Müller cell response to laser-induced increase in intraocular pressure in rats
The goal of this study was to investigate the reaction of the Müller cells to elevated intraocular pressure (IOP). Elevated IOP is one of the risk factors in glaucomatous retinal ganglion cell (RGC) degeneration. Müller cells play an important role in retinal homeostasis. The reaction of Müller cell...
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| Veröffentlicht in: | Glia 2004-08, Vol.47 (2), p.109-119 |
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| description | The goal of this study was to investigate the reaction of the Müller cells to elevated intraocular pressure (IOP). Elevated IOP is one of the risk factors in glaucomatous retinal ganglion cell (RGC) degeneration. Müller cells play an important role in retinal homeostasis. The reaction of Müller cells was examined by evaluating temporal changes in glutamate aspartate transporter (GLAST), glutamine synthase (GS), glial fibrillary acidic protein (GFAP), and the B‐cell lymphoma (Bcl‐2) using immunoblotting and immunohistochemical techniques. After IOP was elevated for 4–60 days, there was a time‐related decrease in RGC ranging from 6% to 44%. There was also a time‐related increase in GLAST protein reaching maximum after 3 weeks of elevated IOP. On the other hand, there was very little change in the expression of GS during the first 2 weeks followed by some increase between 21 and 60 days. An increase in Bcl‐2 was biphasic with maximum increase after 4 days followed by decline after 15 and 21 days. GFAP, which is usually not expressed in normal Müller cells, was present at all time points. In all cases, the increase was most intense in the vicinity of the ganglion cells where the astrocytes and endfeet of the Müller cells are located. These results indicate that Müller cells react to the insult of elevated IOP by expressing GFAP and Bcl‐2, proteins that are expressed in reactive gliosis and other pathological conditions. The increase in GLAST along with minimum change in GS indicates a disturbance in glutamate homeostasis. © 2004 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/glia.20000 |
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Elevated IOP is one of the risk factors in glaucomatous retinal ganglion cell (RGC) degeneration. Müller cells play an important role in retinal homeostasis. The reaction of Müller cells was examined by evaluating temporal changes in glutamate aspartate transporter (GLAST), glutamine synthase (GS), glial fibrillary acidic protein (GFAP), and the B‐cell lymphoma (Bcl‐2) using immunoblotting and immunohistochemical techniques. After IOP was elevated for 4–60 days, there was a time‐related decrease in RGC ranging from 6% to 44%. There was also a time‐related increase in GLAST protein reaching maximum after 3 weeks of elevated IOP. On the other hand, there was very little change in the expression of GS during the first 2 weeks followed by some increase between 21 and 60 days. An increase in Bcl‐2 was biphasic with maximum increase after 4 days followed by decline after 15 and 21 days. GFAP, which is usually not expressed in normal Müller cells, was present at all time points. In all cases, the increase was most intense in the vicinity of the ganglion cells where the astrocytes and endfeet of the Müller cells are located. These results indicate that Müller cells react to the insult of elevated IOP by expressing GFAP and Bcl‐2, proteins that are expressed in reactive gliosis and other pathological conditions. The increase in GLAST along with minimum change in GS indicates a disturbance in glutamate homeostasis. © 2004 Wiley‐Liss, Inc.</description><identifier>ISSN: 0894-1491</identifier><identifier>EISSN: 1098-1136</identifier><identifier>DOI: 10.1002/glia.20000</identifier><identifier>PMID: 15185390</identifier><identifier>CODEN: GLIAEJ</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Amino Acid Transport System X-AG - metabolism ; Animals ; Biological and medical sciences ; Cell Count ; Disease Models, Animal ; Down-Regulation - physiology ; Down-Regulation - radiation effects ; Eye and associated structures. Visual pathways and centers. Vision ; Fundamental and applied biological sciences. Psychology ; ganglion cells ; Glaucoma - metabolism ; Glaucoma - pathology ; Glaucoma - physiopathology ; Glial Fibrillary Acidic Protein - metabolism ; Glutamate-Ammonia Ligase - metabolism ; Glutamic Acid - metabolism ; Homeostasis - physiology ; Homeostasis - radiation effects ; immunoreactivity ; Intraocular Pressure - physiology ; Intraocular Pressure - radiation effects ; Isolated neuron and nerve. Neuroglia ; laser photocoagulation ; Lasers - adverse effects ; Male ; Müller cells ; Neuroglia - metabolism ; Neuroglia - pathology ; Neuroglia - radiation effects ; ocular hypertension ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Rats ; Rats, Wistar ; Reaction Time - physiology ; Reaction Time - radiation effects ; Retina - metabolism ; Retina - pathology ; Retina - physiopathology ; Retinal Degeneration - etiology ; Retinal Degeneration - pathology ; Retinal Degeneration - physiopathology ; Retinal Ganglion Cells - pathology ; Vertebrates: nervous system and sense organs</subject><ispartof>Glia, 2004-08, Vol.47 (2), p.109-119</ispartof><rights>Copyright © 2004 Wiley‐Liss, Inc.