Identification of two novel markers for alveolar epithelial type I and II cells
Alveolar epithelial type I and type II cells (AEC I and II) are closely aligned in alveolar surface. There is much interest in the precise identification of AEC I and II in order to separate and evaluate functional and other properties of these two cells. This study aims to identify specific AEC I a...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2004-07, Vol.319 (3), p.774-780 |
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| creator | Chen, Zhongming Jin, Nili Narasaraju, Telugu Chen, Jiwang McFarland, Lucas R. Scott, Mary Liu, Lin |
| description | Alveolar epithelial type I and type II cells (AEC I and II) are closely aligned in alveolar surface. There is much interest in the precise identification of AEC I and II in order to separate and evaluate functional and other properties of these two cells. This study aims to identify specific AEC I and AEC II cell markers by DNA microarray using the in vitro trans-differentiation of AEC II into AEC I-like cells as a model. Quantitative real-time PCR confirmed five AEC I genes: fibroblast growth factor receptor-activating protein 1, aquaporin 5, purinergic receptor P2X 7 (P2X7), interferon-induced protein, and Bcl2-associated protein, and one AEC II gene: γ-aminobutyric acid receptor pi subunit (GABRP). Immunostaining on cultured cells and rat lung tissue indicated that GABRP and P2X7 proteins were specifically expressed in AEC II and AEC I, respectively. In situ hybridization of rat lung tissue confirmed the localization of GABRP mRNA in type II cells. P2X7 and GABRP identified in this study could be used as potential AEC I and AEC II markers for studying lung epithelial cell biology and monitoring lung injury. |
| doi_str_mv | 10.1016/j.bbrc.2004.05.048 |
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There is much interest in the precise identification of AEC I and II in order to separate and evaluate functional and other properties of these two cells. This study aims to identify specific AEC I and AEC II cell markers by DNA microarray using the in vitro trans-differentiation of AEC II into AEC I-like cells as a model. Quantitative real-time PCR confirmed five AEC I genes: fibroblast growth factor receptor-activating protein 1, aquaporin 5, purinergic receptor P2X 7 (P2X7), interferon-induced protein, and Bcl2-associated protein, and one AEC II gene: γ-aminobutyric acid receptor pi subunit (GABRP). Immunostaining on cultured cells and rat lung tissue indicated that GABRP and P2X7 proteins were specifically expressed in AEC II and AEC I, respectively. In situ hybridization of rat lung tissue confirmed the localization of GABRP mRNA in type II cells. P2X7 and GABRP identified in this study could be used as potential AEC I and AEC II markers for studying lung epithelial cell biology and monitoring lung injury.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2004.05.048</identifier><identifier>PMID: 15184050</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Alveolar epithelial cells ; Animals ; Biomarkers ; Cell differentiation ; Cell marker ; Cells, Cultured ; Epithelial Cells - classification ; Epithelial Cells - cytology ; Epithelial Cells - physiology ; Gene Expression Profiling ; Immunohistochemistry ; In Situ Hybridization ; Lung injury ; Male ; Oligonucleotide Array Sequence Analysis ; Protein Subunits - genetics ; Protein Subunits - metabolism ; Pulmonary Alveoli - cytology ; Pulmonary Alveoli - metabolism ; Rats ; Rats, Sprague-Dawley ; Receptors, GABA - genetics ; Receptors, GABA - metabolism ; Receptors, Purinergic P2 - genetics ; Receptors, Purinergic P2 - metabolism ; Receptors, Purinergic P2X7 ; Respiratory Mucosa - cytology ; Respiratory Mucosa - metabolism</subject><ispartof>Biochemical and biophysical research communications, 2004-07, Vol.319 (3), p.774-780</ispartof><rights>2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-20dbf06784062f6b2d4f9526d4c56b6e6a3a8dcaec913666f5b5771747a91f5d3</citedby><cites>FETCH-LOGICAL-c418t-20dbf06784062f6b2d4f9526d4c56b6e6a3a8dcaec913666f5b5771747a91f5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X04010058$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15184050$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Zhongming</creatorcontrib><creatorcontrib>Jin, Nili</creatorcontrib><creatorcontrib>Narasaraju, Telugu</creatorcontrib><creatorcontrib>Chen, Jiwang</creatorcontrib><creatorcontrib>McFarland, Lucas R.