Development of an Enzyme-Linked Immunosorbent Assay for the Quantification of Amiodarone
A sensitive and specific enzyme-linked immunosorbent assay for an antiarrhythmic drug, amiodarone (AMI), was developed, which is capable of measuring levels as low as 16 ng/ml. Anti-AMI antibody was obtained by immunizing rabbits with an antigen conjugated with bovine serum albumin using diazotized...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2002, Vol.25(8), pp.954-958 |
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| creator | Saita, Tetsuya Fujito, Hiroshi Mori, Masato |
| description | A sensitive and specific enzyme-linked immunosorbent assay for an antiarrhythmic drug, amiodarone (AMI), was developed, which is capable of measuring levels as low as 16 ng/ml. Anti-AMI antibody was obtained by immunizing rabbits with an antigen conjugated with bovine serum albumin using diazotized 4-amino-1-(2-diethylaminoethoxy)-2,6-diiodobenzene. Enzyme labeling of AMI with β-D-galactosidase was similarly performed using a diazotized 4-amino-1-(2-diethylaminoethoxy)-2,6-diiodobenzene. This enzyme-linked immunosorbent assay was specific for AMI and showed a very slight cross-reactivity (1.25%) with its major metabolite, mono-N-desethylamiodarone. The values of the AMI concentrations measured by this assay were in good correlation to those by HPLC. Its analytical applicability was demonstrated by a kinetic study with human liver microsomes. The enzyme-linked immunosorbent assay should be a valuable tool in therapeutic drug monitoring and pharmacokinetic studies of AMI. |
| doi_str_mv | 10.1248/bpb.25.954 |
| format | Article |
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Anti-AMI antibody was obtained by immunizing rabbits with an antigen conjugated with bovine serum albumin using diazotized 4-amino-1-(2-diethylaminoethoxy)-2,6-diiodobenzene. Enzyme labeling of AMI with β-D-galactosidase was similarly performed using a diazotized 4-amino-1-(2-diethylaminoethoxy)-2,6-diiodobenzene. This enzyme-linked immunosorbent assay was specific for AMI and showed a very slight cross-reactivity (1.25%) with its major metabolite, mono-N-desethylamiodarone. The values of the AMI concentrations measured by this assay were in good correlation to those by HPLC. Its analytical applicability was demonstrated by a kinetic study with human liver microsomes. The enzyme-linked immunosorbent assay should be a valuable tool in therapeutic drug monitoring and pharmacokinetic studies of AMI.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.25.954</identifier><identifier>PMID: 12186425</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>amiodarone ; Amiodarone - analysis ; Amiodarone - chemistry ; Amiodarone - metabolism ; Analysis ; Animals ; antiarrhythmic drug ; Antiarythmic agents ; Biological and medical sciences ; Cardiovascular system ; ELISA ; Enzyme-Linked Immunosorbent Assay - methods ; Evaluation Studies as Topic ; Female ; General pharmacology ; Humans ; Medical sciences ; Microsomes, Liver ; Pharmacology. Drug treatments ; Rabbits</subject><ispartof>Biological and Pharmaceutical Bulletin, 2002, Vol.25(8), pp.954-958</ispartof><rights>2002 The Pharmaceutical Society of Japan</rights><rights>2002 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c580t-17264d4e5d3f92523683fbcaac2d831a227b49aa0d03124e87b0688ca3079ef43</citedby><cites>FETCH-LOGICAL-c580t-17264d4e5d3f92523683fbcaac2d831a227b49aa0d03124e87b0688ca3079ef43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13880405$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12186425$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saita, Tetsuya</creatorcontrib><creatorcontrib>Fujito, Hiroshi</creatorcontrib><creatorcontrib>Mori, Masato</creatorcontrib><title>Development of an Enzyme-Linked Immunosorbent Assay for the Quantification of Amiodarone</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>A sensitive and specific enzyme-linked immunosorbent assay for an antiarrhythmic drug, amiodarone (AMI), was developed, which is capable of measuring levels as low as 16 ng/ml. Anti-AMI antibody was obtained by immunizing rabbits with an antigen conjugated with bovine serum albumin using diazotized 4-amino-1-(2-diethylaminoethoxy)-2,6-diiodobenzene. Enzyme labeling of AMI with β-D-galactosidase was similarly performed using a diazotized 4-amino-1-(2-diethylaminoethoxy)-2,6-diiodobenzene. This enzyme-linked immunosorbent assay was specific for AMI and showed a very slight cross-reactivity (1.25%) with its major metabolite, mono-N-desethylamiodarone. The values of the AMI concentrations measured by this assay were in good correlation to those by HPLC. Its analytical applicability was demonstrated by a kinetic study with human liver microsomes. The enzyme-linked immunosorbent assay should be a valuable tool in therapeutic drug monitoring and pharmacokinetic studies of AMI.