</rights><rights>2004 INIST-CNRS</rights><rights>Copyright 2004 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3930-2dcf513e3bcfca74a8e430f8d62fe902eb68238ebb7266cbc00b05dc3dc986ea3</citedby><cites>FETCH-LOGICAL-c3930-2dcf513e3bcfca74a8e430f8d62fe902eb68238ebb7266cbc00b05dc3dc986ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fglia.20000$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fglia.20000$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15949622$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15185390$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Woldemussie, Elizabeth</creatorcontrib><creatorcontrib>Wijono, Mercy</creatorcontrib><creatorcontrib>Ruiz, Guadalupe</creatorcontrib><title>Müller cell response to laser-induced increase in intraocular pressure in rats</title><title>Glia</title><addtitle>Glia</addtitle><description>The goal of this study was to investigate the reaction of the Müller cells to elevated intraocular pressure (IOP). Elevated IOP is one of the risk factors in glaucomatous retinal ganglion cell (RGC) degeneration. Müller cells play an important role in retinal homeostasis. The reaction of Müller cells was examined by evaluating temporal changes in glutamate aspartate transporter (GLAST), glutamine synthase (GS), glial fibrillary acidic protein (GFAP), and the B‐cell lymphoma (Bcl‐2) using immunoblotting and immunohistochemical techniques. After IOP was elevated for 4–60 days, there was a time‐related decrease in RGC ranging from 6% to 44%. There was also a time‐related increase in GLAST protein reaching maximum after 3 weeks of elevated IOP. On the other hand, there was very little change in the expression of GS during the first 2 weeks followed by some increase between 21 and 60 days. An increase in Bcl‐2 was biphasic with maximum increase after 4 days followed by decline after 15 and 21 days. GFAP, which is usually not expressed in normal Müller cells, was present at all time points. In all cases, the increase was most intense in the vicinity of the ganglion cells where the astrocytes and endfeet of the Müller cells are located. These results indicate that Müller cells react to the insult of elevated IOP by expressing GFAP and Bcl‐2, proteins that are expressed in reactive gliosis and other pathological conditions. The increase in GLAST along with minimum change in GS indicates a disturbance in glutamate homeostasis. © 2004 Wiley‐Liss, Inc.</description><subject>Amino Acid Transport System X-AG - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Disease Models, Animal</subject><subject>Down-Regulation - physiology</subject><subject>Down-Regulation - radiation effects</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>ganglion cells</subject><subject>Glaucoma - metabolism</subject><subject>Glaucoma - pathology</subject><subject>Glaucoma - physiopathology</subject><subject>Glial Fibrillary Acidic Protein - metabolism</subject><subject>Glutamate-Ammonia Ligase - metabolism</subject><subject>Glutamic Acid - metabolism</subject><subject>Homeostasis - physiology</subject><subject>Homeostasis - radiation effects</subject><subject>immunoreactivity</subject><subject>Intraocular Pressure - physiology</subject><subject>Intraocular Pressure - radiation effects</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>laser photocoagulation</subject><subject>Lasers - adverse effects</subject><subject>Male</subject><subject>Müller cells</subject><subject>Neuroglia - metabolism</subject><subject>Neuroglia - pathology</subject><subject>Neuroglia - radiation effects</subject><subject>ocular hypertension</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reaction Time - physiology</subject><subject>Reaction Time - radiation effects</subject><subject>Retina - metabolism</subject><subject>Retina - pathology</subject><subject>Retina - physiopathology</subject><subject>Retinal Degeneration - etiology</subject><subject>Retinal Degeneration - pathology</subject><subject>Retinal Degeneration - physiopathology</subject><subject>Retinal Ganglion Cells - pathology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0894-1491</issn><issn>1098-1136</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKxDAUhoMoOo5ufADpRhdC9STpLUsRHS-jo6AIbkKanko1045Ji_pu7nwxMxcvK0MgJ4fv_w_nJ2SLwj4FYAePplL7DPxZIj0KIgsp5cky6UEmopBGgq6RdeeeAKj_pKtkjcY0i7mAHhldfn4YgzbQaExg0U2a2mHQNoFRDm1Y1UWnsQiqWlv0HV_421rV6M4oG0y8xHV21reqdRtkpVTG4ebi7ZO7k-Pbo9NwOBqcHR0OQ80Fh5AVuowpR57rUqs0UhlGHMqsSFiJAhjmScZ4hnmesiTRuQbIIS40L7TIElS8T3bnvhPbvHToWjmu3HQHVWPTOZnO8gDhwb05qG3jnMVSTmw1VvZdUpDT-OQ0PjnDPby9cO3yMRa_6CIvD-wsAOW0MqVVta7cH05EImHMc3TOvVYG3_8ZKQfDs8Pv4eFcU7kW3340yj7LJOVpLO-vBvKG3z-cxxfXcsi_AM46mCs</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Woldemussie, Elizabeth</creator><creator>Wijono, Mercy</creator><creator>Ruiz, Guadalupe</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>Müller cell response to laser-induced increase in intraocular pressure in rats</title><author>Woldemussie, Elizabeth ; Wijono, Mercy ; Ruiz, Guadalupe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3930-2dcf513e3bcfca74a8e430f8d62fe902eb68238ebb7266cbc00b05dc3dc986ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Transport System X-AG - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Count</topic><topic>Disease