Cellular uptake of antisense oligonucleotides after complexing or conjugation with cell‐penetrating model peptides

The uptake by mammalian cells of phosphorothioate oligonucleotides was compared with that of their respective complexes or conjugates with cationic, cell‐penetrating model peptides of varying helix‐forming propensity and amphipathicity. An HPLC‐based protocol for the synthesis and purification of di...

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Veröffentlicht in:	European journal of biochemistry 2002-08, Vol.269 (16), p.4025-4032
Hauptverfasser:	Oehlke, J., Birth, P., Klauschenz, E., Wiesner, B., Beyermann, M., Oksche, A., Bienert, M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Aorta Cell Membrane Permeability Cell Nucleus - metabolism cellular uptake cell‐penetrating peptides CHO Cells Chromatography, High Pressure Liquid Cricetinae Cricetulus Cystine - chemistry Cytoplasm - metabolism Dogs Electrophoresis, Capillary Endothelium, Vascular - cytology Endothelium, Vascular - metabolism Fluorometry Microfilament Proteins - genetics Microscopy, Confocal Molecular Sequence Data Oligodeoxyribonucleotides, Antisense - metabolism oligonucleotide‐peptide conjugates Peptides - chemical synthesis Peptides - chemistry Peptides - metabolism Thionucleotides - metabolism
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