Conserved neutralizing epitopes of HIV type 1 CRF01―AE against primary isolates in long-term nonprogressors
Linear conserved B cell epitopes in envelope glycoprotein of long-term nonprogressors (LTNPs) HIV-1 CRF01_AE were determined. The envelope sequences of HIV-1 subtype E from Thailand were aligned to define consensus sequences. Then the peptides corresponding to these predicted regions were synthesize...
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| Veröffentlicht in: | AIDS research and human retroviruses 2004-05, Vol.20 (5), p.531-542 |
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| creator | SREEPIAN, Apichai SRISURAPANON, Surangrat HORTHONGKHAM, Nawin TUNSUPASAWASDIKUL, Somsith KAORIANGUDOM, Surapol KHUSMITH, Srisin SUTTHENT, Ruengpung |
| description | Linear conserved B cell epitopes in envelope glycoprotein of long-term nonprogressors (LTNPs) HIV-1 CRF01_AE were determined. The envelope sequences of HIV-1 subtype E from Thailand were aligned to define consensus sequences. Then the peptides corresponding to these predicted regions were synthesized as peptides represent C1, C2, C3, C5, V2, V3, and gp41 regions. After that, the neutralizing B cell epitopes were determined by neutralized competitive assay with pool sera of typical progressor and LTNP HIV-1 CRF01_AE patients against HIV-1 CRF01_AE 24 primary isolates (PI) and laboratory strains (TCLA). We found that the strength and breadth of neutralization were greater for sera from LTNPs compared with sera from typical progressors. Peptides C1E and C2E could inhibit primary isolates but not the TCLA strain in LTNP sera. The new B cell epitopes, which were located in the C1 and C2 regions of CRF01_AE against primary HIV-1 isolates, were identified in HIV-1 CRF01_AE LTNPs. This may be important in HIV-1 vaccine development and trial. |
| doi_str_mv | 10.1089/088922204323087787 |
| format | Article |
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The envelope sequences of HIV-1 subtype E from Thailand were aligned to define consensus sequences. Then the peptides corresponding to these predicted regions were synthesized as peptides represent C1, C2, C3, C5, V2, V3, and gp41 regions. After that, the neutralizing B cell epitopes were determined by neutralized competitive assay with pool sera of typical progressor and LTNP HIV-1 CRF01_AE patients against HIV-1 CRF01_AE 24 primary isolates (PI) and laboratory strains (TCLA). We found that the strength and breadth of neutralization were greater for sera from LTNPs compared with sera from typical progressors. Peptides C1E and C2E could inhibit primary isolates but not the TCLA strain in LTNP sera. The new B cell epitopes, which were located in the C1 and C2 regions of CRF01_AE against primary HIV-1 isolates, were identified in HIV-1 CRF01_AE LTNPs. This may be important in HIV-1 vaccine development and trial.</description><identifier>ISSN: 0889-2229</identifier><identifier>EISSN: 1931-8405</identifier><identifier>DOI: 10.1089/088922204323087787</identifier><identifier>PMID: 15186528</identifier><identifier>CODEN: ARHRE7</identifier><language>eng</language><publisher>Larchmont, NY: Liebert</publisher><subject>AIDS/HIV ; Amino Acid Sequence ; B-Lymphocytes - immunology ; Biological and medical sciences ; Disease Progression ; Enzyme-Linked Immunosorbent Assay ; Epitopes - immunology ; Fundamental and applied biological sciences. Psychology ; HIV Infections - immunology ; HIV Infections - virology ; HIV Long-Term Survivors ; HIV-1 - immunology ; Human immunodeficiency virus 1 ; Human viral diseases ; Humans ; Infectious diseases ; Medical sciences ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; Neutralization Tests ; Viral diseases ; Viral diseases of the lymphoid tissue and the blood. Aids ; Virology</subject><ispartof>AIDS research and human retroviruses, 2004-05, Vol.20 (5), p.531-542</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-28cbc1f76c390fffc65afe7cc0bccd9c4cda3d8f434b6af2e31fb43e0bbf170c3</citedby><cites>FETCH-LOGICAL-c360t-28cbc1f76c390fffc65afe7cc0bccd9c4cda3d8f434b6af2e31fb43e0bbf170c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,3029,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15770432$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15186528$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SREEPIAN, Apichai</creatorcontrib><creatorcontrib>SRISURAPANON, Surangrat</creatorcontrib><creatorcontrib>HORTHONGKHAM, Nawin</creatorcontrib><creatorcontrib>TUNSUPASAWASDIKUL, Somsith</creatorcontrib><creatorcontrib>KAORIANGUDOM, Surapol</creatorcontrib><creatorcontrib>KHUSMITH, Srisin</creatorcontrib><creatorcontrib>SUTTHENT, Ruengpung</creatorcontrib><title>Conserved neutralizing epitopes of HIV type 1 CRF01―AE against primary isolates in long-term nonprogressors</title><title>AIDS research and human retroviruses</title><addtitle>AIDS Res Hum Retroviruses</addtitle><description>Linear conserved B cell epitopes in envelope glycoprotein of long-term nonprogressors (LTNPs) HIV-1 CRF01_AE were determined. The envelope sequences of HIV-1 subtype E from Thailand were aligned to define consensus sequences. Then the peptides corresponding to these predicted regions were synthesized as peptides represent C1, C2, C3, C5, V2, V3, and gp41 regions. After that, the neutralizing B cell epitopes were determined by neutralized competitive assay with pool sera of typical progressor and LTNP HIV-1 CRF01_AE patients against HIV-1 CRF01_AE 24 primary isolates (PI) and laboratory strains (TCLA). We found that the strength and breadth of neutralization were greater for sera from LTNPs compared with sera from typical progressors. Peptides C1E and C2E could inhibit primary isolates but not the TCLA strain in LTNP sera. The new B cell epitopes, which were located in the C1 and C2 regions of CRF01_AE against primary HIV-1 isolates, were identified in HIV-1 CRF01_AE LTNPs. This may be important in HIV-1 vaccine development and trial.</description><subject>AIDS/HIV</subject><subject>Amino Acid Sequence</subject><subject>B-Lymphocytes - immunology</subject><subject>Biological and medical sciences</subject><subject>Disease Progression</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitopes - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HIV Infections - immunology</subject><subject>HIV Infections - virology</subject><subject>HIV Long-Term Survivors</subject><subject>HIV-1 - immunology</subject><subject>Human immunodeficiency virus 1</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Neutralization Tests</subject><subject>Viral diseases</subject><subject>Viral diseases of the lymphoid tissue and the blood. Aids</subject><subject>Virology</subject><issn>0889-2229</issn><issn>1931-8405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c1q3TAQBWBRWpqbtC_QRdGm3TkdSbYlL8MlfxAolLZbI8uji4otORrfQLrKS_QF-yR1EkMCWXSlzXfOMBrGPgg4FmCaL2BMI6WEUkkFRmujX7GNaJQoTAnVa7a5B8UimgN2SPQLABZfvWUHohKmrqTZsHGbImG-wZ5H3M_ZDuF3iDuOU5jThMST5xeXP_l8OyEXfPvtDMTfuz8np9zubIg08ymH0eZbHigNdl4SIfIhxV0xYx55THHKaZeRKGV6x954OxC-X98j9uPs9Pv2orj6en65PbkqnKphLqRxnRNe10414L13dWU9auegc65vXOl6q3rjS1V2tfUSlfBdqRC6zgsNTh2xz4-9y-zrPdLcjoEcDoONmPbUagmgdFn_FwrdNFLpaoHyEbqciDL6dt27FdDeX6N9eY0l9HFt33cj9k-R9fsX8GkFlpwdfLbRBXrmtH7o-wdsg5S1</recordid><startdate>20040501</startdate><enddate>20040501</enddate><creator>SREEPIAN, Apichai</creator><creator>SRISURAPANON, Surangrat</creator><creator>HORTHONGKHAM, Nawin</creator><creator>TUNSUPASAWASDIKUL, Somsith</creator><creator>KAORIANGUDOM, Surapol</creator><creator>KHUSMITH, Srisin</creator><creator>SUTTHENT, Ruengpung</creator><general>Liebert</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20040501</creationdate><title>Conserved neutralizing epitopes of HIV type 1 CRF01―AE against primary isolates in long-term nonprogressors</title><author>SREEPIAN, Apichai ; SRISURAPANON, Surangrat ; HORTHONGKHAM, Nawin ; TUNSUPASAWASDIKUL, Somsith ; KAORIANGUDOM, Surapol ; KHUSMITH, Srisin ; SUTTHENT, Ruengpung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-28cbc1f76c390fffc65afe7cc0bccd9c4cda3d8f434b6af2e31fb43e0bbf170c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>AIDS/HIV</topic><topic>Amino