</description><subject>amiodarone</subject><subject>Amiodarone - analysis</subject><subject>Amiodarone - chemistry</subject><subject>Amiodarone - metabolism</subject><subject>Analysis</subject><subject>Animals</subject><subject>antiarrhythmic drug</subject><subject>Antiarythmic agents</subject><subject>Biological and medical sciences</subject><subject>Cardiovascular system</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Evaluation Studies as Topic</subject><subject>Female</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Microsomes, Liver</subject><subject>Pharmacology. Drug treatments</subject><subject>Rabbits</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0E2LFDEQBuAgijuuXvwB0iB6EHrMZ3dykmFddWFABAVvoTpd7WbsTsakWxh_vRlm3AUvqUOeqkpeQp4zumZc6rfdvltztTZKPiArJmRbK87UQ7Kihum6YUpfkCc57yilLeXiMblgnOlGcrUi39_jbxzjfsIwV3GoIFTX4c9hwnrrw0_sq5tpWkLMMXVHsckZDtUQUzXfYvVlgTD7wTuYfQzH9s3kYw8pBnxKHg0wZnx2rpfk24frr1ef6u3njzdXm23tlKZzzVreyF6i6sVguOKi0WLoHIDjvRYMOG87aQBoT0X5LOq2o43WDgRtDQ5SXJLXp7n7FH8tmGc7-exwHCFgXLJtOaVGGFPgy__gLi4plLdZJqVhRrSyKerNSbkUc0442H3yE6SDZdQe07YlbcuVLWkX_OI8cukm7O_pOd4CXp0BZAfjkCA4n--d0JpKenTvTm6XZ_iBdwDS7N2I_3bq01FW3924W0gWg_gLrw2dhg</recordid><startdate>20020801</startdate><enddate>20020801</enddate><creator>Saita, Tetsuya</creator><creator>Fujito, Hiroshi</creator><creator>Mori, Masato</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20020801</creationdate><title>Development of an Enzyme-Linked Immunosorbent Assay for the Quantification of Amiodarone</title><author>Saita, Tetsuya ; Fujito, Hiroshi ; Mori, Masato</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c580t-17264d4e5d3f92523683fbcaac2d831a227b49aa0d03124e87b0688ca3079ef43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>amiodarone</topic><topic>Amiodarone - analysis</topic><topic>Amiodarone - chemistry</topic><topic>Amiodarone - metabolism</topic><topic>Analysis</topic><topic>Animals</topic><topic>antiarrhythmic drug</topic><topic>Antiarythmic agents</topic><topic>Biological and medical sciences</topic><topic>Cardiovascular system</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Evaluation Studies as Topic</topic><topic>Female</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Microsomes, Liver</topic><topic>Pharmacology. Drug treatments</topic><topic>Rabbits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saita, Tetsuya</creatorcontrib><creatorcontrib>Fujito, Hiroshi</creatorcontrib><creatorcontrib>Mori, Masato</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saita, Tetsuya</au><au>Fujito, Hiroshi</au><au>Mori, Masato</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of an Enzyme-Linked Immunosorbent Assay for the Quantification of Amiodarone</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2002-08-01</date><risdate>2002</risdate><volume>25</volume><issue>8</issue><spage>954</spage><epage>958</epage><pages>954-958</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>A sensitive and specific enzyme-linked immunosorbent assay for an antiarrhythmic drug, amiodarone (AMI), was developed, which is capable of measuring levels as low as 16 ng/ml. Anti-AMI antibody was obtained by immunizing rabbits with an antigen conjugated with bovine serum albumin using diazotized 4-amino-1-(2-diethylaminoethoxy)-2,6-diiodobenzene. Enzyme labeling of AMI with β-D-galactosidase was similarly performed using a diazotized 4-amino-1-(2-diethylaminoethoxy)-2,6-diiodobenzene. This enzyme-linked immunosorbent assay was specific for AMI and showed a very slight cross-reactivity (1.25%) with its major metabolite, mono-N-desethylamiodarone. The values of the AMI concentrations measured by this assay were in good correlation to those by HPLC. Its analytical applicability was demonstrated by a kinetic study with human liver microsomes. The enzyme-linked immunosorbent assay should be a valuable tool in therapeutic drug monitoring and pharmacokinetic studies of AMI.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>12186425</pmid><doi>10.1248/bpb.25.954</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | amiodarone Amiodarone - analysis Amiodarone - chemistry Amiodarone - metabolism Analysis Animals antiarrhythmic drug Antiarythmic agents Biological and medical sciences Cardiovascular system ELISA Enzyme-Linked Immunosorbent Assay - methods Evaluation Studies as Topic Female General pharmacology Humans Medical sciences Microsomes, Liver Pharmacology. Drug treatments Rabbits |
| title | Development of an Enzyme-Linked Immunosorbent Assay for the Quantification of Amiodarone |
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