c2173b51563b693b55aca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Antibodies - analysis</topic><topic>antibody</topic><topic>Antigens, Bacterial - analysis</topic><topic>Antigens, Surface - analysis</topic><topic>bacterial surface antigen</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Child</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay - instrumentation</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Escherichia coli - isolation & purification</topic><topic>feces</topic><topic>Filtration - instrumentation</topic><topic>Filtration - methods</topic><topic>Food industries</topic><topic>Food microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic technics applied to diagnosis</topic><topic>Health. Pharmaceutical industry</topic><topic>Humans</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Mice</topic><topic>O157</topic><topic>Shiga-toxin producing Escherichia coli</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Itoh, Saori</creatorcontrib><creatorcontrib>Kariya, Masako</creatorcontrib><creatorcontrib>Nagano, Keiji</creatorcontrib><creatorcontrib>Yokoyama, Shin-ichiro</creatorcontrib><creatorcontrib>Fukao, Toshio</creatorcontrib><creatorcontrib>Yamazaki, Yoshihisa</creatorcontrib><creatorcontrib>Mori, Hiroshi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Itoh, Saori</au><au>Kariya, Masako</au><au>Nagano, Keiji</au><au>Yokoyama, Shin-ichiro</au><au>Fukao, Toshio</au><au>Yamazaki, Yoshihisa</au><au>Mori, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New Rapid Enzyme-Linked Immunosorbent Assay to Detect Antibodies against Bacterial Surface Antigens Using Filtration Plates</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2002-08-01</date><risdate>2002</risdate><volume>25</volume><issue>8</issue><spage>986</spage><epage>990</epage><pages>986-990</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>An easy and rapid ELISA system, Filtration ELISA, to detect antibodies against bacterial cell surface antigens was developed using a 96-well filtration plate fitted with a 0.22 μm membrane (MultiScreen®-GV, Millipore). Bacterial whole cells were used as antigens without fixing the cells with formalin etc. The whole cell antigens were washed by vacuum filtration through a filter and resuspended in washing buffer. Assay reactions could be done in the wells without losing the solution. The technique was established using antisera of mice immunized with Escherichia coli, and then evaluated by assaying antibodies to Shiga-toxin producing E. coli O157:H7 (STEC), Staphylococcus aureus and Lactobacillus acidophilus in fecal extracts of 157 children who had eaten school lunches contaminated with STEC in comparison with 25 age-matched control children. The lunch group showed significantly higher IgA antibody titers against STEC than the control group (p<0.0005), but not against L. acidphilus. The results indicate that Filtration ELISA is a quantitative and specific technique for measuring antibodies against antigens on the surface of bacteria without extracting antigens from the bacteria. This technique is widely applicable to the assay of antibodies in various samples including serum and fecal extract against various kinds of bacteria.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>12186431</pmid><doi>10.1248/bpb.25.986</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biological and Pharmaceutical Bulletin, 2002, Vol.25(8), pp.986-990 |
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| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Animals Antibodies - analysis antibody Antigens, Bacterial - analysis Antigens, Surface - analysis bacterial surface antigen Biological and medical sciences Biotechnology Child ELISA Enzyme-Linked Immunosorbent Assay - instrumentation Enzyme-Linked Immunosorbent Assay - methods Escherichia coli - isolation & purification feces Filtration - instrumentation Filtration - methods Food industries Food microbiology Fundamental and applied biological sciences. Psychology Genetic technics applied to diagnosis Health. Pharmaceutical industry Humans Industrial applications and implications. Economical aspects Mice O157 Shiga-toxin producing Escherichia coli |
| title | New Rapid Enzyme-Linked Immunosorbent Assay to Detect Antibodies against Bacterial Surface Antigens Using Filtration Plates |
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