</creatorcontrib><creatorcontrib>Scott, Mary</creatorcontrib><creatorcontrib>Liu, Lin</creatorcontrib><title>Identification of two novel markers for alveolar epithelial type I and II cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Alveolar epithelial type I and type II cells (AEC I and II) are closely aligned in alveolar surface. There is much interest in the precise identification of AEC I and II in order to separate and evaluate functional and other properties of these two cells. This study aims to identify specific AEC I and AEC II cell markers by DNA microarray using the in vitro trans-differentiation of AEC II into AEC I-like cells as a model. Quantitative real-time PCR confirmed five AEC I genes: fibroblast growth factor receptor-activating protein 1, aquaporin 5, purinergic receptor P2X 7 (P2X7), interferon-induced protein, and Bcl2-associated protein, and one AEC II gene: γ-aminobutyric acid receptor pi subunit (GABRP). Immunostaining on cultured cells and rat lung tissue indicated that GABRP and P2X7 proteins were specifically expressed in AEC II and AEC I, respectively. In situ hybridization of rat lung tissue confirmed the localization of GABRP mRNA in type II cells. P2X7 and GABRP identified in this study could be used as potential AEC I and AEC II markers for studying lung epithelial cell biology and monitoring lung injury.</description><subject>Alveolar epithelial cells</subject><subject>Animals</subject><subject>Biomarkers</subject><subject>Cell differentiation</subject><subject>Cell marker</subject><subject>Cells, Cultured</subject><subject>Epithelial Cells - classification</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - physiology</subject><subject>Gene Expression Profiling</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Lung injury</subject><subject>Male</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Protein Subunits - genetics</subject><subject>Protein Subunits - metabolism</subject><subject>Pulmonary Alveoli - cytology</subject><subject>Pulmonary Alveoli - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, GABA - genetics</subject><subject>Receptors, GABA - metabolism</subject><subject>Receptors, Purinergic P2 - genetics</subject><subject>Receptors, Purinergic P2 - metabolism</subject><subject>Receptors, Purinergic P2X7</subject><subject>Respiratory Mucosa - cytology</subject><subject>Respiratory Mucosa - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLAzEUhYMoWh9_wIVk5W7GmzTJdMCNiI-BghsFdyGT3GDqdFKTacV_75QW3Lm6m-8ezvkIuWRQMmDqZlG2bbIlBxAlyBLE7IBMGNRQcAbikEwAQBW8Zu8n5DTnBQBjQtXH5IRJNhMgYUJeGof9EHywZgixp9HT4TvSPm6wo0uTPjFl6mOipttg7EyiuArDB3bBdHT4WSFtqOkdbRpqsevyOTnypst4sb9n5O3x4fX-uZi_PDX3d_PCCjYbCg6u9aCqsYXiXrXcCV9LrpywUrUKlZmambMGbc2mSikvW1lVrBKVqZmXbnpGrne5qxS_1pgHvQx528D0GNdZV6MVxYCPIN-BNsWcE3q9SmEc9qMZ6K1GvdBbjXqrUYPUo8bx6Wqfvm6X6P5e9t5G4HYH4LhxEzDpbAP2Fl1IaAftYvgv_xcro4L-</recordid><startdate>20040702</startdate><enddate>20040702</enddate><creator>Chen, Zhongming</creator><creator>Jin, Nili</creator><creator>Narasaraju, Telugu</creator><creator>Chen, Jiwang</creator><creator>McFarland, Lucas R.