Endothelin content, expression, and receptor type in normal and diseased human gallbladder
The aims of this study were to characterize the endothelin (ET) system in human gallbladder by determining (1) the tissue content of ET-1 and ET-2 by ELISA; (2) the expression of mRNA of the ET precursors preproendothelin-1, -2, and -3; and (3) mRNA expression for the ETA and ETB receptors. Median c...
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| Veröffentlicht in: | Digestive diseases and sciences 2002-08, Vol.47 (8), p.1786-1792 |
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| container_title | Digestive diseases and sciences |
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| creator | MCCARTNEY, S. A GREAVES, R. R. S. H WARNER, T. D O'DONNELL, L. J. D DOMIZIO, P FARTHING, M. J. G |
| description | The aims of this study were to characterize the endothelin (ET) system in human gallbladder by determining (1) the tissue content of ET-1 and ET-2 by ELISA; (2) the expression of mRNA of the ET precursors preproendothelin-1, -2, and -3; and (3) mRNA expression for the ETA and ETB receptors. Median content of ET-1/2 was significantly reduced in severely inflamed gallbladders compared to gallbladders with mild inflammation. There was an inverse correlation between content of ET-1/2 and inflammation score. mRNA for preproendothelin-2 was highly expressed in all samples, whereas mRNA for preproendothelin-1 was present in negligible quantities and mRNA for preproendothelin-3 was undetectable. mRNA for ETA receptors was expressed in all samples analyzed, whereas mRNA for ETB receptors was expressed at a much lower level. This study demonstrates the presence of ET-1/2 in human gallbladder. ET-1/2 content is decreased with increasing degrees of histological inflammation. ET-2 is likely to be the physiologically significant endothelin isopeptide expressed and ETA receptors appear to predominate in the human gallbladder. |
| doi_str_mv | 10.1023/A:1016532228836 |
| format | Article |
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A ; GREAVES, R. R. S. H ; WARNER, T. D ; O'DONNELL, L. J. D ; DOMIZIO, P ; FARTHING, M. J. G</creator><creatorcontrib>MCCARTNEY, S. A ; GREAVES, R. R. S. H ; WARNER, T. D ; O'DONNELL, L. J. D ; DOMIZIO, P ; FARTHING, M. J. G</creatorcontrib><description>The aims of this study were to characterize the endothelin (ET) system in human gallbladder by determining (1) the tissue content of ET-1 and ET-2 by ELISA; (2) the expression of mRNA of the ET precursors preproendothelin-1, -2, and -3; and (3) mRNA expression for the ETA and ETB receptors. Median content of ET-1/2 was significantly reduced in severely inflamed gallbladders compared to gallbladders with mild inflammation. There was an inverse correlation between content of ET-1/2 and inflammation score. mRNA for preproendothelin-2 was highly expressed in all samples, whereas mRNA for preproendothelin-1 was present in negligible quantities and mRNA for preproendothelin-3 was undetectable. mRNA for ETA receptors was expressed in all samples analyzed, whereas mRNA for ETB receptors was expressed at a much lower level. This study demonstrates the presence of ET-1/2 in human gallbladder. ET-1/2 content is decreased with increasing degrees of histological inflammation. ET-2 is likely to be the physiologically significant endothelin isopeptide expressed and ETA receptors appear to predominate in the human gallbladder.