NELL2 promotes motor and sensory neuron differentiation and stimulates mitogenesis in DRG in vivo
We previously identified a secreted glycoprotein, neural epidermal growth factor- like like 2 (NELL2), in a subtraction screen designed to identify molecules regulating sensory neurogenesis and differentiation in the chick dorsal root ganglion (DRG). Characterization of NELL2 expression during embry...
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| Veröffentlicht in: | Developmental biology 2004-06, Vol.270 (2), p.322-335 |
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| creator | Nelson, Branden R Claes, Karen Todd, Valerie Chaverra, Marta Lefcort, Frances |
| description | We previously identified a secreted glycoprotein,
neural
epidermal growth factor-
like
like
2 (NELL2), in a subtraction screen designed to identify molecules regulating sensory neurogenesis and differentiation in the chick dorsal root ganglion (DRG). Characterization of NELL2 expression during embryogenesis revealed that NELL2 was specifically expressed during the peak periods of both sensory and motor neuron differentiation, and within the neural crest was restricted to the sensory lineage. We now provide evidence for a function for NELL2 during neuronal development. We report here that NELL2 acts cell autonomously within CNS and PNS progenitors, in vivo, to promote their differentiation into neurons. Additionally, neuron-secreted NELL2 acts paracrinely to stimulate the mitogenesis of adjacent cells within the nascent DRG. These studies implicate dual functions for NELL2 in both the cell autonomous differentiation of neural progenitor cells while simultaneously exerting paracrine proliferative activity. |
| doi_str_mv | 10.1016/j.ydbio.2004.03.004 |
| format | Article |
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neural
epidermal growth factor-
like
like
2 (NELL2), in a subtraction screen designed to identify molecules regulating sensory neurogenesis and differentiation in the chick dorsal root ganglion (DRG). Characterization of NELL2 expression during embryogenesis revealed that NELL2 was specifically expressed during the peak periods of both sensory and motor neuron differentiation, and within the neural crest was restricted to the sensory lineage. We now provide evidence for a function for NELL2 during neuronal development. We report here that NELL2 acts cell autonomously within CNS and PNS progenitors, in vivo, to promote their differentiation into neurons. Additionally, neuron-secreted NELL2 acts paracrinely to stimulate the mitogenesis of adjacent cells within the nascent DRG. These studies implicate dual functions for NELL2 in both the cell autonomous differentiation of neural progenitor cells while simultaneously exerting paracrine proliferative activity.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1016/j.ydbio.2004.03.004</identifier><identifier>PMID: 15183717</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Differentiation - physiology ; Chick Embryo ; Electrophoresis, Polyacrylamide Gel ; Ganglia, Spinal - cytology ; Ganglia, Spinal - embryology ; Gene Expression Regulation, Developmental ; Immunohistochemistry ; In ovo electroporation ; In Situ Hybridization ; Mitosis - physiology ; Motor neuron ; NELL2 ; Nerve Tissue Proteins - physiology ; Neural Crest - embryology ; Neural crest cells ; Neuronal differentiation ; Neurons - physiology ; Plasmids - genetics ; Proliferation ; Secreted glycoprotein ; Sensory neuron ; Transfection</subject><ispartof>Developmental biology, 2004-06, Vol.270 (2), p.322-335</ispartof><rights>2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-6c5e38b60cd8e3ed4c49d5c1bf850ca7dcb9eaeef0ea38dcda1bbf9a4adbe6a73</citedby><cites>FETCH-LOGICAL-c431t-6c5e38b60cd8e3ed4c49d5c1bf850ca7dcb9eaeef0ea38dcda1bbf9a4adbe6a73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ydbio.2004.03.004$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15183717$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nelson, Branden R</creatorcontrib><creatorcontrib>Claes, Karen</creatorcontrib><creatorcontrib>Todd, Valerie</creatorcontrib><creatorcontrib>Chaverra, Marta</creatorcontrib><creatorcontrib>Lefcort, Frances</creatorcontrib><title>NELL2 promotes motor and sensory neuron differentiation and stimulates mitogenesis in DRG in vivo</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>We previously identified a secreted glycoprotein,
neural
epidermal growth factor-
like
like
2 (NELL2), in a subtraction screen designed to identify molecules regulating sensory neurogenesis and differentiation in the chick dorsal root ganglion (DRG). Characterization of NELL2 expression during embryogenesis revealed that NELL2 was specifically expressed during the peak periods of both sensory and motor neuron differentiation, and within the neural crest was restricted to the sensory lineage. We now provide evidence for a function for NELL2 during neuronal development. We report here that NELL2 acts cell autonomously within CNS and PNS progenitors, in vivo, to promote their differentiation into neurons. Additionally, neuron-secreted NELL2 acts paracrinely to stimulate the mitogenesis of adjacent cells within the nascent DRG. These studies implicate dual functions for NELL2 in both the cell autonomous differentiation of neural progenitor cells while simultaneously exerting paracrine proliferative activity.