Iso‐butanol saturated water: a simple procedure for increasing staining intensity of resin sections for light and electron microscopy

Summary The addition of the alcohol iso‐butanol (2‐methylpropan‐1‐ol) to water was found to improve the post‐staining procedures for semi‐thin and ultrathin resin sections, for both light and electron microscopy. Stain penetration was enhanced with samples embedded in both acrylic and epoxy resins a...

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Veröffentlicht in:	Journal of microscopy (Oxford) 2002-08, Vol.207 (2), p.97-107
1. Verfasser:	Roberts, I. M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria - ultrastructure Butanols - chemistry Electron microscopy Humans immunogold labelling In Situ Hybridization light microscopy Microscopy - methods Microscopy, Electron - methods Muscles - ultrastructure Plant Leaves - cytology Plant Leaves - virology post‐staining Resins, Plant - chemistry Staining and Labeling - methods Tissue Fixation - methods ultramicrotomy Water - chemistry
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