</description><identifier>ISSN: 0163-2116</identifier><identifier>EISSN: 1573-2568</identifier><identifier>DOI: 10.1023/A:1016532228836</identifier><identifier>PMID: 12184531</identifier><identifier>CODEN: DDSCDJ</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Biological and medical sciences ; Cholecystitis - metabolism ; Digestive system ; Endothelin-1 - analysis ; Endothelin-1 - genetics ; Endothelin-2 - analysis ; Endothelin-2 - genetics ; Endothelins - genetics ; Enzyme-Linked Immunosorbent Assay ; Gallbladder - chemistry ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Polymerase Chain Reaction ; Protein Precursors - genetics ; Receptor, Endothelin A ; Receptor, Endothelin B ; Receptors, Endothelin - analysis ; Receptors, Endothelin - genetics ; RNA, Messenger - analysis</subject><ispartof>Digestive diseases and sciences, 2002-08, Vol.47 (8), p.1786-1792</ispartof><rights>2002 INIST-CNRS</rights><rights>Copyright Kluwer Academic Publishers Aug 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27933,27934</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13852749$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12184531$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MCCARTNEY, S. A</creatorcontrib><creatorcontrib>GREAVES, R. R. S. H</creatorcontrib><creatorcontrib>WARNER, T. D</creatorcontrib><creatorcontrib>O'DONNELL, L. J. D</creatorcontrib><creatorcontrib>DOMIZIO, P</creatorcontrib><creatorcontrib>FARTHING, M. J. G</creatorcontrib><title>Endothelin content, expression, and receptor type in normal and diseased human gallbladder</title><title>Digestive diseases and sciences</title><addtitle>Dig Dis Sci</addtitle><description>The aims of this study were to characterize the endothelin (ET) system in human gallbladder by determining (1) the tissue content of ET-1 and ET-2 by ELISA; (2) the expression of mRNA of the ET precursors preproendothelin-1, -2, and -3; and (3) mRNA expression for the ETA and ETB receptors. Median content of ET-1/2 was significantly reduced in severely inflamed gallbladders compared to gallbladders with mild inflammation. There was an inverse correlation between content of ET-1/2 and inflammation score. mRNA for preproendothelin-2 was highly expressed in all samples, whereas mRNA for preproendothelin-1 was present in negligible quantities and mRNA for preproendothelin-3 was undetectable. mRNA for ETA receptors was expressed in all samples analyzed, whereas mRNA for ETB receptors was expressed at a much lower level. This study demonstrates the presence of ET-1/2 in human gallbladder. ET-1/2 content is decreased with increasing degrees of histological inflammation. ET-2 is likely to be the physiologically significant endothelin isopeptide expressed and ETA receptors appear to predominate in the human gallbladder.</description><subject>Biological and medical sciences</subject><subject>Cholecystitis - metabolism</subject><subject>Digestive system</subject><subject>Endothelin-1 - analysis</subject><subject>Endothelin-1 - genetics</subject><subject>Endothelin-2 - analysis</subject><subject>Endothelin-2 - genetics</subject><subject>Endothelins - genetics</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Gallbladder - chemistry</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Polymerase Chain Reaction</subject><subject>Protein Precursors - genetics</subject><subject>Receptor, Endothelin A</subject><subject>Receptor, Endothelin B</subject><subject>Receptors, Endothelin - analysis</subject><subject>Receptors, Endothelin - genetics</subject><subject>RNA, Messenger - analysis</subject><issn>0163-2116</issn><issn>1573-2568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkEtLw0AUhQdRbI2u3UkQdNXoPJOpu1LqAwpudOMm3GRubMpkEmcSsP_eoBXB1T3wfRwuh5BzRm8Y5eJ2cccoS5XgnGst0gMyZSoTCVepPiTTEY2ZsXRCTkLYUkrnGUuPyYRxpqUSbEreVs60_QZt7eKydT26fhbjZ-cxhLp1sxiciT2W2PWtj_tdh_FoutY3YL-ZqQNCQBNvhgZc_A7WFhaMQX9KjiqwAc_2NyKv96uX5WOyfn54Wi7WScdT2ScAvBTaaJBUVEWWcaVSRVVFEcsiG0lllJ4LqhhFAYU0GkWpgXOjpJpLISJy_dPb-fZjwNDnTR1KtBYctkPIM04Zk2N7RC7_idt28G78LedMCqn4uElELvbSUDRo8s7XDfhd_jvZKFztBQgl2MqDK-vw5wmteCbn4gtdb3m_</recordid><startdate>20020801</startdate><enddate>20020801</enddate><creator>MCCARTNEY, S. 