</creator><creator>Scott, Mary</creator><creator>Liu, Lin</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040702</creationdate><title>Identification of two novel markers for alveolar epithelial type I and II cells</title><author>Chen, Zhongming ; Jin, Nili ; Narasaraju, Telugu ; Chen, Jiwang ; McFarland, Lucas R. ; Scott, Mary ; Liu, Lin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-20dbf06784062f6b2d4f9526d4c56b6e6a3a8dcaec913666f5b5771747a91f5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Alveolar epithelial cells</topic><topic>Animals</topic><topic>Biomarkers</topic><topic>Cell differentiation</topic><topic>Cell marker</topic><topic>Cells, Cultured</topic><topic>Epithelial Cells - classification</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - physiology</topic><topic>Gene Expression Profiling</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Lung injury</topic><topic>Male</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Protein Subunits - genetics</topic><topic>Protein Subunits - metabolism</topic><topic>Pulmonary Alveoli - cytology</topic><topic>Pulmonary Alveoli - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, GABA - genetics</topic><topic>Receptors, GABA - metabolism</topic><topic>Receptors, Purinergic P2 - genetics</topic><topic>Receptors, Purinergic P2 - metabolism</topic><topic>Receptors, Purinergic P2X7</topic><topic>Respiratory Mucosa - cytology</topic><topic>Respiratory Mucosa - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Zhongming</creatorcontrib><creatorcontrib>Jin, Nili</creatorcontrib><creatorcontrib>Narasaraju, Telugu</creatorcontrib><creatorcontrib>Chen, Jiwang</creatorcontrib><creatorcontrib>McFarland, Lucas R.</creatorcontrib><creatorcontrib>Scott, Mary</creatorcontrib><creatorcontrib>Liu, Lin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Zhongming</au><au>Jin, Nili</au><au>Narasaraju, Telugu</au><au>Chen, Jiwang</au><au>McFarland, Lucas R.</au><au>Scott, Mary</au><au>Liu, Lin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of two novel markers for alveolar epithelial type I and II cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2004-07-02</date><risdate>2004</risdate><volume>319</volume><issue>3</issue><spage>774</spage><epage>780</epage><pages>774-780</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Alveolar epithelial type I and type II cells (AEC I and II) are closely aligned in alveolar surface. There is much interest in the precise identification of AEC I and II in order to separate and evaluate functional and other properties of these two cells. This study aims to identify specific AEC I and AEC II cell markers by DNA microarray using the in vitro trans-differentiation of AEC II into AEC I-like cells as a model. Quantitative real-time PCR confirmed five AEC I genes: fibroblast growth factor receptor-activating protein 1, aquaporin 5, purinergic receptor P2X 7 (P2X7), interferon-induced protein, and Bcl2-associated protein, and one AEC II gene: γ-aminobutyric acid receptor pi subunit (GABRP). Immunostaining on cultured cells and rat lung tissue indicated that GABRP and P2X7 proteins were specifically expressed in AEC II and AEC I, respectively. In situ hybridization of rat lung tissue confirmed the localization of GABRP mRNA in type II cells. P2X7 and GABRP identified in this study could be used as potential AEC I and AEC II markers for studying lung epithelial cell biology and monitoring lung injury.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15184050</pmid><doi>10.1016/j.bbrc.2004.05.048</doi><tpages>7</tpages></addata></record> |
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| subjects | Alveolar epithelial cells Animals Biomarkers Cell differentiation Cell marker Cells, Cultured Epithelial Cells - classification Epithelial Cells - cytology Epithelial Cells - physiology Gene Expression Profiling Immunohistochemistry In Situ Hybridization Lung injury Male Oligonucleotide Array Sequence Analysis Protein Subunits - genetics Protein Subunits - metabolism Pulmonary Alveoli - cytology Pulmonary Alveoli - metabolism Rats Rats, Sprague-Dawley Receptors, GABA - genetics Receptors, GABA - metabolism Receptors, Purinergic P2 - genetics Receptors, Purinergic P2 - metabolism Receptors, Purinergic P2X7 Respiratory Mucosa - cytology Respiratory Mucosa - metabolism |
| title | Identification of two novel markers for alveolar epithelial type I and II cells |
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