Cloning and functional characterization of Phaeodactylum tricornutum front‐end desaturases involved in eicosapentaenoic acid biosynthesis

Phaeodactylum tricornutum is an unicellular silica‐less diatom in which eicosapentaenoic acid accumulates up to 30% of the total fatty acids. This marine diatom was used for cloning genes encoding fatty acid desaturases involved in eicosapentaenoic acid biosynthesis. Using a combination of PCR, mass...

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Veröffentlicht in:	European journal of biochemistry 2002-08, Vol.269 (16), p.4105-4113
Hauptverfasser:	Domergue, Frédéric, Lerchl, Jens, Zähringer, Ulrich, Heinz, Ernst
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Arachidonic Acid - biosynthesis Cloning, Molecular diatom Diatoms - enzymology Diatoms - genetics DNA, Complementary - genetics eicosapentaenoic acid Eicosapentaenoic Acid - biosynthesis Fatty Acid Desaturases - genetics Fatty Acid Desaturases - isolation & purification Fatty Acid Desaturases - physiology Fatty Acids, Omega-3 - metabolism Fatty Acids, Omega-6 Fatty Acids, Unsaturated - biosynthesis Fatty Acids, Unsaturated - metabolism front‐end desaturase Genetic Complementation Test Molecular Sequence Data Plant Proteins - genetics Plant Proteins - physiology Polymerase Chain Reaction polyunsaturated fatty acid Recombinant Fusion Proteins - metabolism Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Sequence Alignment Sequence Homology, Amino Acid Substrate Specificity
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