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J. G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p264t-aa2c38d8a403fb772556505f0eecb738dfd58930510e3ab4d8e3c8a22d5459433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Biological and medical sciences</topic><topic>Cholecystitis - metabolism</topic><topic>Digestive system</topic><topic>Endothelin-1 - analysis</topic><topic>Endothelin-1 - genetics</topic><topic>Endothelin-2 - analysis</topic><topic>Endothelin-2 - genetics</topic><topic>Endothelins - genetics</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Gallbladder - chemistry</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Polymerase Chain Reaction</topic><topic>Protein Precursors - genetics</topic><topic>Receptor, Endothelin A</topic><topic>Receptor, Endothelin B</topic><topic>Receptors, Endothelin - analysis</topic><topic>Receptors, Endothelin - genetics</topic><topic>RNA, Messenger - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MCCARTNEY, S. A</creatorcontrib><creatorcontrib>GREAVES, R. R. S. H</creatorcontrib><creatorcontrib>WARNER, T. D</creatorcontrib><creatorcontrib>O'DONNELL, L. J. D</creatorcontrib><creatorcontrib>DOMIZIO, P</creatorcontrib><creatorcontrib>FARTHING, M. J. 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A</au><au>GREAVES, R. R. S. H</au><au>WARNER, T. D</au><au>O'DONNELL, L. J. D</au><au>DOMIZIO, P</au><au>FARTHING, M. J. G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endothelin content, expression, and receptor type in normal and diseased human gallbladder</atitle><jtitle>Digestive diseases and sciences</jtitle><addtitle>Dig Dis Sci</addtitle><date>2002-08-01</date><risdate>2002</risdate><volume>47</volume><issue>8</issue><spage>1786</spage><epage>1792</epage><pages>1786-1792</pages><issn>0163-2116</issn><eissn>1573-2568</eissn><coden>DDSCDJ</coden><abstract>The aims of this study were to characterize the endothelin (ET) system in human gallbladder by determining (1) the tissue content of ET-1 and ET-2 by ELISA; (2) the expression of mRNA of the ET precursors preproendothelin-1, -2, and -3; and (3) mRNA expression for the ETA and ETB receptors. Median content of ET-1/2 was significantly reduced in severely inflamed gallbladders compared to gallbladders with mild inflammation. There was an inverse correlation between content of ET-1/2 and inflammation score. mRNA for preproendothelin-2 was highly expressed in all samples, whereas mRNA for preproendothelin-1 was present in negligible quantities and mRNA for preproendothelin-3 was undetectable. mRNA for ETA receptors was expressed in all samples analyzed, whereas mRNA for ETB receptors was expressed at a much lower level. This study demonstrates the presence of ET-1/2 in human gallbladder. ET-1/2 content is decreased with increasing degrees of histological inflammation. ET-2 is likely to be the physiologically significant endothelin isopeptide expressed and ETA receptors appear to predominate in the human gallbladder.</abstract><cop>Heidelberg</cop><pub>Springer</pub><pmid>12184531</pmid><doi>10.1023/A:1016532228836</doi><tpages>7</tpages></addata></record> |
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| subjects | Biological and medical sciences Cholecystitis - metabolism Digestive system Endothelin-1 - analysis Endothelin-1 - genetics Endothelin-2 - analysis Endothelin-2 - genetics Endothelins - genetics Enzyme-Linked Immunosorbent Assay Gallbladder - chemistry Humans Investigative techniques, diagnostic techniques (general aspects) Medical sciences Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Polymerase Chain Reaction Protein Precursors - genetics Receptor, Endothelin A Receptor, Endothelin B Receptors, Endothelin - analysis Receptors, Endothelin - genetics RNA, Messenger - analysis |
| title | Endothelin content, expression, and receptor type in normal and diseased human gallbladder |
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