</description><subject>Animals</subject><subject>Cell Differentiation - physiology</subject><subject>Chick Embryo</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Ganglia, Spinal - cytology</subject><subject>Ganglia, Spinal - embryology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Immunohistochemistry</subject><subject>In ovo electroporation</subject><subject>In Situ Hybridization</subject><subject>Mitosis - physiology</subject><subject>Motor neuron</subject><subject>NELL2</subject><subject>Nerve Tissue Proteins - physiology</subject><subject>Neural Crest - embryology</subject><subject>Neural crest cells</subject><subject>Neuronal differentiation</subject><subject>Neurons - physiology</subject><subject>Plasmids - genetics</subject><subject>Proliferation</subject><subject>Secreted glycoprotein</subject><subject>Sensory neuron</subject><subject>Transfection</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9r3DAQxUVpyW42-QSF4lNvdkaWZcuHHkKy2QSWFkoLuQlZGhcta2kr2Qv77aP9A7m1l3kM_N4MM4-QzxQKCrS-2xQH01lflABVAaxI8oHMKbQ853X1-pHMAWiZ0xrqGbmOcQMATAh2RWaUU8Ea2syJ-r5cr8tsF_zgR4xZqj5kypksoos-HDKHU_AuM7bvMaAbrRpt6k_IaIdpq04-O_o_6DDamFmXPf5cHWVv9_6GfOrVNuLtRRfk99Py18Nzvv6xenm4X-e6YnTMa82Ria4GbQQyNJWuWsM17XrBQavG6K5FhdgDKiaMNop2Xd-qSpkOa9WwBfl6nptu-TthHOVgo8btVjn0U5QNbVtegvgvSBtRUs7bBLIzqIOPMWAvd8EOKhwkBXmMQG7kKQJ5jEACk0mS68tl_NQNaN49l58n4NsZwPSNvcUgo7boNBobUI_SePvPBW93VJvP</recordid><startdate>20040615</startdate><enddate>20040615</enddate><creator>Nelson, Branden R</creator><creator>Claes, Karen</creator><creator>Todd, Valerie</creator><creator>Chaverra, Marta</creator><creator>Lefcort, Frances</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20040615</creationdate><title>NELL2 promotes motor and sensory neuron differentiation and stimulates mitogenesis in DRG in vivo</title><author>Nelson, Branden R ; Claes, Karen ; Todd, Valerie ; Chaverra, Marta ; Lefcort, Frances</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-6c5e38b60cd8e3ed4c49d5c1bf850ca7dcb9eaeef0ea38dcda1bbf9a4adbe6a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Cell Differentiation - physiology</topic><topic>Chick Embryo</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Ganglia, Spinal - cytology</topic><topic>Ganglia, Spinal - embryology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Immunohistochemistry</topic><topic>In ovo electroporation</topic><topic>In Situ Hybridization</topic><topic>Mitosis - physiology</topic><topic>Motor neuron</topic><topic>NELL2</topic><topic>Nerve Tissue Proteins - physiology</topic><topic>Neural Crest - embryology</topic><topic>Neural crest cells</topic><topic>Neuronal differentiation</topic><topic>Neurons - physiology</topic><topic>Plasmids - genetics</topic><topic>Proliferation</topic><topic>Secreted glycoprotein</topic><topic>Sensory neuron</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nelson, Branden R</creatorcontrib><creatorcontrib>Claes, Karen</creatorcontrib><creatorcontrib>Todd, Valerie</creatorcontrib><creatorcontrib>Chaverra, Marta</creatorcontrib><creatorcontrib>Lefcort, Frances</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nelson, Branden R</au><au>Claes, Karen</au><au>Todd, Valerie</au><au>Chaverra, Marta</au><au>Lefcort, Frances</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>NELL2 promotes motor and sensory neuron differentiation and stimulates mitogenesis in DRG in vivo</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>2004-06-15</date><risdate>2004</risdate><volume>270</volume><issue>2</issue><spage>322</spage><epage>335</epage><pages>322-335</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><abstract>We previously identified a secreted glycoprotein,
neural
epidermal growth factor-
like
like
2 (NELL2), in a subtraction screen designed to identify molecules regulating sensory neurogenesis and differentiation in the chick dorsal root ganglion (DRG). Characterization of NELL2 expression during embryogenesis revealed that NELL2 was specifically expressed during the peak periods of both sensory and motor neuron differentiation, and within the neural crest was restricted to the sensory lineage. We now provide evidence for a function for NELL2 during neuronal development. We report here that NELL2 acts cell autonomously within CNS and PNS progenitors, in vivo, to promote their differentiation into neurons. Additionally, neuron-secreted NELL2 acts paracrinely to stimulate the mitogenesis of adjacent cells within the nascent DRG. These studies implicate dual functions for NELL2 in both the cell autonomous differentiation of neural progenitor cells while simultaneously exerting paracrine proliferative activity.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15183717</pmid><doi>10.1016/j.ydbio.2004.03.004</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Developmental biology, 2004-06, Vol.270 (2), p.322-335 |
| issn | 0012-1606 1095-564X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71995208 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Cell Differentiation - physiology Chick Embryo Electrophoresis, Polyacrylamide Gel Ganglia, Spinal - cytology Ganglia, Spinal - embryology Gene Expression Regulation, Developmental Immunohistochemistry In ovo electroporation In Situ Hybridization Mitosis - physiology Motor neuron NELL2 Nerve Tissue Proteins - physiology Neural Crest - embryology Neural crest cells Neuronal differentiation Neurons - physiology Plasmids - genetics Proliferation Secreted glycoprotein Sensory neuron Transfection |
| title | NELL2 promotes motor and sensory neuron differentiation and stimulates mitogenesis in DRG in vivo |
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