Acid Sequence</topic><topic>B-Lymphocytes - immunology</topic><topic>Biological and medical sciences</topic><topic>Disease Progression</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitopes - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HIV Infections - immunology</topic><topic>HIV Infections - virology</topic><topic>HIV Long-Term Survivors</topic><topic>HIV-1 - immunology</topic><topic>Human immunodeficiency virus 1</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Neutralization Tests</topic><topic>Viral diseases</topic><topic>Viral diseases of the lymphoid tissue and the blood. Aids</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SREEPIAN, Apichai</creatorcontrib><creatorcontrib>SRISURAPANON, Surangrat</creatorcontrib><creatorcontrib>HORTHONGKHAM, Nawin</creatorcontrib><creatorcontrib>TUNSUPASAWASDIKUL, Somsith</creatorcontrib><creatorcontrib>KAORIANGUDOM, Surapol</creatorcontrib><creatorcontrib>KHUSMITH, Srisin</creatorcontrib><creatorcontrib>SUTTHENT, Ruengpung</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>AIDS research and human retroviruses</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SREEPIAN, Apichai</au><au>SRISURAPANON, Surangrat</au><au>HORTHONGKHAM, Nawin</au><au>TUNSUPASAWASDIKUL, Somsith</au><au>KAORIANGUDOM, Surapol</au><au>KHUSMITH, Srisin</au><au>SUTTHENT, Ruengpung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Conserved neutralizing epitopes of HIV type 1 CRF01―AE against primary isolates in long-term nonprogressors</atitle><jtitle>AIDS research and human retroviruses</jtitle><addtitle>AIDS Res Hum Retroviruses</addtitle><date>2004-05-01</date><risdate>2004</risdate><volume>20</volume><issue>5</issue><spage>531</spage><epage>542</epage><pages>531-542</pages><issn>0889-2229</issn><eissn>1931-8405</eissn><coden>ARHRE7</coden><abstract>Linear conserved B cell epitopes in envelope glycoprotein of long-term nonprogressors (LTNPs) HIV-1 CRF01_AE were determined. The envelope sequences of HIV-1 subtype E from Thailand were aligned to define consensus sequences. Then the peptides corresponding to these predicted regions were synthesized as peptides represent C1, C2, C3, C5, V2, V3, and gp41 regions. After that, the neutralizing B cell epitopes were determined by neutralized competitive assay with pool sera of typical progressor and LTNP HIV-1 CRF01_AE patients against HIV-1 CRF01_AE 24 primary isolates (PI) and laboratory strains (TCLA). We found that the strength and breadth of neutralization were greater for sera from LTNPs compared with sera from typical progressors. Peptides C1E and C2E could inhibit primary isolates but not the TCLA strain in LTNP sera. The new B cell epitopes, which were located in the C1 and C2 regions of CRF01_AE against primary HIV-1 isolates, were identified in HIV-1 CRF01_AE LTNPs. This may be important in HIV-1 vaccine development and trial.</abstract><cop>Larchmont, NY</cop><pub>Liebert</pub><pmid>15186528</pmid><doi>10.1089/088922204323087787</doi><tpages>12</tpages></addata></record> |
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| ispartof | AIDS research and human retroviruses, 2004-05, Vol.20 (5), p.531-542 |
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| source | Mary Ann Liebert Online Subscription; MEDLINE; Alma/SFX Local Collection |
| subjects | AIDS/HIV Amino Acid Sequence B-Lymphocytes - immunology Biological and medical sciences Disease Progression Enzyme-Linked Immunosorbent Assay Epitopes - immunology Fundamental and applied biological sciences. Psychology HIV Infections - immunology HIV Infections - virology HIV Long-Term Survivors HIV-1 - immunology Human immunodeficiency virus 1 Human viral diseases Humans Infectious diseases Medical sciences Microbiology Miscellaneous Molecular Sequence Data Neutralization Tests Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids Virology |
| title | Conserved neutralizing epitopes of HIV type 1 CRF01―AE against primary isolates in long-term nonprogressors |
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