Models, Animal</topic><topic>Down-Regulation - physiology</topic><topic>Down-Regulation - radiation effects</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>ganglion cells</topic><topic>Glaucoma - metabolism</topic><topic>Glaucoma - pathology</topic><topic>Glaucoma - physiopathology</topic><topic>Glial Fibrillary Acidic Protein - metabolism</topic><topic>Glutamate-Ammonia Ligase - metabolism</topic><topic>Glutamic Acid - metabolism</topic><topic>Homeostasis - physiology</topic><topic>Homeostasis - radiation effects</topic><topic>immunoreactivity</topic><topic>Intraocular Pressure - physiology</topic><topic>Intraocular Pressure - radiation effects</topic><topic>Isolated neuron and nerve. Neuroglia</topic><topic>laser photocoagulation</topic><topic>Lasers - adverse effects</topic><topic>Male</topic><topic>Müller cells</topic><topic>Neuroglia - metabolism</topic><topic>Neuroglia - pathology</topic><topic>Neuroglia - radiation effects</topic><topic>ocular hypertension</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reaction Time - physiology</topic><topic>Reaction Time - radiation effects</topic><topic>Retina - metabolism</topic><topic>Retina - pathology</topic><topic>Retina - physiopathology</topic><topic>Retinal Degeneration - etiology</topic><topic>Retinal Degeneration - pathology</topic><topic>Retinal Degeneration - physiopathology</topic><topic>Retinal Ganglion Cells - pathology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Woldemussie, Elizabeth</creatorcontrib><creatorcontrib>Wijono, Mercy</creatorcontrib><creatorcontrib>Ruiz, Guadalupe</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Glia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Woldemussie, Elizabeth</au><au>Wijono, Mercy</au><au>Ruiz, Guadalupe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Müller cell response to laser-induced increase in intraocular pressure in rats</atitle><jtitle>Glia</jtitle><addtitle>Glia</addtitle><date>2004-08-01</date><risdate>2004</risdate><volume>47</volume><issue>2</issue><spage>109</spage><epage>119</epage><pages>109-119</pages><issn>0894-1491</issn><eissn>1098-1136</eissn><coden>GLIAEJ</coden><abstract>The goal of this study was to investigate the reaction of the Müller cells to elevated intraocular pressure (IOP). Elevated IOP is one of the risk factors in glaucomatous retinal ganglion cell (RGC) degeneration. Müller cells play an important role in retinal homeostasis. The reaction of Müller cells was examined by evaluating temporal changes in glutamate aspartate transporter (GLAST), glutamine synthase (GS), glial fibrillary acidic protein (GFAP), and the B‐cell lymphoma (Bcl‐2) using immunoblotting and immunohistochemical techniques. After IOP was elevated for 4–60 days, there was a time‐related decrease in RGC ranging from 6% to 44%. There was also a time‐related increase in GLAST protein reaching maximum after 3 weeks of elevated IOP. On the other hand, there was very little change in the expression of GS during the first 2 weeks followed by some increase between 21 and 60 days. An increase in Bcl‐2 was biphasic with maximum increase after 4 days followed by decline after 15 and 21 days. GFAP, which is usually not expressed in normal Müller cells, was present at all time points. In all cases, the increase was most intense in the vicinity of the ganglion cells where the astrocytes and endfeet of the Müller cells are located. These results indicate that Müller cells react to the insult of elevated IOP by expressing GFAP and Bcl‐2, proteins that are expressed in reactive gliosis and other pathological conditions. The increase in GLAST along with minimum change in GS indicates a disturbance in glutamate homeostasis. © 2004 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>15185390</pmid><doi>10.1002/glia.20000</doi><tpages>11</tpages></addata></record> |
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| subjects | Amino Acid Transport System X-AG - metabolism Animals Biological and medical sciences Cell Count Disease Models, Animal Down-Regulation - physiology Down-Regulation - radiation effects Eye and associated structures. Visual pathways and centers. Vision Fundamental and applied biological sciences. Psychology ganglion cells Glaucoma - metabolism Glaucoma - pathology Glaucoma - physiopathology Glial Fibrillary Acidic Protein - metabolism Glutamate-Ammonia Ligase - metabolism Glutamic Acid - metabolism Homeostasis - physiology Homeostasis - radiation effects immunoreactivity Intraocular Pressure - physiology Intraocular Pressure - radiation effects Isolated neuron and nerve. Neuroglia laser photocoagulation Lasers - adverse effects Male Müller cells Neuroglia - metabolism Neuroglia - pathology Neuroglia - radiation effects ocular hypertension Proto-Oncogene Proteins c-bcl-2 - metabolism Rats Rats, Wistar Reaction Time - physiology Reaction Time - radiation effects Retina - metabolism Retina - pathology Retina - physiopathology Retinal Degeneration - etiology Retinal Degeneration - pathology Retinal Degeneration - physiopathology Retinal Ganglion Cells - pathology Vertebrates: nervous system and sense organs |
| title | Müller cell response to laser-induced increase in intraocular